Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 24,799 G→A A803A (GCG→GCA)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091324,7990GA99.1% 355.5 / NA 110A803A (GCG→GCA) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (45/64);  total (46/64)
Fisher's exact test for biased strand distribution p-value = 4.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGA  >  NC_000913/24553‑25037
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATg                                                                                                                                                                                                                                             <  2:525246/250‑1 (MQ=255)
tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATg                                                                                                                                                                                                                                             >  1:578723/1‑250 (MQ=255)
          tCCTTCTACCTCGACAGCATCAAAGACCGTCAGTACACCGCCAAAGCGGTCAGCGTGTCGCGTCGTAGCTGCCAGAGTGCGCTGTATCACATCGCAGAAGCGCGGGTTCGCTGGATGGCAGCAATCCGCGCCTTCTCCGCTGATGAAGTGGGGGGCTACCGGCCGGGCGTACGTGAAAAATTCGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCATACAGTGAAGCAATGAACGATGCGt                                                                                                                                                                                                                                   <  1:596092/250‑1 (MQ=255)
           ccTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGtt                                                                                                                                                                                                                                  <  1:454608/250‑1 (MQ=255)
           ccTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGtt                                                                                                                                                                                                                                  <  1:186814/250‑1 (MQ=255)
           ccTTCTACCTCGACATCATCAAAGACCGGCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTc                                                                                                                                                                                                                                 <  1:167100/251‑1 (MQ=255)
                 aCCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGa                                                                                                                                                                                                                            <  2:294292/250‑1 (MQ=255)
                  ccTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACg                                                                                                                                                                                                                          >  2:503960/1‑251 (MQ=255)
                    ccGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAg                                                                                                                                                                                                                        <  1:207489/250‑1 (MQ=255)
                     cGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCTTTCTGGGACGa                                                                                                                                                                                                                         <  1:128065/249‑1 (MQ=255)
                      gACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCt                                                                                                                                                                                                                      <  1:458357/251‑1 (MQ=255)
                           catcaAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTg                                                                                                                                                                                                                  >  1:106515/1‑250 (MQ=255)
                                   aCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGt                                                                                                                                                                                                         >  1:354177/1‑251 (MQ=255)
                                     cGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCGGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                                                                                                                                                                                                       <  2:207792/251‑1 (MQ=255)
                                      gTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGGGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGc                                                                                                                                                                                                      <  1:222640/251‑1 (MQ=255)
                                       tCAGAACACCGCCGAAGCGGACAGAGTGGGGCGTCGGAGCTGCCAGATTGCGAGGTATCACATCGCATGAGCGCTGGTTCGCTGGATGGCACAAACCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCGGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTTCGTGGc                                                                                                                                                                                                      <  2:286993/250‑1 (MQ=255)
                                          gTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                                                                                                                                                                                  <  1:322658/251‑1 (MQ=255)
                                           tACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGt                                                                                                                                                                                                 >  1:97427/1‑251 (MQ=255)
                                            aacccGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAGCGTGAAAAATACGTCGTCACCGGTGAGTGGTACGAAGGCCTGTGTGGTCTGGCAGACAGTGAAGAAAAGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGt                                                                                                                                                                                                 <  2:506124/247‑1 (MQ=255)
                                                 gCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACa                                                                                                                                                                                            >  1:138333/1‑250 (MQ=255)
                                                  ccAAAGCGGACAGGGTGTCGCGTCGGAGCTGCCAGACTGCGCTGTATCACAGCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCGCCTGCACCGCTGATGAAGTGTGGGGTTACCTGCCGGGCGAACGTGAAAAATACGTCTGCACCGGTGAGTGGGACGAAGGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGTGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACaaa                                                                                                                                                                                          <  2:361123/251‑1 (MQ=255)
                                                   cAAAGCGGACAGCGGGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCCGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAg                                                                                                                                                                                         <  2:15159/251‑1 (MQ=255)
                                                    aaaGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGt                                                                                                                                                                                        >  1:57090/1‑251 (MQ=255)
                                                        cGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCAtt                                                                                                                                                                                    >  1:44166/1‑251 (MQ=255)
                                                         ggACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                                                                                                                                                                                   <  1:284242/251‑1 (MQ=255)
                                                           aCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAg                                                                                                                                                                                 <  1:209046/251‑1 (MQ=255)
                                                            cagcgtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGc                                                                                                                                                                                <  2:518541/247‑1 (MQ=255)
                                                             agcgtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGc                                                                                                                                                                                >  2:148813/4‑250 (MQ=255)
                                                                gtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                                                                                                                                                            >  2:111895/1‑251 (MQ=255)
                                                                 tgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcg                                                                                                                                                                           >  1:613494/1‑251 (MQ=255)
                                                                  ggCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcgc                                                                                                                                                                          <  2:72477/251‑1 (MQ=255)
                                                                    cgcgTCGTAGATGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGCCAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGCAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGt                                                                                                                                                                        >  1:110423/1‑251 (MQ=255)
                                                                     gcgTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCGCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTg                                                                                                                                                                       <  2:570533/251‑1 (MQ=255)
                                                                      cgtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGc                                                                                                                                                                      >  1:80528/1‑251 (MQ=255)
                                                                         cgtAGCTGCCAGACGGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAAGCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGa                                                                                                                                                                   <  2:137828/251‑1 (MQ=255)
                                                                           tAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGAc                                                                                                                                                                  >  2:244051/1‑250 (MQ=255)
                                                                           tAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGACTGGCACCAATCCTCTCCTTCACCGCTGATGACGTGTGGGGCTACCTGCCGGGCGCACGTGACAAATACGTCTTCACCGGTGAGTGGAACGAAGGCCTGTTTGGTCTCGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGCTGAACGTCCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGCGCCGAc                                                                                                                                                                  >  1:42004/1‑250 (MQ=255)
                                                                            aGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                                                                                                                                                                                     >  2:323829/1‑214 (MQ=255)
                                                                            aGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                                                                                                                                                                                     <  1:323829/214‑1 (MQ=255)
                                                                                 ccAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGaa                                                                                                                                                             >  1:472661/1‑249 (MQ=255)
                                                                                   aGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtg                                                                                                                                                         >  1:43976/1‑251 (MQ=255)
                                                                                        gcgcTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtgggtgg                                                                                                                                                    >  2:410302/1‑251 (MQ=255)
                                                                                        gcgcTGTATCACATCGCAGAAGCGCTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGGGGGGCGACGTGCCGGGGGAACGTGAAAAATGCGTCTTCACCGGGGAGGGGTACGAATGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAAGGATGCGGTCTGGGACGAGCGGTTGAAAGTGCGGGGCGATGTGATCAAAGTCATTGAGCTGGCGGGTTCCGACAAGGAAgtgggt                                                                                                                                                      <  2:362008/249‑1 (MQ=255)
                                                                                         agctgtatCACATCGCAGAATAGCTGATTCGCTGGATGGCACCACTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGACGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGACTGTTTGGTCAGGCAGACAGTGAAGCGATGACCGATGAGTTCTGGGACGAGCTATTGAAAGTGCGTGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGCGCCGACAAGCAAGTGGGTGGc                                                                                                                                                   >  2:404402/6‑251 (MQ=255)
                                                                                               atCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCt                                                                                                                                              <  1:227475/250‑1 (MQ=255)
                                                                                               atCACATCGAAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGGGGGGCTACCTGCCGGGCGAACGTGAAAAAGACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCGGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTg                                                                                                                                             <  2:53851/251‑1 (MQ=255)
                                                                                                      cGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCgg                                                                                                                                      >  1:250969/1‑251 (MQ=255)
                                                                                                       gCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGc                                                                                                                                     <  2:68876/251‑1 (MQ=255)
                                                                                                             gcgcTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGGGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAAc                                                                                                                               <  1:388108/251‑1 (MQ=255)
                                                                                                                cgggttcgcTGGATGGCACCAATCCGCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGGCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTTGCTCTCTGGAACCGGCAGTAACCtt                                                                                                                            <  2:33643/245‑1 (MQ=255)
                                                                                                                 tGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTg                                                                                                                           >  1:18039/1‑251 (MQ=255)
                                                                                                                  ggttcgcTGGATGGCACCAATCCTCTCCTTCAACGCTGATGAAGTGTGGGGCTACCTGCCGTGCGAGCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCtt                                                                                                                            <  1:165597/245‑1 (MQ=255)
                                                                                                                  ggTGCGCTGGAGGGCGCCAATCCGCTCCTTCCCCGCTGATGAAGTGTTGGGCTTCCTGCCGGGCGAACGTGAAAAATACGTCTTCCCCTGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACATTGAAGCAATGAACGAGGCGTTCTGGGACGGGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAATCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCtt                                                                                                                            <  1:315548/249‑1 (MQ=255)
                                                                                                                           gATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCGGAAc                                                                                                                 <  2:483493/251‑1 (MQ=255)
                                                                                                                              ggCACCAATCCTCTCCTTCACCGCTTATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACTTTGTATGCAGAACCgg                                                                                                              <  1:426243/251‑1 (MQ=255)
                                                                                                                                cACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGaa                                                                                                            <  2:506299/251‑1 (MQ=255)
                                                                                                                                     aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTggg                                                                                                       >  2:186704/1‑249 (MQ=255)
                                                                                                                                        ctctCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGACGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAATGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGCAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGCACTggcggc                                                                                                    >  1:385378/1‑251 (MQ=255)
                                                                                                                                             cTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAc                                                                                               >  1:431669/1‑251 (MQ=255)
                                                                                                                                               tccccGCTGATGAAGTGTGGGGCTACCGGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGGTTGGGCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAAGCTTGTATGCAGAACCGGAACTGGCGGCGAAACt                                                                                              <  2:45945/247‑1 (MQ=255)
                                                                                                                                               tCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAc                                                                                               <  1:274859/249‑1 (MQ=255)
                                                                                                                                               tCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAc                                                                                               <  2:422407/249‑1 (MQ=255)
                                                                                                                                                   cGCTGATGAAGTGTGGGGCTACCTGGCGGGCGAACGTGAAAAATACGGCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACcg                                                                                         <  1:573343/251‑1 (MQ=255)
                                                                                                                                                    gCTGAAGAAGTGTGGGACTACCTTCCGGGCGAACGTGTAAAATACGTCTTCACCGGGGTGTGGTACGACAGCCCGTTTGGTATGGCAGTCAGTGTAGCAATGAACGATGCTTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGTGCCGACACGAAAGTGGGTGGCTCGCTGGCCGCGGCAGTACCCTTGTATGCAGAACCGGACCTGgcggcgcacatgaccgc                                                                                        >  2:591537/1‑240 (MQ=255)
                                                                                                                                                       gatgaAGTGTGTGGCTACCTGCCGGGCTAACGTGAAAAATACGTCGTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAATCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCt                                                                                     <  1:347511/251‑1 (MQ=255)
                                                                                                                                                       gatgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACcgcg                                                                                       <  1:178680/249‑1 (MQ=255)
                                                                                                                                                          gaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCCGAACCGGAACTGGCGGCGAAACTGACCGCGCTggg                                                                                  >  2:510068/1‑251 (MQ=255)
                                                                                                                                                            aGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCg                                                                                <  1:214013/251‑1 (MQ=255)
                                                                                                                                                                           gCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTg                                                                 <  1:521329/251‑1 (MQ=255)
                                                                                                                                                                           gCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGAGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGAtt                                                                   <  1:510403/249‑1 (MQ=255)
                                                                                                                                                                             cGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGCAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTggc                                                               >  2:226065/1‑249 (MQ=255)
                                                                                                                                                                               ggCGAACGTGAAAAATACGATTTCACCGGTGAGTGGGACGAAGGCCTGTTTGGTCTGGCAGACCGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTGGAATGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGCGGCTCGCTGGAAGCGGCAGTAACCTTGAATGCAGAACCGGGACTGGCGGCGAAACTTAGCCCCCTGGGCGATGACTTACGATTTGTctt                                                             >  2:566254/1‑249 (MQ=255)
                                                                                                                                                                                  gAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTggcttgtt                                                          <  2:80528/251‑8 (MQ=255)
                                                                                                                                                                                   aaCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCttgttg                                                         <  1:503960/251‑1 (MQ=255)
                                                                                                                                                                                     cGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAGGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGGAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTTTTGAc                                                       >  2:440526/1‑251 (MQ=255)
                                                                                                                                                                                      gTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGAcc                                                      <  2:181834/251‑1 (MQ=255)
                                                                                                                                                                                           aaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCgg                                                 <  2:503638/251‑1 (MQ=255)
                                                                                                                                                                                            aaTACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgc                                                <  2:475622/251‑1 (MQ=255)
                                                                                                                                                                                               aCGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCt                                             <  1:343945/251‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                                           <  2:586617/251‑1 (MQ=255)
                                                                                                                                                                                                 gTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                                           >  1:273283/1‑251 (MQ=255)
                                                                                                                                                                                                 gTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                                           <  2:179816/251‑1 (MQ=255)
                                                                                                                                                                                                     tCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                                       >  1:543310/1‑251 (MQ=255)
                                                                                                                                                                                                     tCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGt                                        <  1:410302/250‑1 (MQ=255)
                                                                                                                                                                                                     tCACCGGTGAGGGGTACGAAGGCCTGTGTGGTCTGGCAGACAGTGAAGAAAAGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCg                                         <  2:43606/249‑1 (MQ=255)
                                                                                                                                                                                                      cACCGGGGAGTGGTACGAAGGCCTGTTTGGGATGGCAGACAGGGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGTCCGCGCTGGGCTATGAATTGCGATTTGTCTTGTTGAcccccttcgctatctttg                                      <  2:75137/251‑17 (MQ=255)
                                                                                                                                                                                                        ccGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCa                                    <  2:18596/251‑1 (MQ=255)
                                                                                                                                                                                                              aGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACtat                              >  1:221047/1‑251 (MQ=255)
                                                                                                                                                                                                              aGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACtat                              >  1:287707/1‑251 (MQ=255)
                                                                                                                                                                                                                 ggTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAac                           <  1:252206/251‑1 (MQ=255)
                                                                                                                                                                                                                      gAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGc                      >  1:84442/1‑251 (MQ=255)
                                                                                                                                                                                                                           ccTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctg                 >  1:297304/1‑251 (MQ=255)
                                                                                                                                                                                                                             tGTTTGGTCTGGCAGACAGTGAAGCAATGAAAGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct               >  1:516164/1‑251 (MQ=255)
                                                                                                                                                                                                                              gTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgctg              >  1:17342/1‑251 (MQ=255)
                                                                                                                                                                                                                                     cTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGGGGCTCGCTGGTAGCGGCAGTAACCTTGTATGCAGAACAGGAACTGGCGGCGAAACTGACCGCGCTGGGAGCTGAATTACAATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGATGATAACGCCGCACATGCTGATGccc        >  2:478382/1‑248 (MQ=255)
                                                                                                                                                                                                                                       ggCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcag      <  2:280811/250‑1 (MQ=255)
                                                                                                                                                                                                                                       ggCAGACAGTGAAGCAATGAACGAGGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTc        <  2:68427/248‑1 (MQ=255)
                                                                                                                                                                                                                                        gCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAACGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGAACATGCTGATGCTccgc     >  2:124635/1‑247 (MQ=255)
                                                                                                                                                                                                                                        gCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcagca    <  1:235628/251‑1 (MQ=255)
                                                                                                                                                                                                                                          aGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGAGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTcagcagg  >  1:233070/1‑250 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
TGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTGATGCTCAGCAGA  >  NC_000913/24553‑25037

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑