| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,593 | A→G | A419A (GCA→GCG) | thrA → | fused aspartate kinase/homoserine dehydrogenase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,593 | 0 | A | G | 99.0% | 280.2 / NA | 104 | A419A (GCA→GCG) | thrA | fused aspartate kinase/homoserine dehydrogenase 1 |
| Reads supporting (aligned to +/- strand): ref base A (1/0); major base G (48/54); minor base T (1/0); total (50/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
GATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCT > NC_000913/1350‑1837 | gATGTCCCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGccc < 2:434441/251‑1 (MQ=255) gTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCcgcg < 1:298211/251‑1 (MQ=255) cgcgCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGAGCACCTTGCGGGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCa > 1:259491/1‑250 (MQ=255) cccGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCACTGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGGAGGCGCTGGTTTTCGCCCCTTGCGTGGGATCCCGGGCAAATTCTTTGCCGCGCTGGACCGCGCCAATATc > 2:210401/1‑251 (MQ=255) cGTATTTCCTTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAGCTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑aa < 1:306809/251‑1 (MQ=255) cGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGGGTGGGATCTCGGCGAAATTTTTTGCCGCGCTGGCCCGCGCCAATATC‑a > 2:616708/1‑250 (MQ=255) aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGACCGGAA‑GGCTGGCCATTATCTCGGGGGTGGGTGATGGAATGCGGACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCCATATC‑AACA‑t > 2:213410/1‑251 (MQ=255) aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAA‑GGAAAGGCTGGCCATTATCTCGGTGGGCGGGGATGGTCTGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCTCTGGCCCGCGCCAATATC‑AACA‑t > 2:475688/1‑251 (MQ=255) tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑tt > 1:490747/1‑251 (MQ=255) ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGt < 1:483405/250‑1 (MQ=255) agcttatttctcAATCATCCTCCGAATTCAGTATAAGTGTCTTGGTTCCGCAAAGCTACTGTGTGCTCGCTGATCGGGCAATGCAGGAAGAGTTTTACCCGAAACTGAATGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGGTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGTCAATTTC‑AATA‑TTGTCGctagt < 2:353887/240‑5 (MQ=255) gATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCACCTTGCGAGGGTTCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑ACCA‑TTGTCGCCATTGCTc > 2:512921/1‑251 (MQ=255) cGCAATCATCTTCCGAATACAGTAGCAGTTTCTGCGTTCCGCAAAGCGACTGGGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTGACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGTGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGa < 2:576449/251‑1 (MQ=255) gCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGGACGGCTGGCCATTATCTCGGTGGTAGGTGGTGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATACC‑AACA‑TTGTCGCCATTGCTCAGGGAt > 2:607770/1‑251 (MQ=255) cAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGGATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAggggtc > 2:109474/1‑248 (MQ=255) tcatcTTCCGAATACAGTATAAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTTCGGGAtcttc > 2:107886/1‑251 (MQ=255) atcatctgcaatcagtcacAGTTGCTGCGGTCCGCAAAGCGACTGGGGGCGAGCTGATCGGGCAATGCAGGAAGAGTGCGACCTGGAACTGAAAGAAGGCTTACTGGAGTCGC‑TGGCGGTGAC‑GGAACGTCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCCTGCGTGGGTTCTCGGCGAATTTCTTTGCCGCGCTGGCCCGCGCCACTAGC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTg < 2:371223/233‑1 (MQ=255) tcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTg > 1:87486/1‑250 (MQ=255) tcTTCCGAATACAGTATCAGATTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGTAGGTTTGCGCACCTTGCGGGGGATCTCGGCGAAATTCTTTGCCGCAGTGGGCCTCGCCAATATC‑AACA‑TTGTACCAATTTCACAGGGAtcttct > 2:454678/1‑249 (MQ=255) tCCGAATACAGTATCAGTTTCTTCGTTCCGCAAAGCGACTGTGTGCGATCTGAACGGGCAATGCAGGAAGAGTTCTTCCTGTAACTGAAAGAATGCTTACGGGAGGCGC‑TGGCGTTGAC‑GGAACGGCTGGCCATTATCGCGGGGGTAGGTGATGGTAGGCGCACCTGGCGTGGGATCTCGTCGAGATTCTTTGCCGCGCTTGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAAc < 1:316492/250‑1 (MQ=255) aTCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGGTGGTATACGCACCTTGCGGGGGATCTCGGCGAAATTCTTTTCCGCGCTGGCCCGCGCCAATATCCAAC‑‑TTGTCGCAATTGCTCAGGGATCTTCTGAACGCTCaacctattt > 2:142546/1‑244 (MQ=255) aTCAGTTGCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGt < 2:267049/251‑1 (MQ=255) cTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGTTCTTCTGACCGCTCCATCTCTGT‑CGTGGTa > 1:485518/1‑250 (MQ=255) cgttacgcaAAGCGACTGTGTGCGAGCTGAACGGGCGATGCAGGAAGAGTTCTACCGGGAACGGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGAGCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATa < 2:478149/244‑1 (MQ=255) ttCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAAc > 1:488491/1‑251 (MQ=255) tccgcaAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGCACGCTCAATCTCTGT‑CGTGGTAAATAACg > 1:291082/5‑251 (MQ=255) aaaGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGGGGTAAATAACgatgat > 1:253763/1‑251 (MQ=255) aGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTTT‑CGTGGTAAATAACGATGATGc < 1:113115/251‑1 (MQ=255) aGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACGGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGc < 2:231809/251‑1 (MQ=255) gACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGCC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAACTTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGAc > 1:296407/1‑251 (MQ=255) aCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGTACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGCC‑GGCCCGGCTGGCCATTATCTCGGTGGTAGGTGATGGTTTGAGCACCTTGCGTGGGATTTCGGCGATATTCTTTGCCGCGCTGGCCCGCGCCAATTTC‑AACA‑TTGTCGCCATTGCTTCGGGTTCTTCTGCACGCTCAATCTCTGT‑CGTGGTAAATAACGTTGATGCGAcc > 2:326673/1‑251 (MQ=255) aCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCCTTTTCTCGGTGGTAGGGGATGGGATGAGGACAATGCGTGGGAACTCGGCGAAATTCATTGACGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTTTGCACGCTCACTCTTTGG‑CGTGGCAAATAACGATGATGCgccc > 2:545654/1‑248 (MQ=255) gAGCTGAACGGGCAATGCAGGAATAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCTTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCTCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑Tg < 1:576476/251‑1 (MQ=255) aGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑Tgc < 1:185186/251‑1 (MQ=255) cTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑Tgcgc > 1:475509/1‑251 (MQ=255) gAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGt > 1:133861/1‑251 (MQ=255) cGGGCAATGCAGGAAGAGTTCTGCCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTAc < 1:431895/251‑1 (MQ=255) gggCAAGGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TGGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCGCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACt < 2:384469/251‑1 (MQ=255) aTGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCAGTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACtcatca < 1:616708/251‑1 (MQ=255) gCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAg < 1:294235/250‑1 (MQ=255) gCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAAAGATGATGCGAACACTGGCG‑TGCGCGTTACtcatc > 1:486228/1‑248 (MQ=255) cAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATATCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCCGAt > 1:265079/1‑251 (MQ=255) cAGGAAGAGTTCTACCTGGAACGGTAAGAAGGCTTTCTGGAGCCGC‑TGGCGGTGAC‑GGAGCGGCTGGCCATTATCACGGGGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCGGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGAGCGTCTGAACGCTCAATCGCTGT‑CGTGGTAAATATCGATGATGCGACCACGGGCG‑AGCGCGTTACTCATCAGAt < 2:273300/251‑1 (MQ=255) aGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATg > 1:125366/1‑251 (MQ=255) aggtCTACCTGGAACTGAAAGAAGGGTGACGGGAGCCGCGTGG‑GGTGAC‑GGGACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCGCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCGCAGGGATCGTCGGAACGCTCAATCTCTGT‑CGTGGTAAATAACTATGATTCGACCACTGGCG‑TGCGCGTGACTGATCAGATGCTGTTc < 2:65395/248‑1 (MQ=255) gccTGGAATTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATAcc < 2:396849/250‑1 (MQ=255) aacTGGAACTGAAAGAAGGCTTACGGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTGCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑GGCGCGTTACTCATCAGATGCTGTTCAAt < 2:291082/246‑1 (MQ=255) aCCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATAc < 1:529252/250‑1 (MQ=255) aCCTGGAACGGAAAGAAGGCTTTCTGGAGCCTC‑TGGCGGTTAC‑GGAACGGCTGGCCATTATCTCGTTGGTAGGTGATGGTATTCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTTGCCCGCGCTAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAAt < 1:480550/248‑1 (MQ=255) gAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGGAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTTTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATATCTGT‑CGTGGTAAATAACGATGATGGGCCAACTGGCG‑TGCGCGGTAATACTCAGATGCGTTTCAATCCCGATc > 2:509294/1‑250 (MQ=255) aaCTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATc > 1:335477/1‑249 (MQ=255) gAAAGAAGGCGTACTTGAGCCGC‑TGGAGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATAGCGATCAGGtt < 2:259491/250‑1 (MQ=255) aaaGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTAt < 1:224192/251‑1 (MQ=255) ggCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAgg > 1:512940/1‑250 (MQ=255) cTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAgtgt > 1:53714/1‑251 (MQ=255) tgcTGGAGCCGC‑TGGCGGTGGC‑GGGACGGTTGGCCACTATCTCGGGGGTAGGTGATGGTATGCGCGCCGTGCGTGGGATCTCGGCGCAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGTTCTTCTGAACGCTCAATCGCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGCTTATCGAAgtgt < 2:75706/247‑1 (MQ=255) gtattcGC‑TGGCGGTGAC‑GGTACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCGGAATGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCTTTACTCATCAGATGCTGTTCAATACCGCTCAGGTTATCGAAGTGTTtgt < 1:44995/244‑1 (MQ=255) ggAGCCGC‑TGGCGGTGAC‑GGAACGGGGGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCTCCGTGCGTGGGATCGCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTC‑GTGCGTGGTAAATAACGAGGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGGTCAATACCGATCAGGTTCGCGAATTGTATGTGa < 2:562241/251‑1 (MQ=255) gAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTCTGTGAt < 1:215259/251‑1 (MQ=255) ccGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGGTATCGAAGTGTTTGTGATTgg > 1:527310/1‑251 (MQ=255) cGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGGAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGc > 2:448544/1‑251 (MQ=255) c‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTCGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGt > 2:79192/1‑251 (MQ=255) ggcggTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCg > 1:408979/5‑251 (MQ=255) ggcggTGAC‑GGAACGGCTGGCCAGGATCTCGGTGGGATGTGATGGTATGCGCCCCTTGCGTGGGATCCCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGACATTGCTCAGGGATCTTCTGAACTCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGTCG‑TGCGCGTTACTCATCAGATGCTTTTCAATACCGAGCATGTTATCGAAGTGGTTGTGATTGGCGTc < 2:572932/246‑1 (MQ=255) ggcggTGAA‑GGAACGGCTGGCCAGTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGt < 2:100579/245‑1 (MQ=255) gggtgtc‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGt < 2:488491/245‑1 (MQ=255) gTGAC‑GGAACGTCGGGCGCTTATCTCGGTGGTAGGTGATGGTATGCGCCCCTTGCGTGGGATCTGGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCTATTGCTCAGGGATCTTCTGAACGCGCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCGCTGGTG‑TGCGCGTTTCTCATCAGATGCTGTTCAATACCGGTCAGGTTATCTAAGGGTTTGTGATTGGCGTCGGTgg < 2:53861/251‑1 (MQ=255) gAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGGGTTTGTGATTGGCGTCGGTGGc < 1:121099/250‑1 (MQ=255) ggAACGGCTGGCCATTATCTCGGTGGTAGGTGTTGGTATGCGCACCTTGCGTGGGATCTCGGAGAAATTCTTTCCCGCGCTGGCCCTCGCCAATATA‑AACA‑TTGTCGCCATTGCTCCTGGATCTTCTGAACGCTCCATCTCTGT‑CGAGGTAAATAACGATGATGCGCCCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGCTCAGGTTATCGCAGTGTTTGTGATTAGCGTCGGTGGCGTTg > 2:154687/1‑251 (MQ=255) ggAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTg < 2:490747/251‑1 (MQ=255) ggCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGt < 1:472822/251‑1 (MQ=255) ggCTGGCCATTATCTCGGTGGTAGGTGATGCTATGAGCACCTAGCGTGGGATCTCGGCGAAATTCTTTGACGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTAAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGGTG‑GCGTTAATCATCAGATGCTGTTCAATACCGATCAGGTTATCGCAGTGGTTGGGATTGGCGTCGGGGGCGTTGGCggg > 2:364096/1‑250 (MQ=255) gCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGCTCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTg > 1:527792/1‑251 (MQ=255) gCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGGTTGTGATTGGCGTCGGTTGCGTTGGCGGTGCgct < 1:190878/251‑1 (MQ=255) tATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGAGCCAATATC‑AACA‑TTGTCGACATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTACATAACGATGATGCGAACACTGGCG‑TGCGCGGTACTCATCAGATGCTGTTCAATACCGATAAGGTTATCGAAGTGTTTGGGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTgg > 1:30299/1‑251 (MQ=255) tcGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGGTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCg > 1:125639/1‑235 (MQ=255) ggtggtAGGTGTTGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGCAGGGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAAc > 2:518264/1‑251 (MQ=255) ggaggttggtactgcaaagtgcACCTTACGTGGGATCTCGGAGAAATTCTTTGCCGCGCTGGCACGCGCCAATATC‑AAGA‑TTGTCGCGATTGCTCAGGGATCTTCTGAACGATCAATCTATGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCCATACCGATCCGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAAc > 2:251784/21‑251 (MQ=255) ggtAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTg < 1:482308/250‑1 (MQ=255) ggtAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTg > 2:260401/1‑250 (MQ=255) gTGATGGTATGCGCCCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGt < 2:19880/251‑1 (MQ=255) aTGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCAGTGCTCAGGGATCTTCTGAACGCTCAATCGCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑GGCGCGTTACGCATCATATGCTGTTCAAGACCGATCAGGTTATCGAAGTGTTTGTGATGGTCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaa < 1:599606/251‑2 (MQ=255) ggTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCCGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaac > 2:599251/1‑248 (MQ=255) gTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCaaca > 1:599512/1‑247 (MQ=255) gTATGCGCACATTGCGTAGGCTCTCGGCGAACTTCTTTGCCGCGCCGTCCCGCGCCAAGATC‑CACC‑TTGTCGCCATTGCTCAGGGACCTTCTGAGCGATCAATCTCCGT‑CGTAGTAAATAACGATGATGCTACCACTGGCG‑TGCGCGTTACTAATCAGATGCTGTTCAATGCCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTTGTCTGCTGGGACAACTGCAGCGtagaca > 2:278152/1‑245 (MQ=255) gcACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAAAAAAgctgg > 2:495710/1‑251 (MQ=255) aCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACGGAAGCGTCAACAAAgctggct < 2:311910/251‑1 (MQ=255) tGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTga < 2:475509/249‑1 (MQ=255) cGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCCACAAAGCTGGCTgaaga > 2:469353/1‑250 (MQ=255) ggggATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGAGGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGAGCAGGTTATCGAAGTGTTGGTGATGGGCGTCGTTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATa < 2:253763/250‑1 (MQ=255) cGGCGAAATTCTTTGCCGCGCTTGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACat < 2:335477/248‑1 (MQ=255) cGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATc < 1:35643/251‑1 (MQ=255) cGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTGCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAAc < 1:213410/246‑1 (MQ=255) gCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGa < 2:172979/251‑1 (MQ=255) aaTTCTTTGCCGCGCTGCCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGaatt > 2:284524/1‑247 (MQ=255) aTTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTAc < 1:175262/251‑1 (MQ=255) tCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACgt > 1:567353/1‑251 (MQ=255) ttGCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCTGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACgtgt < 1:607770/250‑1 (MQ=255) gCCGCGCTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCAGTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCt < 2:496727/250‑1 (MQ=255) gCCGCGCTGGACCGCGACAATAGC‑CACAGTTG‑CGCCAATGCACAGGGATCTTCTGAACGCTACATCCGTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑GGCGCGTTACTCTTCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGGTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAACAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCt < 2:334771/250‑1 (MQ=255) | GATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATC‑AACA‑TTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCG‑TGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGTCGGTGGCGTTGGCGGTGCGCTGCTGGAGCAACTGAAGCGTCAGCAAAGCTGGCTGAAGAATAAACATATCGACTTACGTGTCT > NC_000913/1350‑1837 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |