Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	154,182	C→T	L415L (CTG→CTA)	htrE ←	putative fimbrial usher protein HtrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	154,182	0	C	T	100.0%	133.6 / NA	45	L415L (CTG→CTA)	htrE	putative fimbrial usher protein HtrE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (23/22);  total (23/22)

ACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAT  >  NC_000913/153936‑154427                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      aCGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGAc                                                                                                                                                                                                                                                   >  1:246527/1‑251 (MQ=255)         aTTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGTCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAGAATGCACCAACTGAAGTATTTAGACCAAGACCt                                                                                                                                                                                                                                           <  2:108585/251‑1 (MQ=255)         aTTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCt                                                                                                                                                                                                                                           <  1:240345/251‑1 (MQ=255)             cGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCATTGAAGGACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACGGAAGTATTTAGACCAATACCTAAc                                                                                                                                                                                                                                        <  2:206513/250‑1 (MQ=255)              ggTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTCATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGACGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACa                                                                                                                                                                                                                                       >  1:387999/1‑250 (MQ=255)                    aGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGc                                                                                                                                                                                                                                <  1:179445/251‑1 (MQ=255)                             tCGGGATGCTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAATCCAGCGGTATAGt                                                                                                                                                                                                                        <  1:518964/250‑1 (MQ=255)                               gggATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATTAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGttat                                                                                                                                                                                                                     <  1:42575/251‑1 (MQ=255)                                 gATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGGATAGttatta                                                                                                                                                                                                                   <  2:584595/251‑1 (MQ=255)                                  aTGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACAAAGACCTAACAAACCAGCGGTATAGTtattat                                                                                                                                                                                                                  <  1:542993/251‑1 (MQ=255)                                             catcaATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTg                                                                                                                                                                                                        <  2:163380/250‑1 (MQ=255)                                                       agTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGtat                                                                                                                                                                                             >  2:284256/1‑251 (MQ=255)                                                           aGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAAcc                                                                                                                                                                                         >  1:198735/1‑251 (MQ=255)                                                               catcatTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTc                                                                                                                                                                                     >  1:455010/1‑251 (MQ=255)                                                                atcatTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCa                                                                                                                                                                                    <  1:272577/251‑1 (MQ=255)                                                                atcatTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCa                                                                                                                                                                                    <  2:174228/251‑1 (MQ=255)                                                                  catTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAg                                                                                                                                                                                   <  2:560051/250‑1 (MQ=255)                                                                   atTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATATGAATACCGGTATAACCCGTc                                                                                                                                                                                     >  1:429892/1‑247 (MQ=255)                                                                           cAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTc                                                                                                                                                                         >  1:98988/1‑251 (MQ=255)                                                                                 aaTTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGGCACATCGAAAGAAAATGCACCACCTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGa                                                                                                                                                                                                       <  1:61481/215‑1 (MQ=255)                                                                                 aaTTCTGCGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACAAGCGGTATAGTTATTATCGGTTATCTGa                                                                                                                                                                                                       >  2:61481/1‑215 (MQ=255)                                                                                      tgtgTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCgtagt                                                                                                                                                               >  1:178163/1‑250 (MQ=255)                                                                                              aTAGCGCTAGCCCGAGATATTCCGTGATGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAAATTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGACATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGCTAGTTCTTCAGGCCGTAGTAGTAgcttg                                                                                                                                                      >  2:57214/1‑251 (MQ=255)                                                                                                   gATAGGACGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATATCCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGa                                                                                                                                                 <  2:124075/251‑1 (MQ=255)                                                                                                      aGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGaaat                                                                                                                                              >  2:250323/1‑251 (MQ=255)                                                                                                             gATATTCAGTGAAGTACTTGTTTCTTCGAATAACTGGGTCCAGGAAACACGATAACTGTGCCCCTGGTAGGGTTTATCATCCGGGATACTAACAGGGGAATTAGTCACAGTGAAAGAAAAGGCACCAACTGAAGTAGTTAGACCAAGACCTAACAAACCAGCGGTATAGTGATGATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCGTGCTTGAAATAAATTAg                                                                                                                                       <  1:608120/251‑1 (MQ=255)                                                                                                               tatTCAGTGAAGCACTTGTTTCTTCGAATAACTTGTTACAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGATATTCAGGCCGTAGTAGTAGCTTGCTTGACATAAATTAGGc                                                                                                                                     >  2:319467/1‑251 (MQ=255)                                                                                                                atTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGc                                                                                                                                     <  1:482607/250‑1 (MQ=255)                                                                                                                       gAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTTTTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCt                                                                                                                              <  1:409516/250‑1 (MQ=255)                                                                                                                              ttGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGCAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACAAGCGGAATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATACATTAGGCTCATCATGCATatc                                                                                                                       >  2:28814/1‑250 (MQ=255)                                                                                                                               tGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCAGCCTGAATatca                                                                                                                      <  2:540930/250‑1 (MQ=255)                                                                                                                                      tcGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTa                                                                                                               <  2:455010/250‑1 (MQ=255)                                                                                                                                             aCTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTg                                                                                                        >  1:179825/1‑250 (MQ=255)                                                                                                                                                          aaCACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAatat                                                                                          >  2:8860/1‑251 (MQ=255)                                                                                                                                                             acGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATccc                                                                                       >  2:456362/1‑251 (MQ=255)                                                                                                                                                                            ctgttATGTTTTCTCATCCGGGATACGAACATTGGAATGAGTCCCATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGATTGCTTGAAATAAATTAGGCTCATCCTTAATATCATCTTTTAAGACCTGAACGCCGCAACTATACACACGTCCAACGcc                                                                          >  2:28319/5‑249 (MQ=255)                                                                                                                                                                              ggTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGCAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAggg                                                                      >  1:68797/1‑251 (MQ=255)                                                                                                                                                                                           atcCGGGATAAGAACATTGGAATGCGTCACATTGCAAGCAAATGCACCAAATGAAGTCTTTAGACCCAGACCTAACAAACCAGCGGTATAGTTCTTATCGGTTATCTGAATACCGGCATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCACCGCCAGGCCGTAACATTTGa                                                          >  2:598978/1‑250 (MQ=255)                                                                                                                                                                                                 gATACAAAAATTGAAATGAGTAACACCAAAAAAAAATGCCCAAACTGAAGTATTTAGACCAAGCCCAAACAAACCAGGGTTATATTTATTATCGGTTATCTGAATACCGGTAAAACCCGCAAGATAGTTATTCAGCCCGGAGTAGTAGCTTGCTTGAAATAAATTAGGCTCACCCTAAATATCACCTTTTAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACgg                                                   <  2:68797/251‑1 (MQ=255)                                                                                                                                                                                                              gAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTTCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTg                                      >  2:63924/1‑251 (MQ=255)                                                                                                                                                                                                                aTGAGTCACATCGAAAAAAAATGCACCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCGGAATCCCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTGGCTTGAAATAAATTAGGCTCACCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCg                                    <  2:98988/251‑1 (MQ=255)                                                                                                                                                                                                                         aTCGAAAGAAAATGCACCAACTGAAGCATTTAGACCAAACCCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGCATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGAAGAATCCTGAATATGATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCAAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGAGCGAATGTcc                           >  2:397722/1‑251 (MQ=255)                                                                                                                                                                                                                                        aCCAACTGAAGTATTTAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCgg            >  1:601246/1‑251 (MQ=255)                                                                                                                                                                                                                                                 aGTATTTAGACTAAGACCTAACAAACAAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCAGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTACAACGCCAGGGCGTAACATTTGAACCACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCg    >  2:404989/1‑250 (MQ=255)                                                                                                                                                                                                                                                  gTATTTAGACCAAGACCTAACAAACCAGCGGTATGTTTATTATCGGTGATCTGAAGACAGGTAAAACCCGTAAGATATTTATTCAGGCCGTAGTAGTAGCTTGTTTAAAATAAATACGGTTCATCCTGAATACCATCTTTAAAGCCCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAt  <  2:150560/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                      ACGCATGGATTTCGGTTCAAGATCTTGTTCGGGATGTTTCACTTCATCAATTAGAGTTAGTGCATCATTAAGACCAAGGTAATTCTGTGTCGAATAGCGATAGGCCGCGATATTCAGTGAAGTACTTGTTTCTTCGAATAACTTGTTCCAGGAAACACGATAACTTTGCCCCTGGTATGTTTTATCATCCGGGATACGAACATTGGAATGAGTCACATCGAAAGAAAATGCACCAACTGAAGTATTCAGACCAAGACCTAACAAACCAGCGGTATAGTTATTATCGGTTATCTGAATACCGGTATAACCCGTCAGATAGTTATTCAGGCCGTAGTAGTAGCTTGCTTGAAATAAATTAGGCTCATCCTGAATATCATCTTTTAAGACCTGACCGCCGCTAATATCCCAACGTCCAACGCCAGGGCGTAACATTTGAACAACGGATGAGAAAGGTTGCGAGAATGTCCGCTTTGAGCCATCGGATTCTTCGAT  >  NC_000913/153936‑154427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑