BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,781	C→T	G797G (GGC→GGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,781	0	C	T	96.3%	341.5 / NA	110	G797G (GGC→GGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (45/60); minor base G (0/4); total (46/64)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 110 total aligned reads displayed.

TGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTG  >  NC_000913/24534‑25025

tGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTg                                                                                                                                                                                                                                                   >  1:503638/1‑251 (MQ=255)

 gATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCACTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTgg                                                                                                                                                                                                                                                  >  1:376617/1‑251 (MQ=255)

         tCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACa                                                                                                                                                                                                                                            <  1:31862/249‑1 (MQ=255)

         tCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAg                                                                                                                                                                                                                                           <  1:121686/250‑1 (MQ=255)

           ggcgccGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGa                                                                                                                                                                                                                                        <  2:66592/247‑1 (MQ=255)

                   tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATg                                                                                                                                                                                                                                 <  2:525246/250‑1 (MQ=255)

                   tGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATg                                                                                                                                                                                                                                 >  1:578723/1‑250 (MQ=255)

                         gggTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATg                                                                                                                                                                                                                          >  1:586617/1‑251 (MQ=255)

                             tCCTTCTACCTCGACAGCATCAAAGACCGTCAGTACACCGCCAAAGCGGTCAGCGTGTCGCGTCGTAGCTGCCAGAGTGCGCTGTATCACATCGCAGAAGCGCGGGTTCGCTGGATGGCAGCAATCCGCGCCTTCTCCGCTGATGAAGTGGGGGGCTACCGGCCGGGCGTACGTGAAAAATTCGGCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCATACAGTGAAGCAATGAACGATGCGt                                                                                                                                                                                                                       <  1:596092/250‑1 (MQ=255)

                              ccTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGtt                                                                                                                                                                                                                      <  1:186814/250‑1 (MQ=255)

                              ccTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGtt                                                                                                                                                                                                                      <  1:454608/250‑1 (MQ=255)

                              ccTTCTACCTCGACATCATCAAAGACCGGCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTc                                                                                                                                                                                                                     <  1:167100/251‑1 (MQ=255)

                                     ccTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACg                                                                                                                                                                                                              >  2:503960/1‑251 (MQ=255)

                                       ccGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAg                                                                                                                                                                                                            <  1:207489/250‑1 (MQ=255)

                                        cGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCTTTCTGGGACGa                                                                                                                                                                                                             <  1:128065/249‑1 (MQ=255)

                                         gACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCt                                                                                                                                                                                                          <  1:458357/251‑1 (MQ=255)

                                              catcaAAGACCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTg                                                                                                                                                                                                      >  1:106515/1‑250 (MQ=255)

                                                   aaGACCGTCAGTACACCGCCAAAGCGGACAGCGAGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTg                                                                                                                                                                                                >  1:203207/1‑251 (MQ=255)

                                                      aCCGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGt                                                                                                                                                                                             >  1:354177/1‑251 (MQ=255)

                                                        cGTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCGGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTgg                                                                                                                                                                                           <  2:207792/251‑1 (MQ=255)

                                                         gTCAGTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGGGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGc                                                                                                                                                                                          <  1:222640/251‑1 (MQ=255)

                                                          tCAGAACACCGCCGAAGCGGACAGAGTGGGGCGTCGGAGCTGCCAGATTGCGAGGTATCACATCGCATGAGCGCTGGTTCGCTGGATGGCACAAACCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCGGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTTCGTGGc                                                                                                                                                                                          <  2:286993/250‑1 (MQ=255)

                                                             gTACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAg                                                                                                                                                                                      <  1:322658/251‑1 (MQ=255)

                                                              tACACCGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGt                                                                                                                                                                                     >  1:97427/1‑251 (MQ=255)

                                                               aacccGCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCGTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAGCGTGAAAAATACGTCGTCACCGGTGAGTGGTACGAAGGCCTGTGTGGTCTGGCAGACAGTGAAGAAAAGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGt                                                                                                                                                                                     <  2:506124/247‑1 (MQ=255)

                                                                    gCCAAAGCGGACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACa                                                                                                                                                                                >  1:138333/1‑250 (MQ=255)

                                                                     ccAAAGCGGACAGGGTGTCGCGTCGGAGCTGCCAGACTGCGCTGTATCACAGCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCGCCTGCACCGCTGATGAAGTGTGGGGTTACCTGCCGGGCGAACGTGAAAAATACGTCTGCACCGGTGAGTGGGACGAAGGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGTGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACaaa                                                                                                                                                                              <  2:361123/251‑1 (MQ=255)

                                                                      cAAAGCGGACAGCGGGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCCCATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCCGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAg                                                                                                                                                                             <  2:15159/251‑1 (MQ=255)

                                                                            ggACAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTg                                                                                                                                                                       <  1:284242/251‑1 (MQ=255)

                                                                              aCAGCGTGGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAg                                                                                                                                                                     <  1:209046/251‑1 (MQ=255)

                                                                               cagcgtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGc                                                                                                                                                                    <  2:518541/247‑1 (MQ=255)

                                                                                 gcgtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCaa                                                                                                                                                                  <  1:458027/249‑1 (MQ=255)

                                                                                   gtgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgc                                                                                                                                                                >  2:111895/1‑251 (MQ=255)

                                                                                    tgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcg                                                                                                                                                               >  1:613494/1‑251 (MQ=255)

                                                                                    tgGCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcg                                                                                                                                                               >  2:316114/1‑251 (MQ=255)

                                                                                     ggCGCGTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAgcgc                                                                                                                                                              <  2:72477/251‑1 (MQ=255)

                                                                                       cgcgTCGTAGATGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGCCAGTGAAGCAATGAACGATGCGTTCTGGGACGCGCTGTTGCAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGt                                                                                                                                                            >  1:110423/1‑251 (MQ=255)

                                                                                        gcgTCGTAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCGCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTg                                                                                                                                                           <  2:570533/251‑1 (MQ=255)

                                                                                         cgtcgtAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGc                                                                                                                                                          >  1:80528/1‑251 (MQ=255)

                                                                                            cgtAGCTGCCAGACGGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAAGCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGa                                                                                                                                                       <  2:137828/251‑1 (MQ=255)

                                                                                              tAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGAc                                                                                                                                                      >  2:244051/1‑250 (MQ=255)

                                                                                              tAGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGACTGGCACCAATCCTCTCCTTCACCGCTGATGACGTGTGGGGCTACCTGCCGGGCGCACGTGACAAATACGTCTTCACCGGTGAGTGGAACGAAGGCCTGTTTGGTCTCGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGCTGAACGTCCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGCGCCGAc                                                                                                                                                      >  1:42004/1‑250 (MQ=255)

                                                                                               aGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                                                                                                                                                                         <  1:323829/214‑1 (MQ=255)

                                                                                               aGCTGCCAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCg                                                                                                                                                                                         >  2:323829/1‑214 (MQ=255)

                                                                                                    ccAGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGaa                                                                                                                                                 >  1:472661/1‑249 (MQ=255)

                                                                                                      aGACTGCGCTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtg                                                                                                                                             >  1:43976/1‑251 (MQ=255)

                                                                                                           gcgcTGTATCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAgtgggtgg                                                                                                                                        >  2:410302/1‑251 (MQ=255)

                                                                                                           gcgcTGTATCACATCGCAGAAGCGCTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGGGGGGCGACGTGCCGGGGGAACGTGAAAAATGCGTCTTCACCGGGGAGGGGTACGAATGCCTGTTTGGGCTGGCAGACAGTGAAGCAATGAAGGATGCGGTCTGGGACGAGCGGTTGAAAGTGCGGGGCGATGTGATCAAAGTCATTGAGCTGGCGGGTTCCGACAAGGAAgtgggt                                                                                                                                          <  2:362008/249‑1 (MQ=255)

                                                                                                            agctgtatCACATCGCAGAATAGCTGATTCGCTGGATGGCACCACTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGACGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGACTGTTTGGTCAGGCAGACAGTGAAGCGATGACCGATGAGTTCTGGGACGAGCTATTGAAAGTGCGTGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGCGCCGACAAGCAAGTGGGTGGc                                                                                                                                       >  2:404402/6‑251 (MQ=255)

                                                                                                                  atCACATCGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCt                                                                                                                                  <  1:227475/250‑1 (MQ=255)

                                                                                                                  atCACATCGAAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGGGGGGCTACCTGCCGGGCGAACGTGAAAAAGACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCGGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTg                                                                                                                                 <  2:53851/251‑1 (MQ=255)

                                                                                                                         cGCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCgg                                                                                                                          >  1:250969/1‑251 (MQ=255)

                                                                                                                          gCAGAAGCGCTGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGc                                                                                                                         <  2:68876/251‑1 (MQ=255)

                                                                                                                                gcgcTGGGTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGGGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAAc                                                                                                                   <  1:388108/251‑1 (MQ=255)

                                                                                                                                   cgggttcgcTGGATGGCACCAATCCGCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGGCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTTGCTCTCTGGAACCGGCAGTAACCtt                                                                                                                <  2:33643/245‑1 (MQ=255)

                                                                                                                                    tGGTTCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTg                                                                                                               >  1:18039/1‑251 (MQ=255)

                                                                                                                                     ggttcgcTGGATGGCACCAATCCTCTCCTTCAACGCTGATGAAGTGTGGGGCTACCTGCCGTGCGAGCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCtt                                                                                                                <  1:165597/245‑1 (MQ=255)

                                                                                                                                     ggTGCGCTGGAGGGCGCCAATCCGCTCCTTCCCCGCTGATGAAGTGTTGGGCTTCCTGCCGGGCGAACGTGAAAAATACGTCTTCCCCTGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACATTGAAGCAATGAACGAGGCGTTCTGGGACGGGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAATCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCtt                                                                                                                <  1:315548/249‑1 (MQ=255)

                                                                                                                                              gATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCGGAAc                                                                                                     <  2:483493/251‑1 (MQ=255)

                                                                                                                                                 ggCACCAATCCTCTCCTTCACCGCTTATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACTTTGTATGCAGAACCgg                                                                                                  <  1:426243/251‑1 (MQ=255)

                                                                                                                                                   cACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGaa                                                                                                <  2:506299/251‑1 (MQ=255)

                                                                                                                                                        aTCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTggg                                                                                           >  2:186704/1‑249 (MQ=255)

                                                                                                                                                           ctctCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGACGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAATGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGCAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGCACTggcggc                                                                                        >  1:385378/1‑251 (MQ=255)

                                                                                                                                                                cTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAc                                                                                   >  1:431669/1‑251 (MQ=255)

                                                                                                                                                                  tCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAc                                                                                   <  1:274859/249‑1 (MQ=255)

                                                                                                                                                                  tCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAAc                                                                                   <  2:422407/249‑1 (MQ=255)

                                                                                                                                                                      cGCTGATGAAGTGTGGGGCTACCTGGCGGGCGAACGTGAAAAATACGGCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACcg                                                                             <  1:573343/251‑1 (MQ=255)

                                                                                                                                                                       gCTGAAGAAGTGTGGGACTACCTTCCGGGCGAACGTGTAAAATACGTCTTCACCGGGGTGTGGTACGACAGCCCGTTTGGTATGGCAGTCAGTGTAGCAATGAACGATGCTTTCTGGGACGAGCTGTTGAACGTGCGTGGCGAAGGGAACAAAGTCATTGAGCAAGCGCGTGCCGACACGAAAGTGGGTGGCTCGCTGGCCGCGGCAGTACCCTTGTATGCAGAACCGGACCTGgcggcgcacatgaccgc                                                                            >  2:591537/1‑240 (MQ=255)

                                                                                                                                                                          gatgaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACcgcg                                                                           <  1:178680/249‑1 (MQ=255)

                                                                                                                                                                             gaAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCCGAACCGGAACTGGCGGCGAAACTGACCGCGCTggg                                                                      >  2:510068/1‑251 (MQ=255)

                                                                                                                                                                               aGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCg                                                                    <  1:214013/251‑1 (MQ=255)

                                                                                                                                                                                              gCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTg                                                     <  1:521329/251‑1 (MQ=255)

                                                                                                                                                                                              gCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGAGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGAtt                                                       <  1:510403/249‑1 (MQ=255)

                                                                                                                                                                                                cGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGCAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAACGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTggc                                                   >  2:226065/1‑249 (MQ=255)

                                                                                                                                                                                                  ggCGAACGTGAAAAATACGATTTCACCGGTGAGTGGGACGAAGGCCTGTTTGGTCTGGCAGACCGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTGGAATGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGCGGCTCGCTGGAAGCGGCAGTAACCTTGAATGCAGAACCGGGACTGGCGGCGAAACTTAGCCCCCTGGGCGATGACTTACGATTTGTctt                                                 >  2:566254/1‑249 (MQ=255)

                                                                                                                                                                                                   gCGAACGGGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGTCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTcttg                                                <  2:178611/251‑4 (MQ=255)

                                                                                                                                                                                                     gAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTggcttgtt                                              <  2:80528/251‑8 (MQ=255)

                                                                                                                                                                                                      aaCGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCttgttg                                             <  1:503960/251‑1 (MQ=255)

                                                                                                                                                                                                        cGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAGGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGGAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTTTTGAc                                           >  2:440526/1‑251 (MQ=255)

                                                                                                                                                                                                         gTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGAcc                                          <  2:181834/251‑1 (MQ=255)

                                                                                                                                                                                                              aaaTACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCgg                                     <  2:503638/251‑1 (MQ=255)

                                                                                                                                                                                                               aaTACGTCTTCACCGGGGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGgc                                    <  2:475622/251‑1 (MQ=255)

                                                                                                                                                                                                                  aCGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCt                                 <  1:343945/251‑1 (MQ=255)

                                                                                                                                                                                                                    gTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                               <  2:179816/251‑1 (MQ=255)

                                                                                                                                                                                                                    gTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                               >  1:273283/1‑251 (MQ=255)

                                                                                                                                                                                                                    gTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTAc                               <  2:586617/251‑1 (MQ=255)

                                                                                                                                                                                                                        tCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGtt                           >  1:543310/1‑251 (MQ=255)

                                                                                                                                                                                                                        tCACCGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGt                            <  1:410302/250‑1 (MQ=255)

                                                                                                                                                                                                                        tCACCGGTGAGGGGTACGAAGGCCTGTGTGGTCTGGCAGACAGTGAAGAAAAGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCg                             <  2:43606/249‑1 (MQ=255)

                                                                                                                                                                                                                         cACCGGGGAGTGGTACGAAGGCCTGTTTGGGATGGCAGACAGGGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTACCCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGTCCGCGCTGGGCTATGAATTGCGATTTGTCTTGTTGAcccccttcgctatctttg                          <  2:75137/251‑17 (MQ=255)

                                                                                                                                                                                                                           ccGGTGAGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCa                        <  2:18596/251‑1 (MQ=255)

                                                                                                                                                                                                                                 aGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACtat                  >  1:221047/1‑251 (MQ=255)

                                                                                                                                                                                                                                 aGTGGTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACtat                  >  1:287707/1‑251 (MQ=255)

                                                                                                                                                                                                                                  gTGGTACGAAGGCCTGGTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACtata                 <  2:472661/251‑1 (MQ=255)

                                                                                                                                                                                                                                    ggTACGAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAac               <  1:252206/251‑1 (MQ=255)

                                                                                                                                                                                                                                         gAAGGCCTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGc          >  1:84442/1‑251 (MQ=255)

                                                                                                                                                                                                                                              ccTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctg     >  1:297304/1‑251 (MQ=255)

                                                                                                                                                                                                                                               cTGTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACTTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgc    >  1:352286/1‑251 (MQ=255)

                                                                                                                                                                                                                                                tGTTTGGTCTGGCAGACAGTGAAGCAATGAAAGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgct   >  1:516164/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 gTTTGGTCTGGCAGACAGTGAAGCAATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGGCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCTTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACctgctg  >  1:17342/1‑251 (MQ=255)

TGATGCGCTTCTGCTCCGTTGAGATGGGTTCCTTCTACCTCGACATCATCAAAGACCGTCAGTACACCGCCAAAGCGGACAGTGTGGCGCGTCGTAGCTGCCAGACTGCGCTATATCACATCGCAGAAGCGCTGGTGCGCTGGATGGCACCAATCCTCTCCTTCACCGCTGATGAAGTGTGGGGCTACCTGCCGGGCGAACGTGAAAAATACGTCTTCACCGGTGAGTGGTACGAAGGCCTGTTTGGCCTGGCAGACAGTGAAGCGATGAACGATGCGTTCTGGGACGAGCTGTTGAAAGTGCGTGGCGAAGTGAACAAAGTCATTGAGCAAGCGCGTGCCGACAAGAAAGTGGGTGGCTCGCTGGAAGCGGCAGTAACCTTGTATGCAGAACCGGAACTGTCGGCGAAACTGACCGCGCTGGGCGATGAATTACGATTTGTCCTGTTGACCTCCGGCGCTACCGTTGCAGACTATAACGACGCACCTGCTG  >  NC_000913/24534‑25025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`