Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	22,373	T→C	intergenic (+25/‑18)	ribF → / → ileS	bifunctional riboflavin kinase/FMN adenylyltransferase/isoleucine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	22,373	0	T	C	100.0%	201.3 / NA	65	intergenic (+25/‑18)	ribF/ileS	bifunctional riboflavin kinase/FMN adenylyltransferase/isoleucine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/27);  total (38/27)

CAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTT  >  NC_000913/22128‑22600                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    cAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGa                                                                                                                                                                                                                                <  2:215071/251‑1 (MQ=255)                  ccAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAg                                                                                                                                                                                                               <  1:589695/251‑1 (MQ=255)                    aaCGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTg                                                                                                                                                                                                             >  2:492222/1‑251 (MQ=255)                          tGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACtat                                                                                                                                                                                                        <  1:586820/250‑1 (MQ=255)                              ggTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATc                                                                                                                                                                                                   >  2:324067/1‑251 (MQ=255)                              ggTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATc                                                                                                                                                                                                   >  2:290335/1‑251 (MQ=255)                                        agcagcTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAAt                                                                                                                                                                                         <  1:136620/251‑1 (MQ=255)                                        agcagcTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAAt                                                                                                                                                                                         <  2:426182/251‑1 (MQ=255)                                         gcagcTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAAtt                                                                                                                                                                                        >  1:367176/1‑251 (MQ=255)                                          cagcTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAAttt                                                                                                                                                                                       <  2:181441/251‑1 (MQ=255)                                             cTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGcc                                                                                                                                                                                    >  2:473978/1‑251 (MQ=255)                                                      aaTTTGTTAGATGTTGCAATGGTCCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAgg                                                                                                                                                                           <  1:15658/250‑1 (MQ=255)                                                       aTTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAggg                                                                                                                                                                          >  1:484470/1‑251 (MQ=255)                                                        tttGTTAGATGTTGCAATGGAACTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCCGATTGCGCGTGATGGATTAACCGCCCGAGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGCAACCCGGAACCGCGAATCTGATGAGTGACTATAAATCAACACTGAATTTGCCGGAAACAGGGt                                                                                                                                                                         >  2:366282/1‑251 (MQ=255)                                                          tGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACACGTAGTGCTGCGTAAAAAAATACGCCATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGCATCTGCTGAGTGACTATAATTCAACCCTGAATTTGCCGGAAACAGGGTTc                                                                                                                                                                       >  2:360234/1‑251 (MQ=255)                                                          tGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTc                                                                                                                                                                       >  1:606958/1‑251 (MQ=255)                                                           gTTAGATGTTGCAATGGATCTTTACGGTCTCCATATCCAAGTAGTGCTGGGTAAAAAAATACGCAATCAGCAGCGATTTGCGTCGCTGCACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGATCCGAGAATCTGATGAGTGACTATTAAGCAACCCTGAATTGGcctgtatcacggttc                                                                                                                                                                       <  2:582087/250‑14 (MQ=255)                                                           gTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTc                                                                                                                                                                       <  1:45342/250‑1 (MQ=255)                                                             tAGATGTTTCCATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGCTGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAGGCCTGTTCTGTAATCAAACCGAAACACGTAACCGAGAATCTGATGGGTGACTATAAATCAACCCTGCATTTGCCGGaaccatgattccc                                                                                                                                                                     >  2:20467/1‑239 (MQ=255)                                                             tAGATGATGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCg                                                                                                                                                                    >  1:468229/1‑251 (MQ=255)                                                                  gTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGGTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTTCCGGAAACAGGGTTCCCGATGc                                                                                                                                                                <  1:244053/250‑1 (MQ=255)                                                                                ttACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGcc                                                                                                                                                  >  1:259232/1‑250 (MQ=255)                                                                                     gTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAgcgc                                                                                                                                            >  1:240790/1‑251 (MQ=255)                                                                                             ataCAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAAccggg                                                                                                                                    <  2:485183/251‑4 (MQ=255)                                                                                             ataCAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAAccgg                                                                                                                                     <  1:473978/250‑3 (MQ=255)                                                                                             ataCAAGTAGGGCTGCGTAAAAAAATACGCAATGAGCAGCGATGTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCACCCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCCAGCGCGAAccgg                                                                                                                                     <  2:248262/250‑3 (MQ=255)                                                                                               aCAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGaa                                                                                                                                  <  2:260503/251‑1 (MQ=255)                                                                                                  agtagtGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGc                                                                                                                               <  1:290335/251‑1 (MQ=255)                                                                                                    gaatcctgcTTAAAAAAATACGCAGTAAGCAGCGATTTGCGTAGCTGGACGAAATGAAAGCGCAAAGTGCACGTGATGAAATAACCGCCCGCGAATTTGTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACAGGGAACCGAGAATCTGATGAGTGACTATAAATCATCCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCAGGAATGCTg                                                                                                                             <  2:286780/246‑1 (MQ=255)                                                                                                         ctgcGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGcgcg                                                                                                                        >  1:493253/1‑251 (MQ=255)                                                                                                                 aaaaaaACGCAATGAGCAGCGGTTTGCGCCGCTGAACGAACTGAAAGCGCAGATTGCGCGTGATGGATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGAGTTGGACtg                                                                                                                >  2:565166/1‑251 (MQ=255)                                                                                                                 aaaaaTACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtg                                                                                                                <  1:276564/251‑1 (MQ=255)                                                                                                                     aTACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatga                                                                                                            <  1:354637/251‑1 (MQ=255)                                                                                                                       aCGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAAACCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACtgatgatg                                                                                                          >  2:397569/1‑251 (MQ=255)                                                                                                                              gAGCAGCGATTTGCGTCGCTGGACGCACTGAAAGCGACGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTCTGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGAGCGAACCGGGACTGCTGGCGCGTTGGACTGCTGATGATCTGt                                                                                                    >  2:407826/1‑250 (MQ=255)                                                                                                                                         tgagTCGCTGGCCGAACTTAAAGCGCAGATTGCGCGTTATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCatcatc                                                                                         <  2:18065/247‑1 (MQ=255)                                                                                                                                              cgatGGACGAACTGAAAGCGCAGATTGCGCGAGATGAATTAACCGCCCGCGAATTTTTTAGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGACTTTGCCGAAAACAGGGTTCCCGATGCGTGTCTATCTCGCCACGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATTATCTTTACGGCATCATCCGTGCg                                                                                   >  2:462136/4‑251 (MQ=255)                                                                                                                                                 tGGACGAACTGTAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGACTTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGCGAATCTGATGAGTGCCTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCt                                                                                >  2:325732/1‑251 (MQ=255)                                                                                                                                                  ggACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGc                                                                                 <  1:39631/249‑1 (MQ=255)                                                                                                                                                   gACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTa                                                                               >  1:196088/1‑250 (MQ=255)                                                                                                                                                    aCGAACTGAAAGCGCTGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGTCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAAGCAACCCTGAATTTGCCGGAAATAGGGTTACCGATGCGGGGCGACCCCGCCAAGCGGGAACCGGGAACGCGGGCGCGTGGGACTGAGGATGATCTGTACGGCATCACTCGTGCGGCTaaa                                                                             <  2:614913/251‑1 (MQ=255)                                                                                                                                                           gAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGCATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGc                                                                       >  1:32051/1‑250 (MQ=255)                                                                                                                                                           gAAAGCGCAGATTGCGCGTGATGAATTAACAGCCCGCGAATTTTTTGGGCTAACAACACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCATGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGc                                                                       >  1:557811/1‑250 (MQ=255)                                                                                                                                                            aaaGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCa                                                                      >  1:112342/1‑250 (MQ=255)                                                                                                                                                             aaGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCaa                                                                     >  1:554911/1‑250 (MQ=255)                                                                                                                                                                cgcAGAGTGCTCGTTATGAATTAACCGCCCGCGAATTTGTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAACCACGTAACCGAGAATCTGATGAGGGACTATAAAGCAACCCTGAATATGCCGGAAACAGGGTTCCCGAAGCGTGGCGATCGCTCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGAGGATGATCGGAACGTCATGATCGGTGCGGCGAAAAAAGTCaaaaaa                                                                 <  2:625416/251‑1 (MQ=255)                                                                                                                                                                  cAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAAc                                                               >  1:93980/1‑251 (MQ=255)                                                                                                                                                                    gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCt                                                             >  1:597050/1‑251 (MQ=255)                                                                                                                                                                    gATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCt                                                             <  2:597050/251‑1 (MQ=255)                                                                                                                                                                       tGCGCGTGACGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGATTAAGCCTGTTATGTAATCAAACCGAAACACGGCACCGGGGATCTGATGAGTGACTATGAATCAACCCTGAATATGCCGGCACAAGGGTTCACGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGCACGCTGGCGCAATGGACTGATGATGATCAGTACGGCATCATCCGTGGGGATAACAAAGGCACAAACACCTTc                                                           >  1:611131/1‑250 (MQ=255)                                                                                                                                                                       ggcgcGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCTGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGAGCTCGCCAAGCGCGAACCGGGAATGCTGGCGCTTTGGACTGATGATGATCTGTACGGCATCAGCCGTGCGGCTAAGAAAGGCAAAAAAACCTTCa                                                          <  2:64003/250‑1 (MQ=255)                                                                                                                                                                         cgcgTGATGAATTAACCGCCCGCGACTTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCAtt                                                        >  2:35319/1‑251 (MQ=255)                                                                                                                                                                           ggTGATGAATTAACCGCCCGCGAATGTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCt                                                      <  2:521275/250‑1 (MQ=255)                                                                                                                                                                                 gaATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAA‑TCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCatgat                                               >  1:532381/1‑251 (MQ=255)                                                                                                                                                                                    ttAACCGCCCGCGAATTTTTGGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGCAACACGGAACCGAGACTCTGATGAGTGACTATAAATCAACACTGAATTTGACGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGCCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATgg                                             >  2:29104/1‑251 (MQ=255)                                                                                                                                                                                         cgcccgcGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCACTCTGCATGATGGCcctc                                        >  1:549789/1‑251 (MQ=255)                                                                                                                                                                                            ccgcGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCtt                                     >  1:457221/1‑251 (MQ=255)                                                                                                                                                                                                            cTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCAAATGGCAGCAt                     >  2:306190/1‑251 (MQ=255)                                                                                                                                                                                                             tAACAAAGCCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAACTCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGGACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCAtt                    >  1:445425/1‑251 (MQ=255)                                                                                                                                                                                                                   aaCCGGCTTAAGCCTGTTATGTAATCAAACCTAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCTAATGGCAGCATTCATAtt              <  1:537890/251‑1 (MQ=255)                                                                                                                                                                                                                    aCCGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTg             >  1:347936/1‑251 (MQ=255)                                                                                                                                                                                                                      cGGCTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGAGGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGt           <  1:365701/251‑1 (MQ=255)                                                                                                                                                                                                                         cTTAAGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCAc        >  1:218403/1‑251 (MQ=255)                                                                                                                                                                                                                            aaGCCTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCCTTCATATTGGTCACTCg     >  2:489866/1‑251 (MQ=255)                                                                                                                                                                                                                                cTGTTATGTAATCAAACCGAAACACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCGGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGtt  >  2:202824/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                    CAAACATCGGAACACGCCCAACGGTTGCCGGTATTCGCCAGCAGCTGGAAGTGCATTTGTTAGATGTTGCAATGGACCTTTACGGTCGCCATATACAAGTAGTGCTGCGTAAAAAAATACGCAATGAGCAGCGATTTGCGTCGCTGGACGAACTGAAAGCGCAGATTGCGCGTGATGAATTAACCGCCCGCGAATTTTTTGGGCTAACAAAACCGGCTTAAGCCTGTTATGTAATCAAACCGAAATACGGAACCGAGAATCTGATGAGTGACTATAAATCAACCCTGAATTTGCCGGAAACAGGGTTCCCGATGCGTGGCGATCTCGCCAAGCGCGAACCCGGAATGCTGGCGCGTTGGACTGATGATGATCTGTACGGCATCATCCGTGCGGCTAAAAAAGGCAAAAAAACCTTCATTCTGCATGATGGCCCTCCTTATGCGAATGGCAGCATTCATATTGGTCACTCGGTT  >  NC_000913/22128‑22600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑