Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	491,939	C→T	S176S (AGC→AGT)	apt →	adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	491,939	0	C	T	97.9%	278.6 / NA	96	S176S (AGC→AGT)	apt	adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (53/40);  minor base G (0/2);  total (54/42)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGC  >  NC_000913/491692‑492177                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                aaaCCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCtt                                                                                                                                                                                                                                              <  1:402753/251‑1 (MQ=255)  aaCCATCAGTGCAACTTACGACCTGGAATAAGGCACCGATAAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGGGGTGAAGTGGCTGACGCTGCGTTCATTATCACCCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTg                                                                                                                                                                                                                                             >  2:387715/1‑251 (MQ=255)   aCCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGt                                                                                                                                                                                                                                            >  2:152017/1‑251 (MQ=255)      aTCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTccc                                                                                                                                                                                                                                         >  1:624203/1‑251 (MQ=255)       tCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCg                                                                                                                                                                                                                                        >  1:298999/1‑251 (MQ=255)              aaCTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGGGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACAAGCTACAGTCTTGTCCCGTTCCC‑gg                                                                                                                                                                                                                                >  1:27469/1‑251 (MQ=255)                cctACGACCTGGAATACGGCACCCATCATCTGGAGAGCCTCGGTGATGCCATCAACCCGGGCGACAAAGTTCTGGTTGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTTAAGTGGCTGACGTTGCGTTCATTATCATCCTGTT‑CGATCTCGGCGGCGAACAGCTTCTCGAAAAACAGGGCATTACCAGCGACAGTCCTGTCCCGTTCCC‑GGGc                                                                                                                                                                                                                              <  1:304354/249‑1 (MQ=255)                  tACGTCCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTTATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTAGCGAAGCGACCGGTAAACTGATTCGGCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCAGTATCAACCTGTT‑CGATCTCTGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGATACAGGCtttccccgggcccgtgc                                                                                                                                                                                                                               <  2:77178/249‑16 (MQ=255)                  tACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTGCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGc                                                                                                                                                                                                                              <  2:121579/249‑1 (MQ=255)                  tACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCACCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGc                                                                                                                                                                                                                              <  1:313360/249‑1 (MQ=255)                           gAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATc                                                                                                                                                                                                                   >  2:400280/1‑251 (MQ=255)                                 ggCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTACTTATCGCCAGt                                                                                                                                                                                                             >  1:119343/1‑251 (MQ=255)                                    aCCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGtatt                                                                                                                                                                                                          >  2:470188/1‑248 (MQ=255)                                          cAGCTGGAGTGCCACGTTGATGCCAGCAAACCTGGGGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGCCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCGCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTGCCGTTCCC‑GGGCCAGTAATTATCGCCAGTCTtgtgat                                                                                                                                                                                                     <  2:214197/250‑3 (MQ=255)                                          cAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGtgctg                                                                                                                                                                                                    >  2:189074/1‑251 (MQ=255)                                                     ccACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTAc                                                                                                                                                                                          <  1:306483/250‑1 (MQ=255)                                                            gATGCCATCAAACAGGGAGACACAGTTCTGGTGGAGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGACTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGc                                                                                                                                                                                   >  1:434031/1‑250 (MQ=255)                                                               gCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAATCTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTAAAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGcaca                                                                                                                                                                                >  1:595343/1‑250 (MQ=255)                                                                ccATCAAACCTGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCGGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAGCAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCGGCCCACGCTACGGACTGCACAAg                                                                                                                                                                              <  2:402950/251‑1 (MQ=255)                                                                  aTCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGcaca                                                                                                                                                                                >  1:415144/1‑247 (MQ=255)                                                                      aaCCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTAATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGAtgggc                                                                                                                                                                        >  2:508861/1‑248 (MQ=255)                                                                          gggCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTc                                                                                                                                                                    <  1:54574/251‑1 (MQ=255)                                                                           ggCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCa                                                                                                                                                                   <  1:189074/251‑1 (MQ=255)                                                                           ggCGACAAAGTGCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTTTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAACACAAGATGTGCATTCa                                                                                                                                                                   <  2:249484/251‑1 (MQ=255)                                                                            gCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAg                                                                                                                                                                  <  2:74695/251‑1 (MQ=255)                                                                            gCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTAGCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAg                                                                                                                                                                  <  2:607129/251‑1 (MQ=255)                                                                             cGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGc                                                                                                                                                                 >  1:458939/1‑251 (MQ=255)                                                                                cAAAGTTTTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAATCAGGGCATTTCCAGCTACAGTCTTGTCCCTTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTCCGGACAGCACAAGATGTGCATTCAGcttc                                                                                                                                                              <  2:532177/251‑4 (MQ=255)                                                                                   aGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGcc                                                                                                                                                           <  1:573308/251‑1 (MQ=255)                                                                                   aGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGGCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGcc                                                                                                                                                           <  1:232839/251‑1 (MQ=255)                                                                                        gggtggtggACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAATCTGATCCGTCGTCTGGTTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCCCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCt                                                                                                                                                      <  2:595343/250‑1 (MQ=255)                                                                                         ggtggtggACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTg                                                                                                                                                     >  2:40021/1‑251 (MQ=255)                                                                                           tggtggACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGAc                                                                                                                                                   >  1:26200/1‑251 (MQ=255)                                                                                            gggggccgacCTGCTGGCAACCGGGTGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAATTGGCTGACGCTGCGTTCATTAGCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCGACGGACAGCACAAGATGTGCATCCAGCCTCGCCGTTCTGACg                                                                                                                                                  <  2:565676/245‑1 (MQ=255)                                                                                             gtggACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAACCTGATCCGTCGTCTGGGTGGTGACGTGGCTGACGCTGCGTTCATTATCACCCTGTT‑CGCTCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACCAGATGTGCATTCAGCCTCGCCGTTCTGACgg                                                                                                                                                 >  1:387280/1‑251 (MQ=255)                                                                                                gacgacCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGc                                                                                                                                              <  1:442545/251‑1 (MQ=255)                                                                                                   gacCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGTGCCGTCGTCTGGGTGGTGAAGAGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCtgt                                                                                                                                           <  1:625039/251‑1 (MQ=255)                                                                                                   gacCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCtgt                                                                                                                                           >  1:206301/1‑251 (MQ=255)                                                                                                       tgctgGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGCTCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGCCGGGGCTGTGtt                                                                                                                                        >  2:272421/1‑250 (MQ=255)                                                                                                        gctgGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCGCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTCCAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGTGGCTGTGTTAg                                                                                                                                      <  2:58980/251‑1 (MQ=255)                                                                                                          tgGCAACCGGCGGCACTATCGAAGCGCCCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGc                                                                                                                                     >  2:554566/1‑250 (MQ=255)                                                                                                          gggCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGGGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCa                                                                                                                                    <  2:435679/250‑1 (MQ=255)                                                                                                           ggCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCAt                                                                                                                                   <  2:206301/251‑1 (MQ=255)                                                                                                           ggCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCAt                                                                                                                                   >  1:244035/1‑251 (MQ=255)                                                                                                                   gcggcACTATCGAAGCGACCTTTAAACTGATCCTTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCtt                                                                                                                           <  2:465212/251‑1 (MQ=255)                                                                                                                    cggcACTATCGAAGCGACCGTTAAACTGATCCGTCGTCGGGGTTGTGAAGTGGCTGACGCTGCGTTCAGTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTGCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCTCGCGACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTc                                                                                                                          <  2:71591/251‑1 (MQ=255)                                                                                                                     ggcACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCg                                                                                                                         >  1:201119/1‑251 (MQ=255)                                                                                                                      gcACTATCGAAGCGACCGTTAAACTGATCCTTCTTCTGTGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CTGTCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGTCCATTAATTATCGCCAGTCTTGTGCCGCCCACGGTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGt                                                                                                                        <  1:590963/251‑1 (MQ=255)                                                                                                                                ggggACCGGTAAACTGAGCCGTCGTCTGGGGGGTGAAGGGGCTGACGCTGCGGTCATTATCAGCCGGGT‑CGGTCTCGTCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCAGTACTCCTTCGTGAATCCACCt                                                                                                              <  2:415144/248‑1 (MQ=255)                                                                                                                                    aCCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCACCCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTGGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCa                                                                                                          <  2:595260/251‑1 (MQ=255)                                                                                                                                    aCCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGGCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCa                                                                                                          <  2:458939/251‑1 (MQ=255)                                                                                                                                             cTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCtt                                                                                                             >  1:78819/1‑240 (MQ=255)                                                                                                                                             cTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCCCGGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCtt                                                                                                             <  2:78819/240‑1 (MQ=255)                                                                                                                                              tGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTc                                                                                                   >  1:322866/1‑248 (MQ=255)                                                                                                                                               gATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCag                                                                                                 >  1:234497/1‑249 (MQ=255)                                                                                                                                                   cgtcgtCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGCCAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCCGCGTTTCAGTGCCTg                                                                                           >  2:107941/1‑251 (MQ=255)                                                                                                                                                       gtcTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCa                                                                                        >  1:289397/1‑250 (MQ=255)                                                                                                                                                         cTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCTCCGTTCTGACGGGGCTGTGTTAGCCTTACCCCTTCGTGAATCAACCTTCCAGCGTTTCAGAGCCTGCCAATg                                                                                     >  2:332486/1‑251 (MQ=255)                                                                                                                                                           gggTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATg                                                                                     >  1:149018/1‑249 (MQ=255)                                                                                                                                                           gggTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGa                                                                                    <  1:532399/250‑1 (MQ=255)                                                                                                                                                               ggtgACGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTAt                                                                               >  1:535183/1‑251 (MQ=255)                                                                                                                                                                  gAAGTGGCTGTCGCTGCGTTCAGTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCTTCTCGAAAAACAGGGCATTACCAGCTGCAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGTTGTGTTAGCATTACCCCTTCGTGAATCCCCCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAg                                                                            <  1:303520/251‑1 (MQ=255)                                                                                                                                                                   aaGTGGCTGACGCTGCGGTCATTATCAACCTGTT‑CGATCTCGGCGGAGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGACCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGATGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTacaagg                                                                           >  1:569183/1‑246 (MQ=255)                                                                                                                                                                       ggCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCtt                                                                       <  2:391855/251‑1 (MQ=255)                                                                                                                                                                       ggCTGACGCTGCGTGCATTAGCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTTTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCtt                                                                       <  1:108947/251‑1 (MQ=255)                                                                                                                                                                       ggCTGACGCTGAGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGACATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGAGCATTCAGCCTCGCCGTTATGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTGATCAgaactt                                                                       >  1:368574/1‑246 (MQ=255)                                                                                                                                                                        gCTGACGCGTCGTTCATTAACAACC‑GTTGCGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCAGTACCAGCTACAGTCTTGGCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTGCAGAGCCTGCCAATGAGTTATCAGGTCTTa                                                                      <  2:27469/251‑1 (MQ=255)                                                                                                                                                                          tGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGc                                                                    >  2:476310/1‑251 (MQ=255)                                                                                                                                                                          tGACGATGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGCACAGTGTCTAGAAAAACAGGGCATTACCCGCTACAGTCTTGTCCCGTTACC‑GGGCCCTTAATTATCGCCAGTCTTGTGCTGCCCCCGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCCTTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGc                                                                    >  1:577429/1‑251 (MQ=255)                                                                                                                                                                           gACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCCCCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGcc                                                                   >  1:40903/1‑251 (MQ=255)                                                                                                                                                                           gACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGcc                                                                   >  1:331819/1‑251 (MQ=255)                                                                                                                                                                           gACGCTGCGTTCATTAGCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGc                                                                    <  2:194004/250‑1 (MQ=255)                                                                                                                                                                             cGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCg                                                                 >  1:522553/1‑251 (MQ=255)                                                                                                                                                                             cGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGGGCATTCACCTTCGCCGTTCTGCCGGGGCTGTGTTAGCATTACCCCTTCGTGAACCCACCTTCCCGCGTTTCGGAGCCTGCCAATGAGTTATCAGGTCTTCGCCCg                                                                 >  2:191529/1‑251 (MQ=255)                                                                                                                                                                              gCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCg                                                                 >  1:564376/1‑250 (MQ=255)                                                                                                                                                                                   gTTCATTATCAACCCGTT‑CGATCTCGGCGACGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCCCAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTCTCAGGTCTTAGCCCGAAAATg                                                           >  2:419666/1‑251 (MQ=255)                                                                                                                                                                                       aTTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGgcgc                                                       >  1:528342/1‑251 (MQ=255)                                                                                                                                                                                           tCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGCATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAACTGGCGCCcac                                                   >  1:545824/1‑251 (MQ=255)                                                                                                                                                                                           tCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTc                                                                                                   >  2:625096/1‑203 (MQ=255)                                                                                                                                                                                             aaCCTGGT‑CGAGCTCGGGGGCTAACAGCGTCGCGAAAAACAGGGCATTTCCAGCTCCAGGCTTGTCCCGTGCCC‑GGGTCCTGAATTATCTCCATTCTTGTGCTGCCCACGCTACGGACTGCGCAAGATGTTCAGTCAGTCTCGCCTTTCTTTCGGGGCTGTGTTAGCATTTGCCATTCGTGTTTCCTCCTTCCAGCGTTTCATAGCCTTCCAATGATTTATCAGGTCTTAGCCCGAAAATGGCGCCCACaa                                                 <  1:626030/251‑1 (MQ=255)                                                                                                                                                                                                cTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAAcc                                              >  1:246879/1‑251 (MQ=255)                                                                                                                                                                                                 tGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGACGCCCACAAACCt                                             >  1:585591/1‑251 (MQ=255)                                                                                                                                                                                                         actcGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGTGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAGCCTTTGCTGa                                      <  1:148784/250‑1 (MQ=255)                                                                                                                                                                                                           tcGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGAcg                                    >  2:273543/1‑251 (MQ=255)                                                                                                                                                                                                               cggcgAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGAcgtcgt                                <  1:400280/251‑1 (MQ=255)                                                                                                                                                                                                                   gAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGc                                                                    >  2:627616/1‑211 (MQ=255)                                                                                                                                                                                                                   gAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCGCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGc                                                                    <  1:627616/211‑1 (MQ=255)                                                                                                                                                                                                                       aGCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAgg                        >  2:129988/1‑251 (MQ=255)                                                                                                                                                                                                                        gCGTCTCGAAAAACAGGGCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAgg                        >  2:196156/1‑250 (MQ=255)                                                                                                                                                                                                                                  aaaCAGGGCATTAACAGCTACAGTCTTGTCCCGTTCAC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGCAGTCGGCCAGGAACATGTGCTg             >  2:583209/1‑251 (MQ=255)                                                                                                                                                                                                                                  aaaCAGGACATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGATATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTg             >  1:487410/1‑251 (MQ=255)                                                                                                                                                                                                                                     cAGGGCATTACCAGCTACAGTCTTGTCCCGCTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCACTGAGTTAACAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGCCGTCGGCCAGGACCATGTGCTGAcc          >  1:573012/1‑251 (MQ=255)                                                                                                                                                                                                                                       gggCATTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAAACTTTGCTGACGTCGACGGCCAGGAACATGAGCTGACCGc        >  1:343589/1‑251 (MQ=255)                                                                                                                                                                                                                                           aTTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTg    <  1:28200/251‑1 (MQ=255)                                                                                                                                                                                                                                           aTTACCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTg    >  2:193710/1‑251 (MQ=255)                                                                                                                                                                                                                                              aCCAGCTACAGTCTTGTCCCGTTCCC‑GGGCCATTAATTAGCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTAGCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGc  <  2:281970/250‑1 (MQ=255)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                AAACCATCAGTGAAACTTACGACCTGGAATACGGCACCGATCAGCTGGAGATCCACGTTGATGCCATCAAACCGGGCGACAAAGTTCTGGTGGTGGACGACCTGCTGGCAACCGGCGGCACTATCGAAGCGACCGTTAAACTGATCCGTCGTCTGGGTGGTGAAGTGGCTGACGCTGCGTTCATTATCAACCTGTT‑CGATCTCGGCGGCGAACAGCGTCTCGAAAAACAGGGCATTACCAGCTACAGCCTTGTCCCGTTCCC‑GGGCCATTAATTATCGCCAGTCTTGTGCTGCCCACGCTACGGACAGCACAAGATGTGCATTCAGCCTCGCCGTTCTGACGGGGCTGTGTTAGCATTACCCCTTCGTGAATCCACCTTCCAGCGTTTCAGAGCCTGCCAATGAGTTATCAGGTCTTAGCCCGAAAATGGCGCCCACAAACCTTTGCTGACGTCGTCGGCCAGGAACATGTGCTGACCGCACTGGC  >  NC_000913/491692‑492177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑