BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,038	A→G	A216A (GCA→GCG)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,038	0	A	G	100.0%	349.8 / NA	113	A216A (GCA→GCG)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (72/41); total (72/41)

Only 100 of 113 total aligned reads displayed.

TGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGG  >  NC_000913/22792‑23283

tGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCg                                                                                                                                                                                                                                                    >  1:560331/1‑251 (MQ=255)

 gACGGTCAACGCAAAGAATTTATCCGTCTGGGCGTGCTGGGCGACTGGTAGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTTCGTCCATCGACGTTGCT‑TTTCAGGCGGTCg                                                                                                                                                                                                                                                    <  1:462136/250‑1 (MQ=255)

   cGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATc                                                                                                                                                                                                                                                 <  1:524925/251‑1 (MQ=255)

    ggTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCa                                                                                                                                                                                                                                                <  1:29104/251‑1 (MQ=255)

     gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAg                                                                                                                                                                                                                                               <  1:17400/251‑1 (MQ=255)

     gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGAACCCGTACCTGACCATGGACTTCAAAACTGAAGACAACATCATCAGCGCGCTGAGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGACGCGGCAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATc                                                                                                                                                                                                                                                 >  1:621505/1‑249 (MQ=255)

            cAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCAc                                                                                                                                                                                                                                        >  1:435012/1‑251 (MQ=255)

             acaGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGGACAATGGTGCGTTGACTGCCGTTCTGCGCTTGCGGTAGAGGAAGTTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCATGATGccct                                                                                                                                                                                                                                       >  2:547934/3‑248 (MQ=255)

             aaaGACTTTAACCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACt                                                                                                                                                                                                                                       <  2:218403/251‑1 (MQ=255)

                gACTTTATCCGTCTGGGCGTGCGGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                                                                                                                                    <  2:493253/251‑1 (MQ=255)

                  cTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                                                                                                                                    >  2:602138/1‑249 (MQ=255)

                    ttATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGc                                                                                                                                                                                                                                 >  1:124441/1‑250 (MQ=255)

                      aTCCGTCTGGGCGTGCTTGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAAAATCATCCGCGCGCTGGGCAAAATCAGCGGCACCGGTCACCTGCAACAAGGCGAGAAGCCGGTACACTGGCCAGTTGACTGCCATTCTGCGCTGGCGGAAGCGGAAGGTGCATATTACGACAAAACTTCTCCGTACATCGCCGTTGCT‑TTTCAGGCGGCCGATCATGAtcccctgaccgcaa                                                                                                                                                                                                                               >  1:305255/1‑237 (MQ=255)

                        ccGTCGGGGCGTGATGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAAt                                                                                                                                                                                                                            <  2:549789/251‑1 (MQ=255)

                              aggCGTGCTGGGCGCTTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCGGCACCAAGGCGCGACGCCGGTACCCTGGTGCGTTGACTGCAGCTCTGCGCTGGCGGAAGCGGAAGATGAGGATTACGACAAAACTCCTCCGGCCATCGCCGTTGCTGTTAC‑GGCGGTCGATCAGGATGCACTGAACGCAACATTTGCCg                                                                                                                                                                                                                      >  2:538515/2‑251 (MQ=255)

                                           gACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTa                                                                                                                                                                                                          >  1:472645/1‑250 (MQ=255)

                                                      cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                                                                                                                              <  1:450226/251‑1 (MQ=255)

                                                      cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                                                                                                                              >  1:14345/1‑251 (MQ=255)

                                                      cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGACACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                                                                                                                              >  1:100799/1‑251 (MQ=255)

                                                        cGTACCGGACCATGGCCTTCAAAACTGAAGCCAACATCATCCGTGCGTTGGGCAAAATCATCGGCAACGGTCACCTGAACAAAGGCGCGAAGTCGGTACACTGGTTCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGGCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGGATGTACTGAAAGCAAAAGGTGCCGTAATCAACGTTAACGGCCCTATCTCg                                                                                                                                                                                            <  2:226849/251‑1 (MQ=255)

                                                           aCCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTg                                                                                                                                                                                         <  2:196043/251‑1 (MQ=255)

                                                             cTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGt                                                                                                                                                                                       >  2:312986/1‑251 (MQ=255)

                                                               gACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                                      >  1:217460/1‑249 (MQ=255)

                                                               gACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                                      >  2:178605/1‑249 (MQ=255)

                                                               gACCATGAACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                                      >  1:467737/1‑249 (MQ=255)

                                                                  cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCt                                                                                                                                                                                 >  2:470229/1‑251 (MQ=255)

                                                                  cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCt                                                                                                                                                                                 >  1:608555/1‑251 (MQ=255)

                                                                     ggACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCAGCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTgg                                                                                                                                                                               <  1:44085/250‑1 (MQ=255)

                                                                      gACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGac                                                                                                                                                                             <  2:457221/251‑1 (MQ=255)

                                                                           cAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGaccacta                                                                                                                                                                        <  2:507242/251‑3 (MQ=255)

                                                                                      ccAACATCATCCGAGCGCTGGGAAAAATCATCGGCAAAGGCCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGAG‑ATCTGGACCACTACGCCGTGGAct                                                                                                                                                             >  2:297040/1‑251 (MQ=255)

                                                                                        aaCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGActct                                                                                                                                                           >  1:488819/1‑251 (MQ=255)

                                                                                         aCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTctg                                                                                                                                                          >  2:469255/1‑251 (MQ=255)

                                                                                          catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCCAAATTCGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCACTACGCCGTGGACTctgc                                                                                                                                                         >  2:248470/1‑251 (MQ=255)

                                                                                          catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTctgc                                                                                                                                                         >  1:166474/1‑251 (MQ=255)

                                                                                                   cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACACAGGCGCGAAGCCGGAACACTGGTGCGTTGCCTGCCGTTCTGCGCTGACGGAAGCTGAAGTTAAGGATTACGACAAACCTTCTCCGTCCATCGACGGTGCT‑TTTCAGGCGGTCAATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCTTTACCGGCCCAATCTCACTG‑TAGATCTTGACCACTACGCCATgggttcacccttcagacc                                                                                                                                                >  1:305054/1‑235 (MQ=255)

                                                                                                   cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAAcc                                                                                                                                                >  2:140942/1‑251 (MQ=255)

                                                                                                           gCAAAATCATCGTCAACGGTCACCTGCACAAATGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCGGCCTGCGAACCGCGCAAtc                                                                                                                                        <  2:415624/251‑1 (MQ=255)

                                                                                                                atcatcGGCAACGGTCACCTGCACAAAGGCGCGCAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAACGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGCACCACGCAACCTCTAt                                                                                                                                   >  2:462002/1‑251 (MQ=255)

                                                                                                                     cGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAc                                                                                                                              >  2:481011/1‑251 (MQ=255)

                                                                                                                          aCGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAt                                                                                                                         >  1:554175/1‑251 (MQ=255)

                                                                                                                           cGGTCACCTGCACAAAGGCGCGAAGCCGGAACACTGGTGCGTTGACTGCAGTTCTGCGCTGGCGGCAGCGGAAGTTGCGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGCCGATCCGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAtt                                                                                                                        >  1:572363/1‑251 (MQ=255)

                                                                                                                            gggcgccTGCTCAAAGGCGTGAAGCCGGTACACTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGGACCACTACGCCGTGGTCTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGAttt                                                                                                                       <  2:615824/246‑1 (MQ=255)

                                                                                                                             gTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTc                                                                                                                      >  2:156490/1‑251 (MQ=255)

                                                                                                                                aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGCGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCgcc                                                                                                                   >  1:307181/1‑249 (MQ=255)

                                                                                                                                  cggcccaacggCGCGAAGCCGGTGCTCTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTGCTCCGTCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGTACCACTACGCCTTGGACTCTGCCTGCGAACCGCGCACTCTCTATTGCACCTGATGTCGACTa                                                                                                                 <  2:82847/242‑1 (MQ=255)

                                                                                                                                      acaAAGGCGTGAGGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCCTCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCCCTATTGACCCAGATTTCGCCTATgcg                                                                                                             >  2:378150/1‑251 (MQ=255)

                                                                                                                                       caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgc                                                                                                            >  2:549816/1‑251 (MQ=255)

                                                                                                                                        aaaGGCGCGAAGCCGGTACACTGATGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCAATCGACGTTGCT‑TTTCAGGGGGTCGATCGGGCTGCATGGAAAGCAAAATTTACCGTAAGCACCGTTAACGGCCCAACCTCGATGGGG‑ATCCGGACCCCCACGAATGGGACTTTGCCTCCGAACCGCACAATCTCTATTGCACTTGATTTCGACTATgcact                                                                                                           >  1:140196/1‑248 (MQ=255)

                                                                                                                                          aGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTTAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTgg                                                                                                         <  2:375086/251‑1 (MQ=255)

                                                                                                                                            gcgcGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTCCGACAAAACTTATCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGGTGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTg                                                                                                       >  1:405879/1‑251 (MQ=255)

                                                                                                                                                 aagccggtacccTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt                                                                                                  <  2:504190/240‑1 (MQ=255)

                                                                                                                                                    ccggtacACTGGTGCTGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCg                                                                                                <  2:444735/244‑1 (MQ=255)

                                                                                                                                                     cgttacaatGGTGCGTTGACTGCCGTTCTGTGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTGAACGGCCCAATTTCGCTGGTG‑ATCTGGCCCACTACGCCGTGGCCTCTGCCTGCGACCCGCGCACTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGAc                                                                                              <  1:515373/243‑1 (MQ=255)

                                                                                                                                                      ggtacacggggttgttgacgtccGTGCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCATGCGGTCGATCAGGATGCGCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTTGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGTAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGTGCAGATCGACg                                                                                             <  2:80787/230‑1 (MQ=255)

                                                                                                                                                          cACTGGTGCGGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGAGCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCa                                                                                         <  2:14345/251‑1 (MQ=255)

                                                                                                                                                            cTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAgg                                                                                       >  1:25533/1‑251 (MQ=255)

                                                                                                                                                               gTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCg                                                                                    >  2:338578/1‑251 (MQ=255)

                                                                                                                                                                 gCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                                   <  1:153609/250‑1 (MQ=255)

                                                                                                                                                                   gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                                >  1:541161/1‑251 (MQ=255)

                                                                                                                                                                   gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                                >  1:367818/1‑251 (MQ=255)

                                                                                                                                                                    ttGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAtt                                                                               >  2:439812/1‑251 (MQ=255)

                                                                                                                                                                       aCTGCCGTTCAGAGCTCGCGGAAGCGGAAGGTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTACT‑TTTCAGGCGGTCGCACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTCACGGCCCAATATCGCTGGTG‑ATCTGGAAAACTCCGCCGTGGACTCTGCCTGAGAACCGCGCAATCTCTCTTTCACCTGATTTCGACTATGCGCTGGTGCAGATCGCGGGTTAGGCCGTGATTCTg                                                                            >  2:584350/1‑251 (MQ=255)

                                                                                                                                                                          gCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGc                                                                          <  2:608555/250‑1 (MQ=255)

                                                                                                                                                                            cGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTCTTACGACAAAACTTCTACGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGa                                                                        >  1:539674/1‑250 (MQ=255)

                                                                                                                                                                            cGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGa                                                                        >  2:449906/1‑250 (MQ=255)

                                                                                                                                                                              ttCTGCGTTGGCGGAAACGGAAGTTGAGTATTACGACACAACTTCTCCGTCCCTCGACGTTGCT‑TTTCCGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAAATGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGAGaa                                                                       >  2:400174/1‑249 (MQ=255)

                                                                                                                                                                              ttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGaa                                                                       >  1:486821/1‑249 (MQ=255)

                                                                                                                                                                               tCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGTGG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAg                                                                     <  1:417220/250‑1 (MQ=255)

                                                                                                                                                                                  gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCt                                                                 >  1:118171/1‑251 (MQ=255)

                                                                                                                                                                                     cTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGt                                                              >  1:500632/1‑251 (MQ=255)

                                                                                                                                                                                       ggcggcAGCGGAAGTTGAGTATTACGACAAACCTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTg                                                            >  2:361159/1‑251 (MQ=255)

                                                                                                                                                                                         cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                           >  1:572832/1‑250 (MQ=255)

                                                                                                                                                                                         cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGa                                                           >  2:469775/1‑250 (MQ=255)

                                                                                                                                                                                           gaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAg                                                        <  1:179881/251‑1 (MQ=255)

                                                                                                                                                                                             agcggaagTTGAGAATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGCTGCACTGAAAGCAAAATTTGCCGAAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCAGGACCACTACCACGTGGACTCTGCCTGCGCACCGCGCAATCTCTATTGCACCTGATTTCGCATATGCGCTGGTGCTGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCg                                                      >  1:605616/1‑251 (MQ=255)

                                                                                                                                                                                               cggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGt                                                     >  1:196694/1‑250 (MQ=255)

                                                                                                                                                                                               cggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                    <  1:338578/251‑1 (MQ=255)

                                                                                                                                                                                                ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTa                                                    >  1:455731/1‑250 (MQ=255)

                                                                                                                                                                                                          tATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGACCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCCGGCCGCGATTCTTGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTa                                          >  2:175888/1‑250 (MQ=255)

                                                                                                                                                                                                          tATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAt                                         >  1:312301/1‑251 (MQ=255)

                                                                                                                                                                                                              aCGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                     >  1:14834/1‑251 (MQ=255)

                                                                                                                                                                                                              aCGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                                     <  1:156490/251‑1 (MQ=255)

                                                                                                                                                                                                                  cAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                                  >  1:552464/1‑250 (MQ=255)

                                                                                                                                                                                                                    aaaCTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGAGCAGATCGCCGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                                >  1:8463/1‑250 (MQ=255)

                                                                                                                                                                                                                    aaaCTTCTCCGTCCATCGACGTTGCG‑TTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                               <  2:8463/251‑1 (MQ=255)

                                                                                                                                                                                                                      aCTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGa                             <  1:549816/251‑1 (MQ=255)

                                                                                                                                                                                                                         tctcCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAtt                           <  1:481011/250‑1 (MQ=255)

                                                                                                                                                                                                                           tcCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTGGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCTGCGTCTCGGCGTGATCGATTac                         >  2:7960/1‑250 (MQ=255)

                                                                                                                                                                                                                            ccGTCCATCGACGTTGCT‑TTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGCGCAGCTCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTACTGAAGCGTATCGGCGTGGCCGGTTacac                       >  2:54941/1‑251 (MQ=255)

                                                                                                                                                                                                                             cGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc                      >  1:86887/1‑251 (MQ=255)

                                                                                                                                                                                                                               tCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTACTGCAGCGTATCGGCGTGACCGATTTCACCAt                    >  2:534495/1‑251 (MQ=255)

                                                                                                                                                                                                                                  aTCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtct                 >  1:134832/1‑251 (MQ=255)

                                                                                                                                                                                                                                    cGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctca               <  1:469255/251‑2 (MQ=255)

                                                                                                                                                                                                                                     gACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcag              <  2:572832/251‑3 (MQ=255)

                                                                                                                                                                                                                                      aCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc             >  1:279700/1‑248 (MQ=255)

                                                                                                                                                                                                                                       cGTTGCT‑TTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGCGAAAAATCTGGATGAAAGCGTAATGCAGCGTATCGGAGTGACAAATTACACCAttataacac            >  2:326224/1‑244 (MQ=255)

                                                                                                                                                                                                                                          tGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGAGACCGATTACACAATTCTCAGCACgg         >  1:217517/1‑251 (MQ=255)

                                                                                                                                                                                                                                                 tcAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAACGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGCTTACACCCTTCTCAGCACGGTaaa     >  1:467094/2‑249 (MQ=255)

                                                                                                                                                                                                                                                  cAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCAGCACGGTAAAAgg  <  2:467094/251‑1 (MQ=255)

TGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGGTAAAAGG  >  NC_000913/22792‑23283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`