| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 19,670 | T→A | intergenic (+50/+141) | nhaR → / ← insB1 | DNA‑binding transcriptional activator NhaR/IS1 family protein InsB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 19,670 | 0 | T | A | 97.3% | 108.8 / NA | 38 | intergenic (+50/+141) | nhaR/insB1 | DNA‑binding transcriptional activator NhaR/IS1 family protein InsB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (18/18); minor base C (1/0); total (20/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
CAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCC‑C‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA > NC_000913/19430‑19819 | cAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTATTTGTACATAACCA‑CGAtt < 2:488411/251‑1 (MQ=255) ccccAACGCTTTATGCATATGCCTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGAGGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGTGTGAGATAGATGAGTTGTACATACCCA‑CGATTTTGACTCGGCt < 2:416532/250‑1 (MQ=255) cccAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCa < 1:238209/251‑1 (MQ=255) ttATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTg < 2:312580/249‑1 (MQ=255) taCTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATGTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCTGCACCTCCCCGC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTGCATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTgaga < 1:591807/249‑1 (MQ=255) aCTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGAc > 1:342446/1‑250 (MQ=255) ccGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGAGAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTTAGACATTATTTCCa < 1:59559/251‑1 (MQ=255) aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGt > 1:452022/1‑251 (MQ=255) aaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc > 2:504112/1‑251 (MQ=255) tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCACGCTTGAGACATTATTTCAATATGTACGCGGGCGaataaat > 2:66654/1‑250 (MQ=255) cgcgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAgg > 1:440411/1‑251 (MQ=255) cGAGAATGTGATGGAAGAGTATCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGTAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGTCATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCt < 2:514076/251‑1 (MQ=255) tgtgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTAc > 2:139363/1‑251 (MQ=255) gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGATGAATCTGATTActtc < 1:602098/251‑1 (MQ=255) gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGGTGTACATAACCA‑CGATTTTGACTCGGCGCAGTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGATGAATCGGATTActtc < 2:309447/251‑1 (MQ=255) ggAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTActtcctt > 2:388775/1‑251 (MQ=255) agagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCCATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAGGGTGGGGGGGATAGATTAGTTGTACAA‑ACCAACGATTTGGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCTTTTGTACGGGGGCGAATAAAAGGAGGAATTGGATAACTTCCTTCa > 1:443696/1‑250 (MQ=255) cATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATg < 1:420005/251‑1 (MQ=255) gCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg < 2:366629/251‑1 (MQ=255) gCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCCGCAATACGGAGGATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGAGTAGTTGTAAATAACCA‑CGATGTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg < 1:74624/251‑1 (MQ=255) cTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAACGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg > 1:189018/1‑250 (MQ=255) tttGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTgcaa > 2:117305/1‑245 (MQ=255) ttacTGAGCGGATGAATCAGCACCCGGCGGTACAGCGATTCTGCAATACGGATTATTATGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGGGATAGATTAGGTGTACATAACCC‑CGGTTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATTGACGCGGGCGAATAAATAGAGGACTCTTATTACTTCCTTCATGGGGATGCTGAAAag > 2:338612/4‑249 (MQ=255) ttGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGTTGCTGAAAag > 2:494278/1‑249 (MQ=255) tGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGTATGCTGAAAagag < 1:120639/250‑1 (MQ=255) gCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATAAGGCTTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATCACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGCTGCTGAAAAGagt > 1:254604/1‑250 (MQ=255) cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTCGTCAAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGagt > 1:357536/1‑249 (MQ=255) cGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTCTTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGaataaa > 1:388183/1‑200 (MQ=255) cGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTGGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGTGCGaataaa < 2:388183/200‑1 (MQ=255) gatgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTACGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa > 2:597083/1‑247 (MQ=255) gatgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa < 1:597083/247‑1 (MQ=255) attatTCAGCACCCGGAGGTACAGCGAATCTGCAATACGGATTAGTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGTCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCt < 2:347940/248‑1 (MQ=255) atTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt > 1:369688/1‑248 (MQ=255) cACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccata < 1:62276/251‑10 (MQ=255) gCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcg > 1:609315/1‑228 (MQ=255) gAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACCTCCCC‑CCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATAACAA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATCAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcgt > 1:569871/1‑226 (MQ=255) acctccccc‑c‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc > 1:307949/6‑104 (MQ=255) acctccccc‑c‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc < 2:307949/99‑1 (MQ=255) | CAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGCTCCCC‑C‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA > NC_000913/19430‑19819 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |