Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	694,941	G→A	L5L (CTC→CTT)	miaB ←	isopentenyl‑adenosine A37 tRNA methylthiolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	694,941	0	G	A	100.0%	173.4 / NA	56	L5L (CTC→CTT)	miaB	isopentenyl‑adenosine A37 tRNA methylthiolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/27);  total (29/27)

CTTGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGA  >  NC_000913/694694‑695184                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    cttGCGCTGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGCTGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGCCAGCCCCAGGTTTTAATATGAAGt                                                                                                                                                                                                                                                  >  2:32061/1‑251 (MQ=255)          ccACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTc                                                                                                                                                                                                                                         <  1:383824/251‑1 (MQ=255)                 gCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGGCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACt                                                                                                                                                                                                                                  <  1:311002/251‑1 (MQ=255)                  ccACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACtt                                                                                                                                                                                                                                 <  1:179041/251‑1 (MQ=255)                         cGCCGATAAACAGGTCTGGATTCTGCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTGCCTGAGCCTTCGCGCGGATTGAGCAGGGGTTCAGCAGCAGCACATCCGCTCATTCCGCCACGTCGGTCAGTTGATATCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTGCTCGTTCATCTGACAGCCCGAGGTTTTAATATGAAGGTTTTTGGTCATCGACTTGCTCTTg                                                                                                                                                                                                                          <  2:253109/251‑1 (MQ=255)                            cGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGAAGGAGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGCTGAATCGTACTCGTTCATCTGCCAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCg                                                                                                                                                                                                                        >  2:499310/1‑250 (MQ=255)                                      gTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGGGGTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACGTGCTCTTGCGAAATAGTAGcc                                                                                                                                                                                                             <  1:204338/251‑1 (MQ=255)                                               cttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGc                                                                                                                                                                                                     >  1:205108/1‑250 (MQ=255)                                               cttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGGGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGc                                                                                                                                                                                                     >  1:438027/1‑250 (MQ=255)                                                ttctCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGc                                                                                                                                                                                                     >  2:494467/1‑249 (MQ=255)                                                 tctcTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGCCAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAgg                                                                                                                                                                                                  >  2:617422/1‑251 (MQ=255)                                                         aaCAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGATTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGAGCGCAt                                                                                                                                                                                            >  2:22391/1‑249 (MQ=255)                                                          aCAGTTTCCAGCGACACAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGCTCGGCCATCTTCGATGAATCGTACTCGTTCATCTGCCAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCCTAgt                                                                                                                                                                                         >  2:461758/1‑251 (MQ=255)                                                                  cAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCATGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTTCTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCGTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCt                                                                                                                                                                                 <  1:132722/251‑1 (MQ=255)                                                                          cAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCCGGCGTTCAGCAGCAGCACATCAGCTTCTTCAGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGACCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGcc                                                                                                                                                                         >  1:562210/1‑251 (MQ=255)                                                                               gATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTtgt                                                                                                                                                                    >  2:535634/1‑251 (MQ=255)                                                                                          ttttCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATg                                                                                                                                                          <  1:431757/250‑1 (MQ=255)                                                                                            ttCCTGAGCCTTCTCGCGGATTGATCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTTGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCTTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGTTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGTGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAg                                                                                                                                                        <  1:535389/250‑1 (MQ=255)                                                                                                  aGCCTTCTCGCGGCTTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGttttt                                                                                                                                                                                                                                              >  1:483004/1‑158 (MQ=255)                                                                                                  aGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGttttt                                                                                                                                                                                                                                              <  2:483004/158‑1 (MQ=255)                                                                                                     cTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAg                                                                                                                                                                                                   >  1:434796/1‑198 (MQ=255)                                                                                                     cTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAg                                                                                                                                                                                                   <  2:434796/198‑1 (MQ=255)                                                                                                           gcgGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCTGTCAGTTGATAGCCATGGGTGGCATCCATCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCATGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGGAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAg                                                                                                                                        <  1:306256/251‑1 (MQ=255)                                                                                                              gATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAgggg                                                                                                                                     <  2:398268/251‑1 (MQ=255)                                                                                                               aTTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGt                                                                                                                                    >  1:331839/1‑251 (MQ=255)                                                                                                                 tGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTa                                                                                                                                   <  1:595441/250‑1 (MQ=255)                                                                                                                      aGGTGTTCAGCAGCAGCCCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGGTTTAATATGAAGGTTTTTGGTCATCGACTTGCTCTTGCGAAATATTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCTTTGTGACCAGTATGATCGTTATCAGCCCTTAGGGGTAAAAATc                                                                                                                             <  1:582457/251‑1 (MQ=255)                                                                                                                      aGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATc                                                                                                                             <  1:229091/251‑1 (MQ=255)                                                                                                                          gtTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGGTCATCTGACAGCCCCCGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGCAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGt                                                                                                                         >  1:184891/1‑251 (MQ=255)                                                                                                                            tCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTa                                                                                                                        <  1:227623/250‑1 (MQ=255)                                                                                                                             cagcagcagCCAATCCGCTTCGTCCGCCTCGGCGGTCAGTTGAGAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCTTACTCGGTCATCTGACAGCCCCAGGTTGTAATATGAAGTTTTTTGGTCATCGACTTGCGTTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTAGCAGCCCTTAGGGGTAAAAATCCTGTaaa                                                                                                                      <  2:514678/251‑1 (MQ=255)                                                                                                                                cagcagcaCATCCGCTTCTTCCGCCACGTCGGTCAGGTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGGTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACtt                                                                                                                   <  1:593007/251‑1 (MQ=255)                                                                                                                                    agcaCATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTaa                                                                                                                 >  1:545142/1‑249 (MQ=255)                                                                                                                                      cacaTCCGCTTCTTCCGCCCCGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTaaatca                                                                                                             <  2:130498/251‑4 (MQ=255)                                                                                                                                        caTCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCata                                                                                                           <  1:32061/251‑1 (MQ=255)                                                                                                                                                tcttcCGCCACGTCGGTCAGTTGATAGCCGGGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACGTAAAGCATATTGCTa                                                                                                     <  2:72103/249‑1 (MQ=255)                                                                                                                                                           gTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTg                                                                                         >  2:176158/1‑250 (MQ=255)                                                                                                                                                             cGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGAc                                                                                       <  1:218343/250‑1 (MQ=255)                                                                                                                                                                      gATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATTACAAAt                                                                             <  1:422042/251‑1 (MQ=255)                                                                                                                                                                         aGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTGGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATca                                                                           <  2:519064/250‑1 (MQ=255)                                                                                                                                                                                  tggCATCCAGCAGATCGGCCATCTTCGATGAATCGCACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGTGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGa                                                                  >  1:177248/1‑250 (MQ=255)                                                                                                                                                                                             agATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGt                                                      >  1:162434/1‑251 (MQ=255)                                                                                                                                                                                              gATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTc                                                     <  1:612509/251‑1 (MQ=255)                                                                                                                                                                                                 cGGCCATCTTCGATGAATCGTCCTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTcggc                                                  >  1:486897/1‑251 (MQ=255)                                                                                                                                                                                                    ccATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGAGCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCgga                                               <  2:107091/251‑1 (MQ=255)                                                                                                                                                                                                       tCTTCGATGACTCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCggagg                                             >  1:151329/1‑250 (MQ=255)                                                                                                                                                                                                         ttCGATGACTCGTACTCGCTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCCGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAAt                                          >  2:239166/1‑251 (MQ=255)                                                                                                                                                                                                            gATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCCTCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGGAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCATGTACACTTAAAGCATATTGCTAACAGGATGATTGACCATGACACATCAACAAACGGAAATTGCCATTGTCGGCGGAGGCATggg                                       >  2:8094/1‑250 (MQ=255)                                                                                                                                                                                                            gATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGACATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGt                                       >  2:228321/1‑251 (MQ=255)                                                                                                                                                                                                                    gTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGgcggcg                               >  1:17631/1‑251 (MQ=255)                                                                                                                                                                                                                               tCTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTg                    >  1:243991/1‑251 (MQ=255)                                                                                                                                                                                                                                cTGACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGAGCTgg                   >  1:377471/1‑251 (MQ=255)                                                                                                                                                                                                                                  gACAGCCCCAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTgggg                 >  1:119788/1‑251 (MQ=255)                                                                                                                                                                                                                                          cAGGTTTTAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGACGGCGGCGCTCTGGCGCTGGGGCTGGcaca         <  2:59132/251‑1 (MQ=255)                                                                                                                                                                                                                                               tttAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACg    >  1:346310/1‑251 (MQ=255)                                                                                                                                                                                                                                                 tAATATGAAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCCCAGCACggg  >  2:246126/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                    CTTGCGATGCCACGCAGCCACCGACGCCGATAATCAGGTCTGGATTCTTCTCTTTTAACAGTTTCCAGCGACCCAACTGATGGAAGACTTTTTCCTGAGCCTTCTCGCGGATTGAGCAGGTGTTCAGCAGCAGCACATCCGCTTCTTCCGCCACGTCGGTCAGTTGATAGCCGTGGGTGGCATCCAGCAGATCGGCCATCTTCGATGAATCGTACTCGTTCATCTGACAGCCCCAGGTTTTAATATGGAGTTTTTTGGTCATCGACTTGCTCTTGCGAAATAGTAGCCAGGAATGCAGGGCGCATAGTGTAATGCTTTGCTGCCGTTGTGACCAGTATGAGCGTTATCAGCCCTTAGGGGTAAAAATCCTGTAAACTTAAAGCATATTGCTAACAGGATGATTGACCATGACAAATCAACCAACGGAAATTGCCATTGTCGGCGGAGGAATGGTCGGCGGCGCACTGGCGCTGGGGCTGGCACAGCACGGA  >  NC_000913/694694‑695184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑