Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,598	A→G	D131G (GAC→GGC)	lspA →	lipoprotein signal peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,598	0	A	G	100.0%	116.4 / NA	40	D131G (GAC→GGC)	lspA	lipoprotein signal peptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/27);  total (13/27)

CATTATGCGCGTAACTATGGCGCGGCGTTTAGTTTCC‑TTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACC  >  NC_000913/25351‑25822                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                 cATTATGCGCGTAACTATGGCGCGGCGTTTAGTTTCC‑TTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGAGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACCTCGGCGGCTg                                                                                                                                                                                                                               >  1:156328/1‑251 (MQ=255)      ggcgcTTAACTATGGCGTGGCGTTT‑GTTTCCCTTGCCGATAGCGGGGGCTGGCAGCGTGGGTTCTTGG‑CCGTTATTGCGTTTGGTATTGGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCAACGCAGAAGCAAAACAAT‑TTCGCTTACGCGCTGATTATGGGCGGCGCGTTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACt                                                                                                                                                                                                                          <  1:265897/250‑1 (MQ=255)            taccttgggcgcgtggTTTAGTTTCC‑TTGCGGTTGGCGGCGGCTGGCGGCGTTGGTTTTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCGGGCAGTGATGAGGTATCGCTCGAAGGCCACGCAGAGGCTAAACAAT‑ATCGCTTCCGGGCTGATTATGGGCGGCGTGCTGGGCAACC‑TTTTGGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGcc                                                                                                                                                                                                                     <  1:284736/235‑1 (MQ=255)                   ggCGCGGCGTTTAGTTTCC‑TTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCa                                                                                                                                                                                                              >  1:399991/1‑250 (MQ=255)                        ggcgTTTAGTTTCC‑TTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTg                                                                                                                                                                                                        >  1:414260/1‑251 (MQ=255)                              ttgttttctttgcggtgggcggggccTGGCAGGGTTG‑TTGTTTGGCCGGTATTGCGATTGGTATAAGCTTGATGCTGGCAGTGATGATGGAGCGCCCGAAGGCCACGCAGAAGCTAAACAAT‑ATCTCTTGCGCGCGGATTATTGGCGGCTCGCGGGGCAACC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCTACTTCTACGGCGGCGGCTGGCACTTCGCCACCTGCAACCTTGCCGa                                                                                                                                                                                                    <  2:598609/225‑1 (MQ=255)                               aGTTTCC‑TTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTG‑CCGGTATTTCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCCCCTTCAACCTTGCCggta                                                                                                                                                                                                  >  2:528319/1‑247 (MQ=255)                                         gCCGATAGCGGCGGCTGGAAGCGTTGGTTCTTTG‑CGGTTATTGGGATTGGTATTAGCGTGATCCTGGAAGTGATGATGTAGCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑TTCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtg                                                                                                                                                                                        <  1:197170/251‑1 (MQ=255)                                           cGATTGCGGCGGGTGGTAGTGTTGGTTCTGTG‑CCGGGATTGCGAGTTGTATTAGCGTGATCCTGGCAGTGATTATGTATCGCTGGAAGCCCGCTCAGAAGCTAAACAAT‑ATGTCTTACGGGCTGGTTATTGGCGGCGCGCTGGGCAACC‑TGTTCGGCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACTTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCtgtg                                                                                                                                                                                      <  1:102030/251‑1 (MQ=255)                                                      gctggtagcttgggTTCTTTG‑CGGGTATTGCGATTGGTATTAGCGTGATCCGGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATGGGCGGCGCGCTGGGCAACT‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCGTGCCGATGCTGCCATCTGTGTCGGTGCGGCa                                                                                                                                                                           <  1:140095/239‑1 (MQ=255)                                                              ggTTGGTTCTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCACCC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATtgt                                                                                                                                                                   <  1:490678/250‑1 (MQ=255)                                                                        ttG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTACCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGTTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAgg                                                                                                                                                          <  1:197626/250‑1 (MQ=255)                                                                         gtgcgggtatttggtttGGTATTAGCGGGATCGTGGCAGTGATGATGTCTCGCCCAAAGCCCACGCAGAGGCTAA‑CAATTATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGt                                                                                                                                                         <  2:68054/236‑1 (MQ=255)                                                                                         gggTATTAGCGTGATCCTGGCAGTGATGAGGTAGCGCTCGAAGGCCGCGCAGAAGCTAAACAAT‑TTCGCTTACGCGGTGATTTTTGGCGGCGCGCGGGGCACCC‑TTTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTaaag                                                                                                                                         <  2:547854/250‑4 (MQ=255)                                                                                           gTATTAGCGTGATCCTGGCAGTGATGATGGATCGCTCGAAGGCAACGCAGAACCTAAACAAT‑TTCGCTTCCGGGCTGATTTTTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCTACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGGTTGTGCTGGAAGGTTTTTTACCTTCTAAAGCg                                                                                                                                       <  1:478382/251‑1 (MQ=255)                                                                                               tAGCGTGATCCTGGGAGTGAGGATGTCTCGCTCGAAGGCCCCGCAGAAGCTAAAAAAT‑TTCGCTAACGCTCGTATTATTGGCGGCGCGTTGGGCACCC‑TGTTCGTCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGaaa                                                                                                                                    <  1:226123/250‑1 (MQ=255)                                                                                                aGCGTGATCCTGGATGTGGTGATGTGCCTCGCGAAGGCCACGCAGAAGCGAAACAAT‑TTCCCTTCCGCCCTGAATATTGCCGCCGCGCGGGGAAGCT‑TGTTCGACCGCCTGTGGCACGGCTGCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACGTCGCCACCTTCAAGCTTGCCGATACTGCCATCTGTGTCGGTTCGGCTCTGATTGTGCGGGAAGGTTTTTTACCCTCTAAAGCGaaaa                                                                                                                                   <  2:376144/250‑1 (MQ=255)                                                                                                   gTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACATT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACa                                                                                                                               <  1:467823/251‑1 (MQ=255)                                                                                                      aTCCTGGCGGGGATGTTTTATCGCCCGAGGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGTTTATTGGCGGCGCGCTGGG‑AACCGTGTTCGACCGCCTGTGGCGCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACaata                                                                                                                            <  1:435209/251‑1 (MQ=255)                                                                                                                            gCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatg                                                                                                      <  1:491848/251‑1 (MQ=255)                                                                                                                            gCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACAGCCTGTGGCCCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGgatgcgatg                                                                                                      >  1:291729/1‑251 (MQ=255)                                                                                                                               cgaggcccccgaaGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGTGGGGCACCC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTg                                                                                                   <  2:495115/239‑1 (MQ=255)                                                                                                                               cGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTCCGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTg                                                                                                   >  1:136745/1‑251 (MQ=255)                                                                                                                                    gCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGc                                                                                               >  1:421298/1‑250 (MQ=255)                                                                                                                                     ccACGCAGAAGAGAAACAAT‑TTCGCTTACGCTTTGTTGTTTGGCGGCGCGCTGGGACCCC‑TGTCCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAAGAAACCCTTCCGGATGCGATTCTGACGCAt                                                                                             <  1:433537/251‑1 (MQ=255)                                                                                                                                               gCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGc                                                                                   >  1:549147/1‑251 (MQ=255)                                                                                                                                                                         attattGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTATAAAGCGAAAAAACAATAATAAACCCTGCCGGAGGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAgg                                                          >  1:331980/1‑251 (MQ=255)                                                                                                                                                                           tattGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAACACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGCAGGcc                                                        >  1:214422/1‑251 (MQ=255)                                                                                                                                                                                        cTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATCTGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGCCGCATCTTATCCGGCCTACAGATTGCTGCGACATCGTAGGCCGGATAAGGCGttt                                           >  2:311893/1‑251 (MQ=255)                                                                                                                                                                                              cccc‑TGTTCGCCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                     <  2:349496/249‑1 (MQ=255)                                                                                                                                                                                               ccc‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGAGCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTTCCGATACTGCCATCTGTGTCGTTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTTTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTTACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTAcgccg                                     <  1:54084/249‑1 (MQ=255)                                                                                                                                                                                                  cgtttcaaCCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCAt                                  <  2:176641/244‑1 (MQ=255)                                                                                                                                                                                                      ccgtccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCGTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAAGCGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                               <  2:313576/246‑1 (MQ=255)                                                                                                                                                                                                       cgtccGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                             <  2:113920/248‑1 (MQ=255)                                                                                                                                                                                                                     aCGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAat               <  2:88903/251‑1 (MQ=255)                                                                                                                                                                                                                       ggCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAatat             >  1:224819/1‑251 (MQ=255)                                                                                                                                                                                                                        gCTTCGTTGTCGATATGATAGACTTCTACGTCGGCGGATAGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGAGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTCTCCGGCCTACAGATTGCTGCGAGATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGAAACACTCCTTAAatat             >  1:378968/1‑250 (MQ=255)                                                                                                                                                                                                                             gTTGTCGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGc       <  1:536006/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGATATGATCGACTTCTACGTCGGCTGCTGGCACTTCGCCACCTGCAACCTTGCCGATACTGCCATCTGTGTCGGGGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc  <  1:315455/251‑1 (MQ=255)                                                                                                                                                                                                                                  cGATATGATCGACTTCTACGTCGGCGGCTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTACCTTCTAAAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAAcc  <  2:127950/251‑1 (MQ=255)                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                 CATTATGCGCGTAACTATGGCGCGGCGTTTAGTTTCC‑TTGCCGATAGCGGCGGCTGGCAGCGTTGGTTCTTTG‑CCGGTATTGCGATTGGTATTAGCGTGATCCTGGCAGTGATGATGTATCGCTCGAAGGCCACGCAGAAGCTAAACAAT‑ATCGCTTACGCGCTGATTATTGGCGGCGCGCTGGGCAACC‑TGTTCGACCGCCTGTGGCACGGCTTCGTTGTCGATATGATCGACTTCTACGTCGGCGACTGGCACTTCGCCACCTTCAACCTTGCCGATACTGCCATCTGTGTCGGTGCGGCACTGATTGTGCTGGAAGGTTTTTTGCCTTCTAGAGCGAAAAAACAATAATAAACCCTGCCGGATGCGATGCTGACGCATCTTATCCGGCCTACAGATTGCTGCGAAATCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAAAAATCCTTAAATATAAGAGCAAACC  >  NC_000913/25351‑25822

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑