Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,945	T→A	V185V (GTT→GTA)	ileS →	isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,945	0	T	A	97.9%	267.9 / NA	94	V185V (GTT→GTA)	ileS	isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (55/36);  minor base C (2/0);  total (57/37)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTG  >  NC_000913/22708‑23182                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                cGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGtt                                                                                                                                                                                                                                     <  1:388642/250‑1 (MQ=255)    gCAAGAATACGGTAAGCCGGGAGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACt                                                                                                                                                                                                                                 >  1:503557/1‑251 (MQ=255)       aGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGcc                                                                                                                                                                                                                              >  1:229941/1‑251 (MQ=255)          aTACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGtt                                                                                                                                                                                                                           >  1:444735/1‑251 (MQ=255)            aCGGTAAGCCGGGTGAGAAATTCCCCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTGGACTGCCGTTc                                                                                                                                                                                                                          <  2:387839/250‑1 (MQ=255)             cGGTAAGACAGGTGAGAAATTAACCGCCGCCGAGCTCCGCGACAAGCGCCGCGAATACGCGGCGACCCAGGCTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTAAAAACTGAAGCAAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTCCACTGGTGCGTAGACTGCCGTTCTg                                                                                                                                                                                                                        >  2:409786/1‑251 (MQ=255)              gggaggCCGGGTGAGAAGTGCGCCGCCGCCGATTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACGGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCACCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCTGTACACTTTTGCGTTGACTGCCGTTCTgc                                                                                                                                                                                                                       <  2:212387/246‑1 (MQ=255)                tAAGCCGGGAGAGAACTTCACCGCAGCAGCGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTAAACGCAAAGACTTTATCCGTCTGGGCGCGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAACACTGCAGCCAACATCATCCGCGCGCTGGGCAAAATAATCGGCAACGGTCACCTGCACAAAAGCGAGAAGCCGGAACCCTGGTGCGTTGCCTGCCGATCTgcgc                                                                                                                                                                                                                     >  2:376468/1‑251 (MQ=255)                 aaGCCGGGAGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCAGCGCATACGCGGCGACCCAGGTTGACGGTCAACGCACAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCACCGAACACCTGCACAAAGGCGCGACGCAGGTACACTGGTGCGTAGACTGCCGATCTCCGCt                                                                                                                                                                                                                    >  1:295770/1‑251 (MQ=255)                  aGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGACTACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTCTCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTCCACTGGTGCGTTGACTGCCGTTCTGCGCTg                                                                                                                                                                                                                   >  1:28078/1‑251 (MQ=255)                    ccGGGTGAGAAATTCCCCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTCATCCGTCTGGGCGTGCTGGGCGACTGGTCGTACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCGGGGCAAAATCAGCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgc                                                                                                                                                                                                                 <  1:436177/251‑1 (MQ=255)                      gggTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgcgg                                                                                                                                                                                                               >  1:274240/1‑251 (MQ=255)                       ggTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgcgga                                                                                                                                                                                                              <  1:452373/251‑1 (MQ=255)                        gTGAGAAATTCACCGCCGCCGGGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGgcgga                                                                                                                                                                                                              >  2:332217/1‑250 (MQ=255)                                    ccgccgccgCGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGCCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTg                                                                                                                                                                                                   >  1:415624/1‑249 (MQ=255)                                      gccgccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTa                                                                                                                                                                                               <  1:15645/251‑1 (MQ=255)                                         gccgAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTAAACTGGTGCGTTGACTGCCGGTCTGCGCTGGCGGAAGCGGAAGTTGAGTAtt                                                                                                                                                                                             >  2:342290/1‑250 (MQ=255)                                           cgAGTTCCGCGCCAAGTGCCGCTAATACGCGGCGACCAAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGTGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGTGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTTAGTATTACg                                                                                                                                                                                          <  2:300669/251‑1 (MQ=255)                                               ttCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAGTCATCGGCAACGGTCACCTGCCCAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAACAGGAAGGTGAGTATTACGACa                                                                                                                                                                                       >  2:441602/1‑250 (MQ=255)                                                 ccGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGGCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCAGCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCAACCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACaaaa                                                                                                                                                                                    <  1:611352/251‑1 (MQ=255)                                                  cgcgCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCCACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTCCCTGACCATGGACGTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGCCAAAAc                                                                                                                                                                                   <  1:565166/251‑1 (MQ=255)                                                    cgcCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACtt                                                                                                                                                                                 >  1:594982/1‑251 (MQ=255)                                                     gcCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGAGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGAGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTtc                                                                                                                                                                                >  1:80787/1‑251 (MQ=255)                                                        aaGTGCCTCGAATACGCGGCGTCCCAGGTTGACGGTCAACGCAATCACGTTATCCGGCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTGCAAAACTGAAGCCAACATCATCCGCGCGCTTGGCAAAATCATCGGCAACGTTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTTCTTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAACTTGAGTATTGCGAccaaccctctcc                                                                                                                                                                             <  2:400016/251‑12 (MQ=255)                                                        aaGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGAGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTATcc                                                                                                                                                                             >  1:321327/1‑251 (MQ=255)                                                            gCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTcc                                                                                                                                                                         <  2:573201/251‑1 (MQ=255)                                                                            gagccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACGTCAAAACTGAAGCCAACATCATCCGCGCGCGGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGGCCATCGACGTTGCt‑tttc                                                                                                                                                        <  2:347936/248‑3 (MQ=255)                                                                               ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACACAGGAGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTTCGCTGGCGGAAGCGGACGTTGAGTATTACGCCACAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAgg                                                                                                                                                     >  1:629772/1‑251 (MQ=255)                                                                               ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAgg                                                                                                                                                     <  1:183824/251‑1 (MQ=255)                                                                               ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAgg                                                                                                                                                     >  1:573239/1‑251 (MQ=255)                                                                               ccAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAgg                                                                                                                                                     >  2:499036/1‑251 (MQ=255)                                                                                 aGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGcg                                                                                                                                                   >  2:179881/1‑250 (MQ=255)                                                                                   gtttaCGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGTAAGTTGAGTATTACTACAAAACTGCTCCGTCCATCGACGTTGCT‑TTTCAGGcggt                                                                                                                                                 <  2:373043/247‑4 (MQ=255)                                                                                   gTTGACGGTTAACGCAAAGACTTTATCCGGCTGGGCGTGCTGGGCGACTGGTCGCCCCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGcggt                                                                                                                                                 <  2:377374/251‑4 (MQ=255)                                                                                     tGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCg                                                                                                                                               >  1:560331/1‑251 (MQ=255)                                                                                     tGACGGTCAACGCAAAGACTTGATCCGTCGGGGTGTGCCGGGCGATTGGGTGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCACCCGCGCCCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACTCTGGTTCGTGGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGcggtt                                                                                                                                                <  2:22921/250‑5 (MQ=255)                                                                                      gACGGTCAACGCAAAGAATTTATCCGTCTGGGCGTGCTGGGCGACTGGTAGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTTCGTCCATCGACGTTGCT‑TTTCAGGCGGTCg                                                                                                                                               <  1:462136/250‑1 (MQ=255)                                                                                        cGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATc                                                                                                                                            <  1:524925/251‑1 (MQ=255)                                                                                         ggTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCa                                                                                                                                           <  1:29104/251‑1 (MQ=255)                                                                                          gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAg                                                                                                                                          <  1:17400/251‑1 (MQ=255)                                                                                          gTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGAACCCGTACCTGACCATGGACTTCAAAACTGAAGACAACATCATCAGCGCGCTGAGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGACGCGGCAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATc                                                                                                                                            >  1:621505/1‑249 (MQ=255)                                                                                                 cAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCAc                                                                                                                                   >  1:435012/1‑251 (MQ=255)                                                                                                  acaGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGGACAATGGTGCGTTGACTGCCGTTCTGCGCTTGCGGTAGAGGAAGTTGAGTATTACGACAACACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCATGATGccct                                                                                                                                  >  2:547934/3‑248 (MQ=255)                                                                                                  aaaGACTTTAACCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACt                                                                                                                                  <  2:218403/251‑1 (MQ=255)                                                                                                     gACTTTATCCGTCTGGGCGTGCGGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                               <  2:493253/251‑1 (MQ=255)                                                                                                       cTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGaa                                                                                                                               >  2:602138/1‑249 (MQ=255)                                                                                                         ttATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGc                                                                                                                            >  1:124441/1‑250 (MQ=255)                                                                                                           aTCCGTCTGGGCGTGCTTGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAAAATCATCCGCGCGCTGGGCAAAATCAGCGGCACCGGTCACCTGCAACAAGGCGAGAAGCCGGTACACTGGCCAGTTGACTGCCATTCTGCGCTGGCGGAAGCGGAAGGTGCATATTACGACAAAACTTCTCCGTACATCGCCGTTGCT‑TTTCAGGCGGCCGATCATGAtcccctgaccgcaa                                                                                                                          >  1:305255/1‑237 (MQ=255)                                                                                                             ccGTCGGGGCGTGATGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAAGCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAAt                                                                                                                       <  2:549789/251‑1 (MQ=255)                                                                                                                   gggCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCg                                                                                                                 >  1:154280/1‑251 (MQ=255)                                                                                                                   aggCGTGCTGGGCGCTTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCGGCACCAAGGCGCGACGCCGGTACCCTGGTGCGTTGACTGCAGCTCTGCGCTGGCGGAAGCGGAAGATGAGGATTACGACAAAACTCCTCCGGCCATCGCCGTTGCTGTTAC‑GGCGGTCGATCAGGATGCACTGAACGCAACATTTGCCg                                                                                                                 >  2:538515/2‑251 (MQ=255)                                                                                                                            gggCGACTGGTCGCACCCTTACCTGACCATGGACTTCAAAACTGAAGCCAACATCAGCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACTCTGGTGCGTTGGCTGCCGTTCTGCGCTGGCTGAAGCGGAAGTTGAGTATTACGACAAAACTTCGCCTTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCaa                                                                                                          <  1:50212/249‑1 (MQ=255)                                                                                                                                gACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTa                                                                                                     >  1:472645/1‑250 (MQ=255)                                                                                                                                           cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                         <  1:450226/251‑1 (MQ=255)                                                                                                                                           cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                         >  1:14345/1‑251 (MQ=255)                                                                                                                                           cccGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGACACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAACATTTGCCGTAAGCAACGTTAACGGCCCAAtct                                                                                         >  1:100799/1‑251 (MQ=255)                                                                                                                                             cGTACCGGACCATGGCCTTCAAAACTGAAGCCAACATCATCCGTGCGTTGGGCAAAATCATCGGCAACGGTCACCTGAACAAAGGCGCGAAGTCGGTACACTGGTTCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGGCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGGATGTACTGAAAGCAAAAGGTGCCGTAATCAACGTTAACGGCCCTATCTCg                                                                                       <  2:226849/251‑1 (MQ=255)                                                                                                                                                aCCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTg                                                                                    <  2:196043/251‑1 (MQ=255)                                                                                                                                                  cTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGt                                                                                  >  2:312986/1‑251 (MQ=255)                                                                                                                                                    gACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                 >  2:178605/1‑249 (MQ=255)                                                                                                                                                    gACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                 >  1:217460/1‑249 (MQ=255)                                                                                                                                                    gACCATGAACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGCCGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGtg                                                                                 >  1:467737/1‑249 (MQ=255)                                                                                                                                                       cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCt                                                                            >  2:470229/1‑251 (MQ=255)                                                                                                                                                       cATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCt                                                                            >  1:608555/1‑251 (MQ=255)                                                                                                                                                          ggACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCAGCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTgg                                                                          <  1:44085/250‑1 (MQ=255)                                                                                                                                                           gACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGac                                                                        <  2:457221/251‑1 (MQ=255)                                                                                                                                                                cAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGaccacta                                                                   <  2:507242/251‑3 (MQ=255)                                                                                                                                                                        aaGCCAATATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCCCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGGTAACGGCGCAATCTCGCTGTTG‑ATCTGGACCTCTACGCCGTgg                                                           <  2:93980/251‑1 (MQ=255)                                                                                                                                                                           ccAACATCATCCGAGCGCTGGGAAAAATCATCGGCAAAGGCCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGACAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGAG‑ATCTGGACCACTACGCCGTGGAct                                                        >  2:297040/1‑251 (MQ=255)                                                                                                                                                                             aaCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGActct                                                      >  1:488819/1‑251 (MQ=255)                                                                                                                                                                              aCATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTctg                                                     >  2:469255/1‑251 (MQ=255)                                                                                                                                                                               catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCCAAATTCGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGGG‑ATCTGGACCACTACGCCGTGGACTctgc                                                    >  2:248470/1‑251 (MQ=255)                                                                                                                                                                               catcatCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTctgc                                                    >  1:166474/1‑251 (MQ=255)                                                                                                                                                                                        cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACACAGGCGCGAAGCCGGAACACTGGTGCGTTGCCTGCCGTTCTGCGCTGACGGAAGCTGAAGTTAAGGATTACGACAAACCTTCTCCGTCCATCGACGGTGCT‑TTTCAGGCGGTCAATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCACCTTTACCGGCCCAATCTCACTG‑TAGATCTTGACCACTACGCCATgggttcacccttcagacc                                           >  1:305054/1‑235 (MQ=255)                                                                                                                                                                                        cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAAcc                                           >  2:140942/1‑251 (MQ=255)                                                                                                                                                                                        cgcgcTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGATCGATCAGGATGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAAcc                                           >  1:375086/1‑251 (MQ=255)                                                                                                                                                                                                gCAAAATCATCGTCAACGGTCACCTGCACAAATGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCGGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGGCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCGGCCTGCGAACCGCGCAAtc                                   <  2:415624/251‑1 (MQ=255)                                                                                                                                                                                                     atcatcGGCAACGGTCACCTGCACAAAGGCGCGCAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAACGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGCACCACGCAACCTCTAt                              >  2:462002/1‑251 (MQ=255)                                                                                                                                                                                                         tcGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGATGGCGGAAGCGGAAGATGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAACTATATTGc                           >  1:564774/1‑250 (MQ=255)                                                                                                                                                                                                          cGGCAACGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCAc                         >  2:481011/1‑251 (MQ=255)                                                                                                                                                                                                               aCGGTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAt                    >  1:554175/1‑251 (MQ=255)                                                                                                                                                                                                                cGGTCACCTGCACAAAGGCGCGAAGCCGGAACACTGGTGCGTTGACTGCAGTTCTGCGCTGGCGGCAGCGGAAGTTGCGTATTACGCCAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGCCGATCCGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGAtt                   >  1:572363/1‑251 (MQ=255)                                                                                                                                                                                                                 gggcgccTGCTCAAAGGCGTGAAGCCGGTACACTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGGACCACTACGCCGTGGTCTCTGCCTGCGAACCGCGCAATCGCTATTGCACCTGAttt                  <  2:615824/246‑1 (MQ=255)                                                                                                                                                                                                                  gTCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTc                 >  2:156490/1‑251 (MQ=255)                                                                                                                                                                                                                   tCACCTTCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACGGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCa                          <  2:463459/241‑1 (MQ=255)                                                                                                                                                                                                                   tCACCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCa                          >  1:463459/1‑241 (MQ=255)                                                                                                                                                                                                                     aCCTGCACAAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGCGTCTTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCgcc              >  1:307181/1‑249 (MQ=255)                                                                                                                                                                                                                       cggcccaacggCGCGAAGCCGGTGCTCTGGTGCGTTGACTTCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTGCTCCGTCCATCGACGTTGCT‑TGTCAGGCGGTCGATCAGTATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCTCTGGTG‑ATCTGTACCACTACGCCTTGGACTCTGCCTGCGAACCGCGCACTCTCTATTGCACCTGATGTCGACTa            <  2:82847/242‑1 (MQ=255)                                                                                                                                                                                                                           acaAAGGCGTGAGGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCCTCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCACTCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAAGCCCTATTGACCCAGATTTCGCCTATgcg        >  2:378150/1‑251 (MQ=255)                                                                                                                                                                                                                            caAAGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATgcgc       >  2:549816/1‑251 (MQ=255)                                                                                                                                                                                                                             aaaGGCGCGAAGTCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTGCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCGGCGAACCGCGGAATCTCTAGTGCTCTTGATTTCGACTATGCGCt      <  2:399481/251‑1 (MQ=255)                                                                                                                                                                                                                             aaaGGCGCGAAGCCGGTACACTGATGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCAATCGACGTTGCT‑TTTCAGGGGGTCGATCGGGCTGCATGGAAAGCAAAATTTACCGTAAGCACCGTTAACGGCCCAACCTCGATGGGG‑ATCCGGACCCCCACGAATGGGACTTTGCCTCCGAACCGCACAATCTCTATTGCACTTGATTTCGACTATgcact      >  1:140196/1‑248 (MQ=255)                                                                                                                                                                                                                               aGGCGCGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTTAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTgg    <  2:375086/251‑1 (MQ=255)                                                                                                                                                                                                                                 gcgcGAAGCCGGTACACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTCCGACAAAACTTATCCGTCCATCGACGTTGCT‑TTTCAGGCGGTCGATCAGGGTGCACTGAAAGCACAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTG‑ATCTGGACCACTACGCCGTGGACTCTGCCTGCGAACCGCGCAATCTCTATTGCACCTGATTTCGACTATGCGCTGGTg  >  1:405879/1‑251 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                CGAGCAAGAATACGGTAAGCCGGGTGAGAAATTCACCGCCGCCGAGTTCCGCGCCAAGTGCCGCGAATACGCGGCGACCCAGGTTGACGGTCAACGCAAAGACTTTATCCGTCTGGGCGTGCTGGGCGACTGGTCGCACCCGTACCTGACCATGGACTTCAAAACTGAAGCCAACATCATCCGCGCGCTGGGCAAAATCATCGGCAACGGTCACCTGCACAAAGGCGCGAAGCCAGTTCACTGGTGCGTTGACTGCCGTTCTGCGCTGGCGGAAGCGGAAGTTGAGTATTACGACAAAACTTCTCCGTCCATCGACGTTGCT‑TTCCAGGCAGTCGATCAGGATGCACTGAAAGCAAAATTTGCCGTAAGCAACGTTAACGGCCCAATCTCGCTGGTA‑ATCTGGACCACCACGCCGTGGACTCTGCCTGCCAACCGCGCAATCTCTATTGCACCAGATTTCGACTATGCGCTGGTG  >  NC_000913/22708‑23182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑