Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	46,661	T→C	N285N (AAT→AAC)	yaaU →	putative transporter YaaU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	46,661	0	T	C	98.0%	159.5 / NA	51	N285N (AAT→AAC)	yaaU	putative transporter YaaU
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (18/32);  minor base G (0/1);  total (18/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

AAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGAC  >  NC_000913/46414‑46900                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 acgggagAGTAAAAGAGTGCGAAAAGATGATGATAAAACTGTTTGGCGAAACGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCCGGTGATCCCAATGTTCGCCATTTACCCCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAAcgtg                                                                                                                                                                                                                                               >  2:547669/7‑251 (MQ=255)      cGAGTAAAAGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTGTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGAt                                                                                                                                                                                                                                          <  1:419761/251‑1 (MQ=255)        aGTAAAAGGGGGCGAAGAGATGATTATAAAACTGGTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAATCCCGGTTTCGCGATCTGGTTAATCGCCGCCATTTTCCGTTTGTTCTGGTTGGTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTGACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGAGAAGGTGGGGATTa                                                                                                                                                                                                                                        <  2:602140/251‑1 (MQ=255)             aaGAGTGCGAAGAGATGATGATAAAACTGTTTGGCGACCCGGTGGCTTTCGGTGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAAGCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTGGGCCCGCAAATCGTTGGGTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGTGGAACGTGGTGATTAGCCTg                                                                                                                                                                                                                                   <  2:43923/251‑1 (MQ=255)                agTGCGGAGAGATGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCCTCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGCTTGGGTGTTGGCAAAAACGCGGCGCTGGGGGACGTGGAGTTTAGCCTGTTc                                                                                                                                                                                                                                >  2:542893/1‑251 (MQ=255)                     gAAGAGATGATGATAACACTGTTTGGCGAACAGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTAt                                                                                                                                                                                                                           >  2:551162/1‑251 (MQ=255)                      aaGAGATGATGATAAAACTGTTGGGCGAACCGGTCGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGATTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACATGCCAGGTGATCCCAATGTTCGCCATTTTCACCTTTGGCCCGCAAATCGTTGTTTTGTTGGGTTTGGGTGTTGGCAAAAACGCGGCGCTGGGGCACGTGGTGATTAGCCTGTTCTTTATg                                                                                                                                                                                                                          >  1:481525/1‑251 (MQ=255)                         agaTGATGATAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGACCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGACATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTc                                                                                                                                                                                                                       >  1:602581/1‑251 (MQ=255)                          gatgatgatAAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCg                                                                                                                                                                                                                      <  1:154120/251‑1 (MQ=255)                                 ataaaaCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTAt                                                                                                                                                                                                               <  2:503494/248‑1 (MQ=255)                                 ataaaaCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGGTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCAGTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTAt                                                                                                                                                                                                               <  2:40527/248‑1 (MQ=255)                                   aaaaCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTc                                                                                                                                                                                                             <  1:96402/250‑1 (MQ=255)                                    aaaCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTcc                                                                                                                                                                                                            <  1:91498/251‑1 (MQ=255)                                      aCTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTccgc                                                                                                                                                                                                          <  1:10814/251‑1 (MQ=255)                                                     ggTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTa                                                                                                                                                                                            >  1:49796/1‑250 (MQ=255)                                                       tGGCTTTCGATGAAGAGCCGCAGCAGCACACCCGTTTTCGAGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAac                                                                                                                                                                                         >  2:120327/1‑251 (MQ=255)                                                                aTGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGa                                                                                                                                                                                <  2:346932/251‑1 (MQ=255)                                                                    agagCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGc                                                                                                                                                                            <  1:23315/251‑1 (MQ=255)                                                                          gcCGCAGCAAACCCGGTTTCGCTATCTGTTTAATCGCCGCCAGTTCCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTTAGCCCAATGTGCGCCATTTTCACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATGGGGTGTTGGCAAAAGCGCGGCGCTGGGGAACGTGTGGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCAt                                                                                                                                                                      <  2:33931/251‑1 (MQ=255)                                                                                    aCCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTgg                                                                                                                                                            >  1:66074/1‑251 (MQ=255)                                                                                        gTTTTCGTGATCTGATTAATCGCAGTCATTTTCCTTTGGTTCGGGTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAAAGCGGCGCTGGGGAACGTGGTGATTCGCCTGTTCTTTATGCTCGGCGGTATTCAGCCGATTCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCa                                                                                                                                                          <  1:177218/249‑1 (MQ=255)                                                                                           ttCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCTCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCGCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCtt                                                                                                                                                      <  2:66074/250‑1 (MQ=255)                                                                                                gATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTc                                                                                                                                                                                                                       <  1:234512/180‑1 (MQ=255)                                                                                                gATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTc                                                                                                                                                                                                                       >  2:234512/1‑180 (MQ=255)                                                                                                       ttAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcg                                                                                                                                         >  1:194278/1‑251 (MQ=255)                                                                                                           tCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctgg                                                                                                                                     >  1:128930/1‑251 (MQ=255)                                                                                                             gccgccATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcg                                                                                                                                   <  1:249439/251‑1 (MQ=255)                                                                                                               cgccATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCCGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgct                                                                                                                                 >  1:321288/1‑251 (MQ=255)                                                                                                                 ccATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGAcgctggcgctgg                                                                                                                               >  1:438735/1‑251 (MQ=255)                                                                                                                                  tgtttgttGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCACATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCtggtc                                                                                                             >  1:90491/1‑247 (MQ=255)                                                                                                                                       gttgCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGa                                                                                                        <  2:619052/251‑1 (MQ=255)                                                                                                                                         tgCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGat                                                                                                       <  1:146545/250‑1 (MQ=255)                                                                                                                                               cATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGGTATggggg                                                                                                >  1:130141/1‑250 (MQ=255)                                                                                                                                                    ggACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGAGCACGGATATGGGGATctg                                                                                            <  2:294983/250‑1 (MQ=255)                                                                                                                                                    ggACCTGCCAGGTGATCCCAATGTTCGCCATTGACACCTGTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGTCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCGTTGCCATGAGGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGAt                                                                                               <  1:379163/247‑1 (MQ=255)                                                                                                                                                    ggACCTGCCACGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGACCGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGGGTTTTGGGG‑TGTTTCCGGCTATGGGGATctgg                                                                                           >  2:109585/1‑251 (MQ=255)                                                                                                                                                     gACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTTGGGCTGTTTCCGGGTATGGGGagttggg                                                                                          >  2:443915/1‑245 (MQ=255)                                                                                                                                                          gCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATctggctgg                                                                                       <  1:29169/249‑1 (MQ=255)                                                                                                                                                                  aTCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATTGGGGTCTGGCTGGTGGTGATGGc                                                                             >  1:397168/1‑251 (MQ=255)                                                                                                                                                                     ccAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCtt                                                                          <  1:443915/251‑1 (MQ=255)                                                                                                                                                                        aTGTTCGACATTTACACATATGGCCCGCACATCGTTGGTTTGTTGGGATTGGTTGCTCTCAAAAACGCGGCGCTGGGGACCGGGGTGATTAGCATGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGAAGCTTTGCCCTGCTGACGCTGGCGATGGCGGGTTGTGGGG‑TGATCCCCGCTATTGGGGTCTGGACGGTAGGGGTGGCCTTTGc                                                                       >  2:42081/1‑251 (MQ=255)                                                                                                                                                                          ggtCGCCATTTACACCTTTGGCCCGCAAATCTTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCg                                                                      <  2:552472/248‑1 (MQ=255)                                                                                                                                                                            tCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGTCCTTTGCGgt                                                                    <  1:485942/250‑1 (MQ=255)                                                                                                                                                                              gCCATTTACACCTTTGGCCCGCAAATCTTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGAGCCCGGATATGGGGATCTGGCTGGTAGTGATGGTCTTTGCGGTGTa                                                                 <  2:311653/251‑1 (MQ=255)                                                                                                                                                                              gCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTGTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGTGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTa                                                                 <  1:551162/251‑1 (MQ=255)                                                                                                                                                                                 aTTTACGCCTTTGGCCCGCAAATCGTTTGTTTGTGGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGACCGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTTGTTAAACTCTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTGGGGGCTGATCCCGGATATGTGGATCTGGCTGGTAGTGATGGCCTTTGCGATGTATGc                                                              <  2:462969/251‑1 (MQ=255)                                                                                                                                                                                   ttACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGTAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTTGCTGGTAGTGATGGCCTTTGCGGTGTATGcc                                                             <  1:547669/250‑1 (MQ=255)                                                                                                                                                                                           ttGGACCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCGTTATGCTCGGCTGTATGCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑GTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGTCCTTTGCGGTGTATGCCTTTTtct                                                      <  2:321288/249‑1 (MQ=255)                                                                                                                                                                                             ggCCCGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTgg                                                  <  1:109585/251‑1 (MQ=255)                                                                                                                                                                                                 cGCAAATCGTTGGTTTGTTGGGATTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCggg                                              <  1:354732/251‑1 (MQ=255)                                                                                                                                                                                                                       aTTGGGTGTTGGCAAAAACGCGGCGCTGGGGAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGctct                        <  1:507941/251‑1 (MQ=255)                                                                                                                                                                                                                                             gcgctggggAACGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCATCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTGATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATGTGCAGTGGCTCTATCCTAATGAACTCTTCCCGAc  <  1:120327/245‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                 AAGGGCGAGTAAAAGAGTGCGAAGAGATGATGATCAAACTGTTTGGCGAACCGGTGGCTTTCGATGAAGAGCAGCCGCAGCAAACCCGTTTTCGCGATCTGTTTAATCGCCGCCATTTTCCTTTTGTTCTGTTTGTTGCCGCCATCTGGACCTGCCAGGTGATCCCAATGTTCGCCATTTACACCTTTGGCCCGCAAATCGTTGGTTTGTTGGGATTGGGGGTTGGCAAAAACGCGGCACTAGGGAATGTGGTGATTAGCCTGTTCTTTATGCTCGGCTGTATTCCGCCGATGCTGTGGTTAAACACTGCCGGACGGCGTCCATTGTTGATTGGCAGCTTTGCCATGATGACGCTGGCGCTGGCGG‑TTTTGGGGCTAATCCCGGATATGGGGATCTGGCTGGTAGTGATGGCCTTTGCGGTGTATGCCTTTTTCTCTGGCGGGCCGGGTAATTTGCAGTGGCTCTATCCTAATGAACTCTTCCCGAC  >  NC_000913/46414‑46900

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑