Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,685	G→A	R160K (AGA→AAA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,685	0	G	A	100.0%	119.5 / NA	38	R160K (AGA→AAA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/21);  total (17/21)

GTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAA  >  NC_000913/25439‑25923                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               gTATTAGCGTGATCCTGGCAGTGATGATGGATCGCTCGAAGGCAACGCAGAACCTAAACAATTTCGCTTCCGGGCTGATTTTTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCTACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGAAGGTTTTTTACCTTCTAAAGCg                                                                                                                                                                                                                                            <  1:478382/251‑1 (MQ=255)     tAGCGTGATCCTGGGAGTGAGGATGTCTCGCTCGAAGGCCCCGCAGAAGCTAAAAAATTTCGCTAACGCTCGTATTATTGGCGGCGCGTTGGGCACCC‑TGTTCGTCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaaa                                                                                                                                                                                                                                         <  1:226123/250‑1 (MQ=255)      aGCGTGATCCTGGATGTGGTGATGTGCCTCGCGAAGGCCACGCAGAAGCGAAACAATTTCCCTTCCGCCCTGAATATTGCCGCCGCGCGGGGAAGCT‑TGTTCGACCGCCTGTGGCACGGCTGCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACGTCGCCACCTTCAAGCTTGCCGATACTGCCATCTGTGTCGGTTCGGCTCTGATTGTGCGGGAAGGTTTTTTACCCTCTAAAGCGaaaa                                                                                                                                                                                                                                        <  2:376144/250‑1 (MQ=255)         gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACATTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACa                                                                                                                                                                                                                                    <  1:467823/251‑1 (MQ=255)            aTCCTGGCGGGGATGTTTTATCGCCCGAGGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGTTTATTGGCGGCGCGCTGGG‑AACCGTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaata                                                                                                                                                                                                                                 <  1:435209/251‑1 (MQ=255)                                  gCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatg                                                                                                                                                                                                           <  1:491848/251‑1 (MQ=255)                                  gCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACAGCCTGTGGCCCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatg                                                                                                                                                                                                           >  1:291729/1‑251 (MQ=255)                                     cgaggcccccgaaGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGTGGGGCACCC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTg                                                                                                                                                                                                        <  2:495115/239‑1 (MQ=255)                                     cGAAGGCCACGCAGAAGCTAAACAATATCGCTTCCGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTg                                                                                                                                                                                                        >  1:136745/1‑251 (MQ=255)                                          gCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGc                                                                                                                                                                                                    >  1:421298/1‑250 (MQ=255)                                           ccACGCAGAAGAGAAACAATTTCGCTTACGCTTTGTTGTTTGGCGGCGCGCTGGGACCCC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAAGAAACCCTTCCGGATGCGATTCTGACGCAt                                                                                                                                                                                                  <  1:433537/251‑1 (MQ=255)                                                     gCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGc                                                                                                                                                                                        >  1:549147/1‑251 (MQ=255)                                                                              attattGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTATAAAGCGAAAAAACAATAATAAACCCTGCCGGAGGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAgg                                                                                                                                                               >  1:331980/1‑251 (MQ=255)                                                                                tattGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGCAGGcc                                                                                                                                                             >  1:214422/1‑251 (MQ=255)                                                                                             cTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATCTGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGCCGCATCTTATCCGGCCTACAGATTGCTGCGACATCGTAGGCCGGATAAGGCGttt                                                                                                                                                >  2:311893/1‑251 (MQ=255)                                                                                                   cccc‑TGTTCGCCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  2:349496/249‑1 (MQ=255)                                                                                                    ccc‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGAGCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTTCCGATACTGCCATCTGTGTCGTTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTTTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTTACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                          <  1:54084/249‑1 (MQ=255)                                                                                                       cgtttcaaCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                                                                       <  2:176641/244‑1 (MQ=255)                                                                                                           ccgtccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCGTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAAGCGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                                    <  2:313576/246‑1 (MQ=255)                                                                                                            cgtccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                  <  2:113920/248‑1 (MQ=255)                                                                                                                          aCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAat                                                                                                                    <  2:88903/251‑1 (MQ=255)                                                                                                                            ggCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                                  >  1:224819/1‑251 (MQ=255)                                                                                                                             gCTTCGTTGTCGATATGATAGACTTCTACGTCGGCGGATAGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGAGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTCTCCGGCCTACAGATTGCTGCGAGATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAAACACTCCTTAAatat                                                                                                                  >  1:378968/1‑250 (MQ=255)                                                                                                                                  gTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGc                                                                                                            <  1:536006/251‑1 (MQ=255)                                                                                                                                       cGATATGATCGACTTCTACGTCGGCTGCTGGCACTTCGCCACCTGCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc                                                                                                       <  1:315455/251‑1 (MQ=255)                                                                                                                                       cGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc                                                                                                       <  2:127950/251‑1 (MQ=255)                                                                                                                                                                         ttCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGCATCTGTACAGAGCAATAGCGCCg                                                                     >  2:5170/1‑251 (MQ=255)                                                                                                                                                                                   tCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAACTCGTAGGCCGGATAAGGCGTTTACGCCGCTTCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAAAGAGCAATAGCGCCGGCCTGATGc                                                            >  2:178461/1‑250 (MQ=255)                                                                                                                                                                                   tCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGc                                                            >  1:11269/1‑250 (MQ=255)                                                                                                                                                                                                 acttccATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAAGAGCGCCGTCCTGGTGCACTTCACGCTAAAAc                                             <  2:402270/247‑1 (MQ=255)                                                                                                                                                                                                      cATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCACCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgac                                        <  2:224819/251‑1 (MQ=255)                                                                                                                                                                                                          tgtgTAGGTGAGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTCAAGCGAAACAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATg                                    >  2:590748/1‑251 (MQ=255)                                                                                                                                                                                                            tgtCGGTGCGGCACTGATTGTGCTTGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCCGCCGGATGCGATGCTTACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAAAAATCCCTAAATATAAGAGCAAACCTGCATGTCTGAAACTGTACAAAGCAATAGCGCCCGCCTGGTGCACTTCACGCTAAAACTCGACGATgg                                   <  1:501847/250‑1 (MQ=255)                                                                                                                                                                                                             gtCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGc                                  >  2:178177/1‑250 (MQ=255)                                                                                                                                                                                                               cGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacc                               <  1:252746/251‑1 (MQ=255)                                                                                                                                                                                                                       cACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGACTCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCAccgccgc                       >  2:150338/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaatgTTTTTTACCTTCTAAAGCGAAAAAACAATAATACACCCTGCCGGATGCGATGCTGCCGCATCTTATCCGGCCTAAAGATTGCTGCGAAATCGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGATCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGAAGGCACAACCGCCGAGTCTACCCGccac          >  2:331055/5‑247 (MQ=255)                                                                                                                                                                                                                                             tttaccTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTaa  >  1:134648/5‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                               GTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAA  >  NC_000913/25439‑25923

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑