Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 23,116 C→T T242T (ACC→ACT)  ileS → isoleucine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091323,1160CT97.2% 337.1 / NA 108T242T (ACC→ACT) ileSisoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (62/41);  total (63/45)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 108 total aligned reads displayed.

AAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTT  >  NC_000913/22875‑23359
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   
   ccAACATCATCCGAGCGCTGGGAAAAATCATCGGCAAAGGCCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGAG‑ATCTGGACCAC‑TACGCCGTGGAct                                                                                                                                                                                                                                         >  2:297040/1‑251 (MQ=255)
     aaCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGActct                                                                                                                                                                                                                                       >  1:488819/1‑251 (MQ=255)
      aCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTctg                                                                                                                                                                                                                                      >  2:469255/1‑251 (MQ=255)
       catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCCAAATTCGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCAC‑TACGCCGTGGACTctgc                                                                                                                                                                                                                                     >  2:248470/1‑251 (MQ=255)
       catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTctgc                                                                                                                                                                                                                                     >  1:166474/1‑251 (MQ=255)
                cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACACAGGCGCGAAGCCGGAACACTGGTGCGTTGCCTGCCGTTCTGCGCTGACGGAAGCTGAAGTTAAGGATTACGACAAACCTTCTCCGTCCATCGACGGTGCTTTTCAGGCGGTCAATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCTTTACCGGCCCAATCTCACTG‑TAGATCTTGACCAC‑TACGCCATgggttcacccttcagacc                                                                                                                                                                                                                            >  1:305054/1‑235 (MQ=255)
                cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAAcc                                                                                                                                                                                                                            >  2:140942/1‑251 (MQ=255)
                cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGATCGATCAGGATGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAAcc                                                                                                                                                                                                                            >  1:375086/1‑251 (MQ=255)
                        gCAAAATCATCGTCAACGGTCACCTGCACAAATGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCGGCCTGCGAACCGCGCAAtc                                                                                                                                                                                                                    <  2:415624/251‑1 (MQ=255)
                             atcatcGGCAACGGTCACCTGCACAAAGGCGCGCAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAACGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGCACCACGCAACCTCTAt                                                                                                                                                                                                               >  2:462002/1‑251 (MQ=255)
                                 tcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGATGGCGGAAGCGGAAGATGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAAACTATATTGc                                                                                                                                                                                                            >  1:564774/1‑250 (MQ=255)
                                  cGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAc                                                                                                                                                                                                          >  2:481011/1‑251 (MQ=255)
                                       aCGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAt                                                                                                                                                                                                     >  1:554175/1‑251 (MQ=255)
                                        cGGTCACCTGCACAAAGGCGCGAAGCCGGAACACTGGTGCGTTGACTGCAGTTCTGCGCTGGCGGCAGCGGAAGTTGCGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGCCGATCCGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAtt                                                                                                                                                                                                    >  1:572363/1‑251 (MQ=255)
                                         gggcgccTGCTCAAAGGCGTGAAGCCGGTACACTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGGACCAC‑TACGCCGTGGTCTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGAttt                                                                                                                                                                                                   <  2:615824/246‑1 (MQ=255)
                                          gTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTc                                                                                                                                                                                                  >  2:156490/1‑251 (MQ=255)
                                           tCACCTTCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACGGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCa                                                                                                                                                                                                           <  2:463459/241‑1 (MQ=255)
                                           tCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCa                                                                                                                                                                                                           >  1:463459/1‑241 (MQ=255)
                                             aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGCGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCgcc                                                                                                                                                                                               >  1:307181/1‑249 (MQ=255)
                                               cggcccaacggCGCGAAGCCGGTGCTCTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTGCTCCGTCCATCGACGTTGCTTGTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGTACCAC‑TACGCCTTGGACTCTGCCTGCGAACCGCGCACTCTCTATTGCACCTGATGTCGACTa                                                                                                                                                                                             <  2:82847/242‑1 (MQ=255)
                                                   acaAAGGCGTGAGGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCCTCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCCCTATTGACCCAGATTTCGCCTATgcg                                                                                                                                                                                         >  2:378150/1‑251 (MQ=255)
                                                     aaaGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCGGCGAACCGCGGAATCTCTAGTGCTCTTGATTTCGACTATGCGCt                                                                                                                                                                                       <  2:399481/251‑1 (MQ=255)
                                                     aaaGGCGCGAAGCCGGTACACTGATGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCAATCGACGTTGCTTTTCAGGGGGTCGATCGGGCTGCATGGAAAGCAAAATTTACCGTAAGCACCGTTAACGGCCCAACCTCGATGGGG‑ATCCGGACCCC‑CACGAATGGGACTTTGCCTCCGAACCGCACAATCTCTATTGCACTTGATTTCGACTATgcact                                                                                                                                                                                       >  1:140196/1‑248 (MQ=255)
                                                       aGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTTAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTgg                                                                                                                                                                                     <  2:375086/251‑1 (MQ=255)
                                                         gcgcGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTCCGACAAAACTTATCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGGTGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTg                                                                                                                                                                                   >  1:405879/1‑251 (MQ=255)
                                                              aagccggtacccTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt                                                                                                                                                                              <  2:504190/240‑1 (MQ=255)
                                                                 ccggtacACTGGTGCTGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCg                                                                                                                                                                            <  2:444735/244‑1 (MQ=255)
                                                                  cgttacaatGGTGCGTTGACTGCCGTTCTGTGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTGAACGGCCCAATTTCGCTGGTG‑ATCTGGCCCAC‑TACGCCGTGGCCTCTGCCTGCGACCCGCGCACTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGAc                                                                                                                                                                          <  1:515373/243‑1 (MQ=255)
                                                                   ggtacacggggttgttgacgtccGTGCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCATGCGGTCGATCAGGATGCGCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTTGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGTAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGTGCAGATCGACg                                                                                                                                                                         <  2:80787/230‑1 (MQ=255)
                                                                      ccACTGCTGCGTTGCCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCGCCGTCCATCGACGTTGCTTTTCAGGCGGTCGAGCAGGATGCTCGGAAAGCAAAATCTGCCGTAAGCAGCGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑GACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGTACCCGATTTCCGCTATGCGCTGGTGGTGATCGACGGTc                                                                                                                                                                      <  2:232793/250‑1 (MQ=255)
                                                                       cACTGGTGCGGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGAGCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCa                                                                                                                                                                     <  2:14345/251‑1 (MQ=255)
                                                                         cTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAgg                                                                                                                                                                   >  1:25533/1‑251 (MQ=255)
                                                                            gTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCg                                                                                                                                                                >  2:338578/1‑251 (MQ=255)
                                                                              gCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                                                                                                               <  1:153609/250‑1 (MQ=255)
                                                                                gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                                                                                                            >  1:367818/1‑251 (MQ=255)
                                                                                 ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAtt                                                                                                                                                           >  2:439812/1‑251 (MQ=255)
                                                                                    aCTGCCGTTCAGAGCTCGCGGAAGCGGAAGGTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTACTTTTCAGGCGGTCGCACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTCACGGCCCAATATCGCTGGTG‑ATCTGGAAAAC‑TCCGCCGTGGACTCTGCCTGAGAACCGCGCAATCTCTCTTTCACCTGATTTCGACTATGCGCTGGTGCAGATCGCGGGTTAGGCCGTGATTCTg                                                                                                                                                        >  2:584350/1‑251 (MQ=255)
                                                                                       gCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGc                                                                                                                                                      <  2:608555/250‑1 (MQ=255)
                                                                                        cctttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGa                                                                                                                                                    <  2:124441/248‑1 (MQ=255)
                                                                                         cGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTCTTACGACAAAACTTCTACGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGa                                                                                                                                                    >  1:539674/1‑250 (MQ=255)
                                                                                           ttCTGCGTTGGCGGAAACGGAAGTTGAGTATTACGACACAACTTCTCCGTCCCTCGACGTTGCTTTTCCGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAAATGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGAGaa                                                                                                                                                   >  2:400174/1‑249 (MQ=255)
                                                                                           ttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGaa                                                                                                                                                   >  1:486821/1‑249 (MQ=255)
                                                                                               gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCt                                                                                                                                             >  1:118171/1‑251 (MQ=255)
                                                                                                  cTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGt                                                                                                                                          >  1:500632/1‑251 (MQ=255)
                                                                                                    ggcggcAGCGGAAGTTGAGTATTACGACAAACCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTg                                                                                                                                        >  2:361159/1‑251 (MQ=255)
                                                                                                      cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                                                                                                       >  1:572832/1‑250 (MQ=255)
                                                                                                      cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGa                                                                                                                                       >  2:469775/1‑250 (MQ=255)
                                                                                                        gaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAg                                                                                                                                    <  1:179881/251‑1 (MQ=255)
                                                                                                        gaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCGCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAg                                                                                                                                    <  2:594982/251‑1 (MQ=255)
                                                                                                          agcggaagTTGAGAATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGAAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCAGGACCAC‑TACCACGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCACCTGATTTCGCATATGCGCTGGTGCTGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCg                                                                                                                                  >  1:605616/1‑251 (MQ=255)
                                                                                                            cggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGt                                                                                                                                 >  1:196694/1‑250 (MQ=255)
                                                                                                            cggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                                                                                                <  1:338578/251‑1 (MQ=255)
                                                                                                             ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                                                                                                >  1:455731/1‑250 (MQ=255)
                                                                                                                      gTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCACGCCGTGTTTTTGGCGACCGATCAGGTTGAAAGCGTAATGCAGCgg                                                                                                                       >  2:519533/1‑249 (MQ=255)
                                                                                                                       tATTACGACAAAACTTCTCCGTCCATCGCCGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGACCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCCGGCCGCGATTCTTGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTa                                                                                                                      >  2:175888/1‑250 (MQ=255)
                                                                                                                       tATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAt                                                                                                                     >  1:312301/1‑251 (MQ=255)
                                                                                                                           aCGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                                                                                                 <  1:156490/251‑1 (MQ=255)
                                                                                                                           aCGACAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                                                                                                 >  1:14834/1‑251 (MQ=255)
                                                                                                                               cAAAACTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                                                                                                              >  1:552464/1‑250 (MQ=255)
                                                                                                                                 aaaCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGAGCAGATCGCCGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                                                                                            >  1:8463/1‑250 (MQ=255)
                                                                                                                                 aaaCTTCTCCGTCCATCGACGTTGCGTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                                                                                                           <  2:8463/251‑1 (MQ=255)
                                                                                                                                   aCTTCTCCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGa                                                                                                         <  1:549816/251‑1 (MQ=255)
                                                                                                                                      tctcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAtt                                                                                                       <  1:481011/250‑1 (MQ=255)
                                                                                                                                        tcCGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTGGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCTGCGTCTCGGCGTGATCGATTac                                                                                                     >  2:7960/1‑250 (MQ=255)
                                                                                                                                         ccGTCCATCGACGTTGCTTTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGCGCAGCTCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTACTGAAGCGTATCGGCGTGGCCGGTTacac                                                                                                   >  2:54941/1‑251 (MQ=255)
                                                                                                                                          cGTCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                                                                                                  >  1:86887/1‑251 (MQ=255)
                                                                                                                                            tCCATCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTACTGCAGCGTATCGGCGTGACCGATTTCACCAt                                                                                                >  2:534495/1‑251 (MQ=255)
                                                                                                                                               aTCGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtct                                                                                             >  1:134832/1‑251 (MQ=255)
                                                                                                                                                 cGACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca                                                                                           <  1:469255/251‑2 (MQ=255)
                                                                                                                                                  gACGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcag                                                                                          <  2:572832/251‑3 (MQ=255)
                                                                                                                                                   aCGTTGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc                                                                                         >  1:279700/1‑248 (MQ=255)
                                                                                                                                                    cGTTGCTTTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGCGAAAAATCTGGATGAAAGCGTAATGCAGCGTATCGGAGTGACAAATTACACCAttataacac                                                                                        >  2:326224/1‑244 (MQ=255)
                                                                                                                                                      ttGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGAACAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACAGATTACACCATTCTCAGCACg                                                                                      >  1:275607/1‑251 (MQ=255)
                                                                                                                                                       tGCTTTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGAGACCGATTACACAATTCTCAGCACgg                                                                                     >  1:217517/1‑251 (MQ=255)
                                                                                                                                                             tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCCTTCTCAGCACGGTaaa                                                                                 >  1:467094/2‑249 (MQ=255)
                                                                                                                                                              cAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAgg                                                                              <  2:467094/251‑1 (MQ=255)
                                                                                                                                                                 gcggTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTATATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTa                                                                                                      <  1:565926/221‑1 (MQ=255)
                                                                                                                                                                   ggTCGAGCAGGAAGCACTGAAACCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTAGTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACTCCATTCTCAGCCCGGTAAAAGTTGCgg                                                                         <  2:405879/250‑1 (MQ=255)
                                                                                                                                                                    gTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATGGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCTTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGa                                                                        <  1:332217/251‑1 (MQ=255)
                                                                                                                                                                            ggATGCACTGAAAGCAAAAGTTGCCGGAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATGCTCAGCACGGTAAAAGGTGCGGAGCTTGAgt                                                                <  2:229941/251‑2 (MQ=255)
                                                                                                                                                                                 cACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCCGCACGGTAAAAGGTGCGGAGCTTGAGttgctg                                                           >  2:410250/1‑251 (MQ=255)
                                                                                                                                                                                  ccTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCATCACGGTAAAAGGTGCGGAGCTTGAGTtgctgc                                                          <  1:470229/250‑1 (MQ=255)
                                                                                                                                                                                      aaaGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCtt                                                      <  1:499036/251‑1 (MQ=255)
                                                                                                                                                                                         ggaaaagttgttgTAAGCAACGTAAACGGCACAAGCTCGCTGGTG‑ATTTGGGACACTCA‑GTCGTGGACTCTGCCTGCGAAACGCGCAATCTCTATTGTACCGGATTTCGACTATGCGCTGGTGCAGATCGACGGTAAGGCCGGGATTCTGGCGAAAGATCTGGTTGAAAGCGTAAGGCAGCTTATCGGCGTGACCGATTACACCAGGCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGATGCGCtgtcc                                                   <  2:305054/239‑5 (MQ=255)
                                                                                                                                                                                                  gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGGCCGATTACACCATTCTCAGAACGGTAAAAGGTGCGGAGCTTGAGTTGTTGCGCTTTACCCATCCGtt                                          >  2:406545/1‑251 (MQ=255)
                                                                                                                                                                                                  gCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑GACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGt                                           <  2:541161/250‑1 (MQ=255)
                                                                                                                                                                                                       aaGCAACGTTAACAGCCCAATCTCGCTTGTT‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTTAAAGCGTAATGCTGCTTATCTGCGTGACCGATTACACCATGCTCAGCTCGGTAAAAGGTGCGGATCTTGTGTTGCTGCGCTTTACCCATCCGTTTATgg                                     <  2:564774/251‑1 (MQ=255)
                                                                                                                                                                                                        aGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATggg                                    >  2:90286/1‑251 (MQ=255)
                                                                                                                                                                                                         acAACGTTTACGGCGCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCGGCGAGCCGCGCAATCTCTAGTGCTCCTGATTTCGTCTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTACAAGGTGCGGATCTTGAGTTGCTGCGCGTTACCCATCCGTTTATGGGc                                   <  2:379661/250‑1 (MQ=255)
                                                                                                                                                                                                           aaCGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCtt                                 >  1:377206/1‑251 (MQ=255)
                                                                                                                                                                                                             cGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCg                               <  2:467737/251‑1 (MQ=255)
                                                                                                                                                                                                              gttataggCCCAATCTCGTTGGTG‑ATCTTGACCAC‑CTCGCCCTGGACTCTGCTTGCTAACCGCACAATCTCTATCGTACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTTGTTGAAAGTGTATTGCAGCGTATCGGCGCGACCGATTACACCATTCTCAGCACGGTAAAAGTTTCGTAGCTTGAGTTGCTGCGCTTTCCCCATCCGTTTATGGGCTTcta                              <  1:602154/245‑3 (MQ=255)
                                                                                                                                                                                                              gTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGa                              <  1:602138/251‑1 (MQ=255)
                                                                                                                                                                                                                    ggCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTcc                        <  1:361159/251‑1 (MQ=255)
                                                                                                                                                                                                                     gCCCAATCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCAAGGTAAAAGGTGCGGAGCTTGATGTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCg                       >  2:616991/1‑251 (MQ=255)
                                                                                                                                                                                                                          aTCTCGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTTAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAAt                  >  1:163618/1‑251 (MQ=255)
                                                                                                                                                                                                                              cGCTGGTG‑ATCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTc              >  1:441006/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ggtgaTCTGGACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGc           <  2:455731/246‑1 (MQ=255)
                                                                                                                                                                                                                                     ggaTCTGGACCCC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGAt        <  2:25533/249‑1 (MQ=255)
                                                                                                                                                                                                                                           ggACCAC‑TACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAGGTGCGGAGCTTGAGTTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGtt  <  1:342290/251‑1 (MQ=255)
                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                   
AAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCTTTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCAC‑CACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGGTGCGGAGCTTGAGCTGCTGCGCTTTACCCATCCGTTTATGGGCTTCGACGTTCCGGCAATCCTCGGCGATCACGTT  >  NC_000913/22875‑23359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑