Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 455,915 T→C G261G (GGT→GGC)  tig → trigger factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913455,9150TC95.2% 302.3 / 0.3 104G261G (GGT→GGC) tigtrigger factor
Reads supporting (aligned to +/- strand):  ref base T (1/4);  new base C (49/49);  total (50/54)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

Only 100 of 104 total aligned reads displayed.

GGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGCTAAAC  >  NC_000913/455670‑456159
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
ggTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGTGCTTGGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATTCCACGCAGAAAACCGGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTTAAGAGCGTGAACTGCCGGAACTGACTGCATAATTCATCAAACGCTGCTGCGTTGAAGATGGCTCCGt                                                                                                                                                                                                                                                 <  2:566588/251‑1 (MQ=255)
    aaGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGa                                                                                                                                                                                                                                              >  1:167009/1‑250 (MQ=255)
     aGCGTCTGATTTCGTACTGGCGATGGGACATGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCAACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTTAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCGCCGTAGAAg                                                                                                                                                                                                                                            <  1:56923/251‑1 (MQ=255)
       cGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGt                                                                                                                                                                                                                                          >  1:200070/1‑251 (MQ=255)
            gATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTgcg                                                                                                                                                                                                                                     >  1:129077/1‑251 (MQ=255)
            gATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGAAGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTggg                                                                                                                                                                                                                                     >  1:363822/1‑249 (MQ=255)
             aTTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGATTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTgcgc                                                                                                                                                                                                                                    >  2:123426/1‑251 (MQ=255)
              tttCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGCCGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAACGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTgcgcg                                                                                                                                                                                                                                   >  2:615561/1‑251 (MQ=255)
                  gTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGa                                                                                                                                                                                                                               <  2:285261/251‑1 (MQ=255)
                    aCTGGCGATGGGCCAGGGTCGAATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGTCGGAACTGACTGCAGAATTCATCAATCGTTTCGGCGTTGAAGATTGCTCCGGAGAAGGTCTGCGCGCTGaa                                                                                                                                                                                                                              <  2:572917/250‑1 (MQ=255)
                           aTGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTa                                                                                                                                                                                                                       >  1:121556/1‑250 (MQ=255)
                            ggggCCAGGGTCGTATGAGCCCGGGCTTTGAAGACGGTATCAAATGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCGGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTaaa                                                                                                                                                                                                                     <  2:554892/250‑1 (MQ=255)
                                ccAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAAc                                                                                                                                                                                                                  >  1:130671/1‑250 (MQ=255)
                                ccAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAAc                                                                                                                                                                                                                  >  1:527456/1‑250 (MQ=255)
                                 cAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTTGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAACAACAt                                                                                                                                                                                                                >  2:597788/1‑251 (MQ=255)
                                 cAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTGCGGCGTTGAAGATGGCTCCGGAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACAt                                                                                                                                                                                                                <  2:119493/251‑1 (MQ=255)
                                    ggTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATgg                                                                                                                                                                                                              >  1:518716/1‑250 (MQ=255)
                                       cGTATGAGCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAgcg                                                                                                                                                                                                          <  1:538711/251‑1 (MQ=255)
                                                ccGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGa                                                                                                                                                                                                  >  1:322125/1‑250 (MQ=255)
                                                 cGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAag                                                                                                                                                                                                <  2:236758/251‑1 (MQ=255)
                                                             aCGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATTCCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGGCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGt                                                                                                                                                                                    <  2:572063/251‑1 (MQ=255)
                                                                gTATCAAAGGCCACAAATCTGGCGAAGAGTTCACCATCGCCGTGACCTTCCCGGCAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAAc                                                                                                                                                                                 >  2:356839/1‑251 (MQ=255)
                                                                  aTCAAAGGCCACAAAGCGGGCGAAGAGTTCACAATCGACGGTACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGATCCGTAGAAGGGCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGTTGAAGAGCGCCATCCGTAACcg                                                                                                                                                                               <  2:517148/251‑1 (MQ=255)
                                                                    cAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACcgcg                                                                                                                                                                             <  1:107992/251‑1 (MQ=255)
                                                                         gCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAg                                                                                                                                                                        <  2:17272/251‑1 (MQ=255)
                                                                           cacaAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGtc                                                                                                                                                                      >  1:489184/1‑251 (MQ=255)
                                                                             caAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAATCCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGtctc                                                                                                                                                                    <  1:251573/251‑1 (MQ=255)
                                                                             aaaaGCGGGCGAAGAGTTCAGCAACGACGGGACATTATCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGAAGCGAAACGCGATATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCTGGAACTGACTGAAGAAGTCATCAACCGTTACGGCGTTGAAGAGGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCTAGCCGTAACCGCGTTAAGtctc                                                                                                                                                                    <  2:146445/250‑1 (MQ=255)
                                                                              acaGCTGGCGAAGAGTTCACCATCGACATGTCCTTCCCGGAAGAATACCACGCAGAAAACCTGAACGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGACAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGCAGTGCGGACAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTCAGtctcc                                                                                                                                                                   >  2:560406/3‑250 (MQ=255)
                                                                              aaaGCTGGCGAAGAGTTCACCATCGACGTGCCCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTCTCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAAAGCGCCATACGTAACCGCGTTAAGtctc                                                                                                                                                                    >  2:425401/1‑250 (MQ=255)
                                                                               aaGCTGGCGCAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGtctc                                                                                                                                                                    >  2:568805/1‑249 (MQ=255)
                                                                                aGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAgg                                                                                                                                                                 >  1:138680/1‑251 (MQ=255)
                                                                                  cTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGATCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCg                                                                                                                                                               >  2:608845/1‑251 (MQ=255)
                                                                                  cTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCg                                                                                                                                                               >  1:65518/1‑251 (MQ=255)
                                                                                   tGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGCAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTACGTCTCAGGCg                                                                                                                                                               >  2:476644/1‑250 (MQ=255)
                                                                                    ggCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGa                                                                                                                                                              <  1:493946/250‑1 (MQ=255)
                                                                                       gAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCAACAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCg                                                                                                                                                           >  1:252755/1‑250 (MQ=255)
                                                                                         agaattCACCATCCAAGTGACCTTCCCGGAAGAATACCACACAGAAAACCTGAAAGGAAAAGCAGAGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGCGAACTGCCGGAACTGACTGCAGAATTCATAAAACGCTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGAGCGCTGAATCGCGTAAAAAAATGGAGCGCGAGCAGAGGAGCGCCATCAGTAACCGCGTTAAGCCTCAGGCgctaga                                                                                                                                                          >  2:369589/5‑244 (MQ=255)
                                                                                               cACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGCAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTgg                                                                                                                                                  >  2:434840/1‑251 (MQ=255)
                                                                                               cACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTgg                                                                                                                                                  <  2:550683/251‑1 (MQ=255)
                                                                                                 ccATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGt                                                                                                                                                 >  2:119413/1‑250 (MQ=255)
                                                                                                      gACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGc                                                                                                                                           >  1:429420/1‑251 (MQ=255)
                                                                                                        cGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTa                                                                                                                                         <  1:558563/251‑1 (MQ=255)
                                                                                                              cTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAGATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTATGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCCAACGAcc                                                                                                                                   >  1:535662/1‑250 (MQ=255)
                                                                                                                  ccGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCg                                                                                                                                >  2:158950/1‑250 (MQ=255)
                                                                                                                       aaaaTACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAAATCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAAAATTCATCAAACGTTACGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTAc                                                                                                                          <  2:122252/249‑1 (MQ=255)
                                                                                                                        gaaTACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTAcc                                                                                                                         >  2:112723/1‑251 (MQ=255)
                                                                                                                           tACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGGCTGGTAAAAGCTAACGACATCGACGTACCGGc                                                                                                                      >  2:455643/1‑251 (MQ=255)
                                                                                                                                   aGAAACCCTGAAAGGTAAAGCAGCGAAAGTCGCTAGCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACAGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCTATCCGTAACCGCGTTAAGTCTCAGGCGATGGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGa                                                                                                              <  1:541937/251‑1 (MQ=255)
                                                                                                                                    gAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGAt                                                                                                             <  1:92944/251‑1 (MQ=255)
                                                                                                                                     aaaaCCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACAGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATc                                                                                                            >  1:180583/1‑251 (MQ=255)
                                                                                                                                      aaaCCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCg                                                                                                           >  2:351230/1‑251 (MQ=255)
                                                                                                                                        aCCTGAAAGGAAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGCCATCGACGTACCGGCTGCGCTGATCGAc                                                                                                         >  1:426379/1‑251 (MQ=255)
                                                                                                                                          ccgAAAGGTAAAGCAGCGAAAATCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACTTTTCGGCGTTGAAGATGGCTCCGTAGAAGGGCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGCGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACat                                                                                                       <  1:478452/249‑2 (MQ=255)
                                                                                                                                            taaaGGTAAAGCAGCGAAATTCGCTAGAAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGc                                                                                                      <  2:473157/249‑1 (MQ=255)
                                                                                                                                              aaGGTAAAGCAGCGAAATTCGCTATCAACCGGAAGAAAGTTGAAGAGCGTGAACCGCCGGAACTGACGGTAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCGCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGAGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCGGCGCTGATCGACAGCGaa                                                                                                   <  2:330988/251‑1 (MQ=255)
                                                                                                                                              aaGGTAAAGAAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGa                                                                                                    >  1:452827/1‑250 (MQ=255)
                                                                                                                                                 gTAAAGCAGCGACATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCTTCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGCCATCGACGTACCGGCTGCGCGGATCGACAGCGACATc                                                                                                >  2:335248/1‑251 (MQ=255)
                                                                                                                                                   aaaGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGa                                                                                              <  2:151231/251‑1 (MQ=255)
                                                                                                                                                      gccgcGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGt                                                                                           <  2:167009/248‑1 (MQ=255)
                                                                                                                                                        agcGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACGGCCGGAACTGACTGCAGAATTCATCAAACGTTGCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAAGCGACGTTc                                                                                         <  2:56987/251‑1 (MQ=255)
                                                                                                                                                          cGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTg                                                                                       <  1:455643/251‑1 (MQ=255)
                                                                                                                                                            aaaTTCGCTAGCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAAGTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCg                                                                                     <  1:425401/251‑1 (MQ=255)
                                                                                                                                                                acgcgaTCAACCTGAAGAAAGTTGAAGAGCGTGACCTGCCGGAACTGCCTGCAGAATTCATCACACGTTTCGGCGTTGCAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGCAGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGc                                                                                 >  2:309531/6‑251 (MQ=255)
                                                                                                                                                                       cAACCTGAAGAAAGTTGAAGCGCGTGAACTGCCGGAACCGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGACGGTCTGGTAAAAGCTAACGACATCGCCGTACAGGCTGCGCTGATCGACAGCGACATCGACGTTCTGCGTCGCCAGGCTg                                                                          >  2:359781/1‑251 (MQ=255)
                                                                                                                                                                       cAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCt                                                                           <  2:121556/250‑1 (MQ=255)
                                                                                                                                                                       cAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTg                                                                          <  1:615561/251‑1 (MQ=255)
                                                                                                                                                                          ccTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTTGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGcac                                                                       <  2:330920/251‑1 (MQ=255)
                                                                                                                                                                          ccTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGcac                                                                       >  1:58414/1‑251 (MQ=255)
                                                                                                                                                                           cTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAgg                                                                                                                                                                 <  2:154396/160‑1 (MQ=255)
                                                                                                                                                                           cTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAgg                                                                                                                                                                 >  1:154396/1‑160 (MQ=255)
                                                                                                                                                                                gaaAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGtt                                                                 >  2:459293/1‑251 (MQ=255)
                                                                                                                                                                                  aaGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGAACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTc                                                               >  2:180382/1‑251 (MQ=255)
                                                                                                                                                                                     ttGAAGAGCCTGAACTGCCGGAACTGACTGCATCATTCCTCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAATGTCTGCGCGCTTAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCTTCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGTCGTTCTGCGTCTTCAGGCTGCACAGCGGTTCgt                                                             <  2:52772/250‑2 (MQ=255)
                                                                                                                                                                                       gAAGAGCGTGATCTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGGACCGGCTGCGCTGATCGACAGCGAAATCGACGGTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTgg                                                          <  1:275264/251‑1 (MQ=255)
                                                                                                                                                                                          gagCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCaa                                                       <  1:67913/251‑1 (MQ=255)
                                                                                                                                                                                           agCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCTCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCGAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAAc                                                      <  1:370461/251‑1 (MQ=255)
                                                                                                                                                                                            gCGTGCACTGCCGGCACTGACTGCAGAATTCATCAACCGTTTCGGCGTTGCAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTACAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGCTCGAAGGTCTGGTAAAAGCTCACGACATCGACGTACAGGCTGCGCTGATCGACAGCGAACTCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCgggggaaa                                                       >  2:297862/1‑243 (MQ=255)
                                                                                                                                                                                                gAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAg                                                                     <  2:42981/231‑1 (MQ=255)
                                                                                                                                                                                                gAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAg                                                                     >  1:42981/1‑231 (MQ=255)
                                                                                                                                                                                                        ggAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGGCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGctc                                         <  2:426379/251‑1 (MQ=255)
                                                                                                                                                                                                          aaCTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTg                                       >  1:168078/1‑251 (MQ=255)
                                                                                                                                                                                                            ctgactgCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTgg                                      >  1:430860/1‑250 (MQ=255)
                                                                                                                                                                                                              gactgCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGaa                                    <  2:208091/250‑1 (MQ=255)
                                                                                                                                                                                                              gactgCAGAATGCATCAAACGTTTCGGCGTTGAAGATGGCTCCGGAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGa                                     <  1:560406/249‑1 (MQ=255)
                                                                                                                                                                                                               actgCAGAATTCATCAAACGTTTCGGCGTGGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACt                                  <  2:200070/251‑1 (MQ=255)
                                                                                                                                                                                                                  gCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTg                                 <  1:596463/249‑1 (MQ=255)
                                                                                                                                                                                                                  gCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGCCCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGcc                               >  2:447899/1‑251 (MQ=255)
                                                                                                                                                                                                                       aTTCATCAAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCcgcgcg                          <  1:568805/251‑1 (MQ=255)
                                                                                                                                                                                                                          catcaAACGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAAc                       <  2:138680/251‑1 (MQ=255)
                                                                                                                                                                                                                               aaCGTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTc                  <  2:430860/251‑1 (MQ=255)
                                                                                                                                                                                                                                 cGTTTCGGTGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCga                <  2:274493/251‑1 (MQ=255)
                                                                                                                                                                                                                                  gTTTCGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCGCTGGAACTGCCGCGCGAACTGTTCgaa               <  2:208857/251‑1 (MQ=255)
                                                                                                                                                                                                                                      cGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAACCATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAACGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCTACGAAAAAAAAGCTCTGGAACTGCCGCGCGAACTGTTCgaagca            >  2:299289/1‑248 (MQ=255)
                                                                                                                                                                                                                                      cGGCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACAGAGTTAAGTATCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCAAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGCACTGCCGCGCGACCTGTTCgaagaa            >  2:208995/1‑250 (MQ=255)
                                                                                                                                                                                                                                        gCGTTGAAGATGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGATCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAg         <  1:608845/251‑1 (MQ=255)
                                                                                                                                                                                                                                          gTTGAAGCTGGCTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACCGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGc       >  2:457350/1‑251 (MQ=255)
                                                                                                                                                                                                                                          gTTGAAGATGGCGCCGTAGAAGGTGTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAATAGCGCCATCCGTAACCGCGGTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCGCAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGGACTGTTCGAAGACCAGGc       <  2:506419/251‑1 (MQ=255)
                                                                                                                                                                                                                                            tGAAGATGGCCCCGTAGAAGGTCTGCGCGCTGAAGTGCGGAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGCt      <  1:131925/250‑1 (MQ=255)
                                                                                                                                                                                                                                               aGATGGCTCCGTAGAAGGTCTGAGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGCTAAAc  <  1:147093/251‑1 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
GGTAAAGCGTCTGATTTCGTACTGGCGATGGGCCAGGGTCGTATGATCCCGGGCTTTGAAGACGGTATCAAAGGCCACAAAGCTGGCGAAGAGTTCACCATCGACGTGACCTTCCCGGAAGAATACCACGCAGAAAACCTGAAAGGTAAAGCAGCGAAATTCGCTATCAACCTGAAGAAAGTTGAAGAGCGTGAACTGCCGGAACTGACTGCAGAATTCATCAAACGTTTCGGCGTTGAAGATGGTTCCGTAGAAGGTCTGCGCGCTGAAGTGCGTAAAAACATGGAGCGCGAGCTGAAGAGCGCCATCCGTAACCGCGTTAAGTCTCAGGCGATCGAAGGTCTGGTAAAAGCTAACGACATCGACGTACCGGCTGCGCTGATCGACAGCGAAATCGACGTTCTGCGTCGCCAGGCTGCACAGCGTTTCGGTGGCAACGAAAAACAAGCTCTGGAACTGCCGCGCGAACTGTTCGAAGAACAGGCTAAAC  >  NC_000913/455670‑456159

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑