| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 21,617 | A→C | R71R (AGA→CGG) | ribF → | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 21,617 | 0 | A | C | 98.8% | 240.6 / NA | 80 | R71R (AGA→CGG) | ribF | bifunctional riboflavin kinase/FMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (42/37); total (43/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GATTTTCACTGTTTTGAGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCG > NC_000913/21383‑21854 | gATTTTCACTGTTTTGAGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCg > 1:529638/1‑251 (MQ=255) aTTTTCACTGTTTTGAGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCgg > 2:228554/1‑251 (MQ=255) tttCACTGTTTTGAGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCggg > 2:550006/1‑250 (MQ=255) aCTGTTATGATCCAGACATGAAGCTGATACGCGGCCTACATACTCTCAGCCAGGCCCCGCA‑CGAAGGGTGTGTGCCGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGCAGACGGGCGCAATCGCAACTTACCGGTGC‑TGGTGATGCTTTTTGAACCTCACCCACTGGAACTCTTTGCTACCGATAAAGCCACGGCCCGGCTGACCCGGcaccgggacaa > 2:254172/1‑240 (MQ=255) tttGAGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGCCGCCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCg > 1:365422/1‑251 (MQ=255) aGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTAc < 2:505693/251‑1 (MQ=255) aaGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAgtg > 2:523873/1‑251 (MQ=255) aGCTGATACGCGGCATACATACTCTCAGCCACGCCCCGCA‑AGACGGGCGCGTGCTGACTATTGGTAATTTCGACGGCGTGCATAGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCACCTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCACCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTCCCTTGCCGAgtgg > 1:525842/1‑250 (MQ=255) tGATACGCGGCATACATATGCTCAGCCAGTCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGGAATGTCGACGGCGTGCATCGCGGTCCTCGCGCGCTGTTACAGGTCTTGCATGAAGAAGGGCGCAAGCGCAACTTTCCGGTGA‑TGGGGATGCGTGGTGAACCGCAACCGCTGGAACTGTTTGCAACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGGGACCTGGCAGAGtgtg < 2:625991/249‑1 (MQ=255) tGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGC‑TGGTGATGCTTTTTGCATCTCAACCACTGGAACTGTTTGCTACCGCTAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGtgtg > 1:625991/1‑249 (MQ=255) aTACGCGGCATACCTAATCTCAGCCAGGCCTCGCA‑AGAAGGGTGTGTGCTGGCTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTCCCATGCAGAGTGTGGCGt < 2:350668/251‑1 (MQ=255) cgcgGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGa < 2:627883/251‑1 (MQ=255) atacatcATCTCAGCCAGGCCCCGCA‑AGACGGGTGTGTGCTGACTATTGGTCATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACgg > 2:416804/1‑250 (MQ=255) acataATCGCAGCCAGGCCACGAACGGAAGGG‑GGGTGCTGACTATTGATAATTTCGACGGCGTGCAGAGCGGTCATCGCGCGCTGTTACAGGGATAGCAGGAAGAAGGGCGCAAGCGCAACTTCCCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACACGGCTGCGGGAAACACTGCGTTACCTTGCAGAGGGGGGCGTTGATTACGTg > 2:540151/1‑250 (MQ=255) cataATCTCAGCCTGGCACCGCA‑AGATGGGTGTGTGCTGACTAGTGGTAATTGCGACGGCTTGCATCGCGGCCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTAACGGTGA‑TGTTGATGCTTTTTGAACCTCAACCATTGGAACTTTTTGCTACCAATAAAGCCACGGCACGGCTGACCCGGCTGCGTAAAACACTGCGTTACCTTGCGGAGTGTGGCGTTGATTACGTGCt < 1:214665/251‑1 (MQ=255) cataATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCt < 2:70939/251‑1 (MQ=255) ccAGCCATGCCCCGCA‑AGAAGGGTGTTGGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCAGCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCATGCTCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTTCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCTTTGATTACGTGCTgtgcgtt < 2:378376/250‑2 (MQ=255) cAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTgtgcgtgc < 1:431343/251‑1 (MQ=255) cAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTgtgcgt < 2:243981/249‑1 (MQ=255) cAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTgtgcgtgc < 2:3413/251‑1 (MQ=255) aGCCAGGCCCCGAA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTCCGTGCTGtgcgtgcg > 2:490240/1‑251 (MQ=255) ccAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGGGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCATAGTGTGGCGTTGATTACGTGCTGTGCGTGCGtt < 2:518426/251‑1 (MQ=255) ggCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCg > 1:24440/1‑251 (MQ=255) cGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAgg < 1:623833/251‑1 (MQ=255) gggTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGGTTCGACAGGCGTTTcgc < 1:430784/251‑1 (MQ=255) agtgtgCTGCCTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAGGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGAGCGTGCGTTTAGACAGGCGTTTCgcggc > 2:582260/2‑251 (MQ=255) gtgtgCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCgcggcg > 1:133570/1‑251 (MQ=255) gtgCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGtt < 1:48508/251‑1 (MQ=255) gtgCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGt < 1:360800/250‑1 (MQ=255) tgCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAGGCCACGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGAGCGTGCGTTTCGACAGGCGTTTCGCGGCGtt > 1:89633/1‑250 (MQ=255) aTTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCa < 1:434669/251‑1 (MQ=255) tGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCaa < 2:14633/250‑1 (MQ=255) aTTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTtc < 1:156420/251‑1 (MQ=255) tttCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCtgt > 2:109986/1‑203 (MQ=255) tttCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCtgt < 1:109986/203‑1 (MQ=255) ttCGACGGCGGGCATCGCCGTCCTCGCGCGCTGTTACAGTGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACTTCAACCACTGGAACTGTTTGCTACCTATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAATACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCTTGCGTTTCGACATGCTTTTCGCGGCGTTAACCGCGCAAAATTtca < 1:128133/250‑1 (MQ=255) gcgGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTg > 1:480377/1‑251 (MQ=255) aTCGCGCGCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAACATTTCATCAGCGATCTTCTGGTGAAGctt > 2:472167/1‑249 (MQ=255) cgcgcgCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGcttt < 2:24440/250‑4 (MQ=255) cgcgcgCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCtttt < 2:529638/251‑1 (MQ=255) cgcgcgCTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCtttt > 1:481855/1‑251 (MQ=255) agcgcgcTGATACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGCGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGGGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTACATCAGCGATCTTCTggggaggctttt > 2:483275/2‑241 (MQ=255) cgcgcTGTTACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTgc < 2:137119/251‑1 (MQ=255) gTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCACCCACTGGCACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCCTCAGCGATCTACTGGTGAAGCTTTTGCGCGTa > 2:250817/1‑250 (MQ=255) tACAGGGATTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGGGCGTTTCGCCAGGCGTTTCGCGGCGTTAACCGCGCAAACTTTCATCAGCGATCTTCTGGTGAGGCTTTTGCGCGTaaat > 2:603366/1‑250 (MQ=255) cAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGt > 1:50896/1‑251 (MQ=255) aGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACAGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCga > 2:530332/1‑249 (MQ=255) aagaagGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAACTTTCTTGCCGTAGGt > 2:455309/1‑251 (MQ=255) agaagGGCTCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTGTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGtg < 2:534849/251‑1 (MQ=255) agaagGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGtg > 1:72649/1‑251 (MQ=255) gcgcaagcgcaaCGTACATGTTA‑TGGTTATGCTTTTTGAACCTCAACCACTGGATCTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGTCTGCGTGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGTGGCGTTAACCGCTCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGAtt < 1:630308/251‑1 (MQ=255) cgcaagcacaaCGTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACGGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGGGAAGCTTGTGCGCGTCAAATTTCTTGCCGTAGGTGATGAttt < 2:557790/251‑1 (MQ=255) gcaagcgcaaCTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTc > 1:501782/1‑251 (MQ=255) gcaagcgaaaCTTACCGG‑GACTAGTGAAGCTTTTTGCACCTCAAACACTGGAACAGCTTGCTACCGATAAAGCCCAGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGAGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGCAGCTTTTGCGCGTCAAATTTCTTGCCGCAGGTGATGATTTc > 2:390417/1‑251 (MQ=255) caagcgcaaCTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTcc > 1:248208/1‑251 (MQ=255) gcaaCTTACCGGGGA‑TTGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACAGATAAAGCCCCGGAACGGATGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGACGTTTAGCGGCGTTAACCGGGAAAAAATTCATCAGCGATCTTCTGTTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGGGATGTTTTCCGcctt > 2:601650/1‑248 (MQ=255) caaCTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTcc > 2:571254/1‑245 (MQ=255) caaCTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTcc < 1:571254/245‑1 (MQ=255) aaCTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTgg < 1:416804/251‑1 (MQ=255) gtga‑tggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGCTCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGCTGATTTCCGCTTTGGCGCTGGTCg > 1:266652/1‑251 (MQ=255) ga‑tggtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTg < 1:279781/251‑1 (MQ=255) tggtgatgCTTTTTGCACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGACACGGCTGACCCGGCTGCGGGAACAACTGCGTTACCTTGCAGAGTGTGGCGTTGCTTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGa > 2:43668/1‑250 (MQ=255) gtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGCGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTCGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAgg > 2:357387/1‑251 (MQ=255) gtgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCACGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGGGAAgg > 2:485961/1‑251 (MQ=255) gtgatgCTTGTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTTCCTTGCAGAGTGTGGCGTTGATTCCGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGAGCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATGTCCGCTTTGGCGCTGGTCGTGAAgg < 2:578927/251‑1 (MQ=255) tgatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTAACGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGCTACCGTGCAGAGTGTGGCGTTGCTTACGTGCTGTGCGTGCGTTTCGTCAGGCGTTTCGCGGCGTTAACCGCGCAAATTTTCATCAGCGATCTTCTGGTGACGCTTTTGCGCGTAAAATTTCTTGCCGTGGGTGATGATTTCAGCTTTGGCGCTGGTCGTGTAGGc > 1:286780/1‑251 (MQ=255) tgatgCGTTTTGAAAATCAACCACTGGAAATGTTTGCTACCGATAAAGCCAAGGCAAGGCTGACCCGGATGCGGGAAAAACTGCGTTACCTTGCAGAGAGTGGCGTTGATTAAGTGCTGTGCGTGCGTTTCGCCAGGCGTTTCGCTGCGTTAACCGCGCCAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTACAATTTCTGTCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAggg > 2:515485/1‑250 (MQ=255) gatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCg > 2:501254/1‑251 (MQ=255) gatgCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGAGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCg > 2:208971/1‑251 (MQ=255) ttGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCtt < 1:61033/250‑1 (MQ=255) aCCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGttat < 1:158424/251‑1 (MQ=255) aCCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTAc > 2:117932/1‑248 (MQ=255) aCTGGAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAg < 1:490240/251‑1 (MQ=255) ggAACTGTTTGCTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTg < 1:523873/251‑1 (MQ=255) aCTGTTTACTACCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTAATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGc > 2:414446/1‑250 (MQ=255) cTGTTTGCTACCGATAAAGCACCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCAt > 2:288670/1‑251 (MQ=255) aCCGATAAAGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTGCATCAGCGATCTTCTGGTGAAGCTTTTGCGTGGAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATAc < 1:295480/249‑1 (MQ=255) ccGATAAAGCCCCGGCACGGCTGCCCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGTCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGc < 1:305555/251‑1 (MQ=255) aaaGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTTTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAATGCGATTTCTTGTTATTACAGAAAGCGGGCATGGAATACGGCTTCg < 1:221999/250‑1 (MQ=255) aaaGCCCCGGCACGGCTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCTTTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCg > 1:524938/1‑250 (MQ=255) | GATTTTCACTGTTTTGAGCCAGACATGAAGCTGATACGCGGCATACATAATCTCAGCCAGGCCCCGCA‑AGAAGGGTGTGTGCTGACTATTGGTAATTTCGACGGCGTGCATCGCGGTCATCGCGCGCTGTTACAGGGCTTGCAGGAAGAAGGGCGCAAGCGCAACTTACCGGTGA‑TGGTGATGCTTTTTGAACCTCAACCACTGGAACTGTTTGCTACCGATAAAGCCCCGGCAAGACTGACCCGGCTGCGGGAAAAACTGCGTTACCTTGCAGAGTGTGGCGTTGATTACGTGCTGTGCGTGCGTTTCGACAGGCGTTTCGCGGCGTTAACCGCGCAAAATTTCATCAGCGATCTTCTGGTGAAGCATTTGCGCGTAAAATTTCTTGCCGTAGGTGATGATTTCCGCTTTGGCGCTGGTCGTGAAGGCGATTTCTTGTTATTACAGAAAGCTGGCATGGAATACGGCTTCG > NC_000913/21383‑21854 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |