| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,108 | A→T | Q591L (CAG→CTG) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,108 | 0 | A | T | 98.5% | 209.1 / ‑6.0 | 66 | Q591L (CAG→CTG) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (30/35); minor base G (0/1); total (30/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
TGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGA > NC_000913/1862‑2354 | tGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTg > 1:309423/1‑251 (MQ=255)tGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTAATCCCATCTGTTg > 1:327438/1‑251 (MQ=255) gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTg < 1:191830/250‑1 (MQ=255) gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACGTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt < 1:370354/249‑1 (MQ=255) aaTGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAATCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCTCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATgc < 2:485518/251‑1 (MQ=255) aaTGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTGCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATgc < 1:306942/251‑1 (MQ=255) cctaaaTCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAt < 1:488778/247‑1 (MQ=255) ggtGGAAAACTGGCAGGAAGACCTGGCCCAGGCCAAAGAGCCGTTTAATGTCGGGCGCTTAATTCGCGTCGTGAAAGAATATCATCTGCTGGACCCGGTCATTGTTGACTGCACTTCCAGACAGGCAGTG‑GCGGAGCAATATGCCGACTTCGTGCGCGTAGGTTTCCACGTTGGCGCTCTGAGCAAAAAGGCCAACACCTCGTCGATGGATTACTACCAGCTGTTGCGTCATGCGGCGGAATAATCgcggt < 2:206870/249‑2 (MQ=255) cTGGCAGGAAGAACTGGCCCAAGCCAAAGAGCCGTTTAATATCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGAGGGCGAAAAATCGGGGCGTAAATTcc > 2:398032/1‑251 (MQ=255) gCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCctct > 1:385052/1‑251 (MQ=255) aGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCCTCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTAt > 2:107739/1‑251 (MQ=255) tGGCGCACGCCACAGAGCCGTTTCATCTCGGGCGCTTCATTCGCCTCGTGAAAGAATATCATCTGCTGCACCCGGTCATTGTTGACTGCCCTTCCAGCCAGGCAGTG‑GCGGATCAATCTGCCGACTTCTTGCCCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCCCGTCGCTGGATTACTACCATCTGTTGCTGAATTGGGCGGAAAAATCACGGCGTACATTACTCTATGCCAC‑Caa > 2:607404/1‑250 (MQ=255) ggCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACg < 2:128922/251‑1 (MQ=255) ggCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCGATGACAC‑CAAc < 2:79220/250‑1 (MQ=255) gcAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTg < 1:274815/251‑1 (MQ=255) cAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTgg < 1:76037/251‑1 (MQ=255) aaGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGa < 1:454678/251‑1 (MQ=255) tttAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTTGATTACCGGt > 2:374643/1‑251 (MQ=255) aaTTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaa > 1:471191/1‑250 (MQ=255) ccTCGTGGAAGAATATCATCTGCTGAACCCGGTCATTGTTGCCTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CCACGTTGGGGCTGGATTACCGGTTTTTGGGAACCTGCAAAATCTGc > 2:360149/1‑251 (MQ=255) cGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGGCGAATTCTTGCGCGAAGGTTTCCAAGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGCTTACTAACCTCTGTTGCGTCCTGCGGCGGGAAAATCGCGGCGTAAATTCCTCTCTGACAC‑CAACGTTGGGGCTGGGTTACCGGTTCTTGAAAACCTGCAAAATCTggtcc > 1:350956/1‑247 (MQ=255) aaTATCATCTGCTGAACCCGGTCATTGTTGCCTGCACTTCCAGCCCAGCAGTG‑GCGGATCTATATGTCGACTTCTAGCGCGAAGGGTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCCGAAAAATCGCGGTGTAAATTCATTCATGACAC‑CAAAGATGGGGCTGGATTAACGGGTATTGAGAACCTGCAAAATATGCTCAATGctggg > 2:105358/1‑247 (MQ=255) atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtg < 1:444457/251‑1 (MQ=255) tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGGAAATTCCTCTATGCCAC‑CAACGTTGGGGCTGGATTAACGGCTATTGAGGACCTGCAAAATATGCttcatgccggtgg > 2:267696/1‑239 (MQ=255) atcatcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgat < 1:57667/251‑1 (MQ=255) catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatga < 1:77990/251‑1 (MQ=255) atcTGCTGTACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCCGTG‑GCGGATCAATATGACGACTTCTTGCGCGAAGGTTTCCCCGTTGTCACCCCGAACAAAAAGGGCAAAACCTCGTCGATGGATTACTACCATCTGTTGCGGCAAGCAGCGGAAAAATTGCGGCGTAAAGTCATCTATGAAACACAA‑GATGGGGCTGGATTACCGGTTATTGAGAAACTGCAGCATCTGCTCAAtacgggggatgg > 2:545917/1‑239 (MQ=255) gctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTAGGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATtg < 1:314137/250‑1 (MQ=255) gAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGACCCTGCAAAATCTGCTCAATGCTGGTGATGAATtgatg < 1:100849/250‑1 (MQ=255) aCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAg < 1:473525/251‑1 (MQ=255) cccGGTCATTGTTGACTGCACTTCCAGCGAGGCA‑TGTGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAATGCCAACACCTCGTCGATGGATTACTACCGGCGGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGGTGGGGCTGGATTACCGGGGAGGGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGt < 1:228698/251‑1 (MQ=255) acggccATTGTTGACTGAACTTCCAGCCAGGCAGTG‑GCGGACCCATATGGCGACTTCTTGCGCGAAGGTTTCCACCTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCATCATGCGGCGGAAAACTCGCGGGGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGACAAATCTGCTCAATGCTGGTGATGACTTGCAGAAGtt > 2:528503/6‑251 (MQ=255) gTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACGTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCTGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAGCCTGCAAAATCGGCTCAATGCTGGTTATGAATTGATGGAGTtctc < 2:36198/251‑1 (MQ=255) cATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTcc < 1:572217/250‑1 (MQ=255) aTTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGTATTGATGAAGTTCTCCgg > 2:521732/1‑251 (MQ=255) ggtttaCTGCACTTCCATCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGTATTACCGGTGATTGAGAACCTGCAAAATCTGCTCAATGCTGGTTATTAATGGATGAAGTtcggcggt < 2:30299/245‑7 (MQ=255) ttgACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAtt > 2:160704/1‑251 (MQ=255) tgACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGGGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAAAGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGAAttc > 1:535943/1‑251 (MQ=255) aCTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCCCGGTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CCACGTTGGGGCTGGATTACCGGTTCTTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGAATTGATGAAGTTCTCCGGCAttctt > 2:129073/1‑251 (MQ=255) aCTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctt > 1:197974/1‑251 (MQ=255) tGCACTTCCAGCCAGGCAGTG‑GCGGATTAATATGCCGACTTCTTGCGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGGTTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGGTTACCGGTTATTGGGAACCTGCAAAATCTGCTCAATGATGGTGATGAATTGATGAAGTTCTCCGGCAttctttc > 2:390980/1‑251 (MQ=255) gCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttca < 1:284524/251‑2 (MQ=255) cTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCCACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAAAACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTACTCTATGACAC‑CAAAGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAACATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGt > 1:118569/1‑251 (MQ=255) ccagGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCTTCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCTTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGTGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:278152/251‑1 (MQ=255) cAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGGAGGGTTCCACGGTGTCCCGCCGAACAAAAAAGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGa > 2:61587/1‑142 (MQ=255) cAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGa < 1:61587/142‑1 (MQ=255) g‑gCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGACGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCt > 1:89774/1‑251 (MQ=255) g‑gCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCt < 1:374643/251‑1 (MQ=255) atGCCGACTTCTTGCGCGAATGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGTGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTTCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGtt < 1:251784/250‑1 (MQ=255) gcgAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGt < 1:521732/251‑1 (MQ=255) aGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTtct < 2:201273/251‑1 (MQ=255) ttCCACGTTGTCACGCCGAACAAAAAGGCCAATACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCATGTTAGACGAAGGCATGAGTTTCTCCgt > 2:112968/1‑250 (MQ=255) gTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGAc > 2:132251/1‑251 (MQ=255) tGTCACGCCGAACAAAACGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGAct > 2:83810/1‑249 (MQ=255) cACGCCGAACAAAAAGGCCAACCCCTCGTCGTTGGATTACTACCATCTGTTGCGTCATGCGGCGGACAAATCGCGGCGTAAATTCCTATATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCACAATCTGCTCAATGCTGGTCCTGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGActactc > 2:395204/1‑246 (MQ=255) cAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCggg < 1:79413/251‑1 (MQ=255) aaaaGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGaa < 2:306512/251‑1 (MQ=255) aaaGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGaaa < 1:553686/251‑1 (MQ=255) gCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCCGCGGAAACATCGCGGCGTAAATTCCTCCATGACAC‑CAAAGTTGCAGCTGGATTACCGGCTATTGAGAAACTGAAAAATCTGCTCAATGCTGGTGCTGACTTGCTGAAGTTCTCCGGCATTCTTTCAGGTTAGCTTTCTTATATCTTCGGCAAGTTCGACGAAGCCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATggg > 1:321319/1‑251 (MQ=255) cgATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAAcc < 1:66435/250‑1 (MQ=255) gATGGATTACTACCATCTGTTGCGTCAAGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑AAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCgg > 1:461446/1‑251 (MQ=255) aTGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGa < 1:390980/251‑1 (MQ=255) tactacCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCcgc < 1:107739/250‑1 (MQ=255) acCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgat < 2:471191/251‑1 (MQ=255) ccATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgatg > 1:393869/1‑251 (MQ=255) cATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACTACTCTGGCGCGGGAAATGGGTTATACCGAACCGGATCCGCGAgatga < 1:40464/251‑1 (MQ=255) | TGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTTAGACGAAGGCATGAGTTTCTCCGAGGCGACCACGCTGGCGCGGGAAATGGGTTATACCGAACCGGACCCGCGAGATGA > NC_000913/1862‑2354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |