BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,297	C→T	L303L (CTG→TTG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,297	0	C	T	98.4%	364.0 / NA	124	L303L (CTG→TTG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (1/0); major base T (59/61); minor base G (1/1); total (62/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 125 total aligned reads displayed.

CACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGT  >  NC_000913/23052‑23541

cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGttgctg                                                                                                                                                                                                                                                 >  2:410250/1‑251 (MQ=255)

     aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCtt                                                                                                                                                                                                                                            <  1:499036/251‑1 (MQ=255)

        ggaaaagttgttgTAAGCAACGTAAACGGCACAAGCTCGCTGGTGATTTGGGACACTCA‑GTCGTGGACTCTGCCTGCGAAACGCGCAATCTCTATTGTACCGGATTTCGACTATGCGCTGGTGCAGATCGACGGTAAGGCCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAAGGCAGCTTATCGGCGTGACCGATTACACCAGGCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGATGCGCtgtcc                                                                                                                                                                                                                                         <  2:305054/239‑5 (MQ=255)

                 gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑GACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGt                                                                                                                                                                                                                                 <  2:541161/250‑1 (MQ=255)

                        acAACGTTTACGGCGCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCGGCGAGCCGCGCAATCTCTAGTGCTCCTGATTTCGTCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTACAAGGTGCGGATCTTGAGTTGCTGCGCGTTACCCATCCGTTTATGGGc                                                                                                                                                                                                                         <  2:379661/250‑1 (MQ=255)

                            cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                                                                                                                                                                                                                     <  2:467737/251‑1 (MQ=255)

                             gttataggCCCAATCTCGTTGGTGATCTTGACCAC‑CTCGCCCTGGACTCTGCTTGCTAACCGCACAATCTCTATCGTACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTTGTTGAAAGTGTATTGCAGCGTATCGGCGCGACCGATTACACCATTCTCAGCACGGTAAAAGTTTCGTAGCTTGAGTTGCTGCGCTTTCCCCATCCGTTTATGGGCTTcta                                                                                                                                                                                                                    <  1:602154/245‑3 (MQ=255)

                             gTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGa                                                                                                                                                                                                                    <  1:602138/251‑1 (MQ=255)

                                   ggCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTcc                                                                                                                                                                                                              <  1:361159/251‑1 (MQ=255)

                                    gCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGATGTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                                                                                                                                                             >  2:616991/1‑251 (MQ=255)

                                         aTCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTTAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAAt                                                                                                                                                                                                        >  1:163618/1‑251 (MQ=255)

                                                 ggtgaTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGc                                                                                                                                                                                                 <  2:455731/246‑1 (MQ=255)

                                                   ggaTCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAt                                                                                                                                                                                              <  2:25533/249‑1 (MQ=255)

                                                     aTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATc                                                                                                                                                                                             >  1:277174/1‑250 (MQ=255)

                                                         ggACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt                                                                                                                                                                                        <  1:342290/251‑1 (MQ=255)

                                                          gACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt                                                                                                                                                                                        >  2:354655/1‑250 (MQ=255)

                                                              cactacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCACTCTCAGCACGGTAACAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCCATACTCGGCGATCACGTTAccc                                                                                                                                                                                    >  1:533463/5‑251 (MQ=255)

                                                                 tacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTACGCACGGTAAAAGGTGCGGCGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTgg                                                                                                                                                                                 >  2:258079/2‑251 (MQ=255)

                                                                     ccGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGc                                                                                                                                                                             >  1:400696/1‑251 (MQ=255)

                                                                      cGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGcc                                                                                                                                                                            <  1:297040/251‑1 (MQ=255)

                                                                         ggACTCTGCCTGCGTACCGCGCATGCTCTATTGCACCGGATTCCGATTATTCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGTTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGTCGATCACGTTACCCTGGATGCCGGt                                                                                                                                                                         <  1:205552/251‑1 (MQ=255)

                                                                                gcctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGt                                                                                                                                                                   <  1:409786/250‑1 (MQ=255)

                                                                                gcctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTGACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTg                                                                                                                                                                  <  2:435012/251‑1 (MQ=255)

                                                                                 cctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGGTGCCGGTACCGGTGc                                                                                                                                                                 >  1:249390/1‑251 (MQ=255)

                                                                                     cgaaCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGATGCCGGGACCGGTGCCGtt                                                                                                                                                             >  1:81179/3‑251 (MQ=255)

                                                                                        aCCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGCAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTccc                                                                                                                                                          >  2:412021/1‑249 (MQ=255)

                                                                                                aTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGcc                                                                                                                                                  >  2:264150/1‑251 (MQ=255)

                                                                                                    ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt                                                                                                                                                 <  1:462002/248‑1 (MQ=255)

                                                                                                    ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGGTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt                                                                                                                                                 <  2:190081/248‑1 (MQ=255)

                                                                                                    ctATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt                                                                                                                                                 <  1:185369/248‑1 (MQ=255)

                                                                                                    atATTGCGCCTGATGTCGACTATGGGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACTCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCGGCGCTTTGCCCATCCGTTTAGGGGCGTCGACGTTCCGGCAATCCCCGGCGATCACGTTACCCTGTATGCCGGTTCCCGTGCCGGTCACACCGCGCCt                                                                                                                                                 <  2:307181/247‑1 (MQ=255)

                                                                                                        tGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCCGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCCTCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTTGCCACg                                                                                                                                          >  1:89573/1‑251 (MQ=255)

                                                                                                        tGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCAc                                                                                                                                           <  1:312986/250‑1 (MQ=255)

                                                                                                          cacctgctttCGACTATGCGCTGGTGCAGATCGCCGGGCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGGGTAATGCAGCGTATCGGCGTGACCGATTAAACCCTTATCAGGACGGTAAACGGTGCGGAGCTTGAGTTGATGCGCTTTACCCATCCGTTTATGGGCTTCGCCGTTCCGGCGATCGTCGGCGATCACGGTACCATGGATGACGGTACCGGGGCCGTTCACACCGGGCCCGGccca                                                                                                                                           >  2:261303/8‑246 (MQ=255)

                                                                                                          cACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGc                                                                                                                                        >  1:393944/1‑251 (MQ=255)

                                                                                                            cctgagttCGACTAGGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCGGCGCTTGACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTGCACACCGCGCCTGGCCACGGccc                                                                                                                                      <  2:560331/245‑1 (MQ=255)

                                                                                                            cctgATTTCGACTAGGCGATGGTGCAGATCGACGGTCAGGCCGCGATTCTGGCGAAAGAGCTGTTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTGATGGGCTTCGACGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCGGGCCACGGccc                                                                                                                                      <  2:277174/248‑1 (MQ=255)

                                                                                                             ctgATTTCGACTATGCGCTGGAGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCCGAACGGTCAAAGGTGCGGAGCTTGCTTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGACGTTACCACAGCGCCTGGCCaaggaccg                                                                                                                                     >  2:371357/3‑244 (MQ=255)

                                                                                                                aTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgac                                                                                                                                  <  1:449906/251‑1 (MQ=255)

                                                                                                                 tttCGACTATGCGCTGGAGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACACATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACCCCGCGCCCGGCCACGGCCCGgacg                                                                                                                                 >  2:560343/1‑251 (MQ=255)

                                                                                                                 tatCGACTATGAGATGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACACCATTCTCAGCAAGGTAAAAGGTGGGGAGCTTTAGTTGCTGCGCTTTACCCATCCGTTTATGGGCATCGGAGGTCCGGCAAACCTCGGCGCTCACGTTACCCTGGCAGCCGGTACCGGTGCCGGTCACACCGCGACTGGCCACTGccacggcg                                                                                                                                 >  2:380478/3‑245 (MQ=255)

                                                                                                                  ttCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacg                                                                                                                                 >  1:173354/1‑250 (MQ=255)

                                                                                                                  tgcGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGTTGCCGTTCTCACCGCGCCTGGCCACGGCCCGgacg                                                                                                                                 <  1:404233/248‑1 (MQ=255)

                                                                                                                    cGACTATTCGCGGGTGAAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGTTTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGTTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCTTTACCCCGGATGCCGGTACCGGTGCCGTTCTCACCGTGccatgcctcgtcctggacgac                                                                                                                               <  2:393547/250‑20 (MQ=255)

                                                                                                                      aCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTATACCATTCTCAGCACGGTAAAAGGTGCGGAGCTGGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCGTCGTCGGTCCGTCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt                                                                                                                            <  1:584350/251‑1 (MQ=255)

                                                                                                                      aCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt                                                                                                                            >  1:341917/1‑251 (MQ=255)

                                                                                                                            cgcTGGTGCAGATCGACGGTCAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGAGGAGCTTGAGTTGCGGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGGTGCCGGTAACGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTCTGTGATc                                                                                                                      >  2:454645/1‑251 (MQ=255)

                                                                                                                            cgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATc                                                                                                                      <  1:7960/251‑1 (MQ=255)

                                                                                                                             gcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCg                                                                                                                     >  1:194646/1‑251 (MQ=255)

                                                                                                                                ggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAATGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTGATCGGTc                                                                                                                  >  1:432647/1‑251 (MQ=255)

                                                                                                                                   gCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAg                                                                                                                <  2:134832/250‑1 (MQ=255)

                                                                                                                                    cAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCGTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCCTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCTTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGTCGACTATGTGATCGTTCAGaa                                                                                                              <  2:274240/251‑1 (MQ=255)

                                                                                                                                     aGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGaaa                                                                                                             <  2:118171/251‑1 (MQ=255)

                                                                                                                                         cGACGGTCGGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAAGGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACTGCCCGGACGACTATGTGATCGGTCAGAAATACg                                                                                                         <  2:573239/251‑1 (MQ=255)

                                                                                                                                          gACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACgg                                                                                                        >  1:284640/1‑251 (MQ=255)

                                                                                                                                             ggTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGccc                                                                                                     >  1:490292/1‑250 (MQ=255)

                                                                                                                                              gTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTg                                                                                                    <  1:616991/251‑1 (MQ=255)

                                                                                                                                               tCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACGCCGCGCCTGGCCAc                                                                                                                                           <  1:473339/211‑1 (MQ=255)

                                                                                                                                                cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGCCCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                                                  <  1:376468/251‑1 (MQ=255)

                                                                                                                                                  ggccgtgctcgcggCGACAGATCTGGTTGAAAGCGTAATGCAGCGTATCGCCGTGACAGCTTAAACCATTCTCCGAACCGTAAAAGGTGCGGAGCTTCAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCCAGGCCCGGCCGCCTCTGTGACACGTCAGCAATACCGCCTggca                                                                                                 >  2:84054/13‑248 (MQ=255)

                                                                                                                                                  ggCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAGTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa                                                                                                <  1:54941/251‑1 (MQ=255)

                                                                                                                                                        gATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCCCGGCCCGGACGGCTATGTGATCGGGCAGAAATACGGCCTGGAAACCGcc                                                                                           >  1:4675/1‑249 (MQ=255)

                                                                                                                                                           tCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCAAGGTCAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTCCCCATCCGTTTATGGGCTTCGTCGTTCCGGCAATCCTCGGCGATCAAGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAAcc                                                                                       >  1:298719/1‑251 (MQ=255)

                                                                                                                                                            cTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGGACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGCTTATGGGCTTCGACGTTCCGGCACTCCTCGGCGATCACGTTACCCTGGATGCCGGGACCGGTGACGTTCACACCGCGCCTGGCCACGGCCCGGCCGACCCTGTGATACGACAGCAATACGGACTGGAAACCGCTCAccc                                                                                      >  2:558963/1‑251 (MQ=255)

                                                                                                                                                              ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTT‑ATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCgg                                                                                    <  1:140942/250‑1 (MQ=255)

                                                                                                                                                               gCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                                                                                   >  1:117879/1‑251 (MQ=255)

                                                                                                                                                                cGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGtt                                                                                  <  2:217460/251‑1 (MQ=255)

                                                                                                                                                                  aaaGATCTGGTTGAAAGCGAAATGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTAAAAGGAGAGGCGCTTGAGCTGCAGCGCTTTACCCCTCCGGTTATGAGCCTCGACGTTCCGGCAATCCTCGGCGATCACGTTACACTGGATGCCGGTACCGGTGACGTTCCCACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATCCCGCCTGGACACCGCAAACCCGGTTgg                                                                                >  2:360696/1‑251 (MQ=255)

                                                                                                                                                                    aGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCAATCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACCATGTGATCGGTCAGAAATACGGCCTGGAAACCGCCAACCCGGTTGGcc                                                                              >  2:577900/1‑251 (MQ=255)

                                                                                                                                                                          ggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGccc                                                                             <  1:534495/246‑1 (MQ=255)

                                                                                                                                                                          ggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTGCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACg                                                                        <  2:472645/251‑1 (MQ=255)

                                                                                                                                                                             tGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGc                                                                      <  1:90286/250‑1 (MQ=255)

                                                                                                                                                                              gAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGATTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCa                                                                     <  2:86887/250‑1 (MQ=255)

                                                                                                                                                                                aaGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACtt                                                                  <  1:439812/251‑1 (MQ=255)

                                                                                                                                                                                  gCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTAt                                                                >  2:132398/1‑251 (MQ=255)

                                                                                                                                                                                    gTAATGCAGCTTTTCGGCATGACCGATTACACCATGCTCAGCACGGTAAAAGGTGCGGCGCTGGATTGGCTGCGCGTTACCCGTCCGTTTATTGGCGTCGGCGTTCCGGCAATCCTCTGCGACCACGTTACCCTGGATGCCGGGACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGTACGACTATGCGATCGGTCAGAAATACGGGCTGAAAACCGCTAACCCGTTTGGCCCGGACGGTACTTATCt                                                              <  2:539674/251‑1 (MQ=255)

                                                                                                                                                                                      aaTGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTg                                                             <  1:248470/250‑1 (MQ=255)

                                                                                                                                                                                          cAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACGATGTGATCGGTCAGAAATACGGCCTGGAAACCGCAAACCCGGTTGGCCCGGACGGCACTTATCTGcccgg                                                        >  2:617390/1‑248 (MQ=255)

                                                                                                                                                                                             cGTATCGGCGTGACCGCTTACACCACTCCCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGCTCACACCGCGCCTGGCCACGGCCCGGGCGACTCTGTGATCGGTCCGAAATACGGGCTGGGAACCGCTAACCCGGTTGGCCCGGGCGGCACTTATCTGCCGGGCAc                                                     >  2:123293/1‑251 (MQ=255)

                                                                                                                                                                                               tATCGGCGTCACCGATTACACCATTCTCACCACGGTAAAAGGTGCGGAGATTGAGTTGCTGCGCTTTACCCCTCCGTTTATGGGCCTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCCCGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGGAACCGCTAAACCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACtt                                                   >  2:570144/1‑251 (MQ=255)

                                                                                                                                                                                                   ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                                               >  1:626910/1‑251 (MQ=255)

                                                                                                                                                                                                   ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACAGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                                               >  1:323120/1‑251 (MQ=255)

                                                                                                                                                                                                   ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAAGCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                                               <  2:166474/251‑1 (MQ=255)

                                                                                                                                                                                                      gTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAc                                            <  1:386103/251‑1 (MQ=255)

                                                                                                                                                                                                      gTGACCGATTAAACCATTCTCAGCACGGTACAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCACTCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGTAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCACTTATCTGCCGGGCACTTATCCGAc                                            >  1:484357/1‑251 (MQ=255)

                                                                                                                                                                                                          ccGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCCCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTg                                        <  2:405392/251‑1 (MQ=255)

                                                                                                                                                                                                              ttACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCCCTTATCTGCCGGGCACTTATCCGACGCTGGATg                                    >  2:385794/1‑251 (MQ=255)

                                                                                                                                                                                                                 cacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                 <  1:560343/251‑1 (MQ=255)

                                                                                                                                                                                                                 cacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCGTTCCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTTCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGTACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg                                 <  1:52594/251‑1 (MQ=255)

                                                                                                                                                                                                                  acCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGt                                >  1:212852/1‑251 (MQ=255)

                                                                                                                                                                                                                  acCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGt                                >  2:508579/1‑251 (MQ=255)

                                                                                                                                                                                                                       tctcAGCACGGTTAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCGTCGACGTTCCGGCAAGCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTGGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAc                            <  2:554175/250‑1 (MQ=255)

                                                                                                                                                                                                                           agCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                                            <  2:568845/197‑1 (MQ=255)

                                                                                                                                                                                                                           agCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCg                                                                            >  1:568845/2‑198 (MQ=255)

                                                                                                                                                                                                                              aCGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCa                     >  1:477007/1‑250 (MQ=255)

                                                                                                                                                                                                                                     aaGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAAc             <  1:410250/251‑1 (MQ=255)

                                                                                                                                                                                                                                         tGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGAcc         >  2:185427/1‑250 (MQ=255)

                                                                                                                                                                                                                                           cGGAGCTTGCGTTGCTACGCTTTCCCCCACCGTTTATGGGCTTAGACGTTCCGGCAATCCTCGGCGGTCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGAGCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtc       <  2:503557/251‑1 (MQ=255)

                                                                                                                                                                                                                                           cGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACAtc       <  1:441602/251‑1 (MQ=255)

                                                                                                                                                                                                                                                cTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGCAATACGGCCTGCAAACCGCTCACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGCACGTCTTCAACGCGACCGACAtcgtcgg  >  2:438064/1‑250 (MQ=255)

CACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAACGTCTTCAAAGCGAACGACATCGTCGT  >  NC_000913/23052‑23541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`