Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	677,829	T→C	W139R (TGG→CGG)	djlB →	J domain‑containing protein DjlB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	677,829	0	T	C	100.0%	68.1 / NA	25	W139R (TGG→CGG)	djlB	J domain‑containing protein DjlB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (1/24);  total (1/24)

GCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACTGCA  >  NC_000913/677583‑678070                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  gCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTTGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTTCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTTTTATTAGCAGAAAGATTGCGGCGGtt                                                                                                                                                                                                                                               <  2:538883/251‑1 (MQ=255)                cAACCAGCAGAATATGATGTAGCAGAACATGAAATTCTGCTCGCCTTGCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACAATGCCCGTTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTCGGGCGGTTTCAGGAGGAAAATAc                                                                                                                                                                                                                                <  2:381505/251‑1 (MQ=255)                     agaagaATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAATATTGCGTCGGTTACAGGAGGAAAATACCgggg                                                                                                                                                                                                                           <  2:379852/251‑1 (MQ=255)                        agaaTATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAgggggagaaaaacagggga                                                                                                                                                                                                                         >  1:618306/1‑237 (MQ=255)                           atatGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCAGTTAGCCTTCGAGTGCGTGGTATTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATGCCGGGGAAATAg                                                                                                                                                                                                                     <  2:283623/251‑1 (MQ=255)                             atGAGTTAGCAGAACAGGAAATTCTGCTCGACTTTCGTGCGTTACTTGCCTCTGATAGTTAACGTTTTCGGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAAGTATTGCTCGATGGAGGAGATTGATGAATTACGCTTGTCTCTGTGCACAATCGCTACGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTAGTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAAtcga                                                                                                                                                                                                                    <  2:308711/250‑4 (MQ=255)                                           cATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGGTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGa                                                                                                                                                                                                     <  1:498178/251‑1 (MQ=255)                                                          gCCTTTCGTGCGTTACTTTCCTCTGATAGTGACCTTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCATCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTTCGCTGGTCGCTGTGCTCAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTTGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAGTCCTTTTTATATGc                                                                                                                                                                                      <  2:277072/251‑1 (MQ=255)                                                              ttCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCAt                                                                                                                                                                                   <  2:193769/250‑1 (MQ=255)                                                                                                                   cGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGTCCATTTTGCATCTGCCCGTTGCCGt                                                                                                                             <  2:430493/251‑1 (MQ=255)                                                                                                                     attcattcAGCAATTTAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGtt                                                                                                                            <  2:354587/250‑2 (MQ=255)                                                                                                                              gCAATTAAATTATTGCTCGATGGAGGAGATTGATGAAGTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTATTAGTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGaca                                                                                                                  <  2:371811/251‑1 (MQ=255)                                                                                                                                               cGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTAcc                                                                                                   <  1:121251/249‑1 (MQ=255)                                                                                                                                                             gatgaATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTATTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAATAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGGGCAAAATGACACCATCGATTTTTACCAAATGTTAGCTCGGa                                                                                    <  2:319256/250‑1 (MQ=255)                                                                                                                                                                             cGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCca                                                                   <  1:9236/251‑1 (MQ=255)                                                                                                                                                                                   gCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTATTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCCCAATgg                                                             <  2:543451/251‑1 (MQ=255)                                                                                                                                                                                        aTCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTGTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGAc                                                        <  1:414811/251‑1 (MQ=255)                                                                                                                                                                                          cGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACAt                                                      <  2:43963/251‑1 (MQ=255)                                                                                                                                                                                                gAACACTGCCCATTTATCCTTCGAGTGCGGGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACTCCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTAt                                                 <  2:506756/250‑1 (MQ=255)                                                                                                                                                                                                  acacTGCACAGTTATCCTGCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACCCCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTCACATTGTATTTa                                              <  2:394896/251‑1 (MQ=255)                                                                                                                                                                                                                     cGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGa                           <  1:183108/251‑1 (MQ=255)                                                                                                                                                                                                                           cGTGGTGTTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATcc                     <  1:519549/251‑1 (MQ=255)                                                                                                                                                                                                                               gtgtTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCga                 <  1:542047/251‑1 (MQ=255)                                                                                                                                                                                                                                 gtTATTAGCAGAAAGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCgat                <  1:135408/250‑1 (MQ=255)                                                                                                                                                                                                                                              aGATTGCGGCGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACTGca  <  1:150685/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                  GCTAAATCTGTTTGGCAACCAGAAGAATATGAGGTAGCAGAACATGAAATTCTGCTCGCCTTTCGTGCGTTACTTGCCTCTGATAGTGAACGTTTTCTGCCCTCCGCCTGGCAGCGATTCATTCAGCAATTAAATTATTGCTCGATGGAGGAGATTGATGAATTACGCTGGTCGCTGTGCACAATCGCCATGAACACTGCCCATTTATCCTTCGAGTGCGTGGTGTTATTAGCAGAAAGATTGCGGTGGTTACAGGAGGAAAATACCGGGGAAATAGACGAAGAAGAACTGGAATCCTTTTTATATGCCATTGCGAAGGGGAATGTTTTTAACTTCCAGACCATTCTGCATCTGCCCGTTGCCGTGCAAAATGACACCATCGATTTTTACCAAATGTTCGCTCGGATTTGGTCATCGCATCCACAATGGCTGACATTGTATTTAGCGCAACATCGCGCAGTGATTATCCCCGATGATGCAAAACTGCA  >  NC_000913/677583‑678070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑