BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	15,073	A→T	L302L (CTA→CTT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	15,073	0	A	T	99.0%	300.9 / NA	101	L302L (CTA→CTT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (46/53); minor base G (0/1); total (47/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 101 total aligned reads displayed.

TGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCC  >  NC_000913/14826‑15317

tGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGGGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTc                                                                                                                                                                                                                                                    >  1:4330/1‑251 (MQ=255)

  gACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCg                                                                                                                                                                                                                                                  <  1:589577/251‑1 (MQ=255)

    cacTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTa                                                                                                                                                                                                                                                <  2:50297/251‑1 (MQ=255)

     acTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTCAGTTCAGGTTCAGGTTAAACAGCACCCGCTTTTCGAGCGTGAAGGCCACAACCTCTATTGCGACGTCCCGCTCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTAACGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCCGCCCGGTAAGCTTTTCCGTAt                                                                                                                                                                                                                                               >  1:102665/1‑251 (MQ=255)

     acTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTAt                                                                                                                                                                                                                                               <  1:382031/251‑1 (MQ=255)

        ggggaCCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGATCAGGGCGCACCGGCAGGCGATCTGTACGGTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTAGTGCGAAGTCCCGATCAACTGCGCTATGGCGGCGCTGTGTGGCTAAATCGAAGTACCGACCCTTGATGGTCGCTTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGGAAGCTTTTGCGTATgtg                                                                                                                                                                                                                                            <  2:298556/248‑3 (MQ=255)

         gagaCCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCCAACTGAAAGTGCCTGGCGAAACCAAGACCGGTAAGCTTTTCCGTATgcgc                                                                                                                                                                                                                                           >  2:438340/1‑251 (MQ=255)

          agaCCGCATCCGGCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGGTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGcgcg                                                                                                                                                                                                                                          <  2:581247/251‑1 (MQ=255)

            aCCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGt                                                                                                                                                                                                                                        <  1:494028/251‑1 (MQ=255)

               gCATCCGTCTTGAGGGCGAAGGAGAAGCGGACGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTaa                                                                                                                                                                                                                                      >  1:511982/1‑250 (MQ=255)

                 aTCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAgg                                                                                                                                                                                                                                   >  1:188289/1‑251 (MQ=255)

                 aTCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAgg                                                                                                                                                                                                                                   >  1:555209/1‑251 (MQ=255)

                 aTCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAgg                                                                                                                                                                                                                                   <  1:626766/251‑1 (MQ=255)

                 aTCCGTCTTGAGGGCGCAGGAGAAGCGGGCGAGCATGGCGCACCGGAAGGCGATATCTACGTTCAGGTACAGGTTAAACCGCACCCGATTTTCGAGCGTGAAGGAAACAACCTATATTGCGAAGTCCCGATCAACTTCGCTAAGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCCGACCGGTCAACTTTTACGTATGCGCGGTAAAgg                                                                                                                                                                                                                                   >  2:586745/1‑251 (MQ=255)

                  tCCGTCTTGCGGGCGAAGGCGAAGCGGACGAGCATGGCGCACCGGCGGGCGATCTGTACGTTCAGGATCAGGTTAAACAGCACCCGATTTTCGAGCGTGGCGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGGCCCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGGAACCCAGACCGGCAAGCTTTTCCGTATGCGCGGGAAAggg                                                                                                                                                                                                                                  >  1:537822/1‑250 (MQ=255)

                   ccGTCGTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCg                                                                                                                                                                                                                                 <  1:373954/251‑1 (MQ=255)

                    cttCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCCGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTCTTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGGTGGTCACGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTCAAGGCGt                                                                                                                                                                                                                                >  1:27754/3‑251 (MQ=255)

                    cGTCTTGCTGGCGAAGGTGAAGCGGGCGATCATGGCGCACCGTCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGTACCCGATTTTCGAGCGTGAAGGCAACAACCGCTATTGCGAAGTCCCGATCATCTTCGCAATGTCGGCGCTGGGTGGCGAAATCTAAGTACCGACCCGTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGt                                                                                                                                                                                                                                <  1:358393/251‑1 (MQ=255)

                     gTCTTGCGGGCGAATGGGAATCGTGCGAGCATGGCGCACCGGCAGGCGCTCTGTTCGTTCAGGTTCAGGTTTAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCTTAATCGAACTACCGTTCCTTGATTGGCGCGTCAAACTGAAAGTGCCTGGCGAATCCCAGACCGGTACGCTTTTCCGTATGCGCGGTAAAGGCGTc                                                                                                                                                                                                                               <  2:263300/251‑1 (MQ=255)

                     gTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTc                                                                                                                                                                                                                               <  1:310430/251‑1 (MQ=255)

                            gggCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTg                                                                                                                                                                                                                        <  1:284517/251‑1 (MQ=255)

                             ggCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGt                                                                                                                                                                                                                       >  1:61617/1‑251 (MQ=255)

                                 aaGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCcgc                                                                                                                                                                                                                   >  1:4021/1‑251 (MQ=255)

                                       aaGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTACAGCCTTTCCGCGGttgc                                                                                                                                                                                                             >  2:81069/1‑248 (MQ=255)

                                            ggCGTGCATGGCGCCCCGGCCGGCGATCTGGACGTTGAGGGTCAGGATAAACAGCACTCGATTTTCGAGCGTGAAGGTAACAACCTCTAGTGCGAAGTCCCGATCAACTTCGCTATGGCGGTGCTGGGTGGAGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTTAAAGTGCCTGGCGAATCCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTTTGTCCGCGGTGGCGcaca                                                                                                                                                                                                        <  2:443444/251‑1 (MQ=255)

                                                aGCATGGCGCACAGGCAGGCGATCTGTACGTTCAGGTACAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGGAATCGCAGTACCGAACCTTGATGGACGCGCCAAACTGAAAGAGCCTGGCGAAACCCCGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGCTGgcgccaagggg                                                                                                                                                                                                    >  2:603409/1‑244 (MQ=255)

                                                     ggCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCCCCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAAGCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAttt                                                                                                                                                                                               <  1:227167/251‑1 (MQ=255)

                                                         cACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCt‑g                                                                                                                                                                                          >  2:556991/1‑251 (MQ=255)

                                                             gggaGGCGATTGGTACGTTCAGGTTCACGTTAAACAGCACCCGATTTTCTAGCGTGAAGGCAACAACCTCGATTGCGAAGTCCCTATCAACTTCGCTCTGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATTGTCGCGTCAAACTGAAAGTGCCTGTCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACCGGGTGATTTGCT‑GTGcc                                                                                                                                                                                      <  2:147706/248‑1 (MQ=255)

                                                             gggaGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGGCCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAGTTGCT‑GTGcc                                                                                                                                                                                      <  2:243603/248‑1 (MQ=255)

                                                             ggCAGGCGGTCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGGGGCGAAATCGCAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGAACGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGACAAGTCTGTCCGCGGTGGCGCACCGGGTGATTTGCT‑GTGcc                                                                                                                                                                                      >  2:70834/1‑251 (MQ=255)

                                                              gCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGAGTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAAGCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTGTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTTCT‑TTGCcg                                                                                                                                                                                     <  2:362259/251‑1 (MQ=255)

                                                                     aTCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGGCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTgtc                                                                                                                                                                              >  2:152779/1‑251 (MQ=255)

                                                                         gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTgtcgtc                                                                                                                                                                           <  1:201137/250‑1 (MQ=255)

                                                                         gTACGTTCAGGTTCAGGTTAAACAGCACCCGATTGTCGAGCGTTAAGGCAACAACCTCTATTGCGAATTCCCGATCACCTGCGATATGGCGGCGCTGGGTGGCGAAAGCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCGGGCGAACCCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGGGTTGCT‑GTGCCGCTTTgtcg                                                                                                                                                                             <  2:537822/248‑1 (MQ=255)

                                                                         atACGTTCAGGTTAGGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCACCAACCGCTATTGCGAAGTCACGATCAATTTCGCTCTGGCGGTGCCGGGTGGCGAAATCGAAGGACCGACCCTTGCTCGTCGCGTCACACTGAAAGGGCCTGGCGAAAGCCAGACCGGTAAGCTTTTCCGTATGCGCGGTTAATGCGTCAAGTCGGTCCGCGGGGGCGCACAGGGGGGTCTGCTCGT‑CCGCGTTGtcgtcg                                                                                                                                                                          >  2:88315/2‑251 (MQ=255)

                                                                                caggttcaggttAAACATCTCCCGATTTTCTATCGTGAAGGCAACAACCTCTATTGAGAAGTCCTGATCAACTTCGCTATGTCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGTCGAAACGCAGACCGGTAAGCTTGTCCGTATGCGCGGTAAAGGCGGCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTTCT‑GTGTCGGGTGGTCGTCGAAactcc                                                                                                                                                                   <  2:287214/251‑4 (MQ=255)

                                                                                 agggtcaggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTTGTGCGAAGTCACGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAGTTGCT‑GTGCCGCGTTGTCGTCGAAACACCg                                                                                                                                                                  <  2:157947/247‑1 (MQ=255)

                                                                                      caggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAgg                                                                                                                                                             <  2:240107/251‑1 (MQ=255)

                                                                                      caggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAgg                                                                                                                                                             <  1:324068/251‑1 (MQ=255)

                                                                                        ggttAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGCAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGCGGCGAAATCGAAGTACCGACCCTTGCTGGGCGCGTCACACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGAAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GCGCCGCGTTGTCGTCGAAACACCGGTAGGcc                                                                                                                                                           >  1:397683/1‑251 (MQ=255)

                                                                                             aaCAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCTACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAATGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAAc                                                                                                                                                      <  1:272846/251‑1 (MQ=255)

                                                                                                     ccGATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaagca                                                                                                                                              <  2:377132/251‑7 (MQ=255)

                                                                                                       gATTTTCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGagaagc                                                                                                                                               >  2:335640/1‑243 (MQ=255)

                                                                                                        agtttCGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCGATGGCGGCGCGGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACTCCGGGAGGCCTGAACGAGAAGCAGaa                                                                                                                                           <  2:576066/249‑1 (MQ=255)

                                                                                                             cGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCCAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAgc                                                                                                                                      >  1:301891/1‑251 (MQ=255)

                                                                                                             cGAGCGTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAgc                                                                                                                                      >  1:503815/1‑251 (MQ=255)

                                                                                                                  gTGAAGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGGGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGGGAAGCAGAAACAGctgctg                                                                                                                                 >  1:198576/1‑251 (MQ=255)

                                                                                                                     aaGGCAACAACCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgca                                                                                                                               >  1:459200/1‑250 (MQ=255)

                                                                                                                           acaacCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaagagctg                                                                                                                        <  2:61617/251‑1 (MQ=255)

                                                                                                                             aacCTCTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaagagctgca                                                                                                                      <  1:622144/251‑1 (MQ=255)

                                                                                                                               cctctATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTgctgcaagagctgca                                                                                                                      >  1:75317/5‑249 (MQ=255)

                                                                                                                                    atggCGAAGGCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGAGGGTCGCGGCAAACTGAAAGTGCCTGGCGAAACCCAGACCTGTAAGCGTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAGTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGGAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGATCTGCAAGaaa                                                                                                                 <  2:400268/246‑1 (MQ=255)

                                                                                                                                    aTTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAg                                                                                                                <  1:458166/250‑1 (MQ=255)

                                                                                                                                       gCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTc                                                                                                            <  1:676/251‑1 (MQ=255)

                                                                                                                                       gCGAAGTCCCGATCAACTGCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCtt                                                                                                             <  1:335640/250‑1 (MQ=255)

                                                                                                                                          aaggcccGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGt                                                                                                         <  2:173316/247‑1 (MQ=255)

                                                                                                                                          aaGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCggg                                                                                                         >  1:604744/1‑250 (MQ=255)

                                                                                                                                          aaGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGGCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCGTCGGt                                                                                                         <  2:443900/251‑1 (MQ=255)

                                                                                                                                            gTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGACGGACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGAGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGGTTTGCT‑GTGCCGCGTTGTCGGCGGAACACCGGTAGGCCTGGACGGGAAGAAGCAACAGCGGCTGCAAGATCTGCAAGAAGACTTCggggg                                                                                                       >  2:148046/1‑248 (MQ=255)

                                                                                                                                               ccGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGccc                                                                                                    >  1:47701/1‑251 (MQ=255)

                                                                                                                                                cGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTCCTGCAAGAGCTGCAAGAAAGCTTCGGTGGccc                                                                                                    >  1:462756/1‑250 (MQ=255)

                                                                                                                                                 gATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGGCGCGTCAAACTGAAAATGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGGGGCCCa                                                                                                   >  1:147750/1‑250 (MQ=255)

                                                                                                                                                 gATCAACTTCGCTATGGCGGCGCTGGGTAGCGAAATAGAAGTACCGAACCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACACAGACCGGAAAGCTTTTCCGTATGCGCGGGAAAGGCGTCAAGTCTATCCGCGAGGGAGCCAAGGGGGCTTTGCT‑GTGCCGCGTGGGAGTCGAAACACCGGTAGGCCTTAAAGAGAAGCTGAAACAGCTGCTGAAAGAGCTGCAAGAACGCttaggaggcaca                                                                                                   >  2:459258/1‑240 (MQ=255)

                                                                                                                                                    cAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAAcc                                                                                                <  1:455957/250‑1 (MQ=255)

                                                                                                                                                        ttCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTg                                                                                                                                                                                                                        <  1:477141/127‑1 (MQ=255)

                                                                                                                                                        ttCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTg                                                                                                                                                                                                                        >  2:477141/1‑127 (MQ=255)

                                                                                                                                                        ttCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCggtg                                                                                            >  1:237356/1‑248 (MQ=255)

                                                                                                                                                              aTGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGcac                                                                                       <  2:510244/249‑1 (MQ=255)

                                                                                                                                                                ggcggcGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGAGAGCacaac                                                                                    >  2:239657/1‑250 (MQ=255)

                                                                                                                                                                  cggcgCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGCAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGAGAGCACAACAGc                                                                                 >  2:249522/1‑251 (MQ=255)

                                                                                                                                                                         gggTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCGTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCgcgc                                                                           <  2:560301/250‑1 (MQ=255)

                                                                                                                                                                          ggTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTc                                                                         <  2:75317/251‑1 (MQ=255)

                                                                                                                                                                           gTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGCACGCGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACACCAGCCCGCGCTc                                                                         >  1:422747/1‑250 (MQ=255)

                                                                                                                                                                           gTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCa                                                                        <  1:81069/251‑1 (MQ=255)

                                                                                                                                                                           gTGGCCAAATCGAAGTCCCGCCCCTTGATCTTCGCGTCAAACTGAACGCGCCTGTCGCACCCCAGCCCCGTAACCTTTTCCGTATGCGCGTTAAAGGCGTCAAGTCTGTCCCCGGTGGCGCCCAGGGTGCTTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGCAGCAGAAACAGCTGCTGCACGAGCTGCAAGACAGCTTCGGTGGCCCAACCGGTTAGCACACCAGCCCGCGCTcc                                                                        >  2:330087/1‑250 (MQ=255)

                                                                                                                                                                            tGGCGAACTCGAAGTACCGACCCTTAATGGTCGCGTCCAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCGCAACAGCCCGCGCTCa                                                                        >  2:578418/1‑250 (MQ=255)

                                                                                                                                                                               cGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAaga                                                                    <  1:92760/251‑1 (MQ=255)

                                                                                                                                                                                   aTCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGctt                                                                <  2:462756/251‑1 (MQ=255)

                                                                                                                                                                                       aaGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCttt                                                            <  1:556991/251‑1 (MQ=255)

                                                                                                                                                                                        aGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTg                                                           >  2:119982/1‑251 (MQ=255)

                                                                                                                                                                                           aCCGACCCTTGATGGTCGCTTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGGCTGGCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGATCACAACAGCCCGCGCTCAAAGAGCTTTTTTGATg                                                        <  2:416850/251‑1 (MQ=255)

                                                                                                                                                                                           aCCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCAGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGAt                                                         <  1:591290/250‑1 (MQ=255)

                                                                                                                                                                                           aCCGACCATTGACGGTCGCGTCAAACTGAAAGTGCCCGGCCAAACCCAAACCGGTACGCTTTTCCGTATGCGCGGTACACGCGTCAAGTCTGTCCGCGGTGGCGCACAGAGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACCCCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACACCCGCCCGCGCTCACAGAGCTTCTTTgctg                                                        >  2:323527/1‑248 (MQ=255)

                                                                                                                                                                                            ccGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATgg                                                       >  1:188528/1‑251 (MQ=255)

                                                                                                                                                                                            ccGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATgg                                                       <  2:133056/251‑1 (MQ=255)

                                                                                                                                                                                                    tGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTgaag                                                <  1:463497/250‑1 (MQ=255)

                                                                                                                                                                                                     gATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTgaaga                                               >  2:566777/1‑250 (MQ=255)

                                                                                                                                                                                                      atgggcgagTCAAACTTAAAGTGCCTGGCGAAACCCAGCCCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGGCAAGTCTGTCCGCGGTGGCCCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTCTGCTGGTGTGaagaag                                             <  2:16646/243‑1 (MQ=255)

                                                                                                                                                                                                           cgcgTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGttttt                                        <  1:285147/251‑1 (MQ=255)

                                                                                                                                                                                                                cAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTgacg                                   >  2:175811/1‑251 (MQ=255)

                                                                                                                                                                                                                      gAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGAGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGCTTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGCAGCCGAAACAGCTGCTGCAAGCGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAACGAGCTTCTTTGCTGGTGTGAAGAAGTTTTTTGACGACCTg                              >  1:259091/1‑250 (MQ=255)

                                                                                                                                                                                                                        aaGTGCCTGGCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGAcc                           <  1:543894/251‑1 (MQ=255)

                                                                                                                                                                                                                        aaGTGCCGGGCGAAACCCAGACCGGTAAGCTTTGCCGTATGCGCGGTAAAGGCGTCAAGTCTGGCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGAcc                           <  2:27754/251‑1 (MQ=255)

                                                                                                                                                                                                                                 gCGAAACCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCt                  <  2:188289/251‑1 (MQ=255)

                                                                                                                                                                                                                                     aaCCCAGACCGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTcccc              >  1:179308/1‑251 (MQ=255)

                                                                                                                                                                                                                                          aGACCGGTAAGATCTTCCGTATACGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAAGGTAATTTGCT‑GTGCCGCGTTGTCGTCGCAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCACGAAAGCTTCGGTGGCCCAACCGGGGAGCACACCAGCCCGCGCTCAAAGAGATTCTTTGATGGAGTGAAGAAGATTTTTGACGACCGGACACGCTAAACTCCCCaaca          >  2:578480/1‑248 (MQ=255)

                                                                                                                                                                                                                                              cGGTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTg     >  2:159834/1‑251 (MQ=255)

                                                                                                                                                                                                                                                gTAAGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGcc   >  1:523059/1‑251 (MQ=255)

                                                                                                                                                                                                                                                  aaGCTTTTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAGAAGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGTGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGccc  <  2:628380/250‑1 (MQ=255)

TGGACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCGGCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGCAACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATCGAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGTAAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGATTTGCT‑GTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTGCTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCAAAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAACCTCCCCAAAAGCCTGCCC  >  NC_000913/14826‑15317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`