Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 23,161 A→T P257P (CCA→CCT)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091323,1610AT98.2% 355.7 / NA 115P257P (CCA→CCT) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (2/0);  major base T (62/48);  minor base G (0/2);  total (64/51)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGC  >  NC_000913/22914‑23407
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       
aCGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAt                                                                                                                                                                                                                                                      >  1:554175/1‑251 (MQ=255)
 cGGTCACCTGCACAAAGGCGCGAAGCCGGAACACTGGTGCGTTGACTGCAGTTCTGCGCTGGCGGCAGCGGAAGTTGCGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGCCGATCCGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAtt                                                                                                                                                                                                                                                     >  1:572363/1‑251 (MQ=255)
  gggcgccTGCTCAAAGGCGTGAAGCCGGTACACTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTGATCTGGACCAC‑TACGCCGTGGTCTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGAttt                                                                                                                                                                                                                                                    <  2:615824/246‑1 (MQ=255)
   gTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTc                                                                                                                                                                                                                                                   >  2:156490/1‑251 (MQ=255)
      aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGCGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCgcc                                                                                                                                                                                                                                                >  1:307181/1‑249 (MQ=255)
        cggcccaacggCGCGAAGCCGGTGCTCTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTGCTCCGTCCATCGACGTTGCTTGTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTGATCTGTACCAC‑TACGCCTTGGACTCTGCCTGCGAACCGCGCACTCTCTATTGCACCTGATGTCGACTa                                                                                                                                                                                                                                              <  2:82847/242‑1 (MQ=255)
            acaAAGGCGTGAGGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCCTCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCCCTATTGACCCAGATTTCGCCTATgcg                                                                                                                                                                                                                                          >  2:378150/1‑251 (MQ=255)
             caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgc                                                                                                                                                                                                                                         >  2:549816/1‑251 (MQ=255)
              aaaGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCGGCGAACCGCGGAATCTCTAGTGCTCTTGATTTCGACTATGCGCt                                                                                                                                                                                                                                        <  2:399481/251‑1 (MQ=255)
              aaaGGCGCGAAGCCGGTACACTGATGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCAATCGACGTTGCTTTTCAGGGGGTCGATCGGGCTGCATGGAAAGCAAAATTTACCGTAAGCACCGTTAACGGCCCAACCTCGATGGGGATCCGGACCCC‑CACGAATGGGACTTTGCCTCCGAACCGCACAATCTCTATTGCACTTGATTTCGACTATgcact                                                                                                                                                                                                                                        >  1:140196/1‑248 (MQ=255)
                aGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTTAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTgg                                                                                                                                                                                                                                      <  2:375086/251‑1 (MQ=255)
                       aagccggtacccTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt                                                                                                                                                                                                                               <  2:504190/240‑1 (MQ=255)
                           cgttacaatGGTGCGTTGACTGCCGTTCTGTGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTGAACGGCCCAATTTCGCTGGTGATCTGGCCCAC‑TACGCCGTGGCCTCTGCCTGCGACCCGCGCACTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGAc                                                                                                                                                                                                                           <  1:515373/243‑1 (MQ=255)
                            ggtacacggggttgttgacgtccGTGCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCATGCGGTCGATCAGGATGCGCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTTGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGTAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGTGCAGATCGACg                                                                                                                                                                                                                          <  2:80787/230‑1 (MQ=255)
                                cACTGGTGCGGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGAGCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCa                                                                                                                                                                                                                      <  2:14345/251‑1 (MQ=255)
                                  cTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAgg                                                                                                                                                                                                                    >  1:25533/1‑251 (MQ=255)
                                       gCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                                                                                                                                                                <  1:153609/250‑1 (MQ=255)
                                         gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                                                                                                                                                             >  1:541161/1‑251 (MQ=255)
                                         gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                                                                                                                                                             >  1:367818/1‑251 (MQ=255)
                                          ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAtt                                                                                                                                                                                                            >  2:439812/1‑251 (MQ=255)
                                             aCTGCCGTTCAGAGCTCGCGGAAGCGGAAGGTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTACTTTTCAGGCGGTCGCACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTCACGGCCCAATATCGCTGGTGATCTGGAAAAC‑TCCGCCGTGGACTCTGCCTGAGAACCGCGCAATCTCTCTTTCACCTGATTTCGACTATGCGCTGGTGCAGATCGCGGGTTAGGCCGTGATTCTg                                                                                                                                                                                                         >  2:584350/1‑251 (MQ=255)
                                                 cctttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGa                                                                                                                                                                                                     <  2:124441/248‑1 (MQ=255)
                                                  cGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGa                                                                                                                                                                                                     >  2:449906/1‑250 (MQ=255)
                                                    ttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGaa                                                                                                                                                                                                    >  1:486821/1‑249 (MQ=255)
                                                     tCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGTGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAg                                                                                                                                                                                                  <  1:417220/250‑1 (MQ=255)
                                                        gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCt                                                                                                                                                                                              >  1:118171/1‑251 (MQ=255)
                                                           cTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGt                                                                                                                                                                                           >  1:500632/1‑251 (MQ=255)
                                                             ggcggcAGCGGAAGTTGAGTATTACGACAAACCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTg                                                                                                                                                                                         >  2:361159/1‑251 (MQ=255)
                                                               cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                                                                                                                                                        >  1:572832/1‑250 (MQ=255)
                                                               cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGa                                                                                                                                                                                        >  2:469775/1‑250 (MQ=255)
                                                                 gaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCGCGCTGGTGATCTGGACCAC‑TACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAg                                                                                                                                                                                     <  2:594982/251‑1 (MQ=255)
                                                                   agcggaagTTGAGAATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGAAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCAGGACCAC‑TACCACGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCACCTGATTTCGCATATGCGCTGGTGCTGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCg                                                                                                                                                                                   >  1:605616/1‑251 (MQ=255)
                                                                     cggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGt                                                                                                                                                                                  >  1:196694/1‑250 (MQ=255)
                                                                      ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                                                                                                                                                 >  1:455731/1‑250 (MQ=255)
                                                                               gTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCACGCCGTGTTTTTGGCGACCGATCAGGTTGAAAGCGTAATGCAGCgg                                                                                                                                                                        >  2:519533/1‑249 (MQ=255)
                                                                                tATTACGACAAAACTTCTCCGTCCATCGCCGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGACCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCCGGCCGCGATTCTTGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTa                                                                                                                                                                       >  2:175888/1‑250 (MQ=255)
                                                                                tATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAt                                                                                                                                                                      >  1:312301/1‑251 (MQ=255)
                                                                                    aCGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                                                                                                                                                  <  1:156490/251‑1 (MQ=255)
                                                                                    aCGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                                                                                                                                                  >  1:14834/1‑251 (MQ=255)
                                                                                        cAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                                                                                                                                                               >  1:552464/1‑250 (MQ=255)
                                                                                          aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGAGCAGATCGCCGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                                                                                                                                             >  1:8463/1‑250 (MQ=255)
                                                                                          aaaCTTCTCCGTCCATCGACGTTGCGTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                                                                                                                                                            <  2:8463/251‑1 (MQ=255)
                                                                                            aCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGa                                                                                                                                                          <  1:549816/251‑1 (MQ=255)
                                                                                               tctcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAtt                                                                                                                                                        <  1:481011/250‑1 (MQ=255)
                                                                                                 tcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTGGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCTGCGTCTCGGCGTGATCGATTac                                                                                                                                                      >  2:7960/1‑250 (MQ=255)
                                                                                                  ccGTCCATCGACGTTGCTTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGCGCAGCTCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTACTGAAGCGTATCGGCGTGGCCGGTTacac                                                                                                                                                    >  2:54941/1‑251 (MQ=255)
                                                                                                   cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                                                                                                                                                   >  1:86887/1‑251 (MQ=255)
                                                                                                        aTCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtct                                                                                                                                              >  1:134832/1‑251 (MQ=255)
                                                                                                          cGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca                                                                                                                                            <  1:469255/251‑2 (MQ=255)
                                                                                                           gACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcag                                                                                                                                           <  2:572832/251‑3 (MQ=255)
                                                                                                            aCGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc                                                                                                                                          >  1:279700/1‑248 (MQ=255)
                                                                                                             cGTTGCTTTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGCGAAAAATCTGGATGAAAGCGTAATGCAGCGTATCGGAGTGACAAATTACACCAttataacac                                                                                                                                         >  2:326224/1‑244 (MQ=255)
                                                                                                               ttGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGAACAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACAGATTACACCATTCTCAGCACg                                                                                                                                       >  1:275607/1‑251 (MQ=255)
                                                                                                                tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGAGACCGATTACACAATTCTCAGCACgg                                                                                                                                      >  1:217517/1‑251 (MQ=255)
                                                                                                                      tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCCTTCTCAGCACGGTaaa                                                                                                                                  >  1:467094/2‑249 (MQ=255)
                                                                                                                       cAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAgg                                                                                                                               <  2:467094/251‑1 (MQ=255)
                                                                                                                          gcggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTATATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTa                                                                                                                                                       <  1:565926/221‑1 (MQ=255)
                                                                                                                            ggTCGAGCAGGAAGCACTGAAACCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAGTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACTCCATTCTCAGCCCGGTAAAAGTTGCgg                                                                                                                          <  2:405879/250‑1 (MQ=255)
                                                                                                                             gTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATGGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCTTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGa                                                                                                                         <  1:332217/251‑1 (MQ=255)
                                                                                                                                     ggATGCACTGAAAGCAAAAGTTGCCGGAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATGCTCAGCACGGTAAAAGGTGCGGAGCTTGAgt                                                                                                                 <  2:229941/251‑2 (MQ=255)
                                                                                                                                          cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGttgctg                                                                                                            >  2:410250/1‑251 (MQ=255)
                                                                                                                                               aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCtt                                                                                                       <  1:499036/251‑1 (MQ=255)
                                                                                                                                                  ggaaaagttgttgTAAGCAACGTAAACGGCACAAGCTCGCTGGTGATTTGGGACACTCA‑GTCGTGGACTCTGCCTGCGAAACGCGCAATCTCTATTGTACCGGATTTCGACTATGCGCTGGTGCAGATCGACGGTAAGGCCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAAGGCAGCTTATCGGCGTGACCGATTACACCAGGCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGATGCGCtgtcc                                                                                                    <  2:305054/239‑5 (MQ=255)
                                                                                                                                                           gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGGCCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGTTGCGCTTT‑ACCCATCCGtt                                                                                          >  2:406545/1‑251 (MQ=255)
                                                                                                                                                           gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑GACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGt                                                                                           <  2:541161/250‑1 (MQ=255)
                                                                                                                                                                aaGCAACGTTAACAGCCCAATCTCGCTTGTTATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGCGTAATGCTGCTTATCTGCGTGACCGATTACACCATGCTCAGCTCGGTAAAAGGTGCGGATCTTGTGTTGCTGCGCTTT‑ACCCATCCGTTTATgg                                                                                     <  2:564774/251‑1 (MQ=255)
                                                                                                                                                                  acAACGTTTACGGCGCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCGGCGAGCCGCGCAATCTCTAGTGCTCCTGATTTCGTCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTACAAGGTGCGGATCTTGAGTTGCTGCGCGTT‑ACCCATCCGTTTATGGGc                                                                                   <  2:379661/250‑1 (MQ=255)
                                                                                                                                                                    aaCGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCtt                                                                                 >  1:377206/1‑251 (MQ=255)
                                                                                                                                                                      cGTTAACGGCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCg                                                                               <  2:467737/251‑1 (MQ=255)
                                                                                                                                                                       gttataggCCCAATCTCGTTGGTGATCTTGACCAC‑CTCGCCCTGGACTCTGCTTGCTAACCGCACAATCTCTATCGTACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTTGTTGAAAGTGTATTGCAGCGTATCGGCGCGACCGATTACACCATTCTCAGCACGGTAAAAGTTTCGTAGCTTGAGTTGCTGCGCTTT‑CCCCATCCGTTTATGGGCTTcta                                                                              <  1:602154/245‑3 (MQ=255)
                                                                                                                                                                             ggCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTcc                                                                        <  1:361159/251‑1 (MQ=255)
                                                                                                                                                                              gCCCAATCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGATGTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCg                                                                       >  2:616991/1‑251 (MQ=255)
                                                                                                                                                                                   aTCTCGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTTAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAAt                                                                  >  1:163618/1‑251 (MQ=255)
                                                                                                                                                                                       cGCTGGTGATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc                                                              >  1:441006/1‑251 (MQ=255)
                                                                                                                                                                                           ggtgaTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGc                                                           <  2:455731/246‑1 (MQ=255)
                                                                                                                                                                                             ggaTCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAt                                                        <  2:25533/249‑1 (MQ=255)
                                                                                                                                                                                                   ggACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt                                                  <  1:342290/251‑1 (MQ=255)
                                                                                                                                                                                                    gACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGGGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt                                                  >  2:354655/1‑250 (MQ=255)
                                                                                                                                                                                                        cactacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGGGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCACTCTCAGCACGGTAACAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCCATACTCGGCGATCACGTTAccc                                              >  1:533463/5‑251 (MQ=255)
                                                                                                                                                                                                           tacGCCGTGGCCTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGGGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGAAGTTCCGGCAATCCTCGGCGATCACGTTACCCTgg                                           >  1:405392/2‑251 (MQ=255)
                                                                                                                                                                                                           tacGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTACGCACGGTAAAAGGTGCGGCGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTgg                                           >  2:258079/2‑251 (MQ=255)
                                                                                                                                                                                                               ccGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGc                                       >  1:400696/1‑251 (MQ=255)
                                                                                                                                                                                                                cGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGcc                                      <  1:297040/251‑1 (MQ=255)
                                                                                                                                                                                                                   ggACTCTGCCTGCGTACCGCGCATGCTCTATTGCACCGGATTCCGATTATTCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGTTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGTCGATCACGTTACCCTGGATGCCGGt                                   <  1:205552/251‑1 (MQ=255)
                                                                                                                                                                                                                        ctgcctgcGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGAGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTTCGGCAATCCTCGGCGATGCCGTTACCCTGGATGCCGGtcacgg                              >  2:270598/1‑246 (MQ=255)
                                                                                                                                                                                                                          gcctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGt                             <  1:409786/250‑1 (MQ=255)
                                                                                                                                                                                                                          gcctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTG‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTg                            <  2:435012/251‑1 (MQ=255)
                                                                                                                                                                                                                           cctgcgAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGGTGCCGGTACCGGTGc                           >  1:249390/1‑251 (MQ=255)
                                                                                                                                                                                                                           cctgcgAACCGCGCAATATCTCTCGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATATGGTTGAAAGCGTTATGCAGCGTATCGGCGTGACCGATTAAACCATTCTAAGCACGGAAAAAGGAGCGGGGCTTGAGATGCTGAGCTTTTAACC‑TCCGTTTATGGGCGTCGACGGTCAGGCAATCCTCGGCGATCACGTTaacatggggacagggacaggtgc                           >  2:380386/1‑229 (MQ=255)
                                                                                                                                                                                                                               cgaaCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCCCGTTACCCTGGATGCCGGGACCGGTGCCGtt                       >  1:81179/3‑251 (MQ=255)
                                                                                                                                                                                                                                  aCCGCGCAATCTCTATTGCACCTGATTTCGCCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGCAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTccc                    >  2:412021/1‑249 (MQ=255)
                                                                                                                                                                                                                                       gcAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACcgc               >  1:63639/1‑251 (MQ=255)
                                                                                                                                                                                                                                          aTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCCTCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGcc            >  2:264150/1‑251 (MQ=255)
                                                                                                                                                                                                                                              ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt           <  1:462002/248‑1 (MQ=255)
                                                                                                                                                                                                                                              ctATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGGTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt           <  2:190081/248‑1 (MQ=255)
                                                                                                                                                                                                                                              ctATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCt           <  1:185369/248‑1 (MQ=255)
                                                                                                                                                                                                                                              atATTGCGCCTGATGTCGACTATGGGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACTCCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCGGCGCTTT‑GCCCATCCGTTTAGGGGCGTCGACGTTCCGGCAATCCCCGGCGATCACGTTACCCTGTATGCCGGTTCCCGTGCCGGTCACACCGCGCCt           <  2:307181/247‑1 (MQ=255)
                                                                                                                                                                                                                                                  tGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCCGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGGAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCCTCCGGTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTTGCCACg    >  1:89573/1‑251 (MQ=255)
                                                                                                                                                                                                                                                  tGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCAc     <  1:312986/250‑1 (MQ=255)
                                                                                                                                                                                                                                                    cACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGc  >  1:393944/1‑251 (MQ=255)
                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                       
ACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTAATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTT‑ACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTTACCCTGGATGCCGGTACCGGTGCCGTTCACACCGCGCCTGGCCACGGC  >  NC_000913/22914‑23407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑