Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	156,068	A→G	V45A (GTA→GCA)	yadV ←	putative fimbrial chaperone YadV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	156,068	0	A	G	100.0%	81.2 / NA	30	V45A (GTA→GCA)	yadV	putative fimbrial chaperone YadV
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (8/22);  total (8/22)

TTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATAC  >  NC_000913/155823‑156298                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       tttCTGCATCTGGTTGTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATGGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGGAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCGAGTATCTAACCAACTCTGGACAAGCAACGTGTTAGTCCCGTTATTTTCCATACGTGCGtt                                                                                                                                                                                                                                    <  2:590646/250‑1 (MQ=255)      gCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGaca                                                                                                                                                                                                                                <  1:253880/249‑1 (MQ=255)          cTGGTTTTGGTTGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATAGCCAGTATCGAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACttttt                                                                                                                                                                                                                          >  2:134219/1‑251 (MQ=255)                  ggtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTGGCCCACGGTTGGCATCAATACGCGAGACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCt                                                                                                                                                                                                                  <  1:570759/251‑1 (MQ=255)                   gtggAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTGTTTGATCGCtt                                                                                                                                                                                                                 <  2:360990/251‑1 (MQ=255)                           tCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATGTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTtatata                                                                                                                                                                                                          <  2:199385/250‑1 (MQ=255)                            ccAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTGTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTatatat                                                                                                                                                                                                         <  1:301313/250‑1 (MQ=255)                                 aCGTTAAACCAGAACACGCTCTCTCTATCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTAcgcg                                                                                                                                                                                                   <  2:511755/251‑1 (MQ=255)                                        acccgAACACTCTTTCTCTGTCTTGAGGCAGTGAGGTGCGGGCTGGGTACATTAATTTGATTGTTTGCCCACGTTGGGCATCAATACGCGAGACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTAccc                                                                                                                                                                                            <  2:536246/247‑1 (MQ=255)                                           aGAACACGCTCTGCCTGTCTTTAGGCAGTGAGGTGCTGGCTGCGTACATTAATTTGCTTGTTTGCCCACGTTTGGCATCAATACGCGATACTAGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACACGa                                                                                                                                                                                          >  2:428283/1‑250 (MQ=255)                                                                    cAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATg                                                                                                                                                                <  1:282665/251‑1 (MQ=255)                                                                     aGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTCGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGa                                                                                                                                                               <  1:249163/251‑1 (MQ=255)                                                                                                                gTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGt                                                                                                                    <  2:197334/251‑1 (MQ=255)                                                                                                                   tGGCATCAATACGCGAGACTGGCGGCGTAGCAGTAAAAGGGACTGTAATCCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTGTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCACACAGGTTACGAAGCATAAAGCTGTTGTATGttt                                                                                                                  <  2:609602/250‑1 (MQ=255)                                                                                                                               gcgATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTGTTTGATCGCTTTTATATATTACGCGAGTACCCGAAAGGACAATTTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGGTGTATGTTTGGTGTTAAAAAAc                                                                                                     <  1:231245/251‑1 (MQ=255)                                                                                                                                                   gTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATag                                                                                   >  1:222892/1‑249 (MQ=255)                                                                                                                                                               gTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTaaaa                                                                       <  2:187500/249‑1 (MQ=255)                                                                                                                                                                    aCTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGCTCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGa                                                                 >  2:327450/1‑250 (MQ=255)                                                                                                                                                                       gCCAGGCTCAGTGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTGGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGaata                                                              <  2:402028/250‑1 (MQ=255)                                                                                                                                                                       gCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTTGACAAGCAACGGTTTATTCCCTTTATTTTCCAGTCGTGCGTTGACTCTTTTTTGATCGCTTTTATATATTACGCGAGCACCCGAAATGACAATGTCCGCAATATACGATGAACTAAAAGCCATACAGCTTACGAAGCATAAAGCTGTTGTATGTCGGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGaat                                                               <  1:555856/249‑1 (MQ=255)                                                                                                                                                                       gCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACGCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGaat                                                               <  1:333978/249‑1 (MQ=255)                                                                                                                                                                        ccAGGCGCAGCGGTGTCATCGCCATTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCTTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCTAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGTTGTTGTATGTTTGGTGTTAAAAAACATAAACAATCCCTGATAGAGTTAATTAATACAGGGaataat                                                            <  2:389812/251‑1 (MQ=255)                                                                                                                                                                                            gCCAGTATCTAACCAACGCTGGACAAGCAACGGGTTATTCCCTTTATTTGCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATAGAAAATAGAAattattatt                                        <  1:410942/251‑1 (MQ=255)                                                                                                                                                                                              cAGTATCTAACCAACTCTGGACAAGCAACGGGTTCTTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTcc                                      >  2:584068/1‑251 (MQ=255)                                                                                                                                                                                                         cAACTCTGGACAAGCTACGGGTTATTCCCTTTATTTTGCAGACGTGCGTTGACACTATTTTGATCGCTTTTATATATTACGCGAGAGCCCTAAATGACAAGAAGCGCAATAGACTATGAACTAAAAGCCATACAGGTTACGAAGCAGAAAGATGTTGTATGTTTTGTGTTAAAAAACAGAAACCAGCCCTGATAGAGTTAATGAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATattca                           <  2:525846/251‑1 (MQ=255)                                                                                                                                                                                                              ctGGAAAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAAGGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCAGTATattcattc                        <  2:604074/249‑1 (MQ=255)                                                                                                                                                                                                               tgaaccaGCAACGGGTTATTCCCTTTATTTTCCAGACTTGCGTTAACCAATTCTTGATCGCCTTTATCTATTACGCGAGCACCCGAACTGACACTGTCCTCAATACACGTTGTACTAATAGCCATACAGGTTACGAAGCATTAAGTTGTTGTCTGTTTGGTGTTAACAACCATAAATCATCCCTGTTAGAGATAATTAAAACAGGGAATAATATAAACTAGAAATTATTCTTCCCTGCATATTCATttaat                     >  2:42198/7‑251 (MQ=255)                                                                                                                                                                                                                            ggTTATTCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTACCTGGt        >  1:481815/1‑251 (MQ=255)                                                                                                                                                                                                                                  tCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGTGAGTACCCGAAATGACAATGTCCGTAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAAta   <  2:435222/250‑1 (MQ=255)                                                                                                                                                                                                                                  tCCCTTTATTTTCCAGACGTGCGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAAtac  >  2:463896/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                       TTTCTGCATCTGGTTTTGGTGGAACTTCCAGTACGTTAAACCAGAACACGCTCTCTCTGTCTTTAGGCAGTGAGGTGCTGGCTGTGTACATTAATTTGATTGTTTGCCCACGTTTGGCATCAATACGCGATACTGGCGGCGTAGCAGTAAAAGGGACTGTAATACTGCCAGGCTCAGCGTTGTCATCGCCAGTATCTAACCAACTCTGGACAAGCAACGGGTTATTCCCTTTATTTTCCAGACGTACGTTGACACTTTTTTGATCGCTTTTATATATTACGCGAGTACCCGAAATGACAATGTCCGCAATAGACGATGAACTAAAAGCCATACAGGTTACGAAGCATAAAGCTGTTGTATGTTTGGTGTTAAAAAACATAAACCATCCCTGATAGAGTTAATTAAAACAGGGAATAATATAAAATAGAAATTATTATTCCCTGTATATTCATTCAATCAATTTAACTGGTGAATAC  >  NC_000913/155823‑156298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑