Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	193,327	C→T	D152D (GAC→GAT)	frr →	ribosome‑recycling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	193,327	0	C	T	100.0%	232.3 / NA	70	D152D (GAC→GAT)	frr	ribosome‑recycling factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (32/38);  total (32/38)

TCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTCGACCGGCTCGATTGGTTGCAGCA  >  NC_000913/193084‑193569                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   tCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTACCGAGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGcc                                                                                                                                                                                                                                             >  1:104467/1‑251 (MQ=255)  cGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGcc                                                                                                                                                                                                                                             <  1:94512/250‑1 (MQ=255)     tCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGGGAAGCAGAACAAGCGCGTGTGGCAGTACGTAACGTGCGTCGGGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGAACGCCGtt                                                                                                                                                                                                                                          <  2:625883/250‑1 (MQ=255)               cGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCTCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGAGAAAGAGATCAGCGAAGACGATGAGCGCCGTTCTCAGGACGAt                                                                                                                                                                                                                               <  2:404211/251‑1 (MQ=255)                 gctgatGAAAAAGCGATTCTGGGTTCCGATCTTGGCCTGAACCCGAATTCTGCGGGTAGCTACCGTCGTTTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCTAAATCGGTCG‑TGGTGAAGCAGAACAAGCTCGTGTTGCAGTACGTAACGTGGTTCGTGTCGCGAACGACAAAGTGTAAGCAATGGTGAAAGATAAATAGATCAGCGAAGACGATGATCGCCGTTCTCAGGTCGATGt                                                                                                                                                                                                                             <  2:629289/246‑1 (MQ=255)                       gAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGATGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGa                                                                                                                                                                                                                        <  2:86544/250‑1 (MQ=255)                              ggATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAactgact                                                                                                                                                                                                                <  2:16433/250‑1 (MQ=255)                                 ttATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCCAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGCACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAACGATAAAGAGATCAGCGAAGCCGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGCCTGAt                                                                                                                                                                                                             >  2:78830/1‑251 (MQ=255)                                 ttATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAAACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGAt                                                                                                                                                                                                             >  2:178622/1‑251 (MQ=255)                                  tATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGAt                                                                                                                                                                                                             <  2:506661/250‑1 (MQ=255)                                   aTGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACATAAACTGACTGAtgc                                                                                                                                                                                                           >  2:154481/1‑251 (MQ=255)                                          ccGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATc                                                                                                                                                                                                    >  1:504262/1‑251 (MQ=255)                                                 tggccggacccctacctcgtcGGGTAGCCACATCCGTGTTCTGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TTGTGAAGCAGAACAAGCGTGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGTCGATGATCGCCGTTCGCAGGACGATGTACAGAAACTGACTGATGCTGCAATc                                                                                                                                                                                                    <  2:363780/224‑1 (MQ=255)                                                 tGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCaa                                                                                                                                                                                                  <  2:246066/246‑1 (MQ=255)                                                    ccTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTg                                                                                                                                                                                          <  2:321196/251‑1 (MQ=255)                                                    ccTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTg                                                                                                                                                                                          <  1:25964/251‑1 (MQ=255)                                                       gAACCCGAACTCTGCGGGTAGCGACAGCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGGTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAg                                                                                                                                                                                       <  2:292050/251‑1 (MQ=255)                                                             gAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGgcgc                                                                                                                                                                                 >  1:448104/1‑251 (MQ=255)                                                                        gTAGCGACATCCGTGTTCCGCTGCCGCCGCGGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑GGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTTGCAGACaat                                                                                                                                                                      <  2:3771/251‑2 (MQ=255)                                                                               cATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGc                                                                                                                                                                 >  1:447839/1‑249 (MQ=255)                                                                                  ccGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAAc                                                                                                                                                            <  1:541732/251‑1 (MQ=255)                                                                                  ccGTGTGCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGaa                                                                                                                                                             <  1:553571/250‑1 (MQ=255)                                                                                    gtgtTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATTAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAATCAGAACTg                                                                                                                                                          <  2:443855/251‑1 (MQ=255)                                                                                    gtgtTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTg                                                                                                                                                          <  2:179497/251‑1 (MQ=255)                                                                                    gtgtTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTg                                                                                                                                                          >  1:267282/1‑251 (MQ=255)                                                                                        tCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATTTGACCAAAATCTTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTTAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATg                                                                                                                                                       <  1:56931/250‑1 (MQ=255)                                                                                               ccgccgCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTg                                                                                                                                               <  1:86861/251‑1 (MQ=255)                                                                                                     gtGACGGAAGAACGTCGTAAAGATCTGAACAAAATCGTTCGGTGG‑GAAGCAGAACAAGCGCGTGGTGCAGGACGTAACGTTCGGCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCt                                                                                                                                         <  1:262376/250‑1 (MQ=255)                                                                                                                aCGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACaaa                                                                                                                              <  1:172860/251‑1 (MQ=255)                                                                                                                aCGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACAGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACaaa                                                                                                                              <  2:142679/251‑1 (MQ=255)                                                                                                                    cgtAAAGATCTGACCAAAATCGTTCG‑TGGTGAATCAGAACAAGCGCGTTTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATTATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAc                                                                                                                           <  1:313299/250‑1 (MQ=255)                                                                                                                     gtAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgc                                                                                                                         >  1:539707/1‑251 (MQ=255)                                                                                                                       aaaGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAAcgccg                                                                                                                       <  1:36077/251‑1 (MQ=255)                                                                                                                           aTCTGGCCAAAATCGTTCG‑TGGTGAAGCCGAACAAGCGCGTGATGCAGTACGTACCGTGCGTCGTGACGCGAACGACCAAGTGAAAGCACTGTTGCAAGATAAAGAGATCAGCGAAGACGATGCTCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGTCGCTGGCAGACAAAGCAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTc                                                                                                                    >  2:579839/1‑250 (MQ=255)                                                                                                                               gACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGt                                                                                                                 >  1:8685/1‑249 (MQ=255)                                                                                                                                 ccAAAATCGTTCG‑TGGTGAAGCAGAACACGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGCAAGCACTGTTGAAAGATAAAGAGATCAGCGACGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGt                                                                                                                 >  2:32470/1‑247 (MQ=255)                                                                                                                                 ccAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGt                                                                                                                 >  1:376872/1‑247 (MQ=255)                                                                                                                                 ccAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGt                                                                                                                 >  1:269651/1‑247 (MQ=255)                                                                                                                                      aTCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAAGCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTg                                                                                                        <  2:51452/251‑1 (MQ=255)                                                                                                                                      aTCGTTCG‑GGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTg                                                                                                        <  2:511426/251‑1 (MQ=255)                                                                                                                                         gttcg‑tGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCgg                                                                                                     >  1:193574/1‑251 (MQ=255)                                                                                                                                             g‑tggtgAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGCCGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCg                                                                                                 >  2:165858/1‑251 (MQ=255)                                                                                                                                                ggtgAAGCAGAACAAGAGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGCTGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGc                                                                                               >  2:218059/1‑251 (MQ=255)                                                                                                                                                 gtgAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCt                                                                                              <  2:8685/251‑1 (MQ=255)                                                                                                                                                      gCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTggcgg                                                                                         <  1:210633/251‑1 (MQ=255)                                                                                                                                                           aCAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGttt                                                                                    <  2:359573/251‑1 (MQ=255)                                                                                                                                                            cAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGtttt                                                                                   <  1:582770/251‑1 (MQ=255)                                                                                                                                                              aGCGCGTGTTGCAGTACGTAACGTGCGTAGGGACGCGAACGACAAAGTGAAAGCACTGTGTAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGc                                                                                 <  2:25127/251‑1 (MQ=255)                                                                                                                                                                          aGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTTTTCttt                                                                     >  2:260702/1‑249 (MQ=255)                                                                                                                                                                             acggaaCGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTGCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtct                                                                   <  2:394468/246‑1 (MQ=255)                                                                                                                                                                               gTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTg                                                                                                                                                                               >  1:432608/1‑140 (MQ=255)                                                                                                                                                                               gTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTg                                                                                                                                                                               <  2:432608/140‑1 (MQ=255)                                                                                                                                                                                 aaCGTGCGTCGTGACGCGAACGACAACGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAAct                                                              >  2:96048/1‑251 (MQ=255)                                                                                                                                                                                 aaCGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAAct                                                              >  1:615157/1‑251 (MQ=255)                                                                                                                                                                                 aaCGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAAc                                                               <  1:441994/250‑1 (MQ=255)                                                                                                                                                                                 aaCGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAAc                                                               <  1:446906/250‑1 (MQ=255)                                                                                                                                                                                                        cAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGCTGCGGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTGTTGCTTTTTATTCTATCTCAACTCTGGATGTTTCCTGAAGCAACTc                                       >  1:347920/1‑251 (MQ=255)                                                                                                                                                                                                        cAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGAGAAGACGATGATCGCCGTTCTCAGGACGATGAACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATGTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGAAGTTTCATGAAGCAACTc                                       >  2:500028/1‑251 (MQ=255)                                                                                                                                                                                                         aaaGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTc                                       >  1:364933/1‑250 (MQ=255)                                                                                                                                                                                                              gAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCAGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCAtt                                 >  2:41957/1‑251 (MQ=255)                                                                                                                                                                                                                  gCACTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTgg                             <  1:35342/251‑1 (MQ=255)                                                                                                                                                                                                                    aCTGTTCACAGATAAAGAGATCAAAGAAGACGATGATCGCCGTTCTCAGAACGCTGAACAGAAACTGACTGCTGCTGCAATCAAGAACATTTAAGCGGCGCTGGCAGACACTGACGCCGAACTGATGCAGTACTGATTTCTTGAACGACAAAAACGCCGCTAAGTAGATCCTTGCGACTCGGCTGGCAGCGTTTTGCTTTTTATTCTGTCTCCACTCTGGATGTTTCATGTAGCAACTCACCATTcagtgc                           >  2:579076/1‑246 (MQ=255)                                                                                                                                                                                                                     cTGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCt                          >  2:433029/1‑251 (MQ=255)                                                                                                                                                                                                                      tGTTGAAAGATAAAGAGATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTc                         >  1:488943/1‑251 (MQ=255)                                                                                                                                                                                                                                   agagATCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTCGCCCGGCTCGAtt            >  2:164005/1‑251 (MQ=255)                                                                                                                                                                                                                                       aTCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTCGACCGGCTCGATTgggt        >  1:187721/1‑249 (MQ=255)                                                                                                                                                                                                                                       aTCAGCGAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACGGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGtctc                                                                  <  1:628367/193‑1 (MQ=255)                                                                                                                                                                                                                                       aTCAGCGAAGACGATGATCGACGTTCTCAGGAAGATGTACCGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGCTGCAGTTCTGATTTCTTGACCGACAAAAACGCCGCTCAGTAGATCATTGCGGATCGGCTGGCGGCGTTTTGCTTTTTCTTCTGtctc                                                                  >  2:628367/1‑193 (MQ=255)                                                                                                                                                                                                                                             gAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTCGACCGGCTCGATTGGTTgcagca  <  1:413189/251‑1 (MQ=255)                                                                                                                                                                                                                                             gAAGACGATGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTCGACCGGCTCGATTGGTTgcagc   >  1:614890/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   TCGTTCAATGTCTCCGGCCGTTGAAAAAGCGATTATGGCGTCCGATCTTGGCCTGAACCCGAACTCTGCGGGTAGCGACATCCGTGTTCCGCTGCCGCCGCTGACGGAAGAACGTCGTAAAGATCTGACCAAAATCGTTCG‑TGGTGAAGCAGAACAAGCGCGTGTTGCAGTACGTAACGTGCGTCGTGACGCGAACGACAAAGTGAAAGCACTGTTGAAAGATAAAGAGATCAGCGAAGACGACGATCGCCGTTCTCAGGACGATGTACAGAAACTGACTGATGCTGCAATCAAGAAAATTGAAGCGGCGCTGGCAGACAAAGAAGCAGAACTGATGCAGTTCTGATTTCTTGAACGACAAAAACGCCGCTCAGTAGATCCTTGCGGATCGGCTGGCGGCGTTTTGCTTTTTATTCTGTCTCAACTCTGGATGTTTCATGAAGCAACTCACCATTCTGGGCTCGACCGGCTCGATTGGTTGCAGCA  >  NC_000913/193084‑193569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑