Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	708,774	G→A	A147A (GCG→GCA)	chiP →	chitooligosaccharide outer membrane channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	708,774	0	G	A	100.0%	146.9 / NA	47	A147A (GCG→GCA)	chiP	chitooligosaccharide outer membrane channel
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/26);  total (21/26)

CCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCG  >  NC_000913/708527‑709011                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              ccaaccTTTCTCACTCCACCTGGAATGCCAACCGCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAgg                                                                                                                                                                                                                                            <  1:563270/251‑1 (MQ=255) acaaccTTTCTAACTCAAGCTGGAATGCGAACTTCGATTTTCAGTCCGGTCCTGCTGCTGCTATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGCTATCGCGTTTTCCAAAAGTAATATAGCCTATGACGAAGGCTGGTCCGGCGTCATAAGGCGTATATGCCTGTGTAGAGCTGCGGCCAACCTTAAATTCGGTACGGTTTGGGCAAgg                                                                                                                                                                                                                                            >  2:76077/2‑251 (MQ=255)  caaccTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAggg                                                                                                                                                                                                                                           >  1:67265/1‑251 (MQ=255)     ccTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGc                                                                                                                                                                                                                                          >  1:428491/1‑249 (MQ=255)            cactactccTGGAATTCCAACCTCGATTTTCAGTCCGGGTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATAGCGGAAAACGTCGACAGCCCCCACCCGAACGAAATCGCGTTTGCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGTCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTAc                                                                                                                                                                                                                                   <  2:577090/242‑1 (MQ=255)                  aCCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGcc                                                                                                                                                                                                                           <  2:14326/251‑1 (MQ=255)                    cTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCa                                                                                                                                                                                                                          >  2:154041/1‑250 (MQ=255)                               aCCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGCCAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGGAATAAAGCCTATGACGAAGACTGGTCCGGCGAAAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACg                                                                                                                                                                                                              >  1:530932/1‑251 (MQ=255)                                     aTTTTCGGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTa                                                                                                                                                                                                        <  2:428491/251‑1 (MQ=255)                                                ggTTATGCTGCTGATATTTTCGGCCTTGATATTGCTGCGGTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGTGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTg                                                                                                                                                                                             <  2:27248/251‑1 (MQ=255)                                                   tATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTgg                                                                                                                                                                                            >  1:42705/1‑249 (MQ=255)                                                         gctgATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTa                                                                                                                                                                                     <  1:154041/250‑1 (MQ=255)                                                         gctgATATGTTCGGCATTTATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCGGTATAAAGCGGCGGCCAAATTTAAATACGCACCGGTTTGGGCAAGTGCAGGTTACATTCAGCCAACTGGGCAAACGTTGTGATCGCCGCACTTGTGCttt                                                                                                                                                                                      <  2:95087/249‑1 (MQ=255)                                                           tgATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGACCGAAATCGCGTTTTCAAAAAGTAATAAAGCCTCTGACGAAGCCTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTCTGc                                                                                                                                                                                  >  1:249132/1‑251 (MQ=255)                                                               atGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAg                                                                                                                                                                               <  2:88073/250‑1 (MQ=255)                                                                  ttCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGGAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACCAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGACAACTGGACAAACGCATTTAGCGCCGGCCTGGAGCtgttgaccaggtac                                                                                                                                                                           >  2:17133/1‑238 (MQ=255)                                                                           gATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCACCTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGGACTTCTCAggg                                                                                                                                                                  >  2:335419/1‑251 (MQ=255)                                                                             tatTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAggggg                                                                                                                                                                >  2:351393/1‑251 (MQ=255)                                                                             tatTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAggggg                                                                                                                                                                >  1:620698/1‑251 (MQ=255)                                                                                 gctgcGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCgg                                                                                                                                                             <  1:293990/250‑1 (MQ=255)                                                                                         tACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCgggg                                                                                                                                                    >  1:169308/1‑251 (MQ=255)                                                                                                          cGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGc                                                                                                                                   <  1:402208/251‑1 (MQ=255)                                                                                                                 cGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTg                                                                                                                            <  1:337217/251‑1 (MQ=255)                                                                                                                  ggCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCACATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGACAAACGCTGTTAGCGCCGCACTGGCGCTTTATGCCAGGTACTTCTCAGGGGGCGGAAGCCGGG‑CGAATTTTGATTACGGCGATGCTggg                                                                                                                          >  1:106543/1‑250 (MQ=255)                                                                                                                          cTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTg                                                                                                                    >  1:374392/1‑250 (MQ=255)                                                                                                                            cccACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGt                                                                                                                 <  2:182300/251‑1 (MQ=255)                                                                                                                                      cgaaATCGCGTTTTCAAAAAGTACTAAAGCCTATGACGACGACTGGTCCGGCGACCAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCCCCTAc                                                                                                        >  2:366548/1‑250 (MQ=255)                                                                                                                                       gaaATCACGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGCTTACGGCGATGCGGGTGCGTTGAGTTTCTCCTACAt                                                                                                      >  1:489607/1‑251 (MQ=255)                                                                                                                                        aactCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACAtg                                                                                                     <  1:522872/248‑1 (MQ=255)                                                                                                                                          aTCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACAtgtg                                                                                                   <  1:150712/251‑1 (MQ=255)                                                                                                                                            cgcgTTTTCAAAAAGTAATAAAGCCTATGACGAAGTCTGGTCCGGCGACAAAAGCTGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTGCATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGTTACTTATCAGGGGGCGGGAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGa                                                                                                 <  1:76077/251‑1 (MQ=255)                                                                                                                                               gTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCa                                                                                              <  1:335419/251‑1 (MQ=255)                                                                                                                                                   tCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACg                                                                                           >  2:169712/1‑250 (MQ=255)                                                                                                                                                             ataaAGCCTATGACGAAGACGGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAgcg                                                                                <  2:90762/251‑1 (MQ=255)                                                                                                                                                                      aTGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCAt                                                                       <  1:344533/251‑1 (MQ=255)                                                                                                                                                                                        gCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTTGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGttt                                                     <  1:451457/251‑1 (MQ=255)                                                                                                                                                                                           aCAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTAt                                                  >  1:34455/1‑251 (MQ=255)                                                                                                                                                                                               aaGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATc                                                 >  2:348179/1‑248 (MQ=255)                                                                                                                                                                                                        aaGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTTGTGCGTTGAGTTGCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGAGGAGTGTTATCATAACGATaaa                                      <  2:587866/250‑1 (MQ=255)                                                                                                                                                                                                        aaGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATaaaa                                     <  2:528945/251‑1 (MQ=255)                                                                                                                                                                                                        aaGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGCCCGATTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGAGGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGGTTTATCAGAACGATaaaa                                     <  1:233916/251‑1 (MQ=255)                                                                                                                                                                                                        aaGCCTGTATAAAGCTGCGGCCAAACTTAAATTCGGTCCTGTTTGAGCAATGTCACGTTACATTCAGCCAACTTGTCAAACGCTGTTAGCGCCTCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGTTTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGTCCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATaaaa                                     <  1:105010/251‑1 (MQ=255)                                                                                                                                                                                                         aGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAac                                    <  1:36167/251‑1 (MQ=255)                                                                                                                                                                                                              gTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAaccacc                                >  1:435874/1‑250 (MQ=255)                                                                                                                                                                                                                                aTTTAAATACGGTCCGGTTTGGGCAAGGGCAGGTTACATGCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACGTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGAGGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCTCCTTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACt             <  2:320222/251‑1 (MQ=255)                                                                                                                                                                                                                                    aaaTACGGTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCtt         >  1:497171/1‑251 (MQ=255)                                                                                                                                                                                                                                           gTCCGGTTTGGGCAAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCg  <  2:348880/251‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                              CCAACCTTTCTCACTCCACCTGGAATGCCAACCTCGATTTTCAGTCCGGTTATGCTGCTGATATGTTCGGCCTTGATATTGCTGCGTTTACGGCGATTGAAATGGCGGAAAACGGCGACAGCTCCCACCCGAACGAAATCGCGTTTTCAAAAAGTAATAAAGCCTATGACGAAGACTGGTCCGGCGACAAAAGCGGTATAAGCCTGTATAAAGCTGCGGCCAAATTTAAATACGGTCCGGTTTGGGCGAGGGCAGGTTACATTCAGCCAACTGGTCAAACGCTGTTAGCGCCGCACTGGAGCTTTATGCCAGGTACTTATCAGGGGGCGGAAGCCGGGGCGAATTTTGATTACGGCGATGCTGGTGCGTTGAGTTTCTCCTACATGTGGACCAACGAATACAAAGCGCCGTGGCATCTGGAAATGGATGAGTTTTATCAGAACGATAAAACCACCAAAGTTGATTATCTGCACTCCTTTGGGGCG  >  NC_000913/708527‑709011

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑