BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	23,032	C→T	F214F (TTC→TTT)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	23,032	0	C	T	97.5%	373.5 / NA	119	F214F (TTC→TTT)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (73/42); minor base G (1/2); total (75/44)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

CCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGG  >  NC_000913/22786‑23276

ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACACAGGAGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTTCGCTGGCGGAAGCGGACGTTGAGTATTACGCCACAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAgg                                                                                                                                                                                                                                                   >  1:629772/1‑251 (MQ=255)

ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAgg                                                                                                                                                                                                                                                   >  1:573239/1‑251 (MQ=255)

ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAgg                                                                                                                                                                                                                                                   <  1:183824/251‑1 (MQ=255)

ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAgg                                                                                                                                                                                                                                                   >  2:499036/1‑251 (MQ=255)

    gtttaCGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGTAAGTTGAGTATTACTACAAAACTGCTCCGTCCATCGACGTTGCT‑TTTCAGGcggt                                                                                                                                                                                                                                               <  2:373043/247‑4 (MQ=255)

    gTTGACGGTTAACGCAAAGACTTTATCCGGCTGGGCGTGCTGGGCGACTGGTCGCCCCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGcggt                                                                                                                                                                                                                                               <  2:377374/251‑4 (MQ=255)

      tGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCg                                                                                                                                                                                                                                             >  1:560331/1‑251 (MQ=255)

      tGACGGTCAACGCAAAGACTTGATCCGTCGGGGTGTGCCGGGCGATTGGGTGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCACCCGCGCCCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACTCTGGTTCGTGGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGcggtt                                                                                                                                                                                                                                              <  2:22921/250‑5 (MQ=255)

       gACGGTCAACGCAAAGAATTTATCCGTCTGGGCGTGCTGGGCGACTGGTAGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTTCGTCCATCGACGTTGCT‑TTTCAGGCGGTCg                                                                                                                                                                                                                                             <  1:462136/250‑1 (MQ=255)

         cGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATc                                                                                                                                                                                                                                          <  1:524925/251‑1 (MQ=255)

          ggTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCa                                                                                                                                                                                                                                         <  1:29104/251‑1 (MQ=255)

           gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAg                                                                                                                                                                                                                                        <  1:17400/251‑1 (MQ=255)

           gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGAACCCGTACCTGACCATGGACTTCAAAACTGAAGACAACATCATCAGCGCGCTGAGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGACGCGGCAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATc                                                                                                                                                                                                                                          >  1:621505/1‑249 (MQ=255)

                  cAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCAc                                                                                                                                                                                                                                 >  1:435012/1‑251 (MQ=255)

                   acaGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGGACAATGGTGCGTTGACTGCCGTTCTGCGCTTGCGGTAGAGGAAGTTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCATGATGccct                                                                                                                                                                                                                                >  2:547934/3‑248 (MQ=255)

                   aaaGACTTTAACCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACt                                                                                                                                                                                                                                <  2:218403/251‑1 (MQ=255)

                      gACTTTATCCGTCTGGGCGTGCGGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                                                                                                                             <  2:493253/251‑1 (MQ=255)

                        cTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                                                                                                                             >  2:602138/1‑249 (MQ=255)

                          ttATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGc                                                                                                                                                                                                                          >  1:124441/1‑250 (MQ=255)

                            aTCCGTCTGGGCGTGCTTGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAAAATCATCCGCGCGCTGGGCAAAATCAGCGGCACCGGTCACCTGCAACAAGGCGAGAAGCCGGTACACTGGCCAGTTGACTGCCATTCTGCGCTGGCGGAAGCGGAAGGTGCATATTACGACAAAACTTCTCCGTACATCGCCGTTGCT‑TTTCAGGCGGCCGATCATGAtcccctgaccgcaa                                                                                                                                                                                                                        >  1:305255/1‑237 (MQ=255)

                                    gggCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCg                                                                                                                                                                                                               >  1:154280/1‑251 (MQ=255)

                                    aggCGTGCTGGGCGCTTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCGGCACCAAGGCGCGACGCCGGTACCCTGGTGCGTTGACTGCAGCTCTGCGCTGGCGGAAGCGGAAGATGAGGATTACGACAAAACTCCTCCGGCCATCGCCGTTGCTGTTAC‑GGCGGTCGATCAGGATGCACTGAACGCAACATTTGCCg                                                                                                                                                                                                               >  2:538515/2‑251 (MQ=255)

                                                 gACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTa                                                                                                                                                                                                   >  1:472645/1‑250 (MQ=255)

                                                            cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                                                                                                                       >  1:14345/1‑251 (MQ=255)

                                                            cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                                                                                                                       <  1:450226/251‑1 (MQ=255)

                                                            cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGACACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                                                                                                                       >  1:100799/1‑251 (MQ=255)

                                                              cGTACCGGACCATGGCCTTCAAAACTGAAGCCAACATCATCCGTGCGTTGGGCAAAATCATCGGCAACGGTCACCTGAACAAAGGCGCGAAGTCGGTACACTGGTTCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGGCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGGATGTACTGAAAGCAAAAGGTGCCGTAATCAACGTTAACGGCCCTATCTCg                                                                                                                                                                                     <  2:226849/251‑1 (MQ=255)

                                                                 aCCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTg                                                                                                                                                                                  <  2:196043/251‑1 (MQ=255)

                                                                   cTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGt                                                                                                                                                                                >  2:312986/1‑251 (MQ=255)

                                                                     gACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                               >  2:178605/1‑249 (MQ=255)

                                                                     gACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                               >  1:217460/1‑249 (MQ=255)

                                                                     gACCATGAACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                                                                                                               >  1:467737/1‑249 (MQ=255)

                                                                        cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCt                                                                                                                                                                          >  2:470229/1‑251 (MQ=255)

                                                                        cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCt                                                                                                                                                                          >  1:608555/1‑251 (MQ=255)

                                                                            gACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGac                                                                                                                                                                      <  2:457221/251‑1 (MQ=255)

                                                                                              aaCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGActct                                                                                                                                                    >  1:488819/1‑251 (MQ=255)

                                                                                                catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCCAAATTCGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCACTACGCCGTGGACTctgc                                                                                                                                                  >  2:248470/1‑251 (MQ=255)

                                                                                                catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTctgc                                                                                                                                                  >  1:166474/1‑251 (MQ=255)

                                                                                                         cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACACAGGCGCGAAGCCGGAACACTGGTGCGTTGCCTGCCGTTCTGCGCTGACGGAAGCTGAAGTTAAGGATTACGACAAACCTTCTCCGTCCATCGACGGTGCT‑TTTCAGGCGGTCAATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCTTTACCGGCCCAATCTCACTG‑TAGATCTTGACCACTACGCCATgggttcacccttcagacc                                                                                                                                         >  1:305054/1‑235 (MQ=255)

                                                                                                         cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAAcc                                                                                                                                         >  2:140942/1‑251 (MQ=255)

                                                                                                         cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGATCGATCAGGATGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAAcc                                                                                                                                         >  1:375086/1‑251 (MQ=255)

                                                                                                                 gCAAAATCATCGTCAACGGTCACCTGCACAAATGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCGGCCTGCGAACCGCGCAAtc                                                                                                                                 <  2:415624/251‑1 (MQ=255)

                                                                                                                      atcatcGGCAACGGTCACCTGCACAAAGGCGCGCAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAACGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGCACCACGCAACCTCTAt                                                                                                                            >  2:462002/1‑251 (MQ=255)

                                                                                                                          tcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGATGGCGGAAGCGGAAGATGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAACTATATTGc                                                                                                                         >  1:564774/1‑250 (MQ=255)

                                                                                                                           cGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAc                                                                                                                       >  2:481011/1‑251 (MQ=255)

                                                                                                                                 cGGTCACCTGCACAAAGGCGCGAAGCCGGAACACTGGTGCGTTGACTGCAGTTCTGCGCTGGCGGCAGCGGAAGTTGCGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGCCGATCCGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAtt                                                                                                                 >  1:572363/1‑251 (MQ=255)

                                                                                                                                  gggcgccTGCTCAAAGGCGTGAAGCCGGTACACTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGGACCACTACGCCGTGGTCTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGAttt                                                                                                                <  2:615824/246‑1 (MQ=255)

                                                                                                                                   gTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTc                                                                                                               >  2:156490/1‑251 (MQ=255)

                                                                                                                                    tCACCTTCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACGGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCa                                                                                                                        <  2:463459/241‑1 (MQ=255)

                                                                                                                                    tCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCa                                                                                                                        >  1:463459/1‑241 (MQ=255)

                                                                                                                                      aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGCGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCgcc                                                                                                            >  1:307181/1‑249 (MQ=255)

                                                                                                                                        cggcccaacggCGCGAAGCCGGTGCTCTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTGCTCCGTCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGTACCACTACGCCTTGGACTCTGCCTGCGAACCGCGCACTCTCTATTGCACCTGATGTCGACTa                                                                                                          <  2:82847/242‑1 (MQ=255)

                                                                                                                                            acaAAGGCGTGAGGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCCTCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCCCTATTGACCCAGATTTCGCCTATgcg                                                                                                      >  2:378150/1‑251 (MQ=255)

                                                                                                                                             caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgc                                                                                                     >  2:549816/1‑251 (MQ=255)

                                                                                                                                              aaaGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCGGCGAACCGCGGAATCTCTAGTGCTCTTGATTTCGACTATGCGCt                                                                                                    <  2:399481/251‑1 (MQ=255)

                                                                                                                                              aaaGGCGCGAAGCCGGTACACTGATGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCAATCGACGTTGCT‑TTTCAGGGGGTCGATCGGGCTGCATGGAAAGCAAAATTTACCGTAAGCACCGTTAACGGCCCAACCTCGATGGGG‑ATCCGGACCCCCACGAATGGGACTTTGCCTCCGAACCGCACAATCTCTATTGCACTTGATTTCGACTATgcact                                                                                                    >  1:140196/1‑248 (MQ=255)

                                                                                                                                                  gcgcGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTCCGACAAAACTTATCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGGTGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTg                                                                                                >  1:405879/1‑251 (MQ=255)

                                                                                                                                                       aagccggtacccTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGAt                                                                                           <  2:504190/240‑1 (MQ=255)

                                                                                                                                                          ccggtacACTGGTGCTGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCg                                                                                         <  2:444735/244‑1 (MQ=255)

                                                                                                                                                           cgttacaatGGTGCGTTGACTGCCGTTCTGTGCTTGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTGAACGGCCCAATTTCGCTGGTG‑ATCTGGCCCACTACGCCGTGGCCTCTGCCTGCGACCCGCGCACTCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGAc                                                                                       <  1:515373/243‑1 (MQ=255)

                                                                                                                                                            ggtacacggggttgttgacgtccGTGCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCATGCGGTCGATCAGGATGCGCTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTTGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGTAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGTGCAGATCGACg                                                                                      <  2:80787/230‑1 (MQ=255)

                                                                                                                                                               ccACTGCTGCGTTGCCTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCGCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGAGCAGGATGCTCGGAAAGCAAAATCTGCCGTAAGCAGCGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACGACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGTACCCGATTTCCGCTATGCGCTGGTGGTGATCGACGGTc                                                                                   <  2:232793/250‑1 (MQ=255)

                                                                                                                                                                cACTGGTGCGGTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGAGCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCa                                                                                  <  2:14345/251‑1 (MQ=255)

                                                                                                                                                                  cTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAgg                                                                                >  1:25533/1‑251 (MQ=255)

                                                                                                                                                                     gTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCg                                                                             >  2:338578/1‑251 (MQ=255)

                                                                                                                                                                       gCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGt                                                                            <  1:153609/250‑1 (MQ=255)

                                                                                                                                                                         gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                         >  1:541161/1‑251 (MQ=255)

                                                                                                                                                                         gTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGAt                                                                         >  1:367818/1‑251 (MQ=255)

                                                                                                                                                                             aCTGCCGTTCAGAGCTCGCGGAAGCGGAAGGTGAGTATTAAGACAAAACTTCTCCGTCCATCGACGTTACT‑TTTCAGGCGGTCGCACAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTCACGGCCCAATATCGCTGGTG‑ATCTGGAAAACTCCGCCGTGGACTCTGCCTGAGAACCGCGCAATCTCTCTTTCACCTGATTTCGACTATGCGCTGGTGCAGATCGCGGGTTAGGCCGTGATTCTg                                                                     >  2:584350/1‑251 (MQ=255)

                                                                                                                                                                                  cGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTCTTACGACAAAACTTCTACGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGCCGGTCAGGCCGTGATTCTGGCGa                                                                 >  1:539674/1‑250 (MQ=255)

                                                                                                                                                                                  cGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGa                                                                 >  2:449906/1‑250 (MQ=255)

                                                                                                                                                                                    ttCTGCGTTGGCGGAAACGGAAGTTGAGTATTACGACACAACTTCTCCGTCCCTCGACGTTGCT‑TTTCCGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAAATGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGCTTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGAGaa                                                                >  2:400174/1‑249 (MQ=255)

                                                                                                                                                                                    ttCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGaa                                                                >  1:486821/1‑249 (MQ=255)

                                                                                                                                                                                     tCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGTGG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTGCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAg                                                              <  1:417220/250‑1 (MQ=255)

                                                                                                                                                                                        gcgcTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCt                                                          >  1:118171/1‑251 (MQ=255)

                                                                                                                                                                                           cTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGt                                                       >  1:500632/1‑251 (MQ=255)

                                                                                                                                                                                             ggcggcAGCGGAAGTTGAGTATTACGACAAACCTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTg                                                     >  2:361159/1‑251 (MQ=255)

                                                                                                                                                                                               cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGa                                                    >  1:572832/1‑250 (MQ=255)

                                                                                                                                                                                               cggaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGa                                                    >  2:469775/1‑250 (MQ=255)

                                                                                                                                                                                                 gaagcggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCGCGCTGGTG‑ATCTGGACCACTACGCCGTGGACGCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAg                                                 <  2:594982/251‑1 (MQ=255)

                                                                                                                                                                                                     cggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGt                                              >  1:196694/1‑250 (MQ=255)

                                                                                                                                                                                                      ggaagTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTa                                             >  1:455731/1‑250 (MQ=255)

                                                                                                                                                                                                               gTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGCCGGTCACGCCGTGTTTTTGGCGACCGATCAGGTTGAAAGCGTAATGCAGCgg                                    >  2:519533/1‑249 (MQ=255)

                                                                                                                                                                                                                tATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGACCCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCCGATCGACGGTCCGGCCGCGATTCTTGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTa                                   >  2:175888/1‑250 (MQ=255)

                                                                                                                                                                                                                tATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTAt                                  >  1:312301/1‑251 (MQ=255)

                                                                                                                                                                                                                    aCGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGc                              <  1:156490/251‑1 (MQ=255)

                                                                                                                                                                                                                        cAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTg                           >  1:552464/1‑250 (MQ=255)

                                                                                                                                                                                                                          aaaCTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGTCTATGCGCTGGAGCAGATCGCCGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAc                         >  1:8463/1‑250 (MQ=255)

                                                                                                                                                                                                                          aaaCTTCTCCGTCCATCGACGTTGCG‑TTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGAcc                        <  2:8463/251‑1 (MQ=255)

                                                                                                                                                                                                                            aCTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGa                      <  1:549816/251‑1 (MQ=255)

                                                                                                                                                                                                                               tctcCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGAtt                    <  1:481011/250‑1 (MQ=255)

                                                                                                                                                                                                                                 tcCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTGGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGACAGATCTGGTTGAAAGCGTAATGCTGCGTCTCGGCGTGATCGATTac                  >  2:7960/1‑250 (MQ=255)

                                                                                                                                                                                                                                  ccGTCCATCGACGTTGCT‑TTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGCGCAGCTCGACGGTCAGGCCGTGATTCTGGCGCAAGATCTGGTTGAAAGCGTACTGAAGCGTATCGGCGTGGCCGGTTacac                >  2:54941/1‑251 (MQ=255)

                                                                                                                                                                                                                                   cGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACAcc               >  1:86887/1‑251 (MQ=255)

                                                                                                                                                                                                                                     tCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTACTGCAGCGTATCGGCGTGACCGATTTCACCAt             >  2:534495/1‑251 (MQ=255)

                                                                                                                                                                                                                                        aTCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtct          >  1:134832/1‑251 (MQ=255)

                                                                                                                                                                                                                                           gACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcag       <  2:572832/251‑3 (MQ=255)

                                                                                                                                                                                                                                            aCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGTAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATtctcagc      >  1:279700/1‑248 (MQ=255)

                                                                                                                                                                                                                                             cGTTGCT‑TTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGCTTCTGGCGAAAAATCTGGATGAAAGCGTAATGCAGCGTATCGGAGTGACAAATTACACCAttataacac     >  2:326224/1‑244 (MQ=255)

                                                                                                                                                                                                                                                tGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGAGACCGATTACACAATTCTCAGCACgg  >  1:217517/1‑251 (MQ=255)

CCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTGCAGATCGACGGTCAGGCCGTGATTCTGGCGAAAGATCTGGTTGAAAGCGTAATGCAGCGTATCGGCGTGACCGATTACACCATTCTCGGCACGG  >  NC_000913/22786‑23276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`