Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,809	G→T	V107V (GTG→GTT)	nhaA →	Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,809	0	G	T	100.0%	202.6 / NA	62	V107V (GTG→GTT)	nhaA	Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/27);  total (35/27)

GATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTC  >  NC_000913/17563‑18046                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              gatgatTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTTGTTCACTCGAAATCAACAACAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTATTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGTATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGTGATGATTGTTCCgg                                                                                                                                                                                                                                           <  2:78231/251‑1 (MQ=255) gatgatTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCgg                                                                                                                                                                                                                                           <  1:420499/251‑1 (MQ=255)    gatTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTTATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCAt                                                                                                                                                                                                                                        <  2:39926/251‑1 (MQ=255)             caataGCGGCGCAACCCGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGCTGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCTGTTATCGCCGCTATTGGGGGGATGATTGTTCCGGCATTACTTTAtt                                                                                                                                                                                                                               >  2:312838/5‑250 (MQ=255)                                ggatcatATCACGCCTTTCTCGAGATGCCGGTTCACCTCAGGGTTCGATCATTCGCCATCACCAAAATGATCCTGTTATGGATAAATGTCGCGCTGTTGGCGGTCTTTTTCCTGTTTGTCGGTCTGGGCGTTAAAAGAGCACTGGTGAACGGCTCGGCCGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTTTTGGCGGGGTGATGGTTCCGGCATTACTCATTCTGGCCTTTAACTATGccgc                                                                                                                                                                                                            >  2:619784/7‑250 (MQ=255)                                 gatgccaTCACGCCTGTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAATTCAACAAACACCTGCTGTTATGGATAAATGGCGCGCTGATGGCGGAATTTTTCCTGATAGACGGTCTGGCAGTTAAAAGTGAACTGATGCAAGGCACTCTAGACCGCTTACGACAGGCCGAATTTTCAGCTATCGCCGCTGTTGGTGGGATGATTGTTACGGCATTACTCTATCTGGCCTTTTACTCTGccgat                                                                                                                                                                                                           >  2:327972/7‑251 (MQ=255)                                    ggTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgatccg                                                                                                                                                                                                        <  1:374203/251‑1 (MQ=255)                                     gTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgatccga                                                                                                                                                                                                       <  1:6187/251‑1 (MQ=255)                                       aTCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTGCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGccgatccgat                                                                                                                                                                                                      <  1:566583/250‑1 (MQ=255)                                            gACTTTCTGGATACGCCGGGTCATCTCCGGGTTGGTTCACGCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCTGTCTGGAAGTGAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCcg                                                                                                                                                                                                <  1:570014/251‑1 (MQ=255)                                            gACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCAATCTGGCCTTTAACTATGCCGATCCGATTACCcg                                                                                                                                                                                                >  1:341198/1‑251 (MQ=255)                                              cTTTCTGGAGCCGCCGGTTAAGCACCGGGTTGGTTCACTCGAAATCAACACAAACCTGAAGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGCAGTTAAACGTGAACTGCTGCCAGGCTCGCTAGCCAGCTTCCGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGGGGGATGATTGTTCCGGCATTACTCTATCTGGCATTTAACTATGCCGATCCGATTACCcgcg                                                                                                                                                                                              >  2:258118/1‑251 (MQ=255)                                                     gagaCGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTg                                                                                                                                                                                       <  1:612077/251‑1 (MQ=255)                                                                   gCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCcggcgg                                                                                                                                                                         <  1:116922/251‑1 (MQ=255)                                                                                     cGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTg                                                                                                                                                       >  1:522892/1‑251 (MQ=255)                                                                                                    cATGCTGTTATGGCTAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCCAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTCACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGCCATTGCCCTTGCACTTGGTGTGCTgg                                                                                                                                        >  2:35596/1‑251 (MQ=255)                                                                                                     aTGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCg                                                                                                                                                                                                                                            <  2:471523/150‑1 (MQ=255)                                                                                                     aTGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCg                                                                                                                                                                                                                                            >  1:471523/1‑150 (MQ=255)                                                                                                         tGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAGGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTg                                                                                                                                   >  1:110231/1‑251 (MQ=255)                                                                                                            tATGGATAAATTGCGCCTTGAAGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTATCATGCTTACGCCAGGCCGCATTTCCAGTTATCGCCTCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTCACTATGCCGATCCGATTATCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGTTGTGCTGGCGCTGtt                                                                                                                                 <  2:342360/250‑1 (MQ=255)                                                                                                             aTGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTg                                                                                                                                <  1:160993/250‑1 (MQ=255)                                                                                                               ggATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTggg                                                                                                                              >  1:481605/1‑250 (MQ=255)                                                                                                               ggATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTggg                                                                                                                              >  1:225054/1‑250 (MQ=255)                                                                                                                 aTAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGa                                                                                                                             >  1:193066/1‑249 (MQ=255)                                                                                                                   aaaTGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGGGCTGGCGCTGTTGGGAAGTc                                                                                                                         >  1:189769/1‑251 (MQ=255)                                                                                                                   aaaTGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGACAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGATATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGt                                                                                                                          <  2:141432/250‑1 (MQ=255)                                                                                                                                         tttttCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACt                                                                                                                                                                    >  1:150169/1‑186 (MQ=255)                                                                                                                                         tttttCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACt                                                                                                                                                                    <  2:150169/186‑1 (MQ=255)                                                                                                                                              ccTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCtttt                                                                                              >  1:617564/1‑251 (MQ=255)                                                                                                                                                 gTTAGTCGGTCTGGAAGTTAAACGTTAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTg                                                                                            <  1:346816/250‑1 (MQ=255)                                                                                                                                                       cGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGTCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGct                                                                                      <  2:76147/250‑1 (MQ=255)                                                                                                                                                       cGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGTCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGct                                                                                      >  1:76147/1‑250 (MQ=255)                                                                                                                                                         gTCTGGAAGTTAAACGTGCACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATGGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACACGCGAAGGGTGGGCGATCCCGGCGGCTCCTGACATTGCATTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGATGAAGATCTTTTTGATGGCTCTg                                                                                   >  1:464891/1‑251 (MQ=255)                                                                                                                                                             ggAAGATAAACGTTAACTGATGCAAGGATCGCTAGCCAGCGTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTGTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGc                                                                                 <  1:63286/249‑1 (MQ=255)                                                                                                                                                               aaGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGGAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCtctt                                                                             >  2:225729/1‑248 (MQ=255)                                                                                                                                                                   tAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATcg                                                                         >  1:605389/1‑251 (MQ=255)                                                                                                                                                                        gTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCt                                                                                                         <  1:505384/214‑1 (MQ=255)                                                                                                                                                                        gTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCt                                                                                                         >  2:505384/1‑214 (MQ=255)                                                                                                                                                                          gAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATc                                                                   <  2:470273/250‑1 (MQ=255)                                                                                                                                                                                 tGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGGGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGCTCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGcc                                                           >  1:331329/1‑251 (MQ=255)                                                                                                                                                                                    aaGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCcatc                                                        >  2:243645/1‑251 (MQ=255)                                                                                                                                                                                     aGGATCGCTAGCCAGCTTACGCCAGGTCGCATTTCCAGTTATCGCCTCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGTCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGAGCGATCCCGGCGTCTACTGACATTGCCTTTGCACTTGGTGTGCTGTCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGACCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCcatca                                                       <  1:291562/251‑1 (MQ=255)                                                                                                                                                                                      ggATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGCCATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGACTATTATCGACGATCTTGGGGAcatcat                                                      >  1:332984/1‑251 (MQ=255)                                                                                                                                                                                              aGCCTGCTTACGCCAGGCCGCATTTCCAGTTTTCGCCGCTATTGGTGGGATGATTGTTCCGGCATTTCGCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTATTGACATTGCCTTTGCTCTTGGTGTGCTGGCGCTGTTGGGAAGTCTTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCTACGATCTTGGGGCCATCATTatcatc                                               <  2:443913/250‑1 (MQ=255)                                                                                                                                                                                               gCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTGTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATTGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGc                                             <  1:312838/251‑1 (MQ=255)                                                                                                                                                                                                   gCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGACGGGTGGGCGATCCCGGCGGCTCCTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTg                                         >  1:83457/1‑251 (MQ=255)                                                                                                                                                                                                     ttACGCCAGGCCGCATTTCCAGATATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTACCTTTGCACTTGGTGTGCTGGCTCTGTTGGGAAGTCGTGTTCCGTTCGCGCTGAATCTCTTTTTGATTGCTCTGGCTATTATCGACGATCTTGGGGCCCTCCTTCTCCTCGCATTGtt                                       >  2:462794/1‑251 (MQ=255)                                                                                                                                                                                                                cGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGCTTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAAtt                            >  1:452484/1‑250 (MQ=255)                                                                                                                                                                                                                  cATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGAc                          <  1:555463/251‑1 (MQ=255)                                                                                                                                                                                                                    tttCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTg                                                                                                                                         >  1:281938/1‑138 (MQ=255)                                                                                                                                                                                                                    tttCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTg                                                                                                                                         <  2:281938/138‑1 (MQ=255)                                                                                                                                                                                                                     ttCCAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACtt                        <  2:158800/250‑1 (MQ=255)                                                                                                                                                                                                                       ccAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATc                     >  2:257721/1‑251 (MQ=255)                                                                                                                                                                                                                        cAGTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATACCGGCGGATACTGACATTGCCTTTGCACTTGGTGTGCTGGAGCTGTTGGGAAGTCGTGTTCCGTTAGCGATGAAGATCTTTTTGATGGCTCTGGCTATTATAGACGATCTTGGGGCAATCATAATCATCGCATTGTTCTACCCTAATGACTTAt                      >  1:582769/1‑249 (MQ=255)                                                                                                                                                                                                                          gTTATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGAt                  <  1:438784/251‑1 (MQ=255)                                                                                                                                                                                                                            tATCGCCGCTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATgg                >  2:218369/1‑251 (MQ=255)                                                                                                                                                                                                                              tcgacgcTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCAGATCAGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGCTGGCTCTGGCTATTATCGACGATCTTGGGGCCATAATTATCATCGAATTGTTCTACACTAATGACTTATCGATGGcc              >  2:1493/5‑251 (MQ=255)                                                                                                                                                                                                                                gccgcTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCg                                              <  1:439524/217‑1 (MQ=255)                                                                                                                                                                                                                                gccgcTATTGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCg                                              >  2:439524/1‑217 (MQ=255)                                                                                                                                                                                                                                gccgcTATTGGTGGGATGATTGATCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCctc            >  1:176829/1‑251 (MQ=255)                                                                                                                                                                                                                                       ttGGTGGGATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGc     >  2:269065/1‑251 (MQ=255)                                                                                                                                                                                                                                          gttggATGATTGTTCCGGCATTACTCTATCTGGCCTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCCTTTGCACTTGGTGTGCTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTc  >  1:57963/4‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                              GATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTGTTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAACGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCCGCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCGATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTACTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCTATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCGATGGCCTCTCTTGGCGTC  >  NC_000913/17563‑18046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑