Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	18,477	T→C	V330A (GTG→GCG)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	18,477	0	T	C	100.0%	143.1 / NA	45	V330A (GTG→GCG)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (12/33);  total (12/33)

CGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGTGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATG  >  NC_000913/18231‑18723                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                            cgaagcgaCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTAtggcgg‑tg                                                                                                                                                                                                                                                        >  1:334600/1‑251 (MQ=255)   aagcgaCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑Tggg                                                                                                                                                                                                                                                      <  2:476453/251‑1 (MQ=255)   aagcgaCTGGAGCATGTGTTAAACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTGCACTGCAAGGTGTCACGCTGGATGGCTTGACCGCCATTCTGCCATTGGGGAGCAACGCTGGCTTGCTGATTGGCAAACCGCTGGTGATGAGTCTGTTCTGCTGTTTGGCGCTGCGTTTGAAACGGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑Tggg                                                                                                                                                                                                                                                      <  1:52279/251‑1 (MQ=255)      cgaCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑Tggggtg                                                                                                                                                                                                                                                   >  1:568070/1‑249 (MQ=255)              gCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCg                                                                                                                                                                                                                                          <  1:115999/251‑1 (MQ=255)               cATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGT‑CCTGTGCgg                                                                                                                                                                                                                                         >  1:258113/1‑251 (MQ=255)                      tacACCCGTGTGTGGCGTATCTGCTTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTAACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTAtggcgg‑tggtgcgccgatgggatgagcgt                                                                                                                                                                                                                                   >  2:276525/3‑231 (MQ=255)                        cACCCGGGGGTGGCGTAGCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGGGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑ttt                                                                                                                                                                                                                               <  2:342924/251‑1 (MQ=255)                         aCCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGT‑CCTGGGCggggacggtgtt                                                                                                                                                                                                                               >  1:551666/1‑241 (MQ=255)                          cccGTGGGTGGCGTATTTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTa                                                                                                                                                                                                                             <  1:112280/251‑1 (MQ=255)                              tgggtggCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGACATTGGGGATCATCGCTGGCTTGCTGCTTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGGATGGGGAT‑C‑TGTGCGTGAACGG‑TTTTACTAt                                                                                                                                                                                                                         >  1:391727/1‑251 (MQ=255)                                            gATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTg                                                                                                                                                                                                           <  2:598058/251‑1 (MQ=255)                                               tttGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGcc                                                                                                                                                                                                         <  1:462794/250‑1 (MQ=255)                                                  gCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGc                                                                                                                                                                                                      <  2:334600/250‑1 (MQ=255)                                                        gTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGc                                                                                                                                                                                                 <  1:437899/249‑1 (MQ=255)                                                         tttGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TTGGGGT‑CCTGCGGGGGAGCGG‑TTTTTATATGTCTTTCTTTTTTTCCCGACTGGCC‑tt                                                                                                                                                                                             >  1:73807/1‑251 (MQ=255)                                                            gCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAACCCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGT‑CCTGTGCGGTATCGGGTTTTA‑TATGTGTATAtttttttacaccccgccctttgg                                                                                                                                                                                           >  1:589288/1‑231 (MQ=255)                                                                  gCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGG‑CCAGTGCGGGATCGG‑TTTTACCTTATCTATCTTTTTTGCCACCCTGGCC‑TTTTGGAGCGt                                                                                                                                                                                    >  1:628939/1‑251 (MQ=255)                                                                  gCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCg                                                                                                                                                                                     <  2:353244/250‑1 (MQ=255)                                                                              gTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACt                                                                                                                                                                       <  2:483890/251‑1 (MQ=255)                                                                                ttCACTGCAAGGTGGCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTAGGTCTATCTTTATTGCCAGCCTGTCC‑TTTGGGAGCGT‑AGATCCa                                                                                                                                                                            <  2:324972/244‑1 (MQ=255)                                                                                  cACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGAGCATCGCTGGCTTGCTGATTGGCAAACCGCTTGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGAtt                                                                                                                                                                   <  1:327689/251‑1 (MQ=255)                                                                                   aCTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAG‑CCCGGGCGGTGTCGG‑GTTTTTTATGTCTTTTTTTTTTTTCCGCCCGGCC‑TTTGGTAGCGT‑AGATCCacgaatttttt                                                                                                                                                                  >  1:546194/1‑241 (MQ=255)                                                                                     tGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTTTCTTTATTTTCCGCCTGGCC‑CTTGGTAGGGT‑AGATCCAGaaacgctgta                                                                                                                                                                 >  1:223297/1‑242 (MQ=255)                                                                                        aaGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGTACCT‑TGGGGGATCGG‑TTTTTATATGTTTTTCTTTTTTTTCAGCCTGGCCCTTTGGT‑GCGT‑AGATCCAAAACTGGTTAACTgg                                                                                                                                                             >  1:328161/1‑251 (MQ=255)                                                                                           gtgTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGTTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCg                                                                                                                                                          <  2:397277/249‑1 (MQ=255)                                                                                                  gCTGGATGGCTTGACCTCCATTCTTCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAACCCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCTTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGTGGAT‑CCTGTGCTGTATCGG‑TTTTACTATGTCTATCTTTATTGCCATCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCg                                                                                                                                                   <  1:389780/251‑1 (MQ=255)                                                                                                  gCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTTGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACGGATTAACTGGGCGAAACTCg                                                                                                                                                   <  2:403632/251‑1 (MQ=255)                                                                                                              gACCTCTATTCTGCCTGTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTTCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTAGCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTc                                                                                                                                        <  1:203468/250‑1 (MQ=255)                                                                                                                   ccATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGTTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGtt                                                                                                                                    <  1:431370/249‑1 (MQ=255)                                                                                                                           ccATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TGTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCGGGTCGGTTCTATCTCTTc                                                                                                                          <  2:82276/251‑1 (MQ=255)                                                                                                                              ttGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGG‑CCTTGGCGGGATCGG‑TTTTACTTTGTTTTTCTTTTTTTTCAGCCTGGCC‑CTTTGTAG‑GTGAGAACCAGAACTGTTTATATTGGCGGAAATCGGTTTCCTGGGCgggtttttcttttcgtc                                                                                                                       >  2:196645/1‑236 (MQ=255)                                                                                                                                                     gATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGGT‑CCTGTGGGGGGTCGG‑GTTTACTATGTCTATCTTTTTTTCCAGCCTGGCC‑TTTTGGGGCGT‑AGATTCCCAACTGTTTTAATAGGCGAGAATCTGTGTCCTGGTGGGGTCTATATCTTTCGCGGGAATTGGGGACAGCTGGtttt                                                                                                >  2:588536/1‑249 (MQ=255)                                                                                                                                                            aaaCCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGcgttcg                                                                                         <  1:17560/251‑1 (MQ=255)                                                                                                                                                                                gTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGttt                                                                        <  2:148093/248‑1 (MQ=255)                                                                                                                                                                                             gcgcTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTAcc                                                        <  1:588536/251‑1 (MQ=255)                                                                                                                                                                                                  gCGTTTGAAACTGGCGCATCTGCCTGAGGGAACTACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGTACGGttgtccggt                                                     <  2:92170/249‑8 (MQ=255)                                                                                                                                                                                                  gCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGGCCATCAGTTTGACAGGACGGTTTACCGGGGa                                                   <  2:416752/251‑1 (MQ=255)                                                                                                                                                                                                         aaaCTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATaa                                            <  2:211937/251‑1 (MQ=255)                                                                                                                                                                                                                cgcATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTGACCGGGGAGCCATAAACGGCTc                                     <  2:551666/251‑1 (MQ=255)                                                                                                                                                                                                                       gCCTGAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTATATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGGCGGTTGACCGGGGAGCCATAAACGGCTCCCtttt                               <  2:437450/250‑1 (MQ=255)                                                                                                                                                                                                                           gAGGGAACGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑TCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCAtt                           <  2:502197/250‑1 (MQ=255)                                                                                                                                                                                                                               gAACGGCTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTAGCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCAGATACTGCTCCCTTTTCAGTGTTAt                      <  2:77821/251‑1 (MQ=255)                                                                                                                                                                                                                                 aCGACTTATCAGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCa                    <  2:111325/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCATAACTGATTAACTGGGCGAAACTCGGTATCCGGGTCGGTTCTATCTCTTCGGCTGTAAGTGGATACAGCTGGTTACGCGTTCGTTTGCTTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGaaa          <  1:568907/251‑1 (MQ=255)                                                                                                                                                                                                                                           aGCAAATTATGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGaaa          <  1:196645/251‑1 (MQ=255)                                                                                                                                                                                                                                                   aTGGCGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATg  <  1:120067/251‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                            CGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCTGAGGGAACGACTTATCAGCAAATTATGGTGG‑TGGGGAT‑CCTGTGCGGTATCGG‑TTTTACTATGTCTATCTTTATTGCCAGCCTGGCC‑TTTGGTAGCGT‑AGATCCAGAACTGATTAACTGGGCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTACGCGTTCGTTTGCGTCCATCAGTTTGACAGGACGGTTTACCGGGGAGCCATAAACGGCTCCCTTTTCATTGTTATCAGGGAGAGAAATGAGCATG  >  NC_000913/18231‑18723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑