Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	24,094	C→T	G568G (GGC→GGT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	24,094	0	C	T	99.0%	306.8 / NA	97	G568G (GGC→GGT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (44/52);  minor base G (0/1);  total (44/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

CCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGC  >  NC_000913/23849‑24336                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGGGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATAC‑TCGCGACGTATGGTTTGACGCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACTCTGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                                                                                                                                                                                                                                <  2:37290/250‑1 (MQ=255)      tACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                                                                                                                                                          >  2:346732/1‑251 (MQ=255)            cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTGAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                                                                                                                                                                                                                                    <  1:608457/250‑1 (MQ=255)                  gATGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCTGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAAt                                                                                                                                                                                                                                <  2:531295/249‑1 (MQ=255)                   aTGGAAGAAGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAt                                                                                                                                                                                                                             >  1:135093/1‑251 (MQ=255)                      gaagaaGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtctc                                                                                                                                                                                                                          >  1:488677/1‑251 (MQ=255)                      gaagaaGTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtctc                                                                                                                                                                                                                          <  1:536829/251‑1 (MQ=255)                        agaagTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTTCCGGATACGCTC‑GACGTATGTTTTGACTCCGGATCTACCGACTCGTCTGTTGTTGACGTGCGTCCGTAATGTGCCGGTTGTGCAGCGGACATGTATCTGTAATGTTCTGACCAGCGCCGTGGGTGGGGCATGTCTTCCCGAATGATCTCCa                                                                                                                                                                                                                        <  2:64937/251‑1 (MQ=255)                        agaagTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTACATGTCTTCCCTAATGATCTCCa                                                                                                                                                                                                                        <  1:168967/251‑1 (MQ=255)                        agaagTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGGGGTGGGATCGCGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCa                                                                                                                                                                                                                        <  2:548485/251‑1 (MQ=255)                          aagTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTCATGATCTCCAcc                                                                                                                                                                                                                      >  2:379053/1‑251 (MQ=255)                            gTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgc                                                                                                                                                                                                                    >  1:263487/1‑251 (MQ=255)                            gTGGCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCCCcgc                                                                                                                                                                                                                    >  2:492670/1‑251 (MQ=255)                             gggCAAAACGCGTGGAAGTTAACGGCATCCCGGCGTGGTGGGATCTCGAGGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgct                                                                                                                                                                                                                   <  2:335793/250‑2 (MQ=255)                              ggCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTc                                                                                                                                                                                                                                              <  1:532055/223‑1 (MQ=255)                              ggCAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcg                                                                                                                                                                                                                   <  1:602125/250‑1 (MQ=255)                              ggCAAAACGCGTTGAAGTTAAAGGCATCCAGGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAAGCTGACCC‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCACCGTGGTTGGTTc                                                                                                                                                                                                                                              >  2:532055/1‑223 (MQ=255)                                cAAAACGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcgctg                                                                                                                                                                                                                >  1:304193/1‑248 (MQ=255)                                 aaaaCGCGTAGAAGTTAACGGCATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGA‑CACGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcgctg                                                                                                                                                                                                                >  2:430957/1‑247 (MQ=255)                                  aaaCGCGTTGAAGTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa                                                                                                                                                                                                              <  1:29746/251‑1 (MQ=255)                                                taacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCg                                                                                                                                                                                                 <  1:108158/246‑1 (MQ=255)                                                 aacggCATCCAGGCGTGGTGGGAGCTCGATGCGAAAGAGAGCCTCGGCGACGAAGCTTACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTCGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTa                                                                                                                                                                                               <  2:144663/248‑1 (MQ=255)                                                  acggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTAt                                                                                                                                                                                              >  1:21136/3‑251 (MQ=255)                                                      cATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTc                                                                                                                                                                                          <  1:118420/251‑1 (MQ=255)                                                       aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCa                                                                                                                                                                                         <  1:21294/251‑1 (MQ=255)                                                       aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGCGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTGGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCTCCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGTGCCGTATCGTCa                                                                                                                                                                                         <  2:585550/251‑1 (MQ=255)                                                       aTCCAGGCGTGGTCGGATCTCGATGCGACAGTGCTCCTCGGCGAAGAAGCTGCCCA‑GCATGTGCGAGGCCCGGATCCTCTC‑GACGTATGGTTTGACTCCGGATCTACACACTCTTCTGTTGTTGACGTGCGTCAGGAATTTACCGGTCACGCAGCGGACATGTATCTGGCAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAACGGCACAGCGCCGTATCGTcc                                                                                                                                                                                         >  2:365584/1‑250 (MQ=255)                                                           aGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGt                                                                                                                                                                                      >  1:588770/1‑250 (MQ=255)                                                            ggCGTGGTGGTATCTCGAGGCGAAAGAGAGCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGCCCGGAAGTTGGCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGCCCAGAACCGTGGTTGGGTCATGTCTTTCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTAc                                                                                                                                                                                    <  1:362584/251‑1 (MQ=255)                                                              cGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTg                                                                                                                                                                                  >  1:458880/1‑251 (MQ=255)                                                                  gtggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGAccc                                                                                                                                                                              <  2:488677/251‑1 (MQ=255)                                                                  gtggGATCTCGATGCGAAAGAGATACTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGCTCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGACAGGCAAAGCGACGTCTCGTCAGGTACTGAccc                                                                                                                                                                              >  2:582385/1‑251 (MQ=255)                                                                     ggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACg                                                                                                                                                                           <  1:209993/251‑1 (MQ=255)                                                                       aTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGc                                                                                                                                                                         >  2:61464/1‑251 (MQ=255)                                                                        tctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCTCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCt                                                                                                                                                                        >  1:128873/1‑251 (MQ=255)                                                                           cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCttt                                                                                                                                                                      <  1:486453/250‑1 (MQ=255)                                                                           cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACgg                                                                                                                                                                          <  1:418771/246‑1 (MQ=255)                                                                           cGATGCGAAAGAGAGCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAGCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCGCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACgg                                                                                                                                                                          <  2:275894/246‑1 (MQ=255)                                                                                cGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTg                                                                                                                                                                <  1:10049/251‑1 (MQ=255)                                                                                  aaaGAGATCCACGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTgg                                                                                                                                                               >  1:478061/1‑250 (MQ=255)                                                                                   aaGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGAt                                                                                                                                                             <  1:1368/251‑1 (MQ=255)                                                                                       gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                                                                                                                                                         >  2:143899/1‑251 (MQ=255)                                                                                       gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                                                                                                                                                         <  1:584206/251‑1 (MQ=255)                                                                                       gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAAGGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGTCCCACGGCTTTACCGTGGATGGTc                                                                                                                                                         <  1:57253/251‑1 (MQ=255)                                                                                       gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGGATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                                                                                                                                                         >  1:515005/1‑251 (MQ=255)                                                                                          ccTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAgg                                                                                                                                                      >  1:11777/1‑251 (MQ=255)                                                                                             cGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                                                                                                                                                   >  1:330591/1‑251 (MQ=255)                                                                                             cGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                                                                                                                                                   <  1:456232/251‑1 (MQ=255)                                                                                              ggCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GAAGTATGGTTTGACTCCGGATCTACCCACTCTTATGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                                                                                                                                                  >  1:32042/1‑251 (MQ=255)                                                                                              ggCGACGAAGCTGACCA‑GGATGTGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                                                                                                                                                  <  2:60828/251‑1 (MQ=255)                                                                                                cgacgaAGCTGACCA‑GTATGTGAAAGTGCCGGATTCTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                                <  2:129163/251‑1 (MQ=255)                                                                                                 gacgaAGCTGACCA‑GTATGTGACAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                                                                                                                                                >  2:279765/1‑250 (MQ=255)                                                                                                   ggaAGCTGACCA‑GTATGTGAAATTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGGTGACGTGCGTCCGGAATTTGCCGGTCACGCAGGGGACAGGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTGATGATCTCCACCGCGATGGAAGGCAAAGCGCCGTATCGTCAGGTACGGACCCACGGCTTTACCTTGGATGGTCATGGCCGCAAGa                                                                                                                                             <  2:281514/250‑1 (MQ=255)                                                                                                   cgaAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGa                                                                                                                                             <  1:87783/251‑1 (MQ=255)                                                                                                    gaAGCTGACCA‑GTATGTGAAAGTGCCGGATAC‑TCGCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGAt                                                                                                                                            <  2:2441/251‑1 (MQ=255)                                                                                                          gaccA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCt                                                                                                                                        <  2:73093/246‑1 (MQ=255)                                                                                                                gtatgTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCa                                                                                                                                 <  1:442099/247‑1 (MQ=255)                                                                                                                gtatgTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCa                                                                                                                                 <  2:515005/247‑1 (MQ=255)                                                                                                                       gaaagtgacggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGcc                                                                                                                           >  2:415369/23‑250 (MQ=255)                                                                                                                         aagtgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTTATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                         <  1:50343/231‑2 (MQ=255)                                                                                                                          agtgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaacc                                                                                                                        >  2:17570/20‑249 (MQ=255)                                                                                                                          agtgccggatactctcgacgTATGGATTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGCCCCACGGCTTTACCGTGGCTGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaac                                                                                                                         >  2:367984/20‑249 (MQ=255)                                                                                                                           gtgccggatactctcgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCaacac                                                                                                                       <  1:175344/233‑4 (MQ=255)                                                                                                                                         cgacgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTGGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAg                                                                                                          <  1:606833/246‑1 (MQ=255)                                                                                                                                           acgTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTTACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAt                                                                                                       >  1:294721/3‑251 (MQ=255)                                                                                                                                             gTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATg                                                                                                                                                                                                                                           <  1:280686/117‑1 (MQ=255)                                                                                                                                             gTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTGCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGAtgt                                                                                                     <  1:620988/251‑1 (MQ=255)                                                                                                                                             gTATGGTTTGACTCCGGATCTACCCAATCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATg                                                                                                                                                                                                                                           >  2:280686/1‑117 (MQ=255)                                                                                                                                                   tttGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGGTGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGtgatga                                                                                                >  1:527019/1‑250 (MQ=255)                                                                                                                                                     tGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAAc                                                                                              <  1:391825/250‑1 (MQ=255)                                                                                                                                                     tGACTCCGGATCTACCCACTCTTCTGTGGTTGACGTGCGTCCTGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAAc                                                                                              <  1:394999/250‑1 (MQ=255)                                                                                                                                                      gACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACa                                                                                             >  1:290440/1‑250 (MQ=255)                                                                                                                                                       aCTCTGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACaaa                                                                                           <  2:410201/251‑1 (MQ=255)                                                                                                                                                          ccGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCGACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg                                                                                        <  1:256817/251‑1 (MQ=255)                                                                                                                                                          ccGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTg                                                                                        >  1:66592/1‑251 (MQ=255)                                                                                                                                                          ccGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGGTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACt                                                                                         <  2:151486/250‑1 (MQ=255)                                                                                                                                                                  aCCCACTCTTCTGTTGTTAACGTGCGTCCGGAATTTGCCGGTCCCGCAGCGGACATGTATCTGGAAGGTTCTGAACAGCACCGTGGTTGGTTCATGTTTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCAGTATCGTCAGGTAACGACCCACGGCTTTACCGTGGATGGTCAGGGACGCAAGATGTCTAAATCCATCGGCACCACCGTTTCGCCGAAGGATGTGATGAAAAAACTGGGAGCggg                                                                                >  2:402387/1‑250 (MQ=255)                                                                                                                                                                         cttctGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTc                                                                           <  2:168547/249‑1 (MQ=255)                                                                                                                                                                                   tgACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtgg                                                                <  1:512553/250‑1 (MQ=255)                                                                                                                                                                                    gACGTGCGTCCGGACTTTGCCGGTCACGCAGCGGACCTGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtgggg                                                              >  2:107783/1‑250 (MQ=255)                                                                                                                                                                                    gACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTgtgggt                                                              >  1:428896/1‑251 (MQ=255)                                                                                                                                                                                        tGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATTCTGCGTCTGGGGGTGGc                                                           >  2:458357/1‑250 (MQ=255)                                                                                                                                                                                          cGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATc                                                        >  2:335889/1‑251 (MQ=255)                                                                                                                                                                                           gTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATc                                                        >  1:138993/1‑250 (MQ=255)                                                                                                                                                                                           gTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAAGCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACGGGGCGCGGATAGTCTGCGTCTGTGGGTGGCATc                                                        <  2:138993/250‑1 (MQ=255)                                                                                                                                                                                                    ttGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTa                                               <  2:588770/250‑1 (MQ=255)                                                                                                                                                                                                     tGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTACCTGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGCCCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCACCCGACTacc                                             >  2:246310/1‑250 (MQ=255)                                                                                                                                                                                                            cACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCGGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGa                                      <  2:304193/251‑1 (MQ=255)                                                                                                                                                                                                              cGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGaa                                     >  1:475773/1‑250 (MQ=255)                                                                                                                                                                                                                  gCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGc                                <  1:17570/251‑1 (MQ=255)                                                                                                                                                                                                                    acacgaccgctcttccgatcttGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCTGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACGGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                               <  2:227636/229‑1 (MQ=255)                                                                                                                                                                                                                      aCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGtt                            <  2:602782/251‑1 (MQ=255)                                                                                                                                                                                                                       cATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGttt                           >  2:291096/1‑251 (MQ=255)                                                                                                                                                                                                                        aTGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTc                          >  1:197404/1‑251 (MQ=255)                                                                                                                                                                                                                          gTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTTAAATGGCCGTTTCTg                        <  2:67324/251‑1 (MQ=255)                                                                                                                                                                                                                                         tGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCTGCAAGATGTCTAAATCCATCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGcc                               >  1:227636/1‑229 (MQ=255)                                                                                                                                                                                                                                              agcacCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCCTCGGCAACACCGTTTCGCCGCAGGATGGGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGGGGCATCAACCGCCTACACCGGTGAAATGGCCGGTTCTGACGAGATCCTGAAAcacgcc    >  2:128065/3‑246 (MQ=255)                                                                                                                                                                                                                                                cacCGTGGTTGGTTCGTGTCTTCCCTAATGCTCTCCACCGCGATGAAAGGCAAAGAGCCGTATCGTCCGGTACTGACCCACGCCTTTACCGTGGATGGTCAGGGCCGCAAGTTGTCTAACTCCCTCGGCAACACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGCGGCATCAACCGCCTACACCGGCGAACTGGCCGTTTCTGACGAGATCCTGAAACGtgctgc  >  2:236620/1‑251 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   CCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAAGTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCATCGGCAATACCGTTTCGCCGCAGGATGTGATGAACAAACTGGGCGCGGATATTCTGCGTCTGTGGGTGGCATCAACCGACTACACCGGTGAAATGGCCGTTTCTGACGAGATCCTGAAACGTGCTGC  >  NC_000913/23849‑24336

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑