Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 882,731 C→A E2* (GAA→TAA)  deoR ← DNA‑binding transcriptional repressor DeoR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913882,7310CA94.0% 143.2 / NA 50E2* (GAA→TAA) deoRDNA‑binding transcriptional repressor DeoR
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (24/23);  total (27/23)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGC  >  NC_000913/882490‑882975
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
gccCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATccc                                                                                                                                                                                                                                             <  1:262101/251‑1 (MQ=255)
 cccGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCct                                                                                                                                                                                                                                            >  1:410238/1‑251 (MQ=255)
  ccGGCGTTTTTCTTCCACCAGGCGGGATTTTTGCTCGCTTAACAGGTACTGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCctc                                                                                                                                                                                                                                           >  2:611812/1‑251 (MQ=255)
  ccGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCctc                                                                                                                                                                                                                                           <  2:474738/251‑1 (MQ=255)
                ccaccaGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTg                                                                                                                                                                                                                              >  2:233090/1‑250 (MQ=255)
                    caGGCGGGCTTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTCACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGCCGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCg                                                                                                                                                                                                                         >  2:142452/1‑251 (MQ=255)
                           gATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCt                                                                                                                                                                                                                  >  1:65528/1‑251 (MQ=255)
                              ttttGATCGCGTAACAGGGAATGGCAGGCACTGAGCGGTTCGAAAACAAGATAGCCGCCGAGAAAAACGACGGGGGCACTGTGGTTGGTAAGATCGCGACGCATCGTCAGCTCCGAAACCCGAAGCAGGGCGGCGGCGTATTGAAGAGGTATCTGATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCTGATAAGCGCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTGATTGAAGCGAGCCGCTCa                                                                                                                                                                                                                <  2:126078/250‑1 (MQ=255)
                                                  aTGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACACCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACCCTTTTTAGCag                                                                                                                                                                                             >  2:82403/1‑249 (MQ=255)
                                                     gcaggcACTGCGCGGTTCCAGAACAATGTAGCCGCAGAGCAAAACGACGGGAGCAATGTGGTTGTTCAGATCGCGACGACTCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGCTGTAACTTATCGCTGCGTTTTAATTCTTGCAGCCGCTGCCCGATACGCTCTTCGCGACGTGTTTACCTAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTAAATACTACACTTTTTAGCag                                                                                                                                                                                             >  2:556212/4‑246 (MQ=255)
                                                       tggcACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCATATCGCGACGAATCGACATCTCCGAAACCCCAAGCATGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATc                                                                                                                                                                                        >  2:361497/1‑249 (MQ=255)
                                                             tGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTACCTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGTAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGc                                                                                                                                                                                >  1:21892/1‑251 (MQ=255)
                                                              gcgcGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAATCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACg                                                                                                                                                                                 <  1:22766/249‑1 (MQ=255)
                                                                   gTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCAccc                                                                                                                                                                            >  2:435488/1‑249 (MQ=255)
                                                                       cAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCtt                                                                                                                                                                      <  1:447386/251‑1 (MQ=255)
                                                                           aCAATATAGCCGCCGAGCAAAACGACAGCCGCACAGTGATTGCTAAAATCGCGCCGACTCGAAATCTCCGCAACCCCCCGCAGGGCGGCGCCGTCTTAACGCGGTAACTAATCGCTGCGTTCTACTTCTTCCAGCAGCTGCCCGCAACGCCCTTCGCGACGTCTTTACATAATACCTTTGCATAGTTATTGAAGCGCGCCGCCCAATACTACACTTTTTAGCAGAGATCAGTCACGCCCCCAGCCTTTGCg                                                                                                                                                                  >  2:298747/1‑251 (MQ=255)
                                                                                agaGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAGCCCCAAGCAGGGCGGCGGCGTCTGTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTAGTGAAGCGAGCCGCGCAATTCTACACTTTTTAGCAGAGATCAGTCACGCTCCCAGCCTTTGCGGATc                                                                                                                                                              <  2:537632/248‑1 (MQ=255)
                                                                                       ccgAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGc                                                                                                                                                      >  1:269573/1‑251 (MQ=255)
                                                                                           gCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCaaaa                                                                                                                                                  <  2:245602/251‑1 (MQ=255)
                                                                                            cAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAAc                                                                                                                                                 >  2:197698/1‑251 (MQ=255)
                                                                                                     gggCGCACTGTGGTTGTTCAGCTCGCGACGAATCGTCATCTCCGAAACCACAAGCAGGGCGGCGGCGTATTTAAGCTGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAACACAGACGCGAt                                                                                                                                        >  2:318722/1‑251 (MQ=255)
                                                                                                       gcgcACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTCACTTATCGCTGCGTTTTAATTCTTGCCGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACGCTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATAc                                                                                                                                      >  2:379048/1‑251 (MQ=255)
                                                                                                              gtgGGTGTTCAGATCGCGACGAATCGTCTTCTCCTAATCCCCCAGCAGGGCTGCTGCGTCTTTCAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATTCGCTCTGCGCGACGTGTTTACATATTCCCTCTGAATAGTTACTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATAAGTCACTCCCCCAGCCTTTGCGGATCGGTACTGCAAAACATCCGCTATACCTCGAt                                                                                                                                <  2:165464/250‑1 (MQ=255)
                                                                                                                ggTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGc                                                                                                                             >  2:140103/1‑251 (MQ=255)
                                                                                                                    gttCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACGTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAAGCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGAc                                                                                                                         <  2:346741/251‑1 (MQ=255)
                                                                                                                            cgcgACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGAAACGCTCTTCGCGACGTGTTTACATAATCCCTCTGATTAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGAAACCACGATTGCAGaagatcgg                                                                                                                   >  1:299797/1‑242 (MQ=255)
                                                                                                                            cccgacgaATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGa                                                                                                                          <  2:299797/240‑1 (MQ=255)
                                                                                                                                 cgaATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGttt                                                                                                            >  2:483181/1‑251 (MQ=255)
                                                                                                                                  gaATCGTCATCGCCGAAGCCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCGGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTGTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTa                                                                                                           <  2:208096/251‑1 (MQ=255)
                                                                                                                                    aTCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTAGCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGTATCGGTAATGCAAAACAGACGCTATACCACGATTGCAGACGTTGATAGAGTTTATg                                                                                                         <  1:123413/251‑1 (MQ=255)
                                                                                                                                        tCATCTCCGAAACCCCACGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGcccc                                                                                                     >  2:457885/1‑251 (MQ=255)
                                                                                                                                              ccGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCg                                                                                                 <  1:611812/249‑1 (MQ=255)
                                                                                                                                                gAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGa                                                                                                                                 <  2:272040/215‑1 (MQ=255)
                                                                                                                                                gAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGa                                                                                                                                 >  1:272040/1‑215 (MQ=255)
                                                                                                                                                                  gcggcgTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCTCATCGCTTGCCAGATAATCTGGGCACt                                                                           <  1:303069/251‑1 (MQ=255)
                                                                                                                                                                           ttAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCa                                                                     <  1:233090/248‑1 (MQ=255)
                                                                                                                                                                               gATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATCATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCTCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATc                                                              >  1:267340/1‑251 (MQ=255)
                                                                                                                                                                                                cGTTTTAATTCTTGCAGCAGCTGCCCGATCCGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATcc                                             <  1:457885/251‑1 (MQ=255)
                                                                                                                                                                                                cGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATcc                                             >  1:282851/1‑251 (MQ=255)
                                                                                                                                                                                                    ttAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCg                                         >  1:110845/1‑251 (MQ=255)
                                                                                                                                                                                                     tAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCg                                         >  1:207061/1‑250 (MQ=255)
                                                                                                                                                                                                             gcagcaCCTGCCCGATACGCTCTTCGCGACGTGATTACATAATCCCTCTGAATAGTTATTGAAGCGAGACGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGATGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGCTAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACAGAGCATATcc                                >  1:503307/1‑251 (MQ=255)
                                                                                                                                                                                                              cagcagCTTCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCa                               <  2:410238/251‑1 (MQ=255)
                                                                                                                                                                                                                gcagcTGCCCGATACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATACTCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGc                             >  1:404783/1‑251 (MQ=255)
                                                                                                                                                                                                                           aTACGCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGAc                  <  1:197698/251‑1 (MQ=255)
                                                                                                                                                                                                                               gCTCTTCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGcc               >  2:497411/1‑250 (MQ=255)
                                                                                                                                                                                                                                  ctTCGCGACGTGTTTACATAATCCCGCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAATGCAGCCAGTCATTCCTGCCAGCAGTCCACCGAGCATATCCAGCGGCCAGTGGACGCCaa             <  1:75134/249‑1 (MQ=255)
                                                                                                                                                                                                                                    tCGCGACGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAg            <  1:142452/248‑1 (MQ=255)
                                                                                                                                                                                                                                          cGTGTTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAAcgcg   <  2:65528/251‑1 (MQ=255)
                                                                                                                                                                                                                                           gtgtTTACATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGTATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAAcgcgc  <  1:82403/251‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    
GCCCGGCGTTTTTCTTCCACCAGGCGGGATTTTTGATCGCTTAACAGGTAATGGCTGGCACTGCGCGGTTCCAGAACAATATAGCCGCCGAGCAAAACGACGGGCGCACTGTGGTTGTTCAGATCGCGACGAATCGTCATCTCCGAAACCCCAAGCAGGGCGGCGGCGTCTTTAAGATGTAACTTATCGCTGCGTTTTAATTCTTGCAGCAGCTGCCCGATACGCTCTTCGCGACGTGTTTCCATAATCCCTCTGAATAGTTATTGAAGCGAGCCGCTCAATACTACACTTTTTAGCAGAGATCAGTCACGCACCCAGCCTTTGCGGATCGGTAATGCAAAACAGACGCGATACCACGATTGCAGACGTTGATAGAGTTTATGCCCCATCGCTTGCCAGATAATCTGGGCACTAAGGCAGCCAATCATACCTGCCAGCAATCCACCGAGCATATCCAGCGGCCAGTGGACGCCAAGATAAACGCGC  >  NC_000913/882490‑882975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑