Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,715	G→A	intergenic (+95/+96)	nhaR → / ← insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,715	0	G	A	100.0%	97.1 / NA	32	intergenic (+95/+96)	nhaR/insB1	DNA‑binding transcriptional activator NhaR/IS1 family protein InsB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (19/13);  total (19/13)

CCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19472‑19819                                                                                                                                                                                                                                                      |                                                                                                         ccGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGAGAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTTAGACATTATTTCCa                                                                                                    <  1:59559/251‑1 (MQ=255)      aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGt                                                                                               >  1:452022/1‑251 (MQ=255)        aaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                             >  2:504112/1‑251 (MQ=255)                       tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCACGCTTGAGACATTATTTCAATATGTACGCGGGCGaataaat                                                                               >  2:66654/1‑250 (MQ=255)                           cgcgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAgg                                                                          >  1:440411/1‑251 (MQ=255)                                cGAGAATGTGATGGAAGAGTATCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGTAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGTCATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCt                                                                     <  2:514076/251‑1 (MQ=255)                                      tgtgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTAc                                                               >  2:139363/1‑251 (MQ=255)                                         gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGATGAATCTGATTActtc                                                            <  1:602098/251‑1 (MQ=255)                                         gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGGTGTACATAACCA‑CGATTTTGACTCGGCGCAGTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGATGAATCGGATTActtc                                                            <  2:309447/251‑1 (MQ=255)                                            ggAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTActtcctt                                                         >  2:388775/1‑251 (MQ=255)                                               agagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCCATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAGGGTGGGGGGGATAGATTAGTTGTACAA‑ACCAACGATTTGGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCTTTTGTACGGGGGCGAATAAAAGGAGGAATTGGATAACTTCCTTCa                                                       >  1:443696/1‑250 (MQ=255)                                                      cATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATg                                               <  1:420005/251‑1 (MQ=255)                                                         gCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg                                            <  2:366629/251‑1 (MQ=255)                                                         gCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCCGCAATACGGAGGATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGAGTAGTTGTAAATAACCA‑CGATGTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg                                            <  1:74624/251‑1 (MQ=255)                                                          cTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAACGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg                                            >  1:189018/1‑250 (MQ=255)                                                               tttGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTgcaa                                         >  2:117305/1‑245 (MQ=255)                                                                ttacTGAGCGGATGAATCAGCACCCGGCGGTACAGCGATTCTGCAATACGGATTATTATGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGGGATAGATTAGGTGTACATAACCC‑CGGTTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATTGACGCGGGCGAATAAATAGAGGACTCTTATTACTTCCTTCATGGGGATGCTGAAAag                                       >  2:338612/4‑249 (MQ=255)                                                                ttGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGTTGCTGAAAag                                       >  2:494278/1‑249 (MQ=255)                                                                 tGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGTATGCTGAAAagag                                     <  1:120639/250‑1 (MQ=255)                                                                  gCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATAAGGCTTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATCACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGCTGCTGAAAAGagt                                    >  1:254604/1‑250 (MQ=255)                                                                   cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTCGTCAAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGagt                                    >  1:357536/1‑249 (MQ=255)                                                                        cGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTCTTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGaataaa                                                                                >  1:388183/1‑200 (MQ=255)                                                                        cGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTGGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGTGCGaataaa                                                                                <  2:388183/200‑1 (MQ=255)                                                                          gatgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTACGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa                               >  2:597083/1‑247 (MQ=255)                                                                          gatgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa                               <  1:597083/247‑1 (MQ=255)                                                                           attatTCAGCACCCGGAGGTACAGCGAATCTGCAATACGGATTAGTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGTCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCt                          <  2:347940/248‑1 (MQ=255)                                                                              atTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt                       >  1:369688/1‑248 (MQ=255)                                                                                    cACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccata                 <  1:62276/251‑10 (MQ=255)                                                                                                  gCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcg   >  1:609315/1‑228 (MQ=255)                                                                                                    gAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCCCCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATAACAA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATCAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcgt  >  1:569871/1‑226 (MQ=255)                                                                                                                                                          acctcccccc‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                             >  1:307949/6‑104 (MQ=255)                                                                                                                                                          acctcccccc‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                             <  2:307949/99‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                         CCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCCC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19472‑19819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑