Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,075,528 C→T A1119T (GCA→ACA)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,075,5280CT98.4% 178.2 / NA 62A1119T (GCA→ACA) putAfused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (29/32);  total (30/32)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

GCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCT  >  NC_000913/1075282‑1075767
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
gCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAg                                                                                                                                                                                                                                              <  2:216042/250‑1 (MQ=255)
 cTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGCGCGTCACTGTCAgcg                                                                                                                                                                                                                                            >  2:494928/1‑251 (MQ=255)
      catcGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCGGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCTATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACtt                                                                                                                                                                                                                                       <  1:43702/251‑1 (MQ=255)
              aTACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCg                                                                                                                                                                                                                               <  1:149037/251‑1 (MQ=255)
                acacAACACGCGCGCACGGGGCAGCAGCGGCCAGGTGTTGCGTCCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCGGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTATCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCTTCACTGTCAGCGCACTTTCCGGGCGAt                                                                                                                                                                                                                             <  2:526399/251‑1 (MQ=255)
                      cacGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCca                                                                                                                                                                                                                       <  1:62358/251‑1 (MQ=255)
                         taatatgatgggCAGCAGCGTCCAGGTGCTGCATTCACCCGTCGACCCCGGCCGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGCCGATTTGCTGCCCATTCCCGCAGTGCATTTCGCGGCTGAGTCAATGCGGCTTTCAACTGCGCCTCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGcccgc                                                                                                                                                                                                                     >  2:365542/11‑247 (MQ=255)
                           acTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATAGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCcagc                                                                                                                                                                                                                     >  2:118132/2‑248 (MQ=255)
                                        cagcGTTCAGGTGTTGCGTTCACCCGTCGGTCCCGGCAGCAATCGTTGTGTCCCTGCCTGCGCTAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATaga                                                                                                                                                                                                     <  1:379818/251‑1 (MQ=255)
                                         agcttccagtttgtGCGGTCACCCGTCGGCCCCGTCAGCAAGCGTTGGGTTCCTGCCTGCGCCAGCGTGCCATATTGCGTACATAACTCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTGTCAACTGCGCAGCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGTCGATTCTCCAGCAGACGGTAGAGATagag                                                                                                                                                                                                    <  2:529490/238‑1 (MQ=255)
                                            gTCCAGGTTTTGCGTTCCCCCGTCGGCCCCTGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCgg                                                                                                                                                                                                 <  2:96582/251‑1 (MQ=255)
                                            gTCCAAGTGATGCGTTCACCCGTCGGCCCCGACAGCAATCGTAGTGTTCCTGCCTGCACCACCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCAGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGCTACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCCCTTTCCGGGCGATTCGCCAGCAGACAGTAGAGACAGAGCgg                                                                                                                                                                                                 >  2:576717/1‑251 (MQ=255)
                                              ccAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGCGCggcc                                                                                                                                                                                               >  1:277446/1‑249 (MQ=255)
                                                  gtgtTGCGTTCTCCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGcc                                                                                                                                                                                           <  1:23003/251‑1 (MQ=255)
                                                       gCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCtt                                                                                                                                                                                      <  1:337351/251‑1 (MQ=255)
                                                       gCGTTCAACCGTCGGCCCCGGCAGCAATCGTTGGGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATGCGCCAGCAGACGGTAGAGATAGCGCGGACCGCCTGCtt                                                                                                                                                                                      >  2:453509/1‑251 (MQ=255)
                                                          ttCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCg                                                                                                                                                                                   >  1:456935/1‑251 (MQ=255)
                                                                   cGGCCCCTGCAGCAATCGTTGTGTTTCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGAGTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTAc                                                                                                                                                                          <  2:457312/251‑1 (MQ=255)
                                                                      ccccGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCgg                                                                                                                                                                       <  2:489683/251‑1 (MQ=255)
                                                                       cccGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCggg                                                                                                                                                                      >  1:81608/1‑250 (MQ=255)
                                                                            cagcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAAcc                                                                                                                                                                 >  1:55452/1‑251 (MQ=255)
                                                                             ggcaATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTGCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTATCGGACAAccc                                                                                                                                                                <  2:267071/250‑1 (MQ=255)
                                                                                aaTCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTc                                                                                                                                                             <  2:308324/251‑1 (MQ=255)
                                                                                   cGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcgcc                                                                                                                                                          <  1:394673/251‑1 (MQ=255)
                                                                                    gTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTcgccg                                                                                                                                                         <  1:454421/251‑1 (MQ=255)
                                                                                            cctgcctgCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACg                                                                                                                                                  <  2:310875/250‑1 (MQ=255)
                                                                                              tgcctgcGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCt                                                                                                                                               >  1:52262/1‑251 (MQ=255)
                                                                                               gcctgcGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGAACACCCTTCGCCGCCGAACGGCTg                                                                                                                                              >  2:126796/1‑251 (MQ=255)
                                                                                                       cAGCTCGCCATATTGCGTACATAACGCCGGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACaccaa                                                                                                                                      <  2:477958/251‑1 (MQ=255)
                                                                                                        aGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACaccaac                                                                                                                                     <  2:122615/251‑1 (MQ=255)
                                                                                                            cGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACaccaaccccc                                                                                                                                 >  2:336174/1‑248 (MQ=255)
                                                                                                               cATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTgcg                                                                                                                              <  1:494928/251‑1 (MQ=255)
                                                                                                                atatTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTgcgc                                                                                                                             >  1:100404/1‑251 (MQ=255)
                                                                                                                    tGCGTACATAACTCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGAAAACCCTTCGCCGCCGAACGGCTGCCCACCACCCCCTACGCccac                                                                                                                         >  2:431864/1‑251 (MQ=255)
                                                                                                                      cGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTc                                                                                                                                                                                    <  1:150855/190‑1 (MQ=255)
                                                                                                                      cGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTc                                                                                                                                                                                    >  2:150855/1‑190 (MQ=255)
                                                                                                                         aCATAGCGCCTGCAATTCTAGACGATTTGCTGCCCCTTCCCGCAGAGCATTTAGCGACTGAGCCAATGAGGCTTTCAACTGCGCATCGACCGGATACTTTGCATACTGACGCGCGACCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGACAGAGGAGACCGCCGCCTTTCTGCCCGGGCCCGGACACCCCTTCACTGCCGCACGGCTGCACGCCAACCACCGCCCCCCCCatat                                                                                                                    >  2:481920/1‑251 (MQ=255)
                                                                                                                             aaCGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACGGCGCATCGACCGGATACTTTGCAGCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACTGCTGCACACCAACCACTGCGCCCTCCATATTa                                                                                                                  <  1:365542/249‑1 (MQ=255)
                                                                                                                              aCGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACgg                                                                                                               >  1:310881/1‑251 (MQ=255)
                                                                                                                                    gCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAAc                                                                                                          >  1:307686/1‑250 (MQ=255)
                                                                                                                                     cAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACAt                                                                                                        >  2:467512/1‑251 (MQ=255)
                                                                                                                                           tGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGTTTCGCCAGAAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCATCGCCGCCGAACGGCTGCACACCAACCACTTCGCCCACCatataaaggtgaacacaccgtt                                                                                                  >  2:499233/1‑233 (MQ=255)
                                                                                                                                              aCGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTAc                                                                                               >  1:30384/1‑251 (MQ=255)
                                                                                                                                               cGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTAcc                                                                                              >  1:424270/1‑251 (MQ=255)
                                                                                                                                                               cccGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGcc                                                                               >  1:492267/1‑250 (MQ=255)
                                                                                                                                                                       gCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCt                                                                       <  1:566333/250‑1 (MQ=255)
                                                                                                                                                                                      gTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCAATTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGCCAACCCTTCGCCGCCGAACGGCTGCACACCACCAACTGCGCCCACCATATTACGGTTAACCTACAGGTTACCAACATGGCCCAAAACAGTGCCCTGGGCGATGGTTtcatc                                                       >  1:613341/1‑251 (MQ=255)
                                                                                                                                                                                          aTGCGGCGTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCCCTTGCCGGGCGATTCGCCAGCATACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTGTCATCAATg                                                   <  2:46203/251‑1 (MQ=255)
                                                                                                                                                                                                  ttCAACTGCGCATCGCCCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTAt                                            <  2:203114/250‑1 (MQ=255)
                                                                                                                                                                                                  ttCAACGGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTa                                             <  1:237427/249‑1 (MQ=255)
                                                                                                                                                                                                         gcgcATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGc                                      <  1:590996/249‑1 (MQ=255)
                                                                                                                                                                                                         gcgcATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATAGTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAAGGCGCGTATGGACGCCa                                    <  1:541927/251‑1 (MQ=255)
                                                                                                                                                                                                            cATCGACCGCATCCTTTGCATCCAGCCGCGCGAGCGCCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGCCGGAAGCGATAGAGCGGACCGCCTGCTTTCGGCCCGGAACCGGACACCCCATCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGCGCCAGTGACCTGGGCGATGGTTTCATCCATGCGCGTATGGACGCCAAGc                                 >  2:319437/1‑251 (MQ=255)
                                                                                                                                                                                                                gACCGGATTCTTTGCATCCTGACGCGCGATCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGAGGGTTTCATCAATGCGCGTATGGACGCCAATCGTCa                             <  2:290491/251‑1 (MQ=255)
                                                                                                                                                                                                                 aCCGGATACTTTGCATCCTGACGCGCGAGCGTCCCTGTCAGCGCCCTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACACCCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTTCGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAg                            <  1:57172/251‑1 (MQ=255)
                                                                                                                                                                                                                     gATACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGACTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCACCATGGGCCGCGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAACGTCCGAccc                        >  1:224982/1‑250 (MQ=255)
                                                                                                                                                                                                                       tACTTTGCATCCTGACGCGCGAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCAt                       >  1:259597/1‑250 (MQ=255)
                                                                                                                                                                                                                        aCTTTGCATCCTGACGCGCGAGCGTAACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGCACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGGTGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATa                      >  2:254067/1‑250 (MQ=255)
                                                                                                                                                                                                                            tGCATCCTGACGCGCGAGAGTCACTGTCAGCGCACTTTCCGGGCGCTTCCCCAGCAGACGGTAGAGATAGCGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCAACCATATTAAGGTTAACATACAGGTTAACAAAATAGACCAACACAGAGACCTGGGAGATGGATTCATCAATGCGCGTATGGACGCAAAGCGTCAGACCATAACCgg                 >  1:301197/1‑251 (MQ=255)
                                                                                                                                                                                                                                 ccTGACGCGCGAGCATCCCTGTCAGCGCACTTTACGGGCGATTCGCCAGCAGAAGGTAGAGATAGAGCGGACCGCCTGATTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGACGAGCCAGTGACCTGGGCGCTGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCCGACAATAACCGGAAGCg            >  2:558604/1‑251 (MQ=255)
                                                                                                                                                                                                                                           gAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGCCCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCt  <  2:472345/251‑1 (MQ=255)
                                                                                                                                                                                                                                           gAGCGTCACTGTCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCt  <  1:254348/251‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                               
GCTCATCATCGGCAATACACAACACGCGCTCACGCGGCAGCAGCGTCCAGGTGTTGCGTTCACCCGTCGGCCCCGGCAGCAATCGTTGTGTTCCTGCCTGCGCCAGCTCGCCATATTGCGTACATAACGCCTGCAATTCTGGACGATTTGCTGCCCATTCCCGCAGTGCATTTAGCGGCTGAGTCAATGCGGCTTTCAACTGCGCATCGACCGGATACTTTGCATCCTGACGCGCGAGCGTCACTGCCAGCGCACTTTCCGGGCGATTCGCCAGCAGACGGTAGAGATAGAGCGGACCGCCTGCTTTCGGCCCGGTACCGGACAACCCTTCGCCGCCGAACGGCTGCACACCAACCACTGCGCCCACCATATTACGGTTAACATACAGGTTACCAACATGGGCCGAGCCAGTGACCTGGGCGATGGTTTCATCAATGCGCGTATGGACGCCAAGCGTCAGACCATAACCGGAAGCGTTAATCTGCT  >  NC_000913/1075282‑1075767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑