| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,038 | C→T | L568L (CTG→TTG) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,038 | 0 | C | T | 98.7% | 231.0 / NA | 78 | L568L (CTG→TTG) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (36/41); minor base G (0/1); total (36/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
CGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTT > NC_000913/1792‑2276 | cGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTgcg > 1:79220/1‑251 (MQ=255) acAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGa > 2:66435/2‑249 (MQ=255) aGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GGGGATCAATATGCCGACTTCTTGCGCGAAGGttt < 1:518264/251‑1 (MQ=255) ggctgAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGACTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGCTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGCCTTCTTGCGAGAAGGtttaccc > 1:357289/1‑247 (MQ=255) aTAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACg < 1:54758/251‑1 (MQ=255) aaaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGtt < 1:604093/235‑1 (MQ=255) aaaCATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGtt > 2:604093/1‑235 (MQ=255) aaaCATATCGACGTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGcc < 1:177906/251‑1 (MQ=255) cATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGaa < 1:495710/251‑1 (MQ=255) cATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGaa < 1:512921/251‑1 (MQ=255) atCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTGCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACa < 2:597916/250‑1 (MQ=255) tCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGCCTTCTTGCGCGAAGGTTTCCACGTTTGCACGCCGCACaa > 2:572217/1‑250 (MQ=255) acgggtCTGCGGTGTGGCCAACTCGAAGGCCCTGCTCACCAAGGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTGTAATCTCGGGCGCTTAATTTGCCTCGTGAAAGAATATCATCTGCTGAACCCGGGCATTGTTGACTGCACTTCCAGCCAGGCAGTT‑GCGGATCAATATGCCGACTTCTGGCGCGAAGGTTTCCACGGTGTCGCGCCTAACAAAAAGGCCa < 2:30811/247‑1 (MQ=255) cGTGTCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGACCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGACGAACCAAAAGGCCa > 2:375691/1‑250 (MQ=255) gtCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAaca < 2:527310/250‑1 (MQ=255) tCTGCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGCATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAacaac > 1:610390/1‑249 (MQ=255) gCGGTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGGG‑GCGGACAAATATGCCGACATCTTTCGCGCAGGTTTTCACGTTGTCACGCCGAACAAAAAAGACAacacac > 2:517883/1‑247 (MQ=255) ggTGTTGCCAACTCGAAGGCACTGCTCCCCAAGGTGCATGGCCTAAAGCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCGCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCGGCTGAACCCGGTCAGTGTGGACTGCACTTCCAGCCAGTCAGTG‑GCGGATCAATATGCCGACGTCTGGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACgtcgtc < 2:256829/251‑1 (MQ=255) ggTGTTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCtcgtc > 1:201273/1‑251 (MQ=255) gtTGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGAGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGAt < 1:154687/251‑1 (MQ=255) ttGCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACCCCGAACAAAAAAGCCAACACCTCGTCGATg > 1:438327/1‑251 (MQ=255) gCCAACTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGa < 1:166784/251‑1 (MQ=255) aaCTCGAAGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAATGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATta < 1:364096/251‑1 (MQ=255) cGAAGGCACTGCTCACCTATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATtactac < 1:448544/251‑1 (MQ=255) gAAGGCACTGCTCATCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTGCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTAcc < 2:512940/251‑1 (MQ=255) aGGCACTGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCAt < 1:537327/251‑1 (MQ=255) gcactGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATct > 1:306512/4‑250 (MQ=255) cactGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGCTGGATTACTACCATctt > 2:473525/3‑249 (MQ=255) tGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTg > 1:309423/1‑251 (MQ=255) tGCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTAATCCCATCTGTTg > 1:327438/1‑251 (MQ=255) gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTg < 1:191830/250‑1 (MQ=255) gCTCACCAATGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACGTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGtt < 1:370354/249‑1 (MQ=255) ccaatggggacggCCTAAATCTGGAAAACTGGCAGGAAGAACTGTCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCCGGCAGTG‑GCGGCTCCATCTGCCGTCTTCTTTGGCGAAGGATTCCCCGTTGTCGCGCCGCATAAAAAAGCCAACACCTCGTCGATGGATTACTAccctcttttgcgtcat > 2:169551/12‑237 (MQ=255) aaTGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAATCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCTCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATgc < 2:485518/251‑1 (MQ=255) aaTGTGCATGGCCTAAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTGCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATgc < 1:306942/251‑1 (MQ=255) cctaaaTCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAAt < 1:488778/247‑1 (MQ=255) ggtGGAAAACTGGCAGGAAGACCTGGCCCAGGCCAAAGAGCCGTTTAATGTCGGGCGCTTAATTCGCGTCGTGAAAGAATATCATCTGCTGGACCCGGTCATTGTTGACTGCACTTCCAGACAGGCAGTG‑GCGGAGCAATATGCCGACTTCGTGCGCGTAGGTTTCCACGTTGGCGCTCTGAGCAAAAAGGCCAACACCTCGTCGATGGATTACTACCAGCTGTTGCGTCATGCGGCGGAATAATCgcggt < 2:206870/249‑2 (MQ=255) cTGGCAGGAAGAACTGGCCCAAGCCAAAGAGCCGTTTAATATCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCCCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGAGGGCGAAAAATCGGGGCGTAAATTcc > 2:398032/1‑251 (MQ=255) gCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCctct > 1:385052/1‑251 (MQ=255) aGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCCTCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTAt > 2:107739/1‑251 (MQ=255) tGGCGCACGCCACAGAGCCGTTTCATCTCGGGCGCTTCATTCGCCTCGTGAAAGAATATCATCTGCTGCACCCGGTCATTGTTGACTGCCCTTCCAGCCAGGCAGTG‑GCGGATCAATCTGCCGACTTCTTGCCCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCCCGTCGCTGGATTACTACCATCTGTTGCTGAATTGGGCGGAAAAATCACGGCGTACATTACTCTATGCCAC‑Caa > 2:607404/1‑250 (MQ=255) ggCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACg < 2:128922/251‑1 (MQ=255) ggCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCGATGACAC‑CAAc < 2:79220/250‑1 (MQ=255) gcAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTg < 1:274815/251‑1 (MQ=255) cAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTgg < 1:76037/251‑1 (MQ=255) aaGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGa < 1:454678/251‑1 (MQ=255) tttAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTTGATTACCGGt > 2:374643/1‑251 (MQ=255) aaTTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCaa > 1:471191/1‑250 (MQ=255) ccTCGTGGAAGAATATCATCTGCTGAACCCGGTCATTGTTGCCTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CCACGTTGGGGCTGGATTACCGGTTTTTGGGAACCTGCAAAATCTGc > 2:360149/1‑251 (MQ=255) cGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGGCGAATTCTTGCGCGAAGGTTTCCAAGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGCTTACTAACCTCTGTTGCGTCCTGCGGCGGGAAAATCGCGGCGTAAATTCCTCTCTGACAC‑CAACGTTGGGGCTGGGTTACCGGTTCTTGAAAACCTGCAAAATCTggtcc > 1:350956/1‑247 (MQ=255) aaTATCATCTGCTGAACCCGGTCATTGTTGCCTGCACTTCCAGCCCAGCAGTG‑GCGGATCTATATGTCGACTTCTAGCGCGAAGGGTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCCGAAAAATCGCGGTGTAAATTCATTCATGACAC‑CAAAGATGGGGCTGGATTAACGGGTATTGAGAACCTGCAAAATATGCTCAATGctggg > 2:105358/1‑247 (MQ=255) atatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtg < 1:444457/251‑1 (MQ=255) tatCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGGAAATTCCTCTATGCCAC‑CAACGTTGGGGCTGGATTAACGGCTATTGAGGACCTGCAAAATATGCttcatgccggtgg > 2:267696/1‑239 (MQ=255) atcatcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgat < 1:57667/251‑1 (MQ=255) catcTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGtgatga < 1:77990/251‑1 (MQ=255) atcTGCTGTACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCCGTG‑GCGGATCAATATGACGACTTCTTGCGCGAAGGTTTCCCCGTTGTCACCCCGAACAAAAAGGGCAAAACCTCGTCGATGGATTACTACCATCTGTTGCGGCAAGCAGCGGAAAAATTGCGGCGTAAAGTCATCTATGAAACACAA‑GATGGGGCTGGATTACCGGTTATTGAGAAACTGCAGCATCTGCTCAAtacgggggatgg > 2:545917/1‑239 (MQ=255) gctgAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTAGGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATtg < 1:314137/250‑1 (MQ=255) gAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGACCCTGCAAAATCTGCTCAATGCTGGTGATGAATtgatg < 1:100849/250‑1 (MQ=255) aCCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAg < 1:473525/251‑1 (MQ=255) cccGGTCATTGTTGACTGCACTTCCAGCGAGGCA‑TGTGCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAATGCCAACACCTCGTCGATGGATTACTACCGGCGGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGGTGGGGCTGGATTACCGGGGAGGGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGt < 1:228698/251‑1 (MQ=255) acggccATTGTTGACTGAACTTCCAGCCAGGCAGTG‑GCGGACCCATATGGCGACTTCTTGCGCGAAGGTTTCCACCTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCATCATGCGGCGGAAAACTCGCGGGGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGACAAATCTGCTCAATGCTGGTGATGACTTGCAGAAGtt > 2:528503/6‑251 (MQ=255) gTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACGTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCTGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAGCCTGCAAAATCGGCTCAATGCTGGTTATGAATTGATGGAGTtctc < 2:36198/251‑1 (MQ=255) cATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTcc < 1:572217/250‑1 (MQ=255) aTTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGTATTGATGAAGTTCTCCgg > 2:521732/1‑251 (MQ=255) ggtttaCTGCACTTCCATCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCGCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGTATTACCGGTGATTGAGAACCTGCAAAATCTGCTCAATGCTGGTTATTAATGGATGAAGTtcggcggt < 2:30299/245‑7 (MQ=255) ttgACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAtt > 2:160704/1‑251 (MQ=255) tgACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGGGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAAAGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGAAttc > 1:535943/1‑251 (MQ=255) aCTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCCCGGTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CCACGTTGGGGCTGGATTACCGGTTCTTGAGAACCTGCAAAATCTGCTCAATGCTGGGGATGAATTGATGAAGTTCTCCGGCAttctt > 2:129073/1‑251 (MQ=255) aCTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctt > 1:197974/1‑251 (MQ=255) tGCACTTCCAGCCAGGCAGTG‑GCGGATTAATATGCCGACTTCTTGCGCGAAGGTTTCCACGGTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGGTTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGGTTACCGGTTATTGGGAACCTGCAAAATCTGCTCAATGATGGTGATGAATTGATGAAGTTCTCCGGCAttctttc > 2:390980/1‑251 (MQ=255) gCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCAttctttca < 1:284524/251‑2 (MQ=255) cTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCCACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAAAACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTACTCTATGACAC‑CAAAGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAACATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGt > 1:118569/1‑251 (MQ=255) ccagGCAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCTTCGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCTTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGTGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCttt < 1:278152/251‑1 (MQ=255) cAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGGAGGGTTCCACGGTGTCCCGCCGAACAAAAAAGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGa > 2:61587/1‑142 (MQ=255) cAGTG‑GCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGa < 1:61587/142‑1 (MQ=255) g‑gCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGACGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCt > 1:89774/1‑251 (MQ=255) g‑gCGGATCAATATGCCGACTTCTTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCt < 1:374643/251‑1 (MQ=255) atGCCGACTTCTTGCGCGAATGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCTGTTGCGTCATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGTGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTTCTCAATGCTGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCAGGTTCGCTTTCTTATATCTTCGGCAAGtt < 1:251784/250‑1 (MQ=255) | CGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCTGCGGTGTTGCCAACTCGAAGGCTCTGCTCACCAATGTACATGGCCTTAATCTGGAAAACTGGCAGGAAGAACTGGCGCAAGCCAAAGAGCCGTTTAATCTCGGGCGCTTAATTCGCCTCGTGAAAGAATATCATCTGCTGAACCCGGTCATTGTTGACTGCACTTCCAGCCAGGCAGTG‑GCGGATCAATATGCCGACTTCCTGCGCGAAGGTTTCCACGTTGTCACGCCGAACAAAAAGGCCAACACCTCGTCGATGGATTACTACCATCAGTTGCGTTATGCGGCGGAAAAATCGCGGCGTAAATTCCTCTATGACAC‑CAACGTTGGGGCTGGATTACCGGTTATTGAGAACCTGCAAAATCTGCTCAATGCAGGTGATGAATTGATGAAGTTCTCCGGCATTCTTTCTGGTTCGCTTTCTTATATCTTCGGCAAGTT > NC_000913/1792‑2276 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |