Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 23,270 G→A G294S (GGC→AGC)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091323,2700GA95.9% 362.3 / NA 122G294S (GGC→AGC) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (56/61);  minor base C (4/1);  total (60/62)
Fisher's exact test for biased strand distribution p-value = 2.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01

Only 100 of 122 total aligned reads displayed.

TTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAC  >  NC_000913/23025‑23515
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
ttGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGAACAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACAGATTACACCATTCTCAGCACg                                                                                                                                                                                                                                                   >  1:275607/1‑251 (MQ=255)
 tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGAGACCGATTACACAATTCTCAGCACgg                                                                                                                                                                                                                                                  >  1:217517/1‑251 (MQ=255)
       tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCCTTCTCAGCACGGTaaa                                                                                                                                                                                                                                              >  1:467094/2‑249 (MQ=255)
        cAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAgg                                                                                                                                                                                                                                           <  2:467094/251‑1 (MQ=255)
           gcggTCGATCAGGATGCACTGAAAGCAAAATTGGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAATATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGc                                                                                                                                                                                                                                        <  2:100799/248‑1 (MQ=255)
              gTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATGGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCTTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGa                                                                                                                                                                                                                                     <  1:332217/251‑1 (MQ=255)
                      ggATGCACTGAAAGCAAAAGTTGCCGGAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATGCTCAGCACGGTAAAAGGTGCGGAGCTTGAgt                                                                                                                                                                                                                             <  2:229941/251‑2 (MQ=255)
                           cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGttgctg                                                                                                                                                                                                                        >  2:410250/1‑251 (MQ=255)
                            ccTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCATCACGGTAAAAGGTGCGGAGCTTGAGTtgctgc                                                                                                                                                                                                                       <  1:470229/250‑1 (MQ=255)
                                aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCtt                                                                                                                                                                                                                   <  1:499036/251‑1 (MQ=255)
                                   ggaaaagttgttgTAAGCAACGTAAACGGCACAAGCTCGCTGGTGATTTGGGACACTCA‑GTCGTGGACTCTGCCTGCGAAACGCGCAATCTCTATTGTACCGGATTTCGACTATGCGCTGGTGCAGATCGACGGTAAGGCCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAAGGCAGCTTATCGGCGTGACCGATTACACCAGGCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGATGCGCtgtcc                                                                                                                                                                                                                <  2:305054/239‑5 (MQ=255)
                                            gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGGCCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGTTGCGCTTT‑ACCCATCCGtt                                                                                                                                                                                                      >  2:406545/1‑251 (MQ=255)
                                            gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑GACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGt                                                                                                                                                                                                       <  2:541161/250‑1 (MQ=255)
                                                 aaGCAACGTTAACAGCCCAATCTCGCTTGTTATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGCGTAATGCTGCTTATCTGCGTGACCGATTACACCATGCTCAGCTCGGTAAAAGGTGCGGATCTTGTGTTGCTGCGCTTT‑ACCCATCCGTTTATgg                                                                                                                                                                                                 <  2:564774/251‑1 (MQ=255)
                                                       cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCg                                                                                                                                                                                           <  2:467737/251‑1 (MQ=255)
                                                        gTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGa                                                                                                                                                                                          <  1:602138/251‑1 (MQ=255)
                                                              ggCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTcc                                                                                                                                                                                    <  1:361159/251‑1 (MQ=255)
                                                               gCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGATGTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                                                                                                                                   >  2:616991/1‑251 (MQ=255)
                                                                    aTCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTTAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAAt                                                                                                                                                                              >  1:163618/1‑251 (MQ=255)
                                                                        cGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                                                                                                                                          >  1:441006/1‑251 (MQ=255)
                                                                            ggtgaTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGc                                                                                                                                                                       <  2:455731/246‑1 (MQ=255)
                                                                              ggaTCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAt                                                                                                                                                                    <  2:25533/249‑1 (MQ=255)
                                                                                aTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATc                                                                                                                                                                   >  1:277174/1‑250 (MQ=255)
                                                                                    ggACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt                                                                                                                                                              <  1:342290/251‑1 (MQ=255)
                                                                                     gACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt                                                                                                                                                              >  2:354655/1‑250 (MQ=255)
                                                                                         cactacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCACTCTCAGCACGGTAACAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCCATACTCGGCGATCACGTTAccc                                                                                                                                                          >  1:533463/5‑251 (MQ=255)
                                                                                            tacGCCGTGGCCTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGAAGTTCCGGCAATCCTCGGCGATCACGTTACCCTgg                                                                                                                                                       >  1:405392/2‑251 (MQ=255)
                                                                                            tacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTACGCACGGTAAAAGGTGCGGCGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTgg                                                                                                                                                       >  2:258079/2‑251 (MQ=255)
                                                                                                ccGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGc                                                                                                                                                   >  1:400696/1‑251 (MQ=255)
                                                                                                 cGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGcc                                                                                                                                                  <  1:297040/251‑1 (MQ=255)
                                                                                                    ggACTCTGCCTGCGTACCGCGCATGCTCTATTGCACCGGATTCCGATTATTCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGTTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGTCGATCACGTTACCCTGGATGCCGGt                                                                                                                                               <  1:205552/251‑1 (MQ=255)
                                                                                                         ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGAGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTTCGGCAATCCTCGGCGATGCCGTTACCCTGGATGCCGGtcacgg                                                                                                                                          >  2:270598/1‑246 (MQ=255)
                                                                                                           gcctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTG‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTg                                                                                                                                        <  2:435012/251‑1 (MQ=255)
                                                                                                            cctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGGTGCCGGTACCGGTGc                                                                                                                                       >  1:249390/1‑251 (MQ=255)
                                                                                                            cctgcgAACCGCGCAATATCTCTCGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATATGGTTGAAAGCGTTATGCAGCGTATCGGCGTGACCGATTAAACCATTCTAAGCACGGAAAAAGGAGCGGGGCTTGAGATGCTGAGCTTTTAACC‑TCCGTTTATGGGCGTCGACGGTCAGGCAATCCTCGGCGATCACGTTaacatggggacagggacaggtgc                                                                                                                                       >  2:380386/1‑229 (MQ=255)
                                                                                                                   aCCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGCAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTccc                                                                                                                                >  2:412021/1‑249 (MQ=255)
                                                                                                                        gcAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACcgc                                                                                                                           >  1:63639/1‑251 (MQ=255)
                                                                                                                           aTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGcc                                                                                                                        >  2:264150/1‑251 (MQ=255)
                                                                                                                               ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGGTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt                                                                                                                       <  2:190081/248‑1 (MQ=255)
                                                                                                                                   tGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCCGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCCTCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTTGCCACg                                                                                                                >  1:89573/1‑251 (MQ=255)
                                                                                                                                   tGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCAc                                                                                                                 <  1:312986/250‑1 (MQ=255)
                                                                                                                                     cACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGc                                                                                                              >  1:393944/1‑251 (MQ=255)
                                                                                                                                      acctgATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTCT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGc                                                                                                                                                                       <  2:335332/189‑1 (MQ=255)
                                                                                                                                       cctgagttCGACTAGGCGCTGGTGCAGAGCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCGGCGCTTG‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTGCACACCGCGCCTGGCCACGGccc                                                                                                            <  2:560331/245‑1 (MQ=255)
                                                                                                                                       cctgATTTCGACTAGGCGATGGTGCAGATCGACGGTCAGGCCGCGATTCTGGCGAAAGAGCTGTTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTGATGGGCTTCGACGTTCCGGCAATCCGCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCGGGCCACGGccc                                                                                                            <  2:277174/248‑1 (MQ=255)
                                                                                                                                        ctgATTTCGACTATGCGCTGGAGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAACGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCCGAACGGTCAAAGGTGCGGAGCTTGCTTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGACGTTACCACAGCGCCTGGCCaaggaccg                                                                                                           >  2:371357/3‑244 (MQ=255)
                                                                                                                                          gATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCGCACCGCGCCTGGCCACGGCCCg                                                                                                           <  1:175888/249‑1 (MQ=255)
                                                                                                                                           aTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgac                                                                                                        <  1:449906/251‑1 (MQ=255)
                                                                                                                                            tttCGACTATGCGCTGGAGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACACATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACCCCGCGCCCGGCCACGGCCCGgacg                                                                                                       >  2:560343/1‑251 (MQ=255)
                                                                                                                                            tatCGACTATGAGATGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAACGATTACACCATTCTCAGCAAGGTAAAAGGTGGGGAGCTTTAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCATCGGAGGTCCGGCAAACCTCGGCGCTCACGTTACCCTGGCAGCCGGTACCGGTGCCGGTCACACCGCGACTGGCCACTGccacggcg                                                                                                       >  2:380478/3‑245 (MQ=255)
                                                                                                                                             ttCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGgacg                                                                                                       >  1:173354/1‑250 (MQ=255)
                                                                                                                                             tgcGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGTTGCCGTTCTCACCGCGCCTGGCCACGGCCCGgacg                                                                                                       <  1:404233/248‑1 (MQ=255)
                                                                                                                                               cGACTATTCGCGGGTGAAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGTTTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGTTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCTTTACCCCGGATGCCGGTACCGGTGCCGTTCTCACCGTGccatgcctcgtcctggacgac                                                                                                     <  2:393547/250‑20 (MQ=255)
                                                                                                                                               cGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGCTCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCCATTACACCATTCTCAGCCCGGTAAAAGGGGCGGAGATTGGGTTGCTGCGCTTT‑ACCCATCCGTTTATGTGCTTCGACGTTCCGGCAATCCTCGGCGCTCACGTTACCCTGGATGCCGGTACCCGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACt                                                                                                    >  2:102623/1‑251 (MQ=255)
                                                                                                                                                 aCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTATACCATTCTCAGCACGGTAAAAGGTGCGGAGCTGGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCGTCGTCGGTCCGTCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt                                                                                                  <  1:584350/251‑1 (MQ=255)
                                                                                                                                                 aCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTAt                                                                                                  >  1:341917/1‑251 (MQ=255)
                                                                                                                                                       cgcTGGTGCAGATCGACGGTCAGGCCGTGTTTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGAGGAGCTTGAGTTGCGGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGGTGCCGGTAACGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTCTGTGATc                                                                                            >  2:454645/1‑251 (MQ=255)
                                                                                                                                                       cgcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTCCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATc                                                                                            <  1:7960/251‑1 (MQ=255)
                                                                                                                                                        gcTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCg                                                                                           >  1:194646/1‑251 (MQ=255)
                                                                                                                                                           ggTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAATGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGCCGACTATGTGATCGGTc                                                                                        >  1:432647/1‑251 (MQ=255)
                                                                                                                                                              gCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAg                                                                                      <  2:134832/250‑1 (MQ=255)
                                                                                                                                                              gCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTccg                                                                                      >  1:214848/1‑248 (MQ=255)
                                                                                                                                                               cAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAGCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCGTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCCTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCTTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGTCGACTATGTGATCGTTCAGaa                                                                                    <  2:274240/251‑1 (MQ=255)
                                                                                                                                                                aGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGaaa                                                                                   <  2:118171/251‑1 (MQ=255)
                                                                                                                                                                    cGACGGTCGGGCCGTGATTCTGGCGAAAGAGCTGGTTGAAAGCGTAAGGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACTGCCCGGACGACTATGTGATCGGTCAGAAATACg                                                                               <  2:573239/251‑1 (MQ=255)
                                                                                                                                                                     gACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACgg                                                                              >  1:284640/1‑251 (MQ=255)
                                                                                                                                                                         gTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTg                                                                          <  1:616991/251‑1 (MQ=255)
                                                                                                                                                                          tCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACGCCGCGCCTGGCCAc                                                                                                                 <  1:473339/211‑1 (MQ=255)
                                                                                                                                                                           cAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGCCCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGa                                                                        <  1:376468/251‑1 (MQ=255)
                                                                                                                                                                             ggccgtgctcgcggCGACAGATCTGGTTGAAAGCGTAATGCAGCGTATCGCCGTGACAGCTTAAACCATTCTCCGAACCGTAAAAGGTGCGGAGCTTCAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCCAGGCCCGGCCGCCTCTGTGACACGTCAGCAATACCGCCTggca                                                                       >  2:84054/13‑248 (MQ=255)
                                                                                                                                                                             ggCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAGTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGaaa                                                                      <  1:54941/251‑1 (MQ=255)
                                                                                                                                                                                   gATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGGGCCGTTCACACCGCGCCTGGCCCCGGCCCGGACGGCTATGTGATCGGGCAGAAATACGGCCTGGAAACCGcc                                                                 >  1:4675/1‑249 (MQ=255)
                                                                                                                                                                                      tCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCAAGGTCAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑CCCCATCCGTTTATGGGCTTCGTCGTTCCGGCAATCCTCGGCGATCAAGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGGCAGAAATACGGCCTGGAAACCGCTAAcc                                                             >  1:298719/1‑251 (MQ=255)
                                                                                                                                                                                      tCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAAcc                                                             >  1:596847/1‑251 (MQ=255)
                                                                                                                                                                                         ggCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTT‑ATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCgg                                                          <  1:140942/250‑1 (MQ=255)
                                                                                                                                                                                          gCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGt                                                         >  1:117879/1‑251 (MQ=255)
                                                                                                                                                                                             aaaGATCTGGTTGAAAGCGAAATGCAGCGTATCGGCGTGACCGATTAAACCATTCTCAGCACGGTAAAAGGAGAGGCGCTTGAGCTGCAGCGCTTT‑ACCCCTCCGGTTATGAGCCTCGACGTTCCGGCAATCCTCGGCGATCACGTTACACTGGATGCCGGTACCGGTGACGTTCCCACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATCCCGCCTGGACACCGCAAACCCGGTTgg                                                      >  2:360696/1‑251 (MQ=255)
                                                                                                                                                                                                     ggTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTGCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACg                                              <  2:472645/251‑1 (MQ=255)
                                                                                                                                                                                                        tGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGc                                            <  1:90286/250‑1 (MQ=255)
                                                                                                                                                                                                         gAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGATTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCa                                           <  2:86887/250‑1 (MQ=255)
                                                                                                                                                                                                           aaGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTTCCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACtt                                        <  1:439812/251‑1 (MQ=255)
                                                                                                                                                                                                            aGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGTTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTa                                       <  2:154280/251‑1 (MQ=255)
                                                                                                                                                                                                             gCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTAt                                      >  2:132398/1‑251 (MQ=255)
                                                                                                                                                                                                               gTAATGCAGCTTTTCGGCATGACCGATTACACCATGCTCAGCACGGTAAAAGGTGCGGCGCTGGATTGGCTGCGCGTT‑ACCCGTCCGTTTATTGGCGTCGGCGTTCCGGCAATCCTCTGCGACCACGTTACCCTGGATGCCGGGACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGTACGACTATGCGATCGGTCAGAAATACGGGCTGAAAACCGCTAACCCGTTTGGCCCGGACGGTACTTATCt                                    <  2:539674/251‑1 (MQ=255)
                                                                                                                                                                                                                     cAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACGATGTGATCGGTCAGAAATACGGCCTGGAAACCGCAAACCCGGTTGGCCCGGACGGCACTTATCTGcccgg                              >  2:617390/1‑248 (MQ=255)
                                                                                                                                                                                                                          tATCGGCGTCACCGATTACACCATTCTCACCACGGTAAAAGGTGCGGAGATTGAGTTGCTGCGCTTT‑ACCCCTCCGTTTATGGGCCTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCCCGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGGAACCGCTAAACCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACtt                         >  2:570144/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                     >  1:626910/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACAGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                     >  1:323120/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAAGCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATcc                     <  2:166474/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGAc                  <  1:386103/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gTGACCGATTAAACCATTCTCAGCACGGTACAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCACTCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGTAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCACTTATCTGCCGGGCACTTATCCGAc                  >  1:484357/1‑251 (MQ=255)
                                                                                                                                                                                                                                   gACCGATTACCCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGc                >  1:496106/1‑251 (MQ=255)
                                                                                                                                                                                                                                     ccGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCCCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTg              <  2:405392/251‑1 (MQ=255)
                                                                                                                                                                                                                                        aTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTACGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGCTGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGCAACCGCTAACCCGGTTGGCCCGGACGGCAATTATCTGCCGGGCCCTTATCCGACGCTGGAt           >  2:235833/1‑251 (MQ=255)
                                                                                                                                                                                                                                         ttACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGCCGGCCCTTATCTGCCGGGCACTTATCCGACGCTGGATg          >  2:385794/1‑251 (MQ=255)
                                                                                                                                                                                                                                            cacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg       <  1:560343/251‑1 (MQ=255)
                                                                                                                                                                                                                                            cacCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCGTT‑CCCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTTCCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGTACGACTATGTGATCGGTCAGAAAGACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCg       <  1:52594/251‑1 (MQ=255)
                                                                                                                                                                                                                                             acCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGt      >  1:212852/1‑251 (MQ=255)
                                                                                                                                                                                                                                             acCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGt      >  2:508579/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  tctcAGCACGGTTAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCGTCGACGTTCCGGCAAGCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCGGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTGGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAc  <  2:554175/250‑1 (MQ=255)
                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      
TTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGCCCGGACGACTATGTGATCGGTCAGAAATACGGCCTGGAAACCGCTAACCCGGTTGGCCCGGACGGCACTTATCTGCCGGGCACTTATCCGACGCTGGATGGCGTGAAC  >  NC_000913/23025‑23515

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑