Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,001,882	G→A	A26T (GCT→ACT)	elfG →	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,001,882	0	G	A	100.0%	100.5 / NA	33	A26T (GCT→ACT)	elfG	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/19);  total (14/19)

TACAGGCTAATAGCGGCATAATTGATGATGATGGCTCGCTCTATATGGCGGGTTTACCGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCGCTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGAC  >  NC_000913/1001635‑1002121                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                tACAGGCTAATAGCGGCATAATTGATGATGATGGCTCGCTCTATATGGCGGGTTTACCGGCGAAGGGAACAATAAGCGGGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTAAGCGAATGGGTGCCCTATGTAGATAATATTTGGAGAAAAATTCGGGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTc                                                                                                                                                                                                                                              >  2:250881/1‑251 (MQ=255)            aGCGGCATAATTGATGATGATGGCTCGCTCTATATGGCGGGTTTACCGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGCCTATTTTTTGCCGCCGTCTTAATGTTCTGGTGCACTCAAAcc                                                                                                                                                                                                                                         >  2:500705/1‑245 (MQ=255)            aGCGGCATAATTGATGATGATGGCTCGCTCTATATAGCGGGTTTACCGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAAcc                                                                                                                                                                                                                                         <  1:500705/245‑1 (MQ=255)                       tgatgatgaTGGCTCGCTCTATATGGCGGGTTTACCGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGc                                                                                                                                                                                                                         <  1:331542/250‑1 (MQ=255)                                           atatGGCGGGTTTACCGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGGGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAgggg                                                                                                                                                                                                     >  2:575773/1‑250 (MQ=255)                                                   ggTTTACCGGCGAAGGGAACCATAAGCGTGCGCTGGGGCTAAGCGCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGtat                                                                                                                                                                                            <  2:228834/251‑1 (MQ=255)                                                         ccGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGc                                                                                                                                                                                      <  1:38517/251‑1 (MQ=255)                                                                               tGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAAccc                                                                                                                                                                >  1:294469/1‑251 (MQ=255)                                                                                         cGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGgaaga                                                                                                                                                      <  1:334307/251‑1 (MQ=255)                                                                                            aGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGc                                                                                                                                                   <  1:188648/251‑1 (MQ=255)                                                                                                  cGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGTAGGAAGAAGCAAATAc                                                                                                                                             <  1:185683/251‑1 (MQ=255)                                                                                                           ttGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAgggg                                                                                                                                     <  1:250881/250‑1 (MQ=255)                                                                                                                atatCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTa                                                                                                                               <  2:448658/251‑1 (MQ=255)                                                                                                                  agcAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATAGTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTaa                                                                                                                              <  2:503360/248‑1 (MQ=255)                                                                                                                        tACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACaa                                                                                                                        >  1:22359/1‑250 (MQ=255)                                                                                                                        tACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACaa                                                                                                                        >  1:15210/1‑250 (MQ=255)                                                                                                                           gAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAAACAAAttt                                                                                                                    >  2:175112/1‑251 (MQ=255)                                                                                                                               ttACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATc                                                                                                                >  1:383413/1‑251 (MQ=255)                                                                                                                                                             cgagAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGttt                                                                                  <  1:544183/248‑1 (MQ=255)                                                                                                                                                                gAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGGCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCt                                                                               <  2:628550/251‑1 (MQ=255)                                                                                                                                                                 aaTGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTGTGGCGGTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGACCGAATCGAATGATTATTATGTTTCCtg                                                                              <  1:394256/251‑1 (MQ=255)                                                                                                                                                                                               gaAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTgg                                                <  2:22359/251‑1 (MQ=255)                                                                                                                                                                                                      gCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATgg                                         <  2:294469/251‑1 (MQ=255)                                                                                                                                                                                                            cATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTGCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCAtt                                    <  2:292103/250‑1 (MQ=255)                                                                                                                                                                                                             aTTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAACCCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGTAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGCATGATTATTATGTTTCCTGTGATTGAGGTAAAGACAATGTCAGAAGTGGCCGATGGGcctt                                    >  1:559830/1‑246 (MQ=255)                                                                                                                                                                                                                 ctacGACTATTTTTTGCCGCCGTCTTAATGTTATGGGGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAAACAATGTCAGAAGGGGCCGAGGGGCATTcgcagc                              >  2:242125/1‑248 (MQ=255)                                                                                                                                                                                                                     gACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGTTGCTTATAGCGGGCAGTGTCATAATACTCAGGAGAATCCGTATCTTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTCGTTAAAGACAAATTTTATCAGTGGAACGAATCGCATGATTATTATGTTTCCTGTGATTGCGATCAAGACAATGTCACAAGTGGCCTATTGGCATTTTCCGCGGAt                          >  2:413702/1‑251 (MQ=255)                                                                                                                                                                                                                            ttttGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCACTTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGAAAAATTTTATCAGGGGAAc                                                                                                        >  2:190164/1‑166 (MQ=255)                                                                                                                                                                                                                            ttttGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCATGGGTAGTTAAAGACAAATGTTAGCAGTGGAAc                                                                                                        <  1:190164/166‑1 (MQ=255)                                                                                                                                                                                                                               tGCCGCCGTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTa                 <  1:575773/250‑1 (MQ=255)                                                                                                                                                                                                                                    ccgTCTTAATGTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTAt           >  1:347331/1‑251 (MQ=255)                                                                                                                                                                                                                                     cgTCTTTATGTTATGGTTCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGGCAATTGTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGAGGCCGATTGGCATTCGCCGCGGATTTGCCGTTAGTCTAtt          <  2:434688/251‑1 (MQ=255)                                                                                                                                                                                                                                              gTTATGGTGCACTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTTAAGACAAATTTTGTCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGACGTGGCCGGTGGGCATTCGCCGCGGATTCAACGTTAGTCTATTTAGGAGAc  >  2:137340/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                TACAGGCTAATAGCGGCATAATTGATGATGATGGCTCGCTCTATATGGCGGGTTTACCGGCGAAGGGAACAATAAGCGTGCGCTGGGGCGAAGCTCCCGATCAAATTTGTCATATCAATTACGAGCTTACCGAACAACAAATTAACTCTGCGATTACGCGAATGGATGCCATATGCAGATAATCTTTGGAGAAAAATGCGTGTCATTACTACGACTATTTTTTGCCGCCGTCTTAATGTTATGGTGCGCTCAAACCGCTGCTTATAGCGGGCAGTGTCATACTACTCAGGGGAATCCGTATATTGGCGTCAATTTTGGCGTTAAAACCCTGGAGGAAGAAGCAAATACGGCAGGGGTAGTTAAAGACAAATTTTATCAGTGGAACGAATCGAATGATTATTATGTTTCCTGTGATTGCGATAAAGACAATGTCAGAAGTGGCCGATGGGCATTCGCCGCGGATTCACCGTTAGTCTATTTAGGCGAC  >  NC_000913/1001635‑1002121

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑