Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	18,169	T→C	T227T (ACT→ACC)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	18,169	0	T	C	100.0%	211.3 / NA	67	T227T (ACT→ACC)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (32/35);  total (32/35)

CGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTC  >  NC_000913/17927‑18404                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            cGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCggg                                                                                                                                                                                                                                     <  1:243645/251‑1 (MQ=255)    gtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCgggggg                                                                                                                                                                                                                                  >  1:459592/1‑250 (MQ=255)      tCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTa                                                                                                                                                                                                                                 <  1:366499/250‑1 (MQ=255)            aGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTc                                                                                                                                                                                                                           <  1:517061/250‑1 (MQ=255)               gcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCg                                                                                                                                                                                                                                       <  2:309690/235‑1 (MQ=255)               gcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCg                                                                                                                                                                                                                                       >  1:309690/1‑235 (MQ=255)                    aggttgttttTATGGCTCTGGCTATGATCCACGATCTTGGGGCCATCATTATCATCGCATTGGTCTACAGTAATGTCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAAGTGCGGTACGCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGTGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGGTCACGCAACCCTGGCGGGGGTAATTGGCGGCTTCttt                                                                                                                                                                                                                  <  2:468936/245‑1 (MQ=255)                       tCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTAtt                                                                                                                                                                                                               >  1:139736/1‑251 (MQ=255)                          gtttGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCTGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTTTCGGCTTCTTTAtt                                                                                                                                                                                                               <  2:110231/247‑1 (MQ=255)                            ttGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCttt                                                                                                                                                                                                          >  1:474583/1‑251 (MQ=255)                              gATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGTCTTCTTTATTCCTTTg                                                                                                                                                                                                         <  1:225729/250‑1 (MQ=255)                               aTGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTACTTGTCGGATTCTTTGTTCCTTTGa                                                                                                                                                                                                        >  2:518449/1‑250 (MQ=255)                                       ggCTATTAGCGACGATCGTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGc                                                                                                                                                                                               <  2:452484/251‑1 (MQ=255)                                            ttatCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATggg                                                                                                                                                                                          >  1:437450/1‑251 (MQ=255)                                              atCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGGGAAGCATGGGCg                                                                                                                                                                                        >  2:112280/1‑251 (MQ=255)                                                      tCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTcc                                                                                                                                                                                  >  1:342924/1‑249 (MQ=255)                                                         tggtgacatcatTATCTTCGGATTGTTCTACACTAAGGACTTATCGAAGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTGGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGTGTTCTCCag                                                                                                                                                                                <  2:510549/242‑1 (MQ=255)                                                         tGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCagc                                                                                                                                                                               <  1:109828/249‑1 (MQ=255)                                                           gggCCATCATTATCACCGCATTGCTCTACACTAATGACTTATCGATGGCCTCTATTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGAATTGAATCTGTGTGGTGTACGCAGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGAGGGGGCAATTGTAGGCTTCTTTATTCCTTTGAAAGAGTAGCATGGGCGTTCTTCagcga                                                                                                                                                                             >  2:568907/1‑249 (MQ=255)                                                                 tcatTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGCATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTg                                                                                                                                                                     >  1:624497/1‑251 (MQ=255)                                                                  catTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTgg                                                                                                                                                                    >  1:397277/1‑251 (MQ=255)                                                                    ttATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGTGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGa                                                                                                                                                                   <  2:225054/250‑1 (MQ=255)                                                                     tatccgcGCATTGTTCTACAATACTGACATATAGATGGCCTCTATTGGAGTCGCGGCTGTAGCAATTGCGGTAATCGCGGTCTTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGATGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTTCTTTGAAAGAGAAGCGTGGGCGGTCTCCAGCGAAGCGACTGGAGc                                                                                                                                                                 >  2:327689/7‑251 (MQ=255)                                                                        catcGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCAtg                                                                                                                                                              >  1:212381/1‑251 (MQ=255)                                                                             cATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGGGtt                                                                                                                                                          >  1:148093/1‑250 (MQ=255)                                                                                 gTTCTACACTAATGACTTAGCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATGCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTacac                                                                                                                                                      <  1:98013/250‑1 (MQ=255)                                                                                        acTAATGGCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATGCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggt                                                                                                                                              <  2:193066/251‑1 (MQ=255)                                                                                           gaTGACTTAGCGAGGGCCTCGCTTGGCGTCGCGGCTGTAGCAATTGCGGTGCTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGGGGACTGCGGTGTTGAAATCGGGGGTTCACGTAGCCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtgg                                                                                                                                            <  1:378661/249‑1 (MQ=255)                                                                                            aTGACTTATCGATGGCCTCTCGTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCGGGTTGTCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCAGGGGCGTTCTCCAGCGAAGCGACTGGAGCATGGGTTACGCCCGGGGGTGGc                                                                                                                                           <  2:605389/250‑1 (MQ=255)                                                                                              gACTTAGCGATGGCCTCTCTTGGCGGCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTAGTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGt                                                                                                                                         <  2:212381/250‑1 (MQ=255)                                                                                                  tATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATc                                                                                                                                      <  2:139736/249‑1 (MQ=255)                                                                                                                    gCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGc                                                                                                                   >  1:353244/1‑250 (MQ=255)                                                                                                                    gCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCa                                                                                                                  <  2:177964/251‑1 (MQ=255)                                                                                                                               gTAGCAATTGCGGTACTCGCGGTAGTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGGTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATg                                                                                                        <  2:454934/250‑1 (MQ=255)                                                                                                                                       tGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGttt                                                                                               >  1:598058/1‑251 (MQ=255)                                                                                                                                              cTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTCTTCCTTTGAAAGAGACGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGAATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGc                                                                                         >  1:92170/1‑250 (MQ=255)                                                                                                                                                   ggTATTGAATCTGTTTGGTGTACGCCTCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTCCGGTGTTTAAATCGGGGGTGCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTGATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCAGGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAggtg                                                                                   <  1:307827/251‑3 (MQ=255)                                                                                                                                                      aTTGAATCTGTGTGGTGTACGACGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTc                                                                                 <  2:481605/250‑1 (MQ=255)                                                                                                                                                         gAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGTCTGGAGCATGTGTTACACCCGTGGGTGGCGTAGCTGAGTTTGCCGCTGTTTGCATGTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACg                                                                              <  2:300563/250‑1 (MQ=255)                                                                                                                                                         gAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGc                                                                             >  1:502197/1‑251 (MQ=255)                                                                                                                                                               gggtggtgtgcgccgcACGGGCGTCTATAGTCTGGTTGGCGTAGTGGTGTGGGCTGCGGTGTTGAAATCGGGGGTTCACGCAACCCGGGCGGGGGTAATTGTCGGCTTCTTGAGTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTAGCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACGGCAAGGTGGCACGCTGGAt                                                                        <  2:57963/240‑1 (MQ=255)                                                                                                                                                                tgtgGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTCGTGTTGTGGACTGCGGTGTTGAAATAGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGACGCATGGGCGTTCTCCAGCGCAGCAACTGGAGCATGTGTTACACCCGGGGGTGGCGCATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGTTgg                                                                      >  1:259654/1‑251 (MQ=255)                                                                                                                                                                      gttcgccgcACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGAc                                                                <  2:351464/248‑1 (MQ=255)                                                                                                                                                                        aCGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGAccc                                                              >  1:176516/1‑250 (MQ=255)                                                                                                                                                                        aCGCCGCACGGGCGGCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCt                                                              <  2:192032/251‑1 (MQ=255)                                                                                                                                                                         cgccgcACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTc                                                             >  1:211937/1‑251 (MQ=255)                                                                                                                                                                         cgccgcACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTc                                                             <  1:269065/251‑1 (MQ=255)                                                                                                                                                                         cgccgcACGGGCGGCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGGTCACGCACCCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGGTTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCCCTGCACGGTGTCACGCTGGATAGCTTGACCTc                                                             >  2:203468/1‑251 (MQ=255)                                                                                                                                                                                 gggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGc                                                     >  1:416752/1‑251 (MQ=255)                                                                                                                                                                                  ggCGTCTATATTCTGGTTGGCGTAGGGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCAAGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTGTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGGGGCGTATCTGATTTTGCCGCTGGTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGcc                                                    <  1:269607/251‑1 (MQ=255)                                                                                                                                                                                    cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCAt                                                  <  2:474583/251‑1 (MQ=255)                                                                                                                                                                                     gTCTATATTATGGTTGGCGAAGTGTAGTGGACTGCGGTGTTGAAATAGGGGGTTCACGCAACCCTGGCGGGGGTAATTGCCGGCTTCTTTATTCATTTGAAAGAGAAGCATGGGCGTTCTCCCGCGAAGCGCCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGCTTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACGGCAAGGTGTCACGCTGGATGGCTTGGCCTCCCTTCTGCCAtt                                                 >  2:431370/1‑251 (MQ=255)                                                                                                                                                                                             tctggttgGAGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGatct                                         >  1:110838/5‑250 (MQ=255)                                                                                                                                                                                                   tgGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGAGTTCACGCAACCCTGGCGGGGGTAGTTGTCGGCTTCTTTATTCCTTTGAAAGAGACGCCTGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGGTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCCTTTGTTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTTACCTCCATTCTGCCATTGGGGATCATCGCTg                                   >  2:304199/1‑251 (MQ=255)                                                                                                                                                                                                   tgGCGTAGTGATGGGGACTGCGGTGTGGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATAGACGGCTACATTATTTATTTGAAAGAGAAGCATGGGCGTTATCCAGCGAAGAGACTGGAGCATGGGTTACACCCGTGGGGGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGGTGGCTTGACCTCCATTCTGCCATTGGGGATCATAGCTg                                   >  2:389780/1‑251 (MQ=255)                                                                                                                                                                                                         agtgtTGTGGACTGCGGTGTTGACATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGc                             >  1:178014/2‑251 (MQ=255)                                                                                                                                                                                                           tgttgtGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTTTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCTGGGGAGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGGTGGCGTTTCACTGCAAGGTGTCACGCTGGGTGGCTTGACCTCCATTCTGCCCTTGGGGATCATCGCTGGCTTGCTg                           >  2:437899/1‑251 (MQ=255)                                                                                                                                                                                                           tgttgtGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGAAATTGTCGGCTTCTTTCTTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTTCATTTGCTAATGCTGGCGTTTCACTGCACGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTg                           >  1:400661/1‑251 (MQ=255)                                                                                                                                                                                                              tgtgGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCGGGCTTGCTGAt                         <  2:624497/250‑1 (MQ=255)                                                                                                                                                                                                                   aCTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCa                    >  1:74327/1‑250 (MQ=255)                                                                                                                                                                                                                      gCGGTGTTGAAATCGGGTGTTCACTCACCCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGGTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGTGTGGCGTAGCTGATGTTGCCGCGGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTGGCTGATGGGCAAAcc                <  2:605987/251‑1 (MQ=255)                                                                                                                                                                                                                       gggTGTTGAAAATGGGGGTTCACGCAACCATGGCGGGTGTAATTGTCGGCTCCTTTAGTCCTTTGAAAGAGAAGCATGGGCGGTCTCCAGCGAAGCGAATGGAGCATGTGTGACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCGGGCGTTTCACGGTAAGGTGTCACGCTGGATGGCTGGACCTCTATTCTGCCACTGGGGATCATCGCCGGCTTGCTGCGTGGCAAAcct               <  2:331329/250‑2 (MQ=255)                                                                                                                                                                                                                           gtggtaaTCGTGGGTTCACTCAACCCTTGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAATCATGGTCTTTCTCTAGCTAAGCGACTGGAGCATTTGTTACACCCGTGGTTGGCGTAGCTGATTTTGCCGCTGTTTGCATTTGCAAATGCTGGCTTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTTTGGATCATCGCTGGCTTGCTGTTTGGAAAACCGCTg            <  2:464891/245‑1 (MQ=255)                                                                                                                                                                                                                           gtTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTg            <  1:518449/250‑1 (MQ=255)                                                                                                                                                                                                                             tGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTggg          <  2:74327/250‑1 (MQ=255)                                                                                                                                                                                                                                   cGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTTGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGTGATTAg    <  1:1493/250‑1 (MQ=255)                                                                                                                                                                                                                                    gggggTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTc  >  1:297944/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            CGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAACCGCTGGGGATTAGTC  >  NC_000913/17927‑18404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑