Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	17,399	A→G	intergenic (+389/‑90)	sokC → / → nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	17,399	0	A	G	100.0%	111.0 / NA	34	intergenic (+389/‑90)	sokC/nhaA	small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/16);  total (18/16)

TTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGG  >  NC_000913/17161‑17636                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              ttAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTac                                                                                                                                                                                                                                     <  2:254263/250‑1 (MQ=255)               taTCGCAATTATTGACGACAAGATGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGAttt                                                                                                                                                                                                                      >  1:491399/2‑251 (MQ=255)                aTCGCAATTAATGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGAttt                                                                                                                                                                                                                      <  2:216223/250‑1 (MQ=255)                     aaTTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACgt                                                                                                                                                                                                                >  2:347926/1‑250 (MQ=255)                        tattGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAAAGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACgatg                                                                                                                                                                                                              >  1:78231/1‑250 (MQ=255)                         attGACGACAAGCTGGATTATTTTTGAAATATTGTCCTATCAAATAACGCCGACTGACAAGAAATTTATTATTACTTTTCCTAATGAATCACTCAGAAATCCTCACCTTAAGCTATGATTATCTGGACTTAGGTTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGACTCAAATTCTTCAATTTATCGTAAAAAACGAGTTATTCCTCCACTATAATCTGATTTTAACgatgat                                                                                                                                                                                                            <  2:342178/251‑1 (MQ=255)                                  acgCTGGCTTATTTTTGAAATATAGACCTACCACTTAAAGCCGCCTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCATCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGACACTCGTGAGCGCTTACAGCCGT‑CACAAAC‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAACAAACGCGTTATTCCTACACTATAATCTGATTTTAACgatgctttgtgaggg                                                                                                                                                                                                   >  2:570014/3‑240 (MQ=255)                                             tttttGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGt                                                                                                                                                                                         >  2:420499/1‑250 (MQ=255)                                                  gAAATATTGTCCTAACAAATAACGCCGAATGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTaaaat                                                                                                                                                                                    <  2:205211/250‑2 (MQ=255)                                                     atatTGTCCTAACAAATAACGCCGACTGACAAGCAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGAt                                                                                                                                                                                >  1:497876/1‑251 (MQ=255)                                                                    ataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGaa                                                                                                                                                                   >  1:39926/5‑249 (MQ=255)                                                                    ataacGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCACTCAGAAATCCTCACCTTAAGCTCTGTTTATCTCGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGCTTTGCGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGaa                                                                                                                                                                   >  1:414704/5‑249 (MQ=255)                                                                            gACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATaa                                                                                                                                                         <  1:380938/251‑1 (MQ=255)                                                                                   aagaaaTTAATTATTTCTTTTCCTAATTAATCCCTCAGAAAGCCGCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGA‑CA‑AAAAACTCATCTCTCCGCTGATTGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACATTATAAGCTGATTTTAACGATGATTTGGGCGGTGTAAAATAGTAAAAACGATCTAGTCGCCTGAAAGAGAAAGAAAAAGTGa                                                                                                                                                  <  2:564695/248‑1 (MQ=255)                                                                                     gaaaTTAATTATTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGaa                                                                                                                                                 >  1:408485/2‑250 (MQ=255)                                                                                           aattaTTACTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCt                                                                                                                                           <  1:624850/250‑1 (MQ=255)                                                                                                 taCTTTTCCTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCg                                                                                                                                     >  1:386136/1‑250 (MQ=255)                                                                                                        ccTAATGAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCATAGTGTCAATCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGGGCGGGGTAAAATAGTAAAAACGATCGATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGTTTCTTTa                                                                                                                             <  2:51532/251‑1 (MQ=255)                                                                                                         cTAATTAATCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCttt                                                                                                                              >  2:160993/1‑249 (MQ=255)                                                                                                              taatCCCTCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTg                                                                                                                       >  2:6187/1‑251 (MQ=255)                                                                                                                     tCAGAAATCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTc                                                                                                                >  2:374203/1‑251 (MQ=255)                                                                                                                      cagaaaTCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGTCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACTCTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATATTAAAAACGATCTATTCCCCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTc                                                                                                                <  2:554746/246‑1 (MQ=255)                                                                                                                      cagaaaTCCTCACCTTAAGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCg                                                                                                               >  1:141432/5‑251 (MQ=255)                                                                                                                                     gaaGCTATGATTATCTGGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCTGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCtt                                                                                                <  1:595755/250‑1 (MQ=255)                                                                                                                                                       gCTTAGGGTCACTCGTGAGCGCTTACAGCCGTTAA‑AAAA‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTg                                                                              <  1:41290/251‑1 (MQ=255)                                                                                                                                                                 aCTCGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATACCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGAtt                                                                     >  1:443913/1‑250 (MQ=255)                                                                                                                                                                    cGTGAGCGCTTACAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTAt                                                                   <  1:428952/249‑1 (MQ=255)                                                                                                                                                                               aCAGCCGT‑CA‑AAAAACTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATGTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGTGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAgcggc                                                      <  2:291636/251‑1 (MQ=255)                                                                                                                                                                                           aaaa‑CTCATCTCACCGCTGATGGCGCAAATTCTTCAATGGATCGTAAAAAACGCGTTATTCCTACACTATAATCTGATTTTAACGATGCTTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGACATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTgg                                           >  2:612077/1‑251 (MQ=255)                                                                                                                                                                                                        aCCGCTGATGGCGCAAATTCTTCAATGGCTCGTCAAAAACGAGTTGTTCGTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGGCAAATAGTAACAACGATCTATTCACCTGAAAGAGAAATACAAAATGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCCACCAGTGGATGGTATCACgc                               >  2:34682/1‑250 (MQ=255)                                                                                                                                                                                                                      aaaTTCTTCAATGGATCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACATGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGGGACGc                 >  1:510549/1‑251 (MQ=255)                                                                                                                                                                                                                                  ggatCGTAAAAAACGAGTTAATCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTAGTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGAGTCTTTAGCAGTGATGCCTCGGGAGGCAGTATTCTTATCATTGCCGCTATCCTGGCGATGATGATGTCCAATAGCGGCGCAACCAGTGTATGGTATTACGTCTTTCTGGATACGcgcgtgcagctc      <  2:95923/247‑12 (MQ=255)                                                                                                                                                                                                                                    atCGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCgg   <  1:613947/250‑1 (MQ=255)                                                                                                                                                                                                                                      cGTAAAAAACGAGTTATTCCTACACTATAATCTGATTTTAACGATGATTTGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAATAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCggg  <  2:386136/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                              TTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGAATCCTCACCTTAAGCTATGATTATCTAGGCTTAGGGTCACTCGTGAGCGCTTACAGCCGT‑CA‑AAAA‑CGCATCTCACCGCTGATGGCGCAAATTCTTCAATAGCTCGTAAAAAACGAATTATTCCTACACTATAATCTGATTTTAACGATGATTCGTGCGGGGTAAAATAGTAAAAACGATCTATTCACCTGAAAGAGAAATAAAAAGTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATTGCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGACTTTCTGGAGACGCCGGTTCAGCTCCGGG  >  NC_000913/17161‑17636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑