BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,860	T→C	L490L (CTT→CTC)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,860	0	T	C	100.0%	371.5 / NA	114	L490L (CTT→CTC)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (48/66); total (48/66)

Only 100 of 114 total aligned reads displayed.

CACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGT  >  NC_000913/23615‑24104

cacaAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCCAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACCAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACt                                                                                                                                                                                                                                                   >  2:602125/1‑251 (MQ=255)

  caAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTg                                                                                                                                                                                                                                                  >  2:373196/1‑250 (MQ=255)

   aaaaCGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGAt                                                                                                                                                                                                                                                >  1:28648/1‑251 (MQ=255)

      aCGCCGATCATCTTCCGCGCTACGCCGCAGGGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCTACGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGAGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCTCGTACCCTCTAACTGATGga                                                                                                                                                                                                                                             <  1:151588/251‑1 (MQ=255)

        gCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACCAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                                                                             >  1:151486/1‑249 (MQ=255)

         ccGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                                                                             >  1:144663/1‑248 (MQ=255)

         ccGATAATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAACGGCGTGCAGAGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGga                                                                                                                                                                                                                                             >  2:50343/1‑248 (MQ=255)

            atcatcTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGaagaagg                                                                                                                                                                                                                                       >  1:73093/1‑250 (MQ=255)

              catcTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGAGGGAAGAAGTgg                                                                                                                                                                                                                                     <  2:448719/251‑1 (MQ=255)

                tcTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGc                                                                                                                                                                                                                                    >  2:175344/1‑250 (MQ=255)

                    ccGCGCGACGCCGCAGTGGTTCGTCAGCATGGCACAGAACGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCAACTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGATGAGCTGCATCCGCGTACCCTCGACCTGTTGGAAGAAGAGGCaagac                                                                                                                                                                                                                               >  2:353762/1‑248 (MQ=255)

                     cgcgcgACGCCGCAGCGGTACGTCAGCTTGGATCAGAAAGGTCTGCGCGCGCAGTCACTGCAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGACGCGCTGCATCCGCGTCCCCTCGAACTGATGGAAGAAGTggaaacacg                                                                                                                                                                                                                              >  2:620988/1‑244 (MQ=255)

                        gcgACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAACGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTAACCTCGAACTGATGGAAGGAGTGGCAAAACGCGt                                                                                                                                                                                                                           >  2:517584/1‑251 (MQ=255)

                         cggcgctgcAGTGGTTCGTCAGCATGGAGCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGCTCCTGGACTGGGGCCAGGGGCGTAGCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGCCAGCGCACCTGGGGCGTACCGATGTCGCTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGtt                                                                                                                                                                                                                          <  1:354957/244‑1 (MQ=255)

                          gACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTg                                                                                                                                                                                                                         <  1:438064/251‑1 (MQ=255)

                              ccgcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACAGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGGAGCGCCCGTCAGCGCACGTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAATAAGTGGCTAAAGGCGTTGAA‑Gt                                                                                                                                                                                                                    <  2:281969/251‑1 (MQ=255)

                                gcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑Gtta                                                                                                                                                                                                                  <  1:474297/251‑3 (MQ=255)

                                gcAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGAACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑Gtt                                                                                                                                                                                                                   >  1:410201/1‑249 (MQ=255)

                                        tcggcAGCATGGATCAGAAAGGTCTGCGTGCGAAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑Gttaacggcat                                                                                                                                                                                                           <  1:385794/246‑10 (MQ=255)

                                         cGTCAGCATGGATCAGAAAGGTCTGCGTGCGCTGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGGCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑Gttaacggcatc                                                                                                                                                                                                          <  2:478986/250‑11 (MQ=255)

                                            cAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAgg                                                                                                                                                                                                      <  2:168557/251‑1 (MQ=255)

                                             aGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGc                                                                                                                                                                                                     >  2:584206/1‑251 (MQ=255)

                                                aTGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGTCGATGGTTGCTAACCGTCCGGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGGCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCgtg                                                                                                                                                                                                  <  1:360696/251‑1 (MQ=255)

                                                   gATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACTGCATCCAGGCgtggtg                                                                                                                                                                                               <  2:43393/251‑1 (MQ=255)

                                                    aTCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGtggtgg                                                                                                                                                                                              <  2:446400/251‑1 (MQ=255)

                                                     tCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTgggggg                                                                                                                                                                                             >  2:216240/1‑251 (MQ=255)

                                                      cAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGa                                                                                                                                                                                            <  1:459354/251‑1 (MQ=255)

                                                      cAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTACTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTgggg                                                                                                                                                                                            >  2:248619/1‑250 (MQ=255)

                                                           aGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCg                                                                                                                                                                                       <  1:487594/251‑1 (MQ=255)

                                                                 gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCCCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGa                                                                                                                                                                                 <  1:559268/251‑1 (MQ=255)

                                                                 gcgtgcgCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCg                                                                                                                                                                                  <  1:275779/250‑1 (MQ=255)

                                                                  cgtgcgCAGTCACGGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTAGCGAGTCGATGGTTGCTACCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGGGGGATCTCGATGCGaa                                                                                                                                                                                <  2:278278/251‑1 (MQ=255)

                                                                         aGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATc                                                                                                                                                                         <  1:313285/251‑1 (MQ=255)

                                                                           tCACTGAAAGAGGTAAACGGCGTGCCGTGGATCCCGGACTGGCGCCAGGCGCGTATCGAGTCGATGGATGCTAACAGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCCCAAAGACACGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAAGACGTGGCAACATGCGTTGAA‑GTTAACGGCATCCAGGAGTGATGGGAACTCGATGCGAAagagtcccc                                                                                                                                                                       >  2:199144/1‑246 (MQ=255)

                                                                            cccTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGTCGCGTAGCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATACTc                                                                                                                                                                      <  2:173782/249‑1 (MQ=255)

                                                                               agAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATGTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGc                                                                                                                                                                   >  2:153313/2‑251 (MQ=255)

                                                                                 aaaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcga                                                                                                                                                                 <  1:373196/251‑1 (MQ=255)

                                                                                  aaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgac                                                                                                                                                                <  1:519533/251‑1 (MQ=255)

                                                                                  aaGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGcgac                                                                                                                                                                <  1:145065/251‑1 (MQ=255)

                                                                                         cAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt                                                                                                                                                          <  1:301991/250‑1 (MQ=255)

                                                                                         cAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGGTGAA‑GTTAACGGCATCCAGGCGTGGTTGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCt                                                                                                                                                          <  2:606711/250‑1 (MQ=255)

                                                                                         cAAAGGCGTGCAGTGGATCAAGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGCATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGCGGCAAAACGCGGTGAA‑GGTAACGGCATCCAGGCATGGGGGGCTCTCGATGCGAAAGAGATCCTCGGcgacgagcctg                                                                                                                                                         >  2:456232/1‑246 (MQ=255)

                                                                                           aaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGac                                                                                                                                                       <  1:442692/251‑2 (MQ=255)

                                                                                           aaGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGac                                                                                                                                                       <  1:238642/251‑2 (MQ=255)

                                                                                                gTGCAGTGGCTCCAGGACTGGGGCCGGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGCCTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGATGAAGTGACAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGt                                                                                                                                                   >  2:606833/1‑250 (MQ=255)

                                                                                                 tGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTat                                                                                                                                                 >  2:470194/1‑250 (MQ=255)

                                                                                                  gCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCGCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTAGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTat                                                                                                                                                 <  2:34038/250‑2 (MQ=255)

                                                                                                    aGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCAATGTCACTGTTCGTGCACAAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAACGAGATCCTCGGCGACGAAGCTGACCAGTAtgtg                                                                                                                                              >  1:301145/1‑251 (MQ=255)

                                                                                                    aGTGGATCCCGGACTGAGGACAGGCGCGTATCGCGTCGCTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGCACTGATGGAAGAAGAGGCACAACGCGTTGAA‑GTTAACGGCATCCAGGAGCGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGACGCTGacccgactgtg                                                                                                                                              >  2:561758/1‑241 (MQ=255)

                                                                                                     gTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGa                                                                                                                                             <  1:235833/251‑1 (MQ=255)

                                                                                                      tGGATCCCGGACTGGGGACAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACACTCGTACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGCAAGAGATCCTCGGCGACGAAGCTGAACAGTATGTGa                                                                                                                                             >  2:370187/1‑250 (MQ=255)

                                                                                                           cccGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTg                                                                                                                                        <  1:261303/250‑1 (MQ=255)

                                                                                                              ggACGGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCgg                                                                                                                                    <  2:38772/251‑1 (MQ=255)

                                                                                                               gACTGGGGCCAGGCGCGTATCGAGTCGCTGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCAAAAAGACACGGACGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGCTGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCggc                                                                                                                                   >  2:256817/1‑250 (MQ=255)

                                                                                                                  tGGGGCCAGGCGCGTATCGCGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatac                                                                                                                                >  1:275894/1‑248 (MQ=255)

                                                                                                                   ggggCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatact                                                                                                                               >  1:158897/1‑247 (MQ=255)

                                                                                                                    gggCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAATAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactc                                                                                                                              <  1:603305/251‑6 (MQ=255)

                                                                                                                    gggCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactc                                                                                                                              <  2:509509/251‑6 (MQ=255)

                                                                                                                      gCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTTACCAGTATGTGAAAGTGCCGGatactctc                                                                                                                            <  1:189446/251‑8 (MQ=255)

                                                                                                                       ccAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcg                                                                                                                           <  2:129424/251‑9 (MQ=255)

                                                                                                                         aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgac                                                                                                                         >  1:389921/1‑241 (MQ=255)

                                                                                                                         aGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGaaactctcgac                                                                                                                         >  1:531295/1‑241 (MQ=255)

                                                                                                                            cgcgTAGCGAGTCGAGGGTTGCTAACCGTCCGTACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCAGCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAATCTGTCCAGTATGTGAAAGTGCCGGacacgctcgacgta                                                                                                                      <  2:301145/251‑11 (MQ=255)

                                                                                                                              cgTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGAAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGatactctcgacgtatg                                                                                                                    >  1:129163/1‑236 (MQ=255)

                                                                                                                                      gTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGAATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGAATGGGAAAGAGCCGGAAACTCTC‑GACGTATGGTTTGAcc                                                                                                           >  1:455627/1‑250 (MQ=255)

                                                                                                                                         gATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGCCACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCCGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCg                                                                                                        >  2:608457/1‑251 (MQ=255)

                                                                                                                                           tGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCggt                                                                                                      >  2:29746/1‑250 (MQ=255)

                                                                                                                                            ggTTGCTAACCTTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTTCACAAAGACACGTAATAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGa                                                                                                      <  1:380386/250‑1 (MQ=255)

                                                                                                                                            ggTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGCACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGAt                                                                                                     >  1:2441/1‑251 (MQ=255)

                                                                                                                                                   gaCCGTCCTGACTTGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGGGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGGGCCGGATACTCTC‑GACGTATGGTGTGCCTCCGGATCTAcc                                                                                                <  2:233328/248‑1 (MQ=255)

                                                                                                                                                   aaCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCa                                                                                              <  1:558963/251‑1 (MQ=255)

                                                                                                                                                    aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTAcccgc                                                                                             <  2:163967/251‑3 (MQ=255)

                                                                                                                                                    aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                             >  1:602782/1‑251 (MQ=255)

                                                                                                                                                    aCCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCAc                                                                                             <  2:28648/251‑1 (MQ=255)

                                                                                                                                                            gACTGGTGTATCTCCCGTCAGCGCGCCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTCCCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGACCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCtgt                                                                                     <  1:396844/251‑1 (MQ=255)

                                                                                                                                                                ggTGTATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCTCTCTTCTgttgtt                                                                                 <  2:423529/251‑1 (MQ=255)

                                                                                                                                                                   gtATCTCCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGAc                                                                              <  1:141238/251‑1 (MQ=255)

                                                                                                                                                                        tcCCGTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCg                                                                         >  2:318720/1‑251 (MQ=255)

                                                                                                                                                                            gTCAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCg                                                                     <  2:187177/251‑1 (MQ=255)

                                                                                                                                                                              cAGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCgg                                                                    <  1:185427/250‑1 (MQ=255)

                                                                                                                                                                               aGCGCACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGaa                                                                  <  2:611940/251‑1 (MQ=255)

                                                                                                                                                                                  gcACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAAttt                                                               <  1:240735/251‑1 (MQ=255)

                                                                                                                                                                                   cACCTGGGGCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTg                                                              <  1:128639/251‑1 (MQ=255)

                                                                                                                                                                                   cACCTGGGGCGTACCGATGTCACTGTTCGTGAATAAAGACACGGCAGAGCTGCATCCGCGTACCATCGAACTGATGGAAGAAGTGGCAAAAAGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCTTCGGCGCCGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTACGGTTTGACTCCGGATCTACCCACTCTCCTGTTGTTGATGTGCGTCCGGAATTTg                                                              <  1:534267/251‑1 (MQ=255)

                                                                                                                                                                                        ggggCGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGt                                                         >  1:548613/1‑251 (MQ=255)

                                                                                                                                                                                            cgaaCCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAAAGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACg                                                     >  2:559456/4‑251 (MQ=255)

                                                                                                                                                                                              tacagATGTCACTGTGCGTGCAAAACGACACGGCAGCGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGAGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGc                                                    >  2:118420/5‑250 (MQ=255)

                                                                                                                                                                                                      tCACTGTTCGTGCACAAAGACCCGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGGCACGCAGCGGACAt                                           >  1:482202/1‑251 (MQ=255)

                                                                                                                                                                                                           gTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATc                                      <  1:216240/251‑1 (MQ=255)

                                                                                                                                                                                                               gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGCCCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTgg                                   >  2:188176/1‑250 (MQ=255)

                                                                                                                                                                                                               gTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGa                                  <  2:505699/251‑1 (MQ=255)

                                                                                                                                                                                                                 gcaaaaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACGGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCACGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGTACATGTATCTGGaa                                 <  2:4675/246‑1 (MQ=255)

                                                                                                                                                                                                                  cacaACGACACGGAAGAACTGCATCCGAGAACCCTCGAACTGCTGGAAGAAGTGGCAAAACGCGTTGCA‑GTTACAGGCATCCAGGCGAGGGGGGCTCTCGATGCGACAGAGATCATCGGCGACGAAGCTGACCAGTATGTGAAAGTGACGGATACTCTC‑GACGTATGGTTTGACTCCGCATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTACCGGTCACACAGCGGACCAGTATCTGGAAgg                               >  2:251868/1‑251 (MQ=255)

                                                                                                                                                                                                                     aaaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTc                            <  1:417413/251‑1 (MQ=255)

                                                                                                                                                                                                                      aaGACACGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTTGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCt                           <  2:548613/251‑1 (MQ=255)

                                                                                                                                                                                                                            cGGAAGAGCTGCATCCGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCag                     <  2:215387/251‑2 (MQ=255)

                                                                                                                                                                                                                                       cagccGCGTACCGTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAATGT‑AACGGCATCCAGGCGTGGTGGGATCTGGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg          <  2:236904/248‑7 (MQ=255)

                                                                                                                                                                                                                                       cagccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑GACGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAGCACcgtggttg          <  1:329903/248‑7 (MQ=255)

                                                                                                                                                                                                                                          ccGCGTACCCTCGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTTAACGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGGGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATAC‑TCGCGACGTATGGTTTGACGCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACTCTGCGGACATGTATCTGGAAGGTTCTGACCAGCACCGtggttggt        <  2:37290/250‑1 (MQ=255)

                                                                                                                                                                                                                                          ccGCGTACCCGCGAACTGATGGAAGAAGTGGCAAATCGCGTTTAA‑GTTAACGGCATCCAGGCGTGTTGGTATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGACCAGTATGTGAAAGTGCCGGATACTCTC‑TACTTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCagcatcgtggttggtt       <  2:465881/251‑16 (MQ=255)

CACAAAACGCCGATCATCTTCCGCGCGACGCCGCAGTGGTTCGTCAGCATGGATCAGAAAGGTCTGCGTGCGCAGTCACTGAAAGAGATCAAAGGCGTGCAGTGGATCCCGGACTGGGGCCAGGCGCGTATCGAGTCGATGGTTGCTAACCGTCCTGACTGGTGTATCTCCCGTCAGCGCACCTGGGGTGTACCGATGTCACTGTTCGTGCACAAAGACACGGAAGAGCTGCATCCGCGTACCCTTGAACTGATGGAAGAAGTGGCAAAACGCGTTGAA‑GTCGATGGCATCCAGGCGTGGTGGGATCTCGATGCGAAAGAGATCCTCGGCGACGAAGCTGATCAGTACGTGAAAGTGCCGGACACATTG‑GATGTATGGTTTGACTCCGGATCTACCCACTCTTCTGTTGTTGACGTGCGTCCGGAATTTGCCGGTCACGCAGCGGACATGTATCTGGAAGGTTCTGACCAACACCGCGGCTGGTTCATGT  >  NC_000913/23615‑24104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`