Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	271,964	C→T	pseudogene (201/427 nt)	insN →	IS911A regulator fragment

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	271,964	0	C	T	100.0%	62.5 / NA	27	pseudogene (201/427 nt)	insN	IS911A regulator fragment
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (17/10);  total (17/10)

TGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑ACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGC  >  NC_000913/271727‑272073                                                                                                                                                                                                                                               |                                                                                                              cgcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCa                                                                                                   <  1:458651‑M1/215‑1 (MQ=255)  gcattgaaatggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCa                                                                                                   <  1:231200‑M1/215‑1 (MQ=255)          atggagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCg                                                                                         >  2:223867‑M1/27‑251 (MQ=255)             gagaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGcc                                                                                       <  1:373173‑M1/227‑1 (MQ=255)               gaatgaaatattaaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCcaca                                                                                    >  1:255084‑M1/22‑251 (MQ=255)                 atgaaatattaaaaaggctACCGCGCGCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGTCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAAt                                                                                  >  2:588519‑M1/20‑251 (MQ=255)                   gaaatattaaaaaggctACCGCGCTCTTGATGTCAGCCTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGGGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCGGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAAt                                                                                  <  1:455198‑M1/232‑1 (MQ=255)                          taaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAacccg                                                                         <  1:575213‑M1/241‑2 (MQ=255)                          taaaaaggctACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCACGATAATCGGGAAACTCAGAGCGCATTATCCGGTGGTCACACTCTGCCATGTGTTCGGGGTTCAGCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTGGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAAccc                                                                          >  2:422112‑M1/11‑250 (MQ=255)                                   tACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCAcaatggcaacccggcgaggct                                                                 <  1:334191‑M1/249‑10 (MQ=255)                                     ccGCGATCTTGATGTCAGACTCCATGCACAGTTCTCGGTAATAGGAAAACTCAGGGCGCATTATCCTGTGCTCACACTCTGCATTGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAACAACAGTCCTGAAAAACCAGCCGGCAGACGGGCTGTATTACGCAGTAAGGTACTTGAGAC‑ACATGGCATCAGCCACGGTTCGGCCGGAGCCAGACGCATCGCCACAATGGCAACCCGGAGAggcaac                                                               >  2:561369/1‑246 (MQ=12)                                       gcgcTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCGACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCAGCGCCACAATGGCAACCCGGAGAGGCTACCAg                                                            <  2:90080/251‑1 (MQ=18)                                              tGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGTCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATgggac                                                     >  2:468080/1‑249 (MQ=18)                                                aTGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GATACATGGAATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGATACCAGATGGGacgc                                                   >  1:560112/1‑251 (MQ=18)                                                     aGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCGTGTGTTCGGGGTTCATCGCAGCAGCTCCAGATACTGGAAAAACCGTCCTGAAAAACAAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCAGGGTTCGGCCGGAGCAAGAAGCATAGCCACAATGGCAAACCGGAGAGGCTACCAGATGGGACGCTGGCt                                              >  1:595105/1‑251 (MQ=17)                                                      gACTCCATGAACAGTTCTCACTAATCGGGAAACTCAGAGCGCATTATCCTGTGGACACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACACTGGCAACCCGGAGAGGCTCCCCGATGGGACGCTGGCtt                                             >  2:78851/1‑251 (MQ=14)                                                          cccTGAACCGTTCTCGATAATCGGGAGACTCAGAGCGCATTTTCCTGTGGTCACACTCTGCCAGGTGTTCGGGGTTCATCGCAGCAGCTACAGAGACTGGAAAACCCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGTCAACCCGGAGAGGCAACCAGATGGGACGCTGGCTTGCTg                                         <  2:455198/251‑1 (MQ=14)                                                          cccTGAACAGTTCTCGATAAACGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCAGCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTtgcttgct                                          <  2:414735/250‑1 (MQ=17)                                                              gAACAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGc                                       >  2:25465/1‑249 (MQ=17)                                                                 cAGTTCTCGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGACAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAggcc                                  >  2:254822/1‑248 (MQ=14)                                                                     tctcGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCAt                               <  2:161756/250‑1 (MQ=14)                                                                      atcGATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATg                              >  2:350575/2‑250 (MQ=14)                                                                         gATAATCGGGAAACTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGaa                            >  1:470428/1‑249 (MQ=14)                                                                                      cTCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGt             >  1:467952/1‑251 (MQ=11)                                                                                       tCAGAGCGCATTATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTc            >  2:193104/1‑251 (MQ=11)                                                                                                 ttATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTcagc  >  2:72973/1‑251 (MQ=11)                                                                                                  tATCCTGTGGTCACACTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAGATACTGGAAAAACCGTCCTGAAAAACCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑GCTACATGGCATCAGCCACGGTTCGGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCGGAGAGGCTACCAGATGGGACGCTGGCTTGCTGGCAGGCTCATGAAAGAGCTGGGGCTGGTCAGCTGTcagc  >  1:573826/1‑250 (MQ=11)                                                                                                                                                                                                                                               |                                                                                                              TGAAAGGGGTGTTGCATTGACAGATTGAATCTACAACCGCGCTCTTGATGTCAGACTCCCTGAACAGTTCTCGATAATCGGGAAACTCAGGGCGCGTTATCCTGTGGCCACTCTCTGCCATGTGTTCGGGGTTCATCGCAGCAGCTACAAATACTGGAAAAACCGTCCTGAAAAGCCAGACGGCAGACGGGCTGTATTACGCAGTCAGGTACTTGA‑ACTGCATGGCATCAGCCACGGCTCTGCCGGAGCAAGAAGCATCGCCACAATGGCAACCCAGAGAGGATACCAGATGGGGCGCTGGCTTGCTGGCAGACTCATGAAAGAGCTGGGGCTGGTCAGTTGCCAGC  >  NC_000913/271727‑272073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑