| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 26,624 | T→G | S116S (TCT→TCG) | ispH → | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 26,624 | 0 | T | G | 99.0% | 309.4 / NA | 97 | S116S (TCT→TCG) | ispH | 4‑hydroxy‑3‑methylbut‑2‑enyl diphosphate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (45/51); total (45/52) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCAC‑CCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGAC > NC_000913/26387‑26861 | cGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGCGCGTGGGGCTATCTTTCTTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCggcc > 2:253386/1‑249 (MQ=255)cGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTAACGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCggcc > 2:237568/1‑249 (MQ=255) gAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCggcc > 1:176370/1‑248 (MQ=255) aGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACg < 2:86501/251‑1 (MQ=255) gTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGc > 2:506690/1‑251 (MQ=255) gTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCgg > 1:582958/1‑251 (MQ=255) gTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGGGGCGAAGAGTCGATTCTCATCGGCCACGCCgg > 2:498471/1‑251 (MQ=255) aTAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCat < 1:460493/251‑2 (MQ=255) aaCCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGAGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCatcc > 2:21056/1‑248 (MQ=255) atATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGAGTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGGAT‑CCGGAAg < 1:115067/250‑1 (MQ=255) tATGTGGTCGATAGCTTGCGTGGGCGTGGCGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGCTCCTGATTTTCTCAGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGAAAAAAGCCGCGATTTGACGGTGTTTGATGCCCCCTGTCCGCTGGCGACCAAAGTGCATATGGATGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCggggatcaggaagg > 1:293936/1‑240 (MQ=255) aTGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTg < 1:34968/251‑1 (MQ=255) aTGTGGTCGATAGCTGGCGTGAGCGTGGGGCTATCTGTATTGAGCAGAGTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTg < 2:629700/251‑1 (MQ=255) gtgGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTgg > 2:572728/1‑250 (MQ=255) gATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAggtac > 2:270112/1‑248 (MQ=255) aTAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAggtacg > 1:508426/1‑247 (MQ=255) aGCTTGCGTGAGAGTGAAGCTATATTTCTTGAGCAGATTAGAGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGAACACGGAGATTCACAGGCGGCACGTAACGAAGCAAAAAGCCGCTATTTGACGGTGTTTGATGACACCTGTCCGACGGTGACCACAGCGCATATGGAAGCCGCCCGCGCAAGCCGTCGTGGCGCAGAGTCGATTCTCCTCGGCCACGCCGGGCAT‑CCGGAAGTGGAAggtacga > 2:617458/1‑245 (MQ=255) tGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGc > 1:75255/1‑251 (MQ=255) gttaGCGTGTGGCTATCTTTATTGATCAGATTAGCGAAGTACCGGACGGCGCGTTCCTGATTTGCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCATAAAGCCGCTATTTGACTGTGTTTGATGCCACCTGGCCGCTGGTGACTAAAGGGCATATGGAAGTCGCCCGCGACAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAATGTACGATGGGCcag < 1:410459/248‑1 (MQ=255) gTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGcc > 1:75786/1‑249 (MQ=255) gCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTCTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCcagtaca < 2:168704/251‑1 (MQ=255) gTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATCTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCcattacagt < 2:561412/251‑1 (MQ=255) ggggCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCcagtacagta < 2:176370/250‑1 (MQ=255) tATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCGCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAggt < 2:589743/250‑2 (MQ=255) aTTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAgggg < 1:261965/250‑1 (MQ=255) aTTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGGGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAggggg < 2:260500/251‑1 (MQ=255) tGAGCAGATTATCGAGGTACCGGACGGCGCGCTCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGACGTGGAAGGCACGATGGGACAGTACAGTAACCCGGCAGGGGGa > 2:28162/1‑250 (MQ=255) tGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGa > 1:469347/1‑250 (MQ=255) gATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGACGGGGGAATGTATc > 1:11428/1‑251 (MQ=255) gATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATc > 2:326541/1‑251 (MQ=255) tAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTgg > 1:93581/1‑251 (MQ=255) tACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGGGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCg > 2:180811/1‑251 (MQ=255) ccGGACGGCGCGATCCTGATGTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACTGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAGCCCGGAAGGGGGAATGTATCTGGTCGAATCGcc < 2:560261/251‑1 (MQ=255) gcGGCGCGATCCTGATTTTCGCCGCGCACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGAc < 2:172429/250‑1 (MQ=255) gggctctagcttgaatttCTCCGCACTCGGTGTTGCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGAGTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTGGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGTGCAT‑CCGGAAGTTGAAGGGGCGATGGGCCAGTACAGTAACCCGGAAGGGGGATTGTATCTGGTCGAATCGCCGGAc < 2:359436/235‑1 (MQ=255) ggCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGG‑ACTCCGGCAGTGGAAGGAACGATGGGCCAGTACAGTAACCCGGCAGGGGGATGGTAGCTGGTCGAATCGCCgggcgg > 2:482054/1‑247 (MQ=255) ggCGCGATCCTGATTTTCTCCGCACACGGTGTTTCGCAGGCGGGACGTACCGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCGGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAGGTGGAAGGTACGATGGGCCAGTACAGTACCCCGGAAGGGAGAGTGTATCTGGTCGAATCGCCGTACg > 2:453311/1‑250 (MQ=255) gcgcGATCCTGATTTTCTCCGCACACGGTGTTTCGCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTGGACGGTGTTTGATGCCACCTGTCCTCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGGACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAt < 1:378132/251‑1 (MQ=255) gATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTCTCTGGTCGAATCGCCGGACGAtgtgg > 2:469112/1‑250 (MQ=255) aTCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTTCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGAtgtgtg < 1:419724/251‑1 (MQ=255) tCCTGCTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAATGAAGCAAAACGCCGCGATTTGACGGTGTTTGCTGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTAATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGACGAATCGCCGGACGATGGGTgg > 2:34207/1‑251 (MQ=255) actttCTCCGCCCTCGGTGTTTCCCAGGCGGTACGTAACGAAGTAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTCTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGGCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACt < 1:326478/249‑1 (MQ=255) aTTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACt < 1:449393/251‑1 (MQ=255) tttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTg > 2:221632/1‑250 (MQ=255) tttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGGATCTGGTCGAATCGCCGGACGATGTGTGGAAACTg < 2:419454/250‑1 (MQ=255) ttCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGa < 2:394460/250‑1 (MQ=255) gtctcCGCACCCGGTGGTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCTCGCCAGCCGTCGTGGCGAAGAGTCTATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGTAATGTATCTGGTCGAATCGCCGGACTATGTGTGGAAACTGa < 1:184564/249‑1 (MQ=255) gcccacGGGGTTTCTCAGGTGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGCCCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGttaa < 2:341162/248‑4 (MQ=255) ccacGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCaaaa < 1:92411/250‑1 (MQ=255) acacGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCaaa > 1:64994/1‑250 (MQ=255) ccGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTGTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAAc < 2:246376/250‑1 (MQ=255) gtgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGTCGGTCAAAAACGaa < 2:423669/251‑1 (MQ=255) gtgtTTCTCAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGaa < 1:221632/251‑1 (MQ=255) ctcAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAgaga > 1:99679/1‑249 (MQ=255) cAGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAATTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTGCAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGct < 2:476443/251‑1 (MQ=255) aGGCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGctc > 1:264817/1‑251 (MQ=255) ggCGGTACGTAACGAAGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGGCCGCTGGTGACCAAAGGGCATATGGAAGGCGCCCGCGCCAGCCGGCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGctct < 2:233922/251‑1 (MQ=255) aCGTAACGAAGCAAAAAGCCGCGATCTGACGGTGGTTGATGCCACCTGGCTGCTGGTGACCAAAGTTCAGATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAACCGAAGAGAAGCTCTCCTTTa < 2:312072/251‑1 (MQ=255) gTAACTAAGCAAAAAGTCGTGATTTTACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCTGGGAAT‑CCGGAAGTGGAAGGTTCGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATg < 1:297568/251‑1 (MQ=255) gaatcAAAAAGCCGCGCTTTGACGGTGTTTGCTGCCACCTGTCCGATGGTGACCAAAGTGCATATGGAAGCCGCCCGCGCCAGCCGTCGCGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGCAGTGGAAGGTAAGATGGGCCAGTCCAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCACCTTTATGAccc > 2:409491/5‑250 (MQ=255) aaGCAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAg < 1:50447/251‑1 (MQ=255) cAAAAAGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGAcc > 1:206118/1‑251 (MQ=255) aaaGCCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACg < 1:539843/251‑1 (MQ=255) gccgcgATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGCTGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACTATGTGTGTAAACTGACGGTCAAAACCGAAGAGCAGCTCTCCTTTATGACCCAGACCCCGCtt > 1:381081/3‑250 (MQ=255) gccgcgATTTGACGGTGATTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATAGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTg > 1:510574/3‑251 (MQ=255) aCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGa < 1:245721/251‑1 (MQ=255) aCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCAGCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGa < 1:253386/251‑1 (MQ=255) aCGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGAGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGTCGATGTGTGGAAACTGACGGTCAAAAAAGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATg > 2:515922/1‑250 (MQ=255) aTGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAt < 2:592773/251‑1 (MQ=255) tGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGCAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtg > 2:468315/1‑251 (MQ=255) gCCACCTGGCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtg < 2:124396/250‑1 (MQ=255) ccACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTCTCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGAtgtg < 1:469112/251‑1 (MQ=255) tGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCg > 1:591517/1‑250 (MQ=255) gggccgcgggggACCAAAGTGCATAGGGAAGTCGGCGGCGACAGCCGTCGGGGCGAAGAGTCGATGCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGTGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCGCCTTTATGACCAAGACCACGCTGTCGGTGGATGAAACGTCTGATGTGAttga < 2:381081/240‑4 (MQ=255) gTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGGCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCg < 1:267822/249‑1 (MQ=255) ccGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGAcgc > 1:488112/1‑251 (MQ=255) cGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGGGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACGCGTCTGATGTGATCGAcgcg < 1:498471/251‑1 (MQ=255) cGCTGGGGGCCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACGGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATTACACGTCGGATGTGATCGAcgcg < 2:558479/251‑1 (MQ=255) gCTGGCGACCAAAGAGAATATGGAAGTCGCCCGCGCTAGCCGTCGTGACGAAGAGTCGATTATCACCGCCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGACGGGCCCGTACAGTAACCCGGAAGGGGGAATGTATCTGGACGACTCGCCGGCCGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGGGGATGCCCCGTGTGATGTGATCGCCgcgc > 2:325836/1‑251 (MQ=255) cTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGAACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACgcgcc > 2:237431/1‑250 (MQ=255) tGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGt > 1:426530/1‑251 (MQ=255) ccAAAGTGCATCTGGCAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGCTGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTaa > 2:360931/1‑250 (MQ=255) cAAAGTGCATATTGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCTCGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGTTGCGTAAAc < 2:439794/251‑1 (MQ=255) cAAAGTGCATATGGAAGTCGCCCGCGCCAGCCGGCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACTATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCCGACCACGCTGTCGGTGGATGACACGTCTGATGTGAGCGACGCGCTGCTTAAAc < 2:272651/251‑1 (MQ=255) aaGTGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CAGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGc > 1:317570/1‑251 (MQ=255) tGCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGAGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGCTGTGATCGCCGCGCTGCGTAAACGCTTc > 1:388165/1‑251 (MQ=255) gCATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTcc > 2:284590/1‑251 (MQ=255) cATATGGAAGTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCTACGCGCTGCGTAAACGCTTccc < 1:470964/251‑1 (MQ=255) ggTCGCCCGCGCCAGCCGTCGTGGCGAAGAGTCGAGTCTCCCCTGCCACGCCGGGCAT‑CCGTCAGTGGAAGGTACGATTGGCCAGTACAGTAACCCGGAAGGTGGAATGTATCTGGTCGAATCGCCTGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCTGTGGATCACACGTCTGATGTGATCGACGCTCTGCGTAAACGCCTCCCGAAAATTg < 2:22396/250‑1 (MQ=255) gCCCGCGCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCgg < 2:75786/251‑1 (MQ=255) gCCCGCGCCAGCCGTCGTGGCGAACAGTCGATTCTCATCGGCCACGCCGAGCAT‑CCGGAAGTGGAAGGTACGCTGGGCCCGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTATGATGTGATCGCCGCGCTGCGTAAACGCTTCCCGAAAATTGTCgg > 2:328548/1‑251 (MQ=255) cgcgCCAGCCGTCGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCGGCGTAAACGCTTCCCGAAAATTGTCGGTcc < 1:180811/251‑1 (MQ=255) ccgtcGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAg < 1:67299/247‑1 (MQ=255) gtcGTGGCGAAGAGGCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGAt < 1:518544/249‑1 (MQ=255) cGTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATg < 1:24248/250‑1 (MQ=255) gTGGCGAAGAGTCGATTCTCATCGGCCACGCCGGGCAT‑CCGGAAGTGGAAGGTACGATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGAc > 2:86986/1‑251 (MQ=255) gTGGCGAAGAGGCGATTCTCACCGGCCACGCCGGGCAT‑CCAGAAGTGGAAGGTACGATGGGCCAGTACAGTAGCCCCGAAGGGAGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACACAGACCGCGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGa < 2:15222/250‑1 (MQ=255) | CGAAGTGGTACATAACCGCTATGTGGTCGATAGCTTGCGTGAGCGTGGGGCTATCTTTATTGAGCAGATTAGCGAAGTACCGGACGGCGCGATCCTGATTTTCTCCGCACACGGTGTTTCTCAGGCGGTACGTAACGAAGCAAAAAGTCGCGATTTGACGGTGTTTGATGCCACCTGTCCGCTGGTGACCAAAGTGCATATGGAAGTCGCCCGCGCCAGTCGCCGTGGCGAAGAATCTATTCTCATCGGTCACGCCGGGCAC‑CCGGAAGTGGAAGGGACAATGGGCCAGTACAGTAACCCGGAAGGGGGAATGTATCTGGTCGAATCGCCGGACGATGTGTGGAAACTGACGGTCAAAAACGAAGAGAAGCTCTCCTTTATGACCCAGACCACGCTGTCGGTGGATGACACGTCTGATGTGATCGACGCGCTGCGTAAACGCTTCCCGAAAATTGTCGGTCCGCGCAAAGATGAC > NC_000913/26387‑26861 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |