Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	19,613	T→C	V300A (GTG→GCG)	nhaR →	DNA‑binding transcriptional activator NhaR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	19,613	0	T	C	100.0%	142.4 / NA	53	V300A (GTG→GCG)	nhaR	DNA‑binding transcriptional activator NhaR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/26);  total (27/26)

AAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGC‑TCCCC‑C‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19370‑19819                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                   aaaTCCTCGGCGAGTTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTaa                                                                                                                                                                                                             <  1:124373/251‑1 (MQ=255)   ctCCTCGGCGAGTTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTCCCATGATATTTTTGCTGAGCGGATGCTTCAGCACCCGGCGGTACAGCGAATCTTCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGGTAAtt                                                                                                                                                                                                           >  2:4858/2‑250 (MQ=255)     ccTCGGCGAGTTTTATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCg                                                                                                                                                                                                          <  1:481299/250‑1 (MQ=255)      ctaggCGAGTTTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGGGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTAAAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGc                                                                                                                                                                                                        >  2:25066/4‑251 (MQ=255)         ggCGAGTTTGATGATGCCGCGTTGATGACAGCTTTGGGTGAGATGCACAATGCAATATTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGACTGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCGGCACCCGGCGGTACAGCGAATCTGCAATACGGATCATTCTGCGCTTTTTAGTCCAGCGGCGCGTTACTCGGcgcc                                                                                                                                                                                                     >  2:62276/1‑248 (MQ=255)          gCGAGATTGATGATGCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGACGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCAAGCGGCGCGTTAATCGGCacct                                                                                                                                                                                                    >  1:261073/1‑248 (MQ=255)                      atgCCGCGTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGtt                                                                                                                                                                                      <  2:439894/251‑1 (MQ=255)                           gcgttGATTAAAGCTTTTGGTGCGATTCACTATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCAGATAAAACTGTCTTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATTCGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTaatgt                                                                                                                                                                                 <  2:362333/248‑4 (MQ=255)                             gttGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTgg                                                                                                                                                                               <  2:23799/250‑1 (MQ=255)                             gttGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTgg                                                                                                                                                                               <  1:383115/250‑1 (MQ=255)                                 atgaAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAATTGAAGGTTGGGga                                                                                                                                                                           <  2:264988/251‑1 (MQ=255)                                        cTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGGGGGGGagatagct                                                                                                                                                                    >  2:195660/1‑249 (MQ=255)                                               tGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGt                                                                                                                                                             >  2:518009/1‑251 (MQ=255)                                                    tGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACAt                                                                                                                                                         >  1:587776/1‑250 (MQ=255)                                                         aaTGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTGTAGTCCATCGGCGCGTTAATCGGCACCGCCCCC‑C‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACAtatccac                                                                                                                                                   <  1:380564/251‑7 (MQ=255)                                                             cAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTATTTGTACATAACCA‑CGAtt                                                                                                                                              <  2:488411/251‑1 (MQ=255)                                                                         ccccAACGCTTTATGCATATGCCTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGAGGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGTGTGAGATAGATGAGTTGTACATACCCA‑CGATTTTGACTCGGCt                                                                                                                                   <  2:416532/250‑1 (MQ=255)                                                                          cccAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCa                                                                                                                                 <  1:238209/251‑1 (MQ=255)                                                                                   ttATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTg                                                                                                                          <  2:312580/249‑1 (MQ=255)                                                                                             taCTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATGTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCTGCACC‑TCCCCGC‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTGCATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTgaga                                                                                                               <  1:591807/249‑1 (MQ=255)                                                                                              aCTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGAc                                                                                                              >  1:342446/1‑250 (MQ=255)                                                                                                       ccGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGAGAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTTAGACATTATTTCCa                                                                                                    <  1:59559/251‑1 (MQ=255)                                                                                                          aTAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTa                                                                                                                                                                                     >  1:579263/1‑168 (MQ=255)                                                                                                          aTAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTGTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCGTCAATACGTATGATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTa                                                                                                                                                                                     <  2:579263/168‑1 (MQ=255)                                                                                                            aaaaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGt                                                                                               >  1:452022/1‑251 (MQ=255)                                                                                                              aaCTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTAc                                                                                             >  2:504112/1‑251 (MQ=255)                                                                                                                             tGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCACGCTTGAGACATTATTTCAATATGTACGCGGGCGaataaat                                                                               >  2:66654/1‑250 (MQ=255)                                                                                                                                 cgcgTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAgg                                                                          >  1:440411/1‑251 (MQ=255)                                                                                                                                      cGAGAATGTGATGGAAGAGTATCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGTAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGTCATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCt                                                                     <  2:514076/251‑1 (MQ=255)                                                                                                                                            tgtgATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTAc                                                               >  2:139363/1‑251 (MQ=255)                                                                                                                                               gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGATGAATCTGATTActtc                                                            <  1:602098/251‑1 (MQ=255)                                                                                                                                               gATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGGTGTACATAACCA‑CGATTTTGACTCGGCGCAGTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGATGAATCGGATTActtc                                                            <  2:309447/251‑1 (MQ=255)                                                                                                                                                  ggAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTActtcctt                                                         >  2:388775/1‑251 (MQ=255)                                                                                                                                                     agagTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCCATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAGGGTGGGGGGGATAGATTAGTTGTACAA‑ACCAACGATTTGGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCTTTTGTACGGGGGCGAATAAAAGGAGGAATTGGATAACTTCCTTCa                                                       >  1:443696/1‑250 (MQ=255)                                                                                                                                                            cATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATg                                               <  1:420005/251‑1 (MQ=255)                                                                                                                                                               gCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg                                            <  2:366629/251‑1 (MQ=255)                                                                                                                                                               gCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCCGCAATACGGAGGATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGAGTAGTTGTAAATAACCA‑CGATGTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg                                            <  1:74624/251‑1 (MQ=255)                                                                                                                                                                cTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAACGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTg                                            >  1:189018/1‑250 (MQ=255)                                                                                                                                                                     tttGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTgcaa                                         >  2:117305/1‑245 (MQ=255)                                                                                                                                                                      ttacTGAGCGGATGAATCAGCACCCGGCGGTACAGCGATTCTGCAATACGGATTATTATGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGGGATAGATTAGGTGTACATAACCC‑CGGTTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATTGACGCGGGCGAATAAATAGAGGACTCTTATTACTTCCTTCATGGGGATGCTGAAAag                                       >  2:338612/4‑249 (MQ=255)                                                                                                                                                                      ttGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGTTGCTGAAAag                                       >  2:494278/1‑249 (MQ=255)                                                                                                                                                                       tGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGTATGCTGAAAagag                                     <  1:120639/250‑1 (MQ=255)                                                                                                                                                                        gCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATAAGGCTTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATCACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGCTGCTGAAAAGagt                                    >  1:254604/1‑250 (MQ=255)                                                                                                                                                                         cTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTCGTCAAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGagt                                    >  1:357536/1‑249 (MQ=255)                                                                                                                                                                              cGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTCTTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGaataaa                                                                                >  1:388183/1‑200 (MQ=255)                                                                                                                                                                              cGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTGGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGTGCGaataaa                                                                                <  2:388183/200‑1 (MQ=255)                                                                                                                                                                                gatgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTACGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa                               >  2:597083/1‑247 (MQ=255)                                                                                                                                                                                gatgatTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTaa                               <  1:597083/247‑1 (MQ=255)                                                                                                                                                                                 attatTCAGCACCCGGAGGTACAGCGAATCTGCAATACGGATTAGTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGTCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCt                          <  2:347940/248‑1 (MQ=255)                                                                                                                                                                                    atTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCtttt                       >  1:369688/1‑248 (MQ=255)                                                                                                                                                                                          cACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccata                 <  1:62276/251‑10 (MQ=255)                                                                                                                                                                                                        gCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATAACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcg   >  1:609315/1‑228 (MQ=255)                                                                                                                                                                                                          gAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGCGCGTTAATCGGCACC‑TCCCC‑CCAAAGTTAAGGCGGGGGAGATAGATTAGTTGTACATAACAA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTATTTCCATATGTACGCGGGCGAATCAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCttttaccatactggatttcattcgt  >  1:569871/1‑226 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                   AAATCCTCGGCGAGTTTGATGATGCCGCTTTGATGAAAGCTTTTGGTGCGATGCACAATGCAATCTTCGTTGCCCCAACGCTTTATGCATATGACTTTTATGCCGATAAAACTGTCGTAGAAATTGGTCGCGTCGAGAATGTGATGGAAGAGTACCATGCTATTTTTGCTGAGCGGATGATTCAGCACCCGGCGGTACAGCGAATCTGCAATACGGATTATTCTGCGCTTTTTAGTCCAGCGGTGCGTTAATCGGCAGC‑TCCCC‑C‑AAAGTTAAGGTGGGGGAGATAGATTAGTTGTACATTACCA‑CGATTTTGACTCGGCTCATTATTTGCCCGCTTGAGACATTGTTTCCATATGTACGCGGGCGAATAAATAGAGGAATCTGATTACTTCCTTCATGGGGATGCTGAAAAGAGTAGTAATTGCTGGTAATGACTCCAACTTATTGATA  >  NC_000913/19370‑19819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑