| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 533,866 | C→T | intergenic (+3/‑50) | allZ → / → gcl | small RNA AllZ/glyoxylate carboligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 533,866 | 0 | C | T | 93.1% | 72.7 / ‑1.3 | 29 | intergenic (+3/‑50) | allZ/gcl | small RNA AllZ/glyoxylate carboligase |
| Reads supporting (aligned to +/- strand): ref base C (0/2); new base T (18/9); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.31e-01 | |||||||||||
TATACCGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCAC > NC_000913/533620‑534083 | tataCCGTAGATAAAGAAGAGCATGATGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGACGGTAGTGATGTTGCCGCTATCTCCATCTCCGGGACTTCATCAAGACTGACAGAAGATCGTTTTGGCAGACAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTAGGGACTGAAAGCACATCCATAATGTCTGTCGCATCACGCTATGCGGAGCGGGGTTTTTTGATaaa > 2:128139/1‑250 (MQ=255) cGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGa < 1:484518/251‑1 (MQ=255) cATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTAGCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGGCAGTCAGGGTGAGCTGGGCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGTAGCGGGGTTTATTGATAAAATTTGAAAGTTGGAAAAAGGGTCCAAGAAAGAGAGGTAGGaa < 2:409658/251‑1 (MQ=255) tttACGCTTATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATCAATAGAGGTAGGAATAAAATGGCaaaa > 2:72136/1‑250 (MQ=255) aCGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATg < 2:339309/250‑1 (MQ=255) gatgatGTCGGTAGTGTTGTTGCCGCTATCTCCATCGCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATgaga < 2:475407/251‑1 (MQ=255) cGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTg > 2:540514/1‑250 (MQ=255) gtgtTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCgg < 1:270725/251‑1 (MQ=255) gttgttGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGc > 1:253148/1‑250 (MQ=255) ccGCTCTCTCCCTCTCCGGACCTTCATCAAGACTGCTAGAAGATCGTTTTGTCAGTCAGGGTGCGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCGatgtatg > 2:365736/1‑251 (MQ=255) tccGGGCCTTCCTCAAGACTGACAGTAGCTCGTTTTGTCCGTCAGGGCGAGCTGGTCAGAGACACCGCCCGCGATCTCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGagaaaga > 2:368548/1‑250 (MQ=255) gCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTAt > 2:23551/1‑251 (MQ=255) gaagaTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGtt > 1:217133/1‑251 (MQ=255) aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCgg > 2:572669/1‑251 (MQ=255) aTCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGAACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGAGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAGATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGGATCACTACCGCCTTCGGGGTTCCgg > 1:29687/1‑251 (MQ=255) tGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGc > 1:538080/1‑251 (MQ=255) tcagGGTGAGCTGGCCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGCGCGGGTTTTTTTGATAAAATTTGAAAGTTGGACAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGGGTTCCGGGAGCTGcaatca > 2:237805/1‑250 (MQ=255) gggcAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACAGCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGACTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTAc < 2:451190/246‑1 (MQ=255) agagACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGATTTTTTGGTAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCTGTTCTACTCAGCg > 1:250617/1‑251 (MQ=255) acCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAACTAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACACGGCAATGTATGTGCTGAAGAACGAAGATATCACTACCGCCTTCGGTGTTCCGGGCGCTGCAATCAATCCGTTCTACTCAGCGATGCGt > 1:619275/1‑251 (MQ=255) atCAGCCCGGCGTTTGGCCTGAAAGCACATCCATAATGACTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAACAATTTTCCAATAAATAGAGGTAGGCATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGACGGCATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGCTCTACTCAGCGATGCGTAAGCAcggcggg > 2:84215/1‑250 (MQ=255) gCACGGCGTTGGGACTGAAAGCACATCCATAATGGCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTc < 1:368548/251‑1 (MQ=255) tGGGACTGAAAGCACATCCATAATGTCGGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTTAAAGTTGGAAAAATTTTCCAATAAATTGAGGGAGGAATAAAATGGCAAAAATGAGCGCGGTTGACTCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAAGCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACAtt < 1:626737/250‑1 (MQ=255) ggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTg < 1:615902/251‑1 (MQ=255) ggACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTAAGCGATGCGTAAGCACGGCGGGATTCGTCCCATTCTg > 2:413081/1‑251 (MQ=255) aaGCACATCCATGATGTCTGTCGCTGCCAGCTGATCGATGCGGGATTCTTTAATAAAATTTACACGTTGGAAAAATTTTCCAGTAAATAGCGGTAGAAATAAAATGGCGAGAATGAGAGCCGTTGAAGCGGCAATGAGTGTGCTGGAGAAAGCAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCCATCACTCCGTTCTACTCTGCGATGAGTAAGCAAGGCGGGATTCGCCACATTTTGGCGCGTc > 2:461014/1‑251 (MQ=255) cacaTCCATAATGTGTTTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGATAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCTTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACAGGCTGGCGCGTcttg < 2:410802/251‑4 (MQ=255) aaTGTCTGTCGCATCCCGCTCTGCAGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATTAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGt < 1:217181/250‑1 (MQ=255) cGCATCCCGCTCTGCGGAGCGGGTTTTTTTGATAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGcac > 1:567205/1‑250 (MQ=255) | TATACCGTAGATAAAGAAGAGCATGTTGTAGGTCTGAATTGCATAGCTTCAGCAATTTACGATGATGTCGGTAGTGTTGTTGCCGCTATCTCCATCTCCGGGCCTTCATCAAGACTGACAGAAGATCGTTTTGTCAGTCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTGGGACTGAAAGCACATCCATAATGTCTGTCGCATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAAAGTTGGAAAAATTTTCCAATAAATAGAGGTAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACTACCGCCTTCGGTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCATGTGGAAGGTGCTTCGCAC > NC_000913/533620‑534083 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |