Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,986	T→C	D532D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,986	0	T	C	99.0%	318.3 / NA	99	D532D (GAT→GAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (37/61);  minor base A (1/0);  total (38/61)
Fisher's exact test for biased strand distribution p-value = 3.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

GAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCA  >  NC_000913/23747‑24209                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                gaggcGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGa                                                                                                                                                                                                                      <  2:490292/247‑1 (MQ=255)   gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGAATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GAATGGGAAAGAGCCGGAAACTCTC‑GACGTATGGTTTGAcc                                                                                                                                                                                                                    >  1:455627/1‑250 (MQ=255)      gATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCC‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCg                                                                                                                                                                                                                 >  2:608457/1‑251 (MQ=255)        tGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCggt                                                                                                                                                                                                               >  2:29746/1‑250 (MQ=255)         ggTTGCTAACCTTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTTCACAAAGACACGTAATAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGa                                                                                                                                                                                                               <  1:380386/250‑1 (MQ=255)         ggTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGCACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGAt                                                                                                                                                                                                              >  1:2441/1‑251 (MQ=255)                gaCCGTCCTGACTTGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGGGCCGGATACTCTC‑GACGTATGGTGTGCCTCCGGATCTAcc                                                                                                                                                                                                         <  2:233328/248‑1 (MQ=255)                aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCa                                                                                                                                                                                                       <  1:558963/251‑1 (MQ=255)                 aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTAcccgc                                                                                                                                                                                                      <  2:163967/251‑3 (MQ=255)                 aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                                                                                                                                      <  2:28648/251‑1 (MQ=255)                 aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                                                                                                                                      >  1:602782/1‑251 (MQ=255)                         gACTGGTGTATCTCCCGTCAGCGCGCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGACCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                                                                                                                              <  1:396844/251‑1 (MQ=255)                             ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCTCTCTTCTgttgtt                                                                                                                                                                                          <  2:423529/251‑1 (MQ=255)                              gtgtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGttgttg                                                                                                                                                                                         >  1:168547/1‑251 (MQ=255)                                gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGAc                                                                                                                                                                                       <  1:141238/251‑1 (MQ=255)                                     tcCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCg                                                                                                                                                                                  >  2:318720/1‑251 (MQ=255)                                         gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                                                                                              <  2:187177/251‑1 (MQ=255)                                           cAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCgg                                                                                                                                                                             <  1:185427/250‑1 (MQ=255)                                            aGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGaa                                                                                                                                                                           <  2:611940/251‑1 (MQ=255)                                               gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                                                                                                                                        <  1:240735/251‑1 (MQ=255)                                                cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg                                                                                                                                                                       <  1:128639/251‑1 (MQ=255)                                                cACCTGGGGCGTACCGATGTCACTGTTCGTGAATAAAGACACGGCAGAGCTGCATCCGCGTACCATCGAACTGATGGAAGAAGTGGCAAAAAGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCTTCGGCGCCGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTACGGTTTGACTCCGGATCTACCCACTCTCCTGTTGTTGATGTGCGTCCGGAATTTg                                                                                                                                                                       <  1:534267/251‑1 (MQ=255)                                                     ggggCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGt                                                                                                                                                                  >  1:548613/1‑251 (MQ=255)                                                         cgaaCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAAAGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                                                                                              >  2:559456/4‑251 (MQ=255)                                                           tacagATGTCACTGTGCGTGCAAAACGACACGGCAGCGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGAGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                                                                                                                             >  2:118420/5‑250 (MQ=255)                                                                  atCACTGTTCGTGCACAAAGACCCGGAAGAGCTGCACCCGCGTACCCTCGAACAGATGGAAGAAGTGGCAAAACGCGTTGCA‑GTTAACGGCATCCAGGCGCGGTGGGATCTCGATGCGAACTAGATCATCGGCGACGAAGCTGACCA‑GTACGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCAGGATCTACCCACTCTTATGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGAc                                                                                                                                                      >  2:439363/2‑250 (MQ=255)                                                                   tCACTGTTCGTGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGGCACGCAGCGGACAt                                                                                                                                                    >  1:482202/1‑251 (MQ=255)                                                                     aCTGTTCGTGCACAAAGACACGGACTAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGCA‑GTTAACGGCATCCAGGCGGGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGAGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATATACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCGGGTCACGCAGCGGACATGt                                                                                                                                                  >  2:204151/1‑251 (MQ=255)                                                                        gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTa                                                                                                                                                 <  1:470194/249‑1 (MQ=255)                                                                        gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATc                                                                                                                                               <  1:216240/251‑1 (MQ=255)                                                                            gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTgg                                                                                                                                            >  2:188176/1‑250 (MQ=255)                                                                            gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGa                                                                                                                                           <  2:505699/251‑1 (MQ=255)                                                                              gcaaaaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGTACATGTATCTGGaa                                                                                                                                          <  2:4675/246‑1 (MQ=255)                                                                              gCACAAAGACACGGAAGAGCTGCATCCTCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGaa                                                                                                                                          <  1:248619/250‑1 (MQ=255)                                                                               cacaACGACACGGAAGAACTGCATCCGAGAACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGCA‑GTTACAGGCATCCAGGCGAGGGGGGCTCTCGATGCGACAGAGATCATCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGACGGATACTCTC‑GACGTATGGTTTGACTCCGCATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTACCGGTCACACAGCGGACCAGTATCTGGAAgg                                                                                                                                        >  2:251868/1‑251 (MQ=255)                                                                                  aaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTc                                                                                                                                     <  1:417413/251‑1 (MQ=255)                                                                                   aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTTGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                                                                                                                                    <  2:548613/251‑1 (MQ=255)                                                                                         cGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCag                                                                                                                              <  2:215387/251‑2 (MQ=255)                                                                                                    cagccGCGTACCGTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAATGT‑AACGGCATCCAGGCGTGGTGGGATCTGGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                                                                                                   <  2:236904/248‑7 (MQ=255)                                                                                                    cagccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                                                                                                   <  1:329903/248‑7 (MQ=255)                                                                                                       ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGGGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATAC‑TCGCGACGTATGGTTTGACGCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACTCTGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                                                                                                 <  2:37290/250‑1 (MQ=255)                                                                                                       ccGCGTACCCGCGAACTGATGGAAGAAGTGGCAAATCGCGTTTAA‑GTTAACGGCATCCAGGCGTGTTGGTATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑TACTTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCagcatcgtggttggtt                                                                                                                <  2:465881/251‑16 (MQ=255)                                                                                                            tACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                                                                                                           >  2:346732/1‑251 (MQ=255)                                                                                                                  cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTGAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                                                                                                     <  1:608457/250‑1 (MQ=255)                                                                                                                        gATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCTGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAAt                                                                                                 <  2:531295/249‑1 (MQ=255)                                                                                                                         aTGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAt                                                                                              >  1:135093/1‑251 (MQ=255)                                                                                                                            gaagaaGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtctc                                                                                           <  1:536829/251‑1 (MQ=255)                                                                                                                            gaagaaGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtctc                                                                                           >  1:488677/1‑251 (MQ=255)                                                                                                                              agaagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTTCCGGATACGCTC‑GACGTATGTTTTGACTCCGGATCTACCGACTCGTCTGTTGTTGACGTGCGTCCGTAATGTGCCGGTTGTGCAGCGGACATGTATCTGTAATGTTCTGACCAGCGCCGTGGGTGGGGCATGTCTTCCCGAATGATCTCCa                                                                                         <  2:64937/251‑1 (MQ=255)                                                                                                                              agaagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTACATGTCTTCCCTAATGATCTCCa                                                                                         <  1:168967/251‑1 (MQ=255)                                                                                                                              agaagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGGGGTGGGATCGCGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCa                                                                                         <  2:548485/251‑1 (MQ=255)                                                                                                                                aagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTCATGATCTCCAcc                                                                                       >  2:379053/1‑251 (MQ=255)                                                                                                                                  gTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgc                                                                                     >  1:263487/1‑251 (MQ=255)                                                                                                                                  gTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCCCcgc                                                                                     >  2:492670/1‑251 (MQ=255)                                                                                                                                   gggCAAAACGCGTGGAA‑GTTAACGGCATCCCGGCGTGGTGGGATCTCGAGGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgct                                                                                    <  2:335793/250‑2 (MQ=255)                                                                                                                                    ggCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTc                                                                                                               <  1:532055/223‑1 (MQ=255)                                                                                                                                    ggCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcg                                                                                    <  1:602125/250‑1 (MQ=255)                                                                                                                                    ggCAAAACGCGTTGAA‑GTTAAAGGCATCCAGGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAAGCTGACCC‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACAAGCACCGTGGTTGGTTc                                                                                                               >  2:532055/1‑223 (MQ=255)                                                                                                                                      cAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcgctg                                                                                 >  1:304193/1‑248 (MQ=255)                                                                                                                                       aaaaCGCGTAGAA‑GTTAACGGCATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGA‑CACGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcgctg                                                                                 >  2:430957/1‑247 (MQ=255)                                                                                                                                        aaaCGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa                                                                               <  1:29746/251‑1 (MQ=255)                                                                                                                                                       taacggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCg                                                                  <  1:108158/246‑1 (MQ=255)                                                                                                                                                        aacggCATCCAGGCGTGGTGGGAGCTCGATGCGAAAGAGAGCCTCGGCGACGAAGCTTACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTCGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTa                                                                <  2:144663/248‑1 (MQ=255)                                                                                                                                                         acggCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTAt                                                               >  1:21136/3‑251 (MQ=255)                                                                                                                                                             cATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTc                                                           <  1:118420/251‑1 (MQ=255)                                                                                                                                                              aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCa                                                          <  1:21294/251‑1 (MQ=255)                                                                                                                                                              aTCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGCGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTGGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCTCCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGTGCCGTATCGTCa                                                          <  2:585550/251‑1 (MQ=255)                                                                                                                                                              aTCCAGGCGTGGTCGGATCTCGATGCGACAGTGCTCCTCGGCGAAGAAGCTGCCCA‑GCATGTGCGAGGCCCGGATCCTCTC‑GACGTATGGTTTGACTCCGGATCTACACACTCTTCTGTTGTTGACGTGCGTCAGGAATTTACCGGTCACGCAGCGGACATGTATCTGGCAGGTTCTGACCCGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAACGGCACAGCGCCGTATCGTcc                                                          >  2:365584/1‑250 (MQ=255)                                                                                                                                                                  aGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGt                                                       >  1:588770/1‑250 (MQ=255)                                                                                                                                                                   ggCGTGGTGGTATCTCGAGGCGAAAGAGAGCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGCCCGGAAGTTGGCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGCCCAGAACCGTGGTTGGGTCATGTCTTTCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTAc                                                     <  1:362584/251‑1 (MQ=255)                                                                                                                                                                     cGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTg                                                   >  1:458880/1‑251 (MQ=255)                                                                                                                                                                         gtggGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGAccc                                               <  2:488677/251‑1 (MQ=255)                                                                                                                                                                         gtggGATCTCGATGCGAAAGAGATACTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGCTCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGACAGGCAAAGCGACGTCTCGTCAGGTACTGAccc                                               >  2:582385/1‑251 (MQ=255)                                                                                                                                                                            ggATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACg                                            <  1:209993/251‑1 (MQ=255)                                                                                                                                                                              aTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGc                                          >  2:61464/1‑251 (MQ=255)                                                                                                                                                                               tctcGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCTCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCt                                         >  1:128873/1‑251 (MQ=255)                                                                                                                                                                                  cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCttt                                       <  1:486453/250‑1 (MQ=255)                                                                                                                                                                                  cGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCGGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACgg                                           <  1:418771/246‑1 (MQ=255)                                                                                                                                                                                  cGATGCGAAAGAGAGCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTAGCTGGAAGGTTCTGACCAGCACCGTGGTTGGGTCATGTCTTCCCTAATGATCGCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACgg                                           <  2:275894/246‑1 (MQ=255)                                                                                                                                                                                       cGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTg                                 <  1:10049/251‑1 (MQ=255)                                                                                                                                                                                         aaaGAGATCCACGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTgg                                >  1:478061/1‑250 (MQ=255)                                                                                                                                                                                          aaGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGAt                              <  1:1368/251‑1 (MQ=255)                                                                                                                                                                                              gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                          <  1:584206/251‑1 (MQ=255)                                                                                                                                                                                              gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                          >  2:143899/1‑251 (MQ=255)                                                                                                                                                                                              gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAAGGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGTCCCACGGCTTTACCGTGGATGGTc                          <  1:57253/251‑1 (MQ=255)                                                                                                                                                                                              gaTCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGGATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTc                          >  1:515005/1‑251 (MQ=255)                                                                                                                                                                                                 ccTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAgg                       >  1:11777/1‑251 (MQ=255)                                                                                                                                                                                                    cGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                    <  1:456232/251‑1 (MQ=255)                                                                                                                                                                                                    cGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGcc                    >  1:330591/1‑251 (MQ=255)                                                                                                                                                                                                     ggCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GAAGTATGGTTTGACTCCGGATCTACCCACTCTTATGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                   >  1:32042/1‑251 (MQ=255)                                                                                                                                                                                                     ggCGACGAAGCTGACCA‑GGATGTGAAAGTGCCGGATACTCTC‑GACGTATGGGTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCg                   <  2:60828/251‑1 (MQ=255)                                                                                                                                                                                                       cgacgaAGCTGACCA‑GTATGTGAAAGTGCCGGATTCTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                 <  2:129163/251‑1 (MQ=255)                                                                                                                                                                                                        gacgaAGCTGACCA‑GTATGTGACAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCa                 >  2:279765/1‑250 (MQ=255)                                                                                                                                                                                                          ggaAGCTGACCA‑GTATGTGAAATTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTGCTGTTGGTGACGTGCGTCCGGAATTTGCCGGTCACGCAGGGGACAGGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTGATGATCTCCACCGCGATGGAAGGCAAAGCGCCGTATCGTCAGGTACGGACCCACGGCTTTACCTTGGATGGTCATGGCCGCAAGa              <  2:281514/250‑1 (MQ=255)                                                                                                                                                                                                          cgaAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGa              <  1:87783/251‑1 (MQ=255)                                                                                                                                                                                                           gaAGCTGACCA‑GTATGTGAAAGTGCCGGATAC‑TCGCGACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGAt             <  2:2441/251‑1 (MQ=255)                                                                                                                                                                                                                 gaccA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCt         <  2:73093/246‑1 (MQ=255)                                                                                                                                                                                                                       gtatgTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCa  <  2:515005/247‑1 (MQ=255)                                                                                                                                                                                                                       gtatgTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAAGGCAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCa  <  1:442099/247‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                GAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAAGGGTAAAGCGCCGTATCGTCAGGTACTGACCCACGGCTTTACCGTGGATGGTCAGGGCCGCAAGATGTCTAAATCCA  >  NC_000913/23747‑24209

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑