BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,899	T→C	D503D (GAT→GAC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,899	0	T	C	98.2%	359.8 / NA	110	D503N (GAT→AAC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (41/67); minor base A (2/0); total (43/67)
Fisher's exact test for biased strand distribution p-value = 1.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 110 total aligned reads displayed.

CAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAA  >  NC_000913/23659‑24132

cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAgg                                                                                                                                                                                                                                   <  2:168557/251‑1 (MQ=255)

 aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGc                                                                                                                                                                                                                                  >  2:584206/1‑251 (MQ=255)

    aTGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGTCGATGGTTGCTAACCGTCCGGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGGCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCgtg                                                                                                                                                                                                                               <  1:360696/251‑1 (MQ=255)

       gATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACTGCATCCAGGCgtggtg                                                                                                                                                                                                                            <  2:43393/251‑1 (MQ=255)

        aTCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGtggtgg                                                                                                                                                                                                                           <  2:446400/251‑1 (MQ=255)

          cAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGa                                                                                                                                                                                                                         <  1:459354/251‑1 (MQ=255)

          cAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTACTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTgggg                                                                                                                                                                                                                         >  2:248619/1‑250 (MQ=255)

           aGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGAt                                                                                                                                                                                                                        >  2:275779/1‑251 (MQ=255)

               aGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCg                                                                                                                                                                                                                    <  1:487594/251‑1 (MQ=255)

                     gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGa                                                                                                                                                                                                              <  1:559268/251‑1 (MQ=255)

                     gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCg                                                                                                                                                                                                               <  1:275779/250‑1 (MQ=255)

                      cgtgcgCAGTCACGGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGTCGATGGTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGGGGGATCTCGATGCGaa                                                                                                                                                                                                             <  2:278278/251‑1 (MQ=255)

                             aGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATc                                                                                                                                                                                                      <  1:313285/251‑1 (MQ=255)

                               tCACTGAAAGAGGTAAACGGCGTGCCGTGGATCCCGGACTGGCGCCAGGCGCGTATCGAGTCGATGGATGCTAACAGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCCCAAAGACACGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAAGACGTGGCAACATGCGTTGAA‑GTTAACGGCATCCAGGAGTGATGGGAACTCGATGCGAAagagtcccc                                                                                                                                                                                                    >  2:199144/1‑246 (MQ=255)

                                cccTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGTCGCGTAGCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATACTc                                                                                                                                                                                                   <  2:173782/249‑1 (MQ=255)

                                 acggAAAGAGATCAAAGGCGTGCAGTGGATCCCGGCCTGGGGCCAGGCGCGTATCGAGTCGATGGTTTCTAACCGGCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACACTCGAACTGATGGAAGAAGTGGCAAAACGCGTGGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCg                                                                                                                                                                                                  <  2:63639/248‑1 (MQ=255)

                                   agAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATGTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGc                                                                                                                                                                                                >  2:153313/2‑251 (MQ=255)

                                     aaaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcga                                                                                                                                                                                              <  1:373196/251‑1 (MQ=255)

                                      aaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgac                                                                                                                                                                                             <  1:519533/251‑1 (MQ=255)

                                      aaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgac                                                                                                                                                                                             <  1:145065/251‑1 (MQ=255)

                                             cAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt                                                                                                                                                                                       <  1:301991/250‑1 (MQ=255)

                                             cAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGGTGAA‑GTTAACGGCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt                                                                                                                                                                                       <  2:606711/250‑1 (MQ=255)

                                             cAAAGGCGTGCAGTGGATCAAGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGCATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGCGGCAAAACGCGGTGAA‑GGTAACGGCATCCAGGCATGGGGGGCTCTCGATGCGAAAGAGATCCTCGGcgacgagcctg                                                                                                                                                                                      >  2:456232/1‑246 (MQ=255)

                                               aaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGac                                                                                                                                                                                    <  1:238642/251‑2 (MQ=255)

                                               aaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGac                                                                                                                                                                                    <  1:442692/251‑2 (MQ=255)

                                                    gTGCAGTGGCTCCAGGACTGGGGCCGGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGCCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGATGAAGTGACAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑Gt                                                                                                                                                                               >  2:606833/1‑250 (MQ=255)

                                                     tGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTat                                                                                                                                                                             >  2:470194/1‑250 (MQ=255)

                                                      gCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTAGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTat                                                                                                                                                                             <  2:34038/250‑2 (MQ=255)

                                                        aGTGGATCCCGGACTGAGGACAGGCGCGTATCGCGTCGCTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGAGGCACAACGCGTTGAA‑GTTAACGGCATCCAGGAGCGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGACGCTGacccgactgtg                                                                                                                                                                           >  2:561758/1‑241 (MQ=255)

                                                         gTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGa                                                                                                                                                                         <  1:235833/251‑1 (MQ=255)

                                                          tGGATCCCGGACTGGGGACAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACACTCGTACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAAGCTGAACA‑GTATGTGa                                                                                                                                                                         >  2:370187/1‑250 (MQ=255)

                                                           ggATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGaaa                                                                                                                                                                       <  1:554234/251‑1 (MQ=255)

                                                               cccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTg                                                                                                                                                                    <  1:261303/250‑1 (MQ=255)

                                                                  ggACGGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGGGCCgg                                                                                                                                                                <  2:38772/251‑1 (MQ=255)

                                                                   gACTGGGGCCAGGCGCGTATCGAGTCGCTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAAAAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGCTGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCggc                                                                                                                                                               >  2:256817/1‑250 (MQ=255)

                                                                      tGGGGCCAGGCGCGTATCGCGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatac                                                                                                                                                            >  1:275894/1‑248 (MQ=255)

                                                                       ggggCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatact                                                                                                                                                           >  1:158897/1‑247 (MQ=255)

                                                                        gggCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAATAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactc                                                                                                                                                          <  1:603305/251‑6 (MQ=255)

                                                                        gggCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactc                                                                                                                                                          <  2:509509/251‑6 (MQ=255)

                                                                          gCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTTACCA‑GTATGTGAAAGTGCCGGatactctc                                                                                                                                                        <  1:189446/251‑8 (MQ=255)

                                                                             aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactctcgac                                                                                                                                                     >  1:389921/1‑241 (MQ=255)

                                                                             aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGaaactctcgac                                                                                                                                                     >  1:531295/1‑241 (MQ=255)

                                                                               gcgcGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactctcgacgt                                                                                                                                                   <  2:137939/251‑13 (MQ=255)

                                                                                cgcgTAGCGAGTCGAGGGTTGCTAACCGTCCGTACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAATCTGTCCA‑GTATGTGAAAGTGCCGGacacgctcgacgta                                                                                                                                                  <  2:301145/251‑11 (MQ=255)

                                                                                  cgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGAAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGatactctcgacgtatg                                                                                                                                                >  1:129163/1‑236 (MQ=255)

                                                                                      tCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCCGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAACGACACGGCAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGCTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGCAGCTGACCA‑GTATGTGAAAGTGCCGGatactctcgacgtatggttt                                                                                                                                            >  1:318034/1‑232 (MQ=255)

                                                                                        gaggcGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGa                                                                                                                                         <  2:490292/247‑1 (MQ=255)

                                                                                          gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGAATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GAATGGGAAAGAGCCGGAAACTCTC‑GACGTATGGTTTGAcc                                                                                                                                       >  1:455627/1‑250 (MQ=255)

                                                                                             gATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCC‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCg                                                                                                                                    >  2:608457/1‑251 (MQ=255)

                                                                                               tGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCggt                                                                                                                                  >  2:29746/1‑250 (MQ=255)

                                                                                                ggTTGCTAACCTTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTTCACAAAGACACGTAATAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGa                                                                                                                                  <  1:380386/250‑1 (MQ=255)

                                                                                                ggTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGCACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGAt                                                                                                                                 >  1:2441/1‑251 (MQ=255)

                                                                                                       gaCCGTCCTGACTTGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGGGCCGGATACTCTC‑GACGTATGGTGTGCCTCCGGATCTAcc                                                                                                                            <  2:233328/248‑1 (MQ=255)

                                                                                                       aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCa                                                                                                                          <  1:558963/251‑1 (MQ=255)

                                                                                                        aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTAcccgc                                                                                                                         <  2:163967/251‑3 (MQ=255)

                                                                                                        aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                                                         >  1:602782/1‑251 (MQ=255)

                                                                                                        aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                                                         <  2:28648/251‑1 (MQ=255)

                                                                                                                gACTGGTGTATCTCCCGTCAGCGCGCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGACCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                                                 <  1:396844/251‑1 (MQ=255)

                                                                                                                    ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCTCTCTTCTgttgtt                                                                                                             <  2:423529/251‑1 (MQ=255)

                                                                                                                     gtgtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGttgttg                                                                                                            >  1:168547/1‑251 (MQ=255)

                                                                                                                       gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGAc                                                                                                          <  1:141238/251‑1 (MQ=255)

                                                                                                                                gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                                                 <  2:187177/251‑1 (MQ=255)

                                                                                                                                  cAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCgg                                                                                                <  1:185427/250‑1 (MQ=255)

                                                                                                                                   aGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGaa                                                                                              <  2:611940/251‑1 (MQ=255)

                                                                                                                                      gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                                                           <  1:240735/251‑1 (MQ=255)

                                                                                                                                       cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg                                                                                          <  1:128639/251‑1 (MQ=255)

                                                                                                                                            ggggCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGt                                                                                     >  1:548613/1‑251 (MQ=255)

                                                                                                                                                cgaaCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAAAGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                                                 >  2:559456/4‑251 (MQ=255)

                                                                                                                                                  tacagATGTCACTGTGCGTGCAAAACGACACGGCAGCGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGAGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                                                >  2:118420/5‑250 (MQ=255)

                                                                                                                                                          tCACTGTTCGTGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGGCACGCAGCGGACAt                                                                       >  1:482202/1‑251 (MQ=255)

                                                                                                                                                            aCTGTTCGTGCACAAAGACACGGACTAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGCA‑GTTAACGGCATCCAGGCGGGGAGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGAGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATATACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCGGGTCACGCAGCGGACATGt                                                                     >  2:204151/1‑251 (MQ=255)

                                                                                                                                                               gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTa                                                                    <  1:470194/249‑1 (MQ=255)

                                                                                                                                                                   gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTgg                                                               >  2:188176/1‑250 (MQ=255)

                                                                                                                                                                   gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGa                                                              <  2:505699/251‑1 (MQ=255)

                                                                                                                                                                     gcaaaaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGTACATGTATCTGGaa                                                             <  2:4675/246‑1 (MQ=255)

                                                                                                                                                                     gCACAAAGACACGGAAGAGCTGCATCCTCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGaa                                                             <  1:248619/250‑1 (MQ=255)

                                                                                                                                                                      cacaACGACACGGAAGAACTGCATCCGAGAACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGCA‑GTTACAGGCATCCAGGCGAGGGGGGCTCTCGATGCGACAGAGATCATCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGACGGATACTCTC‑GACGTATGGTTTGACTCCGCATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTACCGGTCACACAGCGGACCAGTATCTGGAAgg                                                           >  2:251868/1‑251 (MQ=255)

                                                                                                                                                                          aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTTGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                                                       <  2:548613/251‑1 (MQ=255)

                                                                                                                                                                                cGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCag                                                 <  2:215387/251‑2 (MQ=255)

                                                                                                                                                                                           cagccGCGTACCGTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAATGT‑AACGGCATCCAGGCGTGGTGGGATCTGGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                      <  2:236904/248‑7 (MQ=255)

                                                                                                                                                                                           cagccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg                                      <  1:329903/248‑7 (MQ=255)

                                                                                                                                                                                              ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGGGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATAC‑TCGCGACGTATGGTTTGACGCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACTCTGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt                                    <  2:37290/250‑1 (MQ=255)

                                                                                                                                                                                              ccGCGTACCCGCGAACTGATGGAAGAAGTGGCAAATCGCGTTTAA‑GTTAACGGCATCCAGGCGTGTTGGTATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑TACTTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCagcatcgtggttggtt                                   <  2:465881/251‑16 (MQ=255)

                                                                                                                                                                                                   tACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGCCATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGt                              >  2:346732/1‑251 (MQ=255)

                                                                                                                                                                                                         cgAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTGAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTccc                        <  1:608457/250‑1 (MQ=255)

                                                                                                                                                                                                               gATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCTGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGGCTTCCCTAAt                    <  2:531295/249‑1 (MQ=255)

                                                                                                                                                                                                                aTGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAt                 >  1:135093/1‑251 (MQ=255)

                                                                                                                                                                                                                   gaagaaGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtctc              <  1:536829/251‑1 (MQ=255)

                                                                                                                                                                                                                   gaagaaGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGAtctc              >  1:488677/1‑251 (MQ=255)

                                                                                                                                                                                                                     agaagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTACATGTCTTCCCTAATGATCTCCa            <  1:168967/251‑1 (MQ=255)

                                                                                                                                                                                                                     agaagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGGGGTGGGATCGCGATGCGAAAGAGATCCGCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACGCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCa            <  2:548485/251‑1 (MQ=255)

                                                                                                                                                                                                                       aagTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTCATGATCTCCAcc          >  2:379053/1‑251 (MQ=255)

                                                                                                                                                                                                                         gTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgc        >  1:263487/1‑251 (MQ=255)

                                                                                                                                                                                                                         gTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGAACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCCCcgc        >  2:492670/1‑251 (MQ=255)

                                                                                                                                                                                                                          gggCAAAACGCGTGGAA‑GTTAACGGCATCCCGGCGTGGTGGGATCTCGAGGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgct       <  2:335793/250‑2 (MQ=255)

                                                                                                                                                                                                                           ggCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTc                                  <  1:532055/223‑1 (MQ=255)

                                                                                                                                                                                                                           ggCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcg       <  1:602125/250‑1 (MQ=255)

                                                                                                                                                                                                                             cAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcgctg    >  1:304193/1‑248 (MQ=255)

                                                                                                                                                                                                                              aaaaCGCGTAGAA‑GTTAACGGCATCCAGGCGTGGTGGGCTCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGA‑CACGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACcgcgctg    >  2:430957/1‑247 (MQ=255)

                                                                                                                                                                                                                               aaaCGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCA‑GTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGTGGTTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGaa  <  1:29746/251‑1 (MQ=255)

CAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCA‑GTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGTCTTCCCTAATGATCTCCACCGCGATGAA  >  NC_000913/23659‑24132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`