Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,677	G→A	L157L (TTG→TTA)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,677	0	G	A	100.0%	115.8 / NA	38	L157L (TTG→TTA)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (16/22);  total (16/22)

TGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAAC  >  NC_000913/25437‑25915                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               gggTATTAGCGTGATCCTGGCAGTGATGAGGTAGCGCTCGAAGGCCGCGCAGAAGCTAAACAATTTCGCTTACGCGGTGATTTTTGGCGGCGCGCGGGGCACCC‑TTTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaaag                                                                                                                                                                                                                                      <  2:547854/250‑4 (MQ=255)   gTATTAGCGTGATCCTGGCAGTGATGATGGATCGCTCGAAGGCAACGCAGAACCTAAACAATTTCGCTTCCGGGCTGATTTTTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCTACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGAAGGTTTTTTACCTTCTAAAGCg                                                                                                                                                                                                                                    <  1:478382/251‑1 (MQ=255)       tAGCGTGATCCTGGGAGTGAGGATGTCTCGCTCGAAGGCCCCGCAGAAGCTAAAAAATTTCGCTAACGCTCGTATTATTGGCGGCGCGTTGGGCACCC‑TGTTCGTCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaaa                                                                                                                                                                                                                                 <  1:226123/250‑1 (MQ=255)        aGCGTGATCCTGGATGTGGTGATGTGCCTCGCGAAGGCCACGCAGAAGCGAAACAATTTCCCTTCCGCCCTGAATATTGCCGCCGCGCGGGGAAGCT‑TGTTCGACCGCCTGTGGCACGGCTGCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACGTCGCCACCTTCAAGCTTGCCGATACTGCCATCTGTGTCGGTTCGGCTCTGATTGTGCGGGAAGGTTTTTTACCCTCTAAAGCGaaaa                                                                                                                                                                                                                                <  2:376144/250‑1 (MQ=255)           gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACATTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACa                                                                                                                                                                                                                            <  1:467823/251‑1 (MQ=255)              aTCCTGGCGGGGATGTTTTATCGCCCGAGGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGTTTATTGGCGGCGCGCTGGG‑AACCGTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaata                                                                                                                                                                                                                         <  1:435209/251‑1 (MQ=255)                                    gCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatg                                                                                                                                                                                                   <  1:491848/251‑1 (MQ=255)                                    gCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACAGCCTGTGGCCCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatg                                                                                                                                                                                                   >  1:291729/1‑251 (MQ=255)                                       cgaggcccccgaaGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGTGGGGCACCC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTg                                                                                                                                                                                                <  2:495115/239‑1 (MQ=255)                                       cGAAGGCCACGCAGAAGCTAAACAATATCGCTTCCGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTg                                                                                                                                                                                                >  1:136745/1‑251 (MQ=255)                                            gCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGc                                                                                                                                                                                            >  1:421298/1‑250 (MQ=255)                                             ccACGCAGAAGAGAAACAATTTCGCTTACGCTTTGTTGTTTGGCGGCGCGCTGGGACCCC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAAGAAACCCTTCCGGATGCGATTCTGACGCAt                                                                                                                                                                                          <  1:433537/251‑1 (MQ=255)                                                       gCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGc                                                                                                                                                                                >  1:549147/1‑251 (MQ=255)                                                                                attattGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTATAAAGCGAAAAAACAATAATAAACCCTGCCGGAGGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAgg                                                                                                                                                       >  1:331980/1‑251 (MQ=255)                                                                                  tattGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGCAGGcc                                                                                                                                                     >  1:214422/1‑251 (MQ=255)                                                                                               cTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATCTGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGCCGCATCTTATCCGGCCTACAGATTGCTGCGACATCGTAGGCCGGATAAGGCGttt                                                                                                                                        >  2:311893/1‑251 (MQ=255)                                                                                                     cccc‑TGTTCGCCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                  <  2:349496/249‑1 (MQ=255)                                                                                                      ccc‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGAGCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTTCCGATACTGCCATCTGTGTCGTTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTTTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTTACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                  <  1:54084/249‑1 (MQ=255)                                                                                                         cgtttcaaCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                                                                                                               <  2:176641/244‑1 (MQ=255)                                                                                                             ccgtccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCGTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAAGCGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                                                                                                                            <  2:313576/246‑1 (MQ=255)                                                                                                              cgtccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                          <  2:113920/248‑1 (MQ=255)                                                                                                                            aCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAat                                                                                                            <  2:88903/251‑1 (MQ=255)                                                                                                                              ggCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat                                                                                                          >  1:224819/1‑251 (MQ=255)                                                                                                                               gCTTCGTTGTCGATATGATAGACTTCTACGTCGGCGGATAGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGAGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTCTCCGGCCTACAGATTGCTGCGAGATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAAACACTCCTTAAatat                                                                                                          >  1:378968/1‑250 (MQ=255)                                                                                                                                    gTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGc                                                                                                    <  1:536006/251‑1 (MQ=255)                                                                                                                                         cGATATGATCGACTTCTACGTCGGCTGCTGGCACTTCGCCACCTGCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc                                                                                               <  1:315455/251‑1 (MQ=255)                                                                                                                                         cGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc                                                                                               <  2:127950/251‑1 (MQ=255)                                                                                                                                                                           ttCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGCATCTGTACAGAGCAATAGCGCCg                                                             >  2:5170/1‑251 (MQ=255)                                                                                                                                                                                     tCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAACTCGTAGGCCGGATAAGGCGTTTACGCCGCTTCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTAAAGAGCAATAGCGCCGGCCTGATGc                                                    >  2:178461/1‑250 (MQ=255)                                                                                                                                                                                     tCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGc                                                    >  1:11269/1‑250 (MQ=255)                                                                                                                                                                                                   acttccATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAAGAGCGCCGTCCTGGTGCACTTCACGCTAAAAc                                     <  2:402270/247‑1 (MQ=255)                                                                                                                                                                                                        cATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCACCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTcgac                                <  2:224819/251‑1 (MQ=255)                                                                                                                                                                                                            tgtgTAGGTGAGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTCAAGCGAAACAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATg                            >  2:590748/1‑251 (MQ=255)                                                                                                                                                                                                              tgtCGGTGCGGCACTGATTGTGCTTGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCCGCCGGATGCGATGCTTACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGGCAAAAATCCCTAAATATAAGAGCAAACCTGCATGTCTGAAACTGTACAAAGCAATAGCGCCCGCCTGGTGCACTTCACGCTAAAACTCGACGATgg                           <  1:501847/250‑1 (MQ=255)                                                                                                                                                                                                               gtCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGc                          >  2:178177/1‑250 (MQ=255)                                                                                                                                                                                                                 cGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGACGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGcacc                       <  1:252746/251‑1 (MQ=255)                                                                                                                                                                                                                         cACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGACTCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCAccgccgc               >  2:150338/1‑250 (MQ=255)                                                                                                                                                                                                                                       aaatgTTTTTTACCTTCTAAAGCGAAAAAACAATAATACACCCTGCCGGATGCGATGCTGCCGCATCTTATCCGGCCTAAAGATTGCTGCGAAATCGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGATCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGAAGGCACAACCGCCGAGTCTACCCGccac  >  2:331055/5‑247 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                               TGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAATATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAAC  >  NC_000913/25437‑25915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑