Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 17,039 C→T intergenic (+29/‑450) sokC → / → nhaA small regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091317,0390CT90.0% 48.6 / ‑5.0 21intergenic (+29/‑450)sokC/nhaAsmall regulatory RNA antitoxin SokC/Na(+):H(+) antiporter NhaA
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base T (16/2);  minor base A (1/1);  total (17/4)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGA  >  NC_000913/16811‑17282
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
cTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGCTACAGCTGACGATAAGGGCGCCAATCATCGCCTTATGCTGATTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCCGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCAATAGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCtct                                                                                                                                                                                                                                >  1:388717/1‑249 (MQ=255)
               tctcGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGGAAATCATCGCCTTAAGCTGCTTCAATGC‑TCTCTTCTCCTTGACCTTACGGACAGCAAGAGGCACCCTACATGTGCTGAGCATATAGGGGGACTCGGGATGATAGGAACATATCACTCTGGGACTTTC‑TCTATCTGCCGTTCCGGTAATGCCTGAGACATATAGCCTCCAGCACCCGCCGCTATTATCTCgtttttttt                                                                                                                                                                                                                 >  1:608440/1‑243 (MQ=255)
                     tACCAGCGCCGCCACTACGGCGGTGATAAAGATGAAGAACAGGGAGACAAACAACGCCTTATTCTGCTTCAATGA‑TCTCTTCTCCTTTACCCTACGGTCAGTAAAAGGCACTCTACATGTGGTCCGCCTAAATGGGGCCTCGGGTTGGTTGTAAAATATCACTCAGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGAAAGACTCAATCACCCGCCGCTATTATATCTCTCCCCTTTAAccc                                                                                                                                                                                                           >  2:445256/1‑251 (MQ=255)
                                                   gATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTCTCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGAc                                                                                                                                                                            >  1:254263/1‑251 (MQ=255)
                                                    aTGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGA‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGATGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTTAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCCTTCTGTTTTATCGATTCTAATCCTgtcgacg                                                                                                                                                                           >  2:476368/1‑245 (MQ=255)
                                                           tCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGc                                                                                                                                                                     >  1:216223/1‑250 (MQ=255)
                                                                 cGACAAACACCGCTTTATGCTGCTTC‑TTGCCTCTTTTCCCCTTGCCCTTACGGTAAGAAAGAGGCACTCTACATGTGTTCAGCATAAGGGGGGCCGGGGTTTGAAAATAAAATATCCCCGGGGGCTTTTC‑TTTTTCTGCCGTTCAGCTAAGGCTGGAGACATATAGCCTCAACCCCCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTtg                                                                                                                                                              <  1:263560/251‑1 (MQ=255)
                                                                  gACAATCATCGCCTTATGCTGCTTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTtgt                                                                                                                                                             >  1:591731/1‑251 (MQ=255)
                                                                                 atcctgctTCTTTTC‑TCCCTTCCCCTTGCCCTTGGGGTAGGTAAGAGCCACTCTACTTGTTTTCAGCATAGAGGGGGCCTCGGGTGAATAAAAAAATATCAC‑CGGGGGTTTTCCTTTCCCTCCCGTTCATTTAATGCCTGAAACAGACAGCCTAATGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTaa                                                                                                                                              <  2:431476/248‑1 (MQ=255)
                                                                                   gctgctTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATg                                                                                                                                            >  1:205211/1‑251 (MQ=255)
                                                                                     gctttcttttc‑tctctTCTCCTTGTCTTTACGGTAATTAAGAGCCCCTCTACATTTGTCCAGCATATGGGGGCCCTGGGGTTAATAGTAAAATATCCCTCGGGTTTTTTC‑TCTATCTGCGGTTCACCTAATCCCGGAGACAGATACCCACAAGCATCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGAt                                                                                                                                          <  1:13536/241‑1 (MQ=255)
                                                                                                            tGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAAttt                                                                                                                     >  1:209526/1‑250 (MQ=255)
                                                                                                              aCCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATCTAtc                                                                                                                  >  1:5222/1‑251 (MQ=255)
                                                                                                                     ggTCAGTAAGAGGCACTCTACTTGTGTTCGGAATAAAGGGGGCCGCGGTTTGAAAGTAAAATTTCCCGCGGGGTTTTTT‑TCTATCTGCCGTGCAGCTAAGGCCGGAAACAGAAACCCACAATCGTCCGCCGCTATTATATCGCTCTC‑TTTAACCCAGTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTAAATTATTTAACTTTAATTTATCTCtttat                                                                                                           <  1:269246/251‑4 (MQ=255)
                                                                                                                            aaGAGGCACTCTACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAatt                                                                                                    >  1:291636/1‑251 (MQ=255)
                                                                                                                                       tACATGTGTTCAGCATATAGGGGGCCTCGGGTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACa                                                                                          >  1:51532/1‑250 (MQ=255)
                                                                                                                                                                 tCGGGTTGATAGTAAAATATCCCTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGCTTATTTTTGAAATCTTgtcc                                                               >  2:124606/1‑248 (MQ=255)
                                                                                                                                                                    ggTTGATAGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTa                                                             >  1:24580/1‑250 (MQ=255)
                                                                                                                                                                                   atCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTCTTATATCGCTCTA‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACt                                             >  2:624850/1‑251 (MQ=255)
                                                                                                                                                                                                                ttCAGCTAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCt                  >  1:342360/1‑250 (MQ=255)
                                                                                                                                                                                                                      tAATGCCTGAGACAGATAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTACTCCTGAAGACGACTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGTCAAGCTGGATTATTTTTGAAATATTGTCATAACAAATAACGCCGACTGACAAGAAATTACTTATTACTTTTCCtaattaat           >  1:95923/1‑251 (MQ=255)
                                                                                                                                                                                                                                gacagataGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTCTGTTTTATCGATTCTAATCCTGACGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTTATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGTCCTAACAAATAACGCCGACTGACAAGAAATTAATTATTACTTTTCCTAATTAATCCCTCAgaa  >  2:531747/1‑248 (MQ=255)
                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    
CTCACAGAGGTCTTTTCTCGTTACCAGCGCCGCCACTACGGCGGTGATACAGATGACGATCAGGGCGACAATCATCGCCTTATGCTGCTTCATTGC‑TCTCTTCTCCTTGACCTTACGGTCAGTAAGAGGCACTCTACATGTGTTCAGCATATAGGAGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTC‑TCTATCTGCCGTTCAGCTAATGCCTGAGACAGACAGCCTCAAGCACCCGCCGCTATTATATCGCTCTC‑TTTAACCCATTTTGTTTTATCGATTCTAATCCTGAAGACGCCTCGCATTTTTGTGGCGTAATTTTTTAATGATTTAATTATTTAACTTTAATTTATCTCTTCATCGCAATTATTGACGACAAGCTGGATTATTTTTGAAATATTGGCCTAACAAGCATCGCCGACTGACAACAAATTAATTATTACTTTTCCTAATTAATCCCTCAGGA  >  NC_000913/16811‑17282

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑