Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 18,127 G→A V213V (GTG→GTA)  nhaA → Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091318,1270GA98.6% 217.9 / NA 70V213V (GTG→GTA) nhaANa(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (32/37);  minor base C (1/0);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 4.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTG  >  NC_000913/17882‑18371
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
aCTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAgtgt                                                                                                                                                                                                                                                  <  1:258118/250‑1 (MQ=255)
      aTTGCCTTTGCACTTGGTGTGCATGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGACTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTggcc                                                                                                                                                                                                                                           >  2:366499/1‑249 (MQ=255)
          cctttGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGCATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCg                                                                                                                                                                                                                                       >  2:115999/3‑251 (MQ=255)
             ttGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGGTCCGGTAGCGCTGAAGATCTTTTTGATTGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATGGTTCTACACTAAGGACTGATCGATGTCCTCTCGTGGCGTCGCGGCTGTAGCAATTGCTGTACTCGCGGTATTGAATCTGTGTGGTGTACTCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCgg                                                                                                                                                                                                                                      <  1:619784/249‑1 (MQ=255)
             ttGCACTTGGTGCGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGAGCTTGGGGCCATCATTATCATCGCATTGTTCGCCACGAATGACTTATCGATGGCCTCTCTTTGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGTGCGTCTATATTCTGGTTGGCGTAgtgtagggaacgagtgt                                                                                                                                                                                                                                     <  2:332984/250‑14 (MQ=255)
              tGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGgtg                                                                                                                                                                                                                                    <  2:617564/250‑1 (MQ=255)
                 aCTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGACGCTCTTTTTGATGGCTCTGGCTATTATCTACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGTTGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTCTTGCATCTGTGTGGTGTACGCCGCACGGGAGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTg                                                                                                                                                                                                                                 >  1:605987/1‑250 (MQ=255)
                    tGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAAt                                                                                                                                                                                                                             >  1:476453/1‑251 (MQ=255)
                    tGGTGTGCTGGCGCTGTTGGGAAGGCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGGCTGATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATGCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGaa                                                                                                                                                                                                                               <  2:83457/249‑1 (MQ=255)
                        gtgcTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCgg                                                                                                                                                                                                                          <  2:527294/247‑1 (MQ=255)
                            tGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGtt                                                                                                                                                                                                                     >  1:234549/1‑251 (MQ=255)
                             ggCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTTGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAAGCTGTGTGGTGTACGCCGCACGGGCGTCTATATGCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGt                                                                                                                                                                                                                      <  2:207497/249‑1 (MQ=255)
                                       ggAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAAccct                                                                                                                                                                                                          <  2:522892/251‑4 (MQ=255)
                                             cGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCggg                                                                                                                                                                                                    <  1:243645/251‑1 (MQ=255)
                                                gtTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCgggggg                                                                                                                                                                                                 >  1:459592/1‑250 (MQ=255)
                                                  tCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTa                                                                                                                                                                                                <  1:366499/250‑1 (MQ=255)
                                                        aGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTc                                                                                                                                                                                          <  1:517061/250‑1 (MQ=255)
                                                           gcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCTTTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATCTTCTGGTTGGCGTAGTGTTGTGGCCTGCGGTGTTGAAATCgg                                                                                                                                                                                                                          >  2:21078/1‑215 (MQ=255)
                                                           gcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCgg                                                                                                                                                                                                                          <  1:21078/215‑1 (MQ=255)
                                                           gcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCg                                                                                                                                                                                                      <  2:309690/235‑1 (MQ=255)
                                                           gcTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCg                                                                                                                                                                                                      >  1:309690/1‑235 (MQ=255)
                                                                aggttgttttTATGGCTCTGGCTATGATCCACGATCTTGGGGCCATCATTATCATCGCATTGGTCTACAGTAATGTCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAAGTGCGGTACGCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGTGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGGTCACGCAACCCTGGCGGGGGTAATTGGCGGCTTCttt                                                                                                                                                                                 <  2:468936/245‑1 (MQ=255)
                                                                   tCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTAtt                                                                                                                                                                              >  1:139736/1‑251 (MQ=255)
                                                                      gtttGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCTGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTTTCGGCTTCTTTAtt                                                                                                                                                                              <  2:110231/247‑1 (MQ=255)
                                                                        ttGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCttt                                                                                                                                                                         >  1:474583/1‑251 (MQ=255)
                                                                          gATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGTCTTCTTTATTCCTTTg                                                                                                                                                                        <  1:225729/250‑1 (MQ=255)
                                                                           aTGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTACTTGTCGGATTCTTTGTTCCTTTGa                                                                                                                                                                       >  2:518449/1‑250 (MQ=255)
                                                                                   ggCTATTAGCGACGATCGTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGc                                                                                                                                                              <  2:452484/251‑1 (MQ=255)
                                                                                        ttatCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATggg                                                                                                                                                         >  1:437450/1‑251 (MQ=255)
                                                                                          atCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGGGAAGCATGGGCg                                                                                                                                                       >  2:112280/1‑251 (MQ=255)
                                                                                                  tCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTcc                                                                                                                                                 >  1:342924/1‑249 (MQ=255)
                                                                                                     tggtgacatcatTATCTTCGGATTGTTCTACACTAAGGACTTATCGAAGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTGGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGTGTTCTCCag                                                                                                                                               <  2:510549/242‑1 (MQ=255)
                                                                                                     tGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCagc                                                                                                                                              <  1:109828/249‑1 (MQ=255)
                                                                                                       gggCCATCATTATCACCGCATTGCTCTACACTAATGACTTATCGATGGCCTCTATTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGAATTGAATCTGTGTGGTGTACGCAGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGAGGGGGCAATTGTAGGCTTCTTTATTCCTTTGAAAGAGTAGCATGGGCGTTCTTCagcga                                                                                                                                            >  2:568907/1‑249 (MQ=255)
                                                                                                             tcatTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGCATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTg                                                                                                                                    >  1:624497/1‑251 (MQ=255)
                                                                                                              catTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGGAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTgg                                                                                                                                   >  1:397277/1‑251 (MQ=255)
                                                                                                                ttATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGTGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGa                                                                                                                                  <  2:225054/250‑1 (MQ=255)
                                                                                                                 tatccgcGCATTGTTCTACAATACTGACATATAGATGGCCTCTATTGGAGTCGCGGCTGTAGCAATTGCGGTAATCGCGGTCTTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGATGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTTCTTTGAAAGAGAAGCGTGGGCGGTCTCCAGCGAAGCGACTGGAGc                                                                                                                                >  2:327689/7‑251 (MQ=255)
                                                                                                                    catcGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCAtg                                                                                                                             >  1:212381/1‑251 (MQ=255)
                                                                                                                         cATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGGGtt                                                                                                                         >  1:148093/1‑250 (MQ=255)
                                                                                                                             gTTCTACACTAATGACTTAGCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATGCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTacac                                                                                                                     <  1:98013/250‑1 (MQ=255)
                                                                                                                                    acTAATGGCTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATGCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggt                                                                                                             <  2:193066/251‑1 (MQ=255)
                                                                                                                                       gaTGACTTAGCGAGGGCCTCGCTTGGCGTCGCGGCTGTAGCAATTGCGGTGCTCGCGGTATTGAATCTGTGTGGTGGACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGGGGACTGCGGTGTTGAAATCGGGGGTTCACGTAGCCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCgtgggtgg                                                                                                           <  1:378661/249‑1 (MQ=255)
                                                                                                                                        aTGACTTATCGATGGCCTCTCGTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCGGGTTGTCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCAGGGGCGTTCTCCAGCGAAGCGACTGGAGCATGGGTTACGCCCGGGGGTGGc                                                                                                          <  2:605389/250‑1 (MQ=255)
                                                                                                                                          gACTTAGCGATGGCCTCTCTTGGCGGCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTAGTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGt                                                                                                        <  2:212381/250‑1 (MQ=255)
                                                                                                                                              tATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATc                                                                                                     <  2:139736/249‑1 (MQ=255)
                                                                                                                                                                gCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGc                                                                                  >  1:353244/1‑250 (MQ=255)
                                                                                                                                                                gCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCa                                                                                 <  2:177964/251‑1 (MQ=255)
                                                                                                                                                                           gTAGCAATTGCGGTACTCGCGGTAGTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGGTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATg                                                                       <  2:454934/250‑1 (MQ=255)
                                                                                                                                                                                   tGCGGTACTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGttt                                                              >  1:598058/1‑251 (MQ=255)
                                                                                                                                                                                          cTCGCGGTATTGAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTCTTCCTTTGAAAGAGACGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGAATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGc                                                        >  1:92170/1‑250 (MQ=255)
                                                                                                                                                                                               ggTATTGAATCTGTTTGGTGTACGCCTCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTCCGGTGTTTAAATCGGGGGTGCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTGATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCAGGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAggtg                                                  <  1:307827/251‑3 (MQ=255)
                                                                                                                                                                                                  aTTGAATCTGTGTGGTGTACGACGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTc                                                <  2:481605/250‑1 (MQ=255)
                                                                                                                                                                                                     gAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGTCTGGAGCATGTGTTACACCCGTGGGTGGCGTAGCTGAGTTTGCCGCTGTTTGCATGTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACg                                             <  2:300563/250‑1 (MQ=255)
                                                                                                                                                                                                     gAATCTGTGTGGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGc                                            >  1:502197/1‑251 (MQ=255)
                                                                                                                                                                                                           gggtggtgtgcgccgcACGGGCGTCTATAGTCTGGTTGGCGTAGTGGTGTGGGCTGCGGTGTTGAAATCGGGGGTTCACGCAACCCGGGCGGGGGTAATTGTCGGCTTCTTGAGTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTAGCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACGGCAAGGTGGCACGCTGGAt                                       <  2:57963/240‑1 (MQ=255)
                                                                                                                                                                                                            tgtgGTGTACGCCGCACGGGCGTCTATATTCTGGTTGGCGTCGTGTTGTGGACTGCGGTGTTGAAATAGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGACGCATGGGCGTTCTCCAGCGCAGCAACTGGAGCATGTGTTACACCCGGGGGTGGCGCATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGTTgg                                     >  1:259654/1‑251 (MQ=255)
                                                                                                                                                                                                                  gttcgccgcACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGAc                               <  2:351464/248‑1 (MQ=255)
                                                                                                                                                                                                                    aCGCCGCACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGAccc                             >  1:176516/1‑250 (MQ=255)
                                                                                                                                                                                                                    aCGCCGCACGGGCGGCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTGTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCt                             <  2:192032/251‑1 (MQ=255)
                                                                                                                                                                                                                     cgccgcACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTc                            <  1:269065/251‑1 (MQ=255)
                                                                                                                                                                                                                     cgccgcACGGGCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTc                            >  1:211937/1‑251 (MQ=255)
                                                                                                                                                                                                                     cgccgcACGGGCGGCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGGTCACGCACCCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGGTTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCCCTGCACGGTGTCACGCTGGATAGCTTGACCTc                            >  2:203468/1‑251 (MQ=255)
                                                                                                                                                                                                                             gggCGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGc                    >  1:416752/1‑251 (MQ=255)
                                                                                                                                                                                                                              ggCGTCTATATTCTGGTTGGCGTAGGGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCAAGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTGTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGGGGCGTATCTGATTTTGCCGCTGGTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGcc                   <  1:269607/251‑1 (MQ=255)
                                                                                                                                                                                                                                cGTCTATATTCTGGTTGGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTGCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCAt                 <  2:474583/251‑1 (MQ=255)
                                                                                                                                                                                                                                 gTCTATATTATGGTTGGCGAAGTGTAGTGGACTGCGGTGTTGAAATAGGGGGTTCACGCAACCCTGGCGGGGGTAATTGCCGGCTTCTTTATTCATTTGAAAGAGAAGCATGGGCGTTCTCCCGCGAAGCGCCTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGCTTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACGGCAAGGTGTCACGCTGGATGGCTTGGCCTCCCTTCTGCCAtt                >  2:431370/1‑251 (MQ=255)
                                                                                                                                                                                                                                         tctggttgGAGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGatct        >  1:110838/5‑250 (MQ=255)
                                                                                                                                                                                                                                               tgGCGTAGTGTTGTGGACTGCGGTGTTGAAATCGGGAGTTCACGCAACCCTGGCGGGGGTAGTTGTCGGCTTCTTTATTCCTTTGAAAGAGACGCCTGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGGTACACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCCTTTGTTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGATGGCTTTACCTCCATTCTGCCATTGGGGATCATCGCTg  >  2:304199/1‑251 (MQ=255)
                                                                                                                                                                                                                                               tgGCGTAGTGATGGGGACTGCGGTGTGGAAATCGGGGGTTCACGCAACCCTGGCGGGGGTAATAGACGGCTACATTATTTATTTGAAAGAGAAGCATGGGCGTTATCCAGCGAAGAGACTGGAGCATGGGTTACACCCGTGGGGGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGTGTCACGCTGGGTGGCTTGACCTCCATTCTGCCATTGGGGATCATAGCTg  >  2:389780/1‑251 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                    
ACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGTGGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTGAAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAAGAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCGTATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACGCTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTG  >  NC_000913/17882‑18371

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑