Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,509	A→G	A391A (GCA→GCG)	thrA →	fused aspartate kinase/homoserine dehydrogenase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,509	0	A	G	98.9%	301.6 / NA	94	A391A (GCA→GCG)	thrA	fused aspartate kinase/homoserine dehydrogenase 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (50/43);  minor base T (1/0);  total (51/43)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGT  >  NC_000913/1270‑1754                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                         cTGAATAATATGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGTCTTACTGGAGCCGC‑TGGCGGTGAC‑GGGACg                                                                                                                                                                                                                                              >  2:224192/1‑251 (MQ=255)     ataataTGGCAATGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGGGAC‑GGAACGGCTg                                                                                                                                                                                                                                          >  1:267049/1‑251 (MQ=255)               aaTGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGGTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGCGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGGC‑GGAAAGGCGGGCCCTTAtct                                                                                                                                                                                                                                >  2:294235/1‑251 (MQ=255)                 tGTTCAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCg                                                                                                                                                                                                                              >  1:19880/1‑251 (MQ=255)                     cAGCGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGAATTTCCGTGGTGCTGATTACGCAATCATCTTCCGCAACCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGCAAGAAGGCTTAATGGAGACGC‑TGGCGGCGAC‑CGGACGGCTGGcccatttctcggggg                                                                                                                                                                                                                          >  1:572932/1‑238 (MQ=255)                        cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGAGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCggtggt                                                                                                                                                                                                                         >  1:576449/1‑249 (MQ=255)                        cGTTTCCGGCCCGGGGATGAAAGGGATGGTTGGCATGGCGGAGCGCGTGTTTGCAGCGATGTCACGCGCCCGTCTTTCCGTGGCGCTGATTACGCCATCATCTTCCGAATCCAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTCCTGGAGCCGC‑TGGCGGGGAC‑GGAACGGCTGGCCATTCTCTCgggggt                                                                                                                                                                                                                         >  1:562241/1‑249 (MQ=255)                           ttccggcccggtggtGAAAGGGATGGTTGGCATGGCGGCGCGCCTGGTTGCAGCGATGTCACGCGCCCGTCTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGATTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCGGAACGGGCAATGCTGGGAGAGTTCTACATGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAA‑GGAAAGGCTGCCCAATATATCgggtggagggg                                                                                                                                                                                                                    >  2:54758/15‑242 (MQ=255)                               ggcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGAGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCACTCATCTTCCGAATACAGTATCCGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCCGGAAGAGTTCTACCTGGAACTGAAATAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑CGCACTGCTGGCCACTATCTCGGTGGCAGGGGATgg                                                                                                                                                                                                                >  1:12759/4‑251 (MQ=255)                                gcccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGGGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCCTCTTCCGAATACAGGATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCACTGCCGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCTGTGGC‑GGAACGGCCAGCCCATATCTCggtgggtggggaaggg                                                                                                                                                                                                               >  2:60404/3‑240 (MQ=255)                                 cccGGGGATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTa                                                                                                                                                                                                              <  1:218988/250‑1 (MQ=255)                                 cccGGGGATGAAAGGGATGGTTGGCAGGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTGCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTAGCTCGGTGGTAGGGGATGGTa                                                                                                                                                                                                              <  2:592189/250‑1 (MQ=255)                                     gggATGAAAGGGATGGTGGGCATGGCGGCGCGCGTGTTTGCAGCGAGGTCACGCGCTCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTGCTGCCAGGAACTGAAAGAAGGCGTACTGGAGGGGC‑TGGCGGTGAC‑GGAACGGCGGGCCATTAGCTCGGTGGTAGGGGATGGTATgcg                                                                                                                                                                                                          <  2:34342/251‑1 (MQ=255)                                      ggATGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCCTTATCTCGGTGGTAGGTGGTGGTATgcgc                                                                                                                                                                                                         >  1:334771/1‑251 (MQ=255)                                        aTGAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTGGCAGCGATGTCACGTGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCGGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCAc                                                                                                                                                                                                       <  1:596459/251‑1 (MQ=255)                                          gAAAGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCAcc                                                                                                                                                                                                      <  1:56547/250‑1 (MQ=255)                                            aaGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCCTTAtc                                                                                                                                                                                                                                 >  1:221702/1‑221 (MQ=255)                                            aaGGGATGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTAtc                                                                                                                                                                                                                                 <  2:221702/221‑1 (MQ=255)                                            aaGGGATGGTTGGCATGGCGGCCCCCGTGTTTGCAGCGCTGTCACGCGCCCGTCTTTCCGTGGGGCTGATTACGCAATCATCTTCCGACAACAGTATAAGTTTCTGCGTTCCGCAAAGCGACTGTGCGCAAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGCACTGAAAGAAGGCTTACTGGAGCCGC‑GGGCGGTGAA‑GGAACGGGTGGGCATTATTTCGGTGGGAgggggagggatgtgcaccctt                                                                                                                                                                                                   >  2:115366/1‑232 (MQ=255)                                                 aTGGTTGGCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTgg                                                                                                                                                                                              <  1:340212/251‑1 (MQ=255)                                                       ggCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTAGTTCCGTGGTGCTGATTACGCAAACATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGAtctc                                                                                                                                                                                        <  2:68146/251‑1 (MQ=255)                                                        gCATGGCGGCGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTCGGTGATGGTATGCGCCCCTTGCGTGGGAACTCg                                                                                                                                                                                       >  1:392461/1‑251 (MQ=255)                                                            ggcggcGCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCg                                                                                                                                                                                    >  1:190141/1‑250 (MQ=255)                                                              cggcgCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGAGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGaa                                                                                                                                                                                  >  1:206870/1‑250 (MQ=255)                                                              cggcgCGCGTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACGGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGaaa                                                                                                                                                                                 <  1:404214/251‑1 (MQ=255)                                                                 cgcgcgTGTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTc                                                                                                                                                                              <  1:234876/251‑1 (MQ=255)                                                                   ggcgttgttGCAGCTATGTCACGCGCCCGTGATTCCGTTGTGCTGATTACGCAATCATCTTCCGGATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGTAACTGAAAGAAGGCTTACGGGGGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCGCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCtt                                                                                                                                                                            <  2:50983/244‑1 (MQ=255)                                                                    gcgTGTTTGCAGCGCTGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCCAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGGC‑GGAACGGCTGGCCCTTATCTCGGGGGTAGGTGGTGGCAAGCGCCCCTTGCGGGGGGTCTCGGCGACATTCttt                                                                                                                                                                           >  2:599606/1‑251 (MQ=255)                                                                        gtttGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATTCAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCg                                                                                                                                                                       <  2:149596/250‑1 (MQ=255)                                                                        gtttGCAGCGATGTCACGCGCCCGTAGTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCGGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCg                                                                                                                                                                       <  2:38696/250‑1 (MQ=255)                                                                                 gATGTCCCGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGccc                                                                                                                                                              <  2:434441/251‑1 (MQ=255)                                                                                    gTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCcgcg                                                                                                                                                           <  1:298211/251‑1 (MQ=255)                                                                                        cgcgCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGAGCACCTTGCGGGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCa                                                                                                                                                        >  1:259491/1‑250 (MQ=255)                                                                                            cccGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCACTGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGGAGGCGCTGGTTTTCGCCCCTTGCGTGGGATCCCGGGCAAATTCTTTGCCGCGCTGGACCGCGCCAATATc                                                                                                                                                   >  2:210401/1‑251 (MQ=255)                                                                                              cGTATTTCCTTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAGCTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑aa                                                                                                                                                <  1:306809/251‑1 (MQ=255)                                                                                              cGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGGGTGGGATCTCGGCGAAATTTTTTGCCGCGCTGGCCCGCGCCAATATC‑a                                                                                                                                                 >  2:616708/1‑250 (MQ=255)                                                                                                 aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGACCGGAA‑GGCTGGCCATTATCTCGGGGGTGGGTGATGGAATGCGGACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCCATATC‑AACAt                                                                                                                                             >  2:213410/1‑251 (MQ=255)                                                                                                 aTTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAA‑GGAAAGGCTGGCCATTATCTCGGTGGGCGGGGATGGTCTGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCTCTGGCCCGCGCCAATATC‑AACAt                                                                                                                                             >  2:475688/1‑251 (MQ=255)                                                                                                  tttCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACAtt                                                                                                                                            >  1:490747/1‑251 (MQ=255)                                                                                                     ccGTGGTGCTGATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGt                                                                                                                                          <  1:483405/250‑1 (MQ=255)                                                                                                           agcttatttctcAATCATCCTCCGAATTCAGTATAAGTGTCTTGGTTCCGCAAAGCTACTGTGTGCTCGCTGATCGGGCAATGCAGGAAGAGTTTTACCCGAAACTGAATGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGGTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGTCAATTTC‑AATATTGTCGctagt                                                                                                                                   <  2:353887/240‑5 (MQ=255)                                                                                                               gATTACGCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCACCTTGCGAGGGTTCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑ACCATTGTCGCCATTGCTc                                                                                                                               >  2:512921/1‑251 (MQ=255)                                                                                                                    cGCAATCATCTTCCGAATACAGTAGCAGTTTCTGCGTTCCGCAAAGCGACTGGGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTGACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGTGCCAATATC‑AACATTGTCGCCATTGCTCAGGGa                                                                                                                          <  2:576449/251‑1 (MQ=255)                                                                                                                     gCAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGGACGGCTGGCCATTATCTCGGTGGTAGGTGGTGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATACC‑AACATTGTCGCCATTGCTCAGGGAt                                                                                                                         >  2:607770/1‑251 (MQ=255)                                                                                                                      cAATCATCTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGGATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAggggtc                                                                                                                        >  2:109474/1‑248 (MQ=255)                                                                                                                         tcatcTTCCGAATACAGTATAAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTTCGGGAtcttc                                                                                                                     >  2:107886/1‑251 (MQ=255)                                                                                                                           atcatctgcaatcagtcacAGTTGCTGCGGTCCGCAAAGCGACTGGGGGCGAGCTGATCGGGCAATGCAGGAAGAGTGCGACCTGGAACTGAAAGAAGGCTTACTGGAGTCGC‑TGGCGGTGAC‑GGAACGTCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCCTGCGTGGGTTCTCGGCGAATTTCTTTGCCGCGCTGGCCCGCGCCACTAGC‑AACATTGTCGCCATTGCTCAGGGATCTTCTg                                                                                                                   <  2:371223/233‑1 (MQ=255)                                                                                                                            tcTTCCGAATACAGTATCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTg                                                                                                                   >  1:87486/1‑250 (MQ=255)                                                                                                                            tcTTCCGAATACAGTATCAGATTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGTAGGTTTGCGCACCTTGCGGGGGATCTCGGCGAAATTCTTTGCCGCAGTGGGCCTCGCCAATATC‑AACATTGTACCAATTTCACAGGGAtcttct                                                                                                                    >  2:454678/1‑249 (MQ=255)                                                                                                                               tCCGAATACAGTATCAGTTTCTTCGTTCCGCAAAGCGACTGTGTGCGATCTGAACGGGCAATGCAGGAAGAGTTCTTCCTGTAACTGAAAGAATGCTTACGGGAGGCGC‑TGGCGTTGAC‑GGAACGGCTGGCCATTATCGCGGGGGTAGGTGATGGTAGGCGCACCTGGCGTGGGATCTCGTCGAGATTCTTTGCCGCGCTTGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAAc                                                                                                                <  1:316492/250‑1 (MQ=255)                                                                                                                                           aTCAGTTTCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGGTGGTATACGCACCTTGCGGGGGATCTCGGCGAAATTCTTTTCCGCGCTGGCCCGCGCCAATATCCAAC‑TTGTCGCAATTGCTCAGGGATCTTCTGAACGCTCaacctattt                                                                                                   >  2:142546/1‑244 (MQ=255)                                                                                                                                           aTCAGTTGCTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGt                                                                                                   <  2:267049/251‑1 (MQ=255)                                                                                                                                                   cTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTGCCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCCTTGCTCAGGGATCTTCTGAACGCCCAATCTCTGT‑CGTGGTa                                                                                           >  2:431895/1‑250 (MQ=255)                                                                                                                                                   cTGCGTTCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGTTCTTCTGACCGCTCCATCTCTGT‑CGTGGTa                                                                                           >  1:485518/1‑250 (MQ=255)                                                                                                                                                      cgttacgcaAAGCGACTGTGTGCGAGCTGAACGGGCGATGCAGGAAGAGTTCTACCGGGAACGGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGAGCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATa                                                                                       <  2:478149/244‑1 (MQ=255)                                                                                                                                                        ttCCGCAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAAc                                                                                     >  1:488491/1‑251 (MQ=255)                                                                                                                                                         tccgcaAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGCACGCTCAATCTCTGT‑CGTGGTAAATAACg                                                                                    >  1:291082/5‑251 (MQ=255)                                                                                                                                                              aaaGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGGGGTAAATAACgatgat                                                                               >  1:253763/1‑251 (MQ=255)                                                                                                                                                                aGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTTT‑CGTGGTAAATAACGATGATGc                                                                             <  1:113115/251‑1 (MQ=255)                                                                                                                                                                aGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACGGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGc                                                                             <  2:231809/251‑1 (MQ=255)                                                                                                                                                                   gACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGCC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAACTTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGAc                                                                          >  1:296407/1‑251 (MQ=255)                                                                                                                                                                    aCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGTACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGCC‑GGCCCGGCTGGCCATTATCTCGGTGGTAGGTGATGGTTTGAGCACCTTGCGTGGGATTTCGGCGATATTCTTTGCCGCGCTGGCCCGCGCCAATTTC‑AACATTGTCGCCATTGCTTCGGGTTCTTCTGCACGCTCAATCTCTGT‑CGTGGTAAATAACGTTGATGCGAcc                                                                         >  2:326673/1‑251 (MQ=255)                                                                                                                                                                    aCTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCCTTTTCTCGGTGGTAGGGGATGGGATGAGGACAATGCGTGGGAACTCGGCGAAATTCATTGACGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTTTGCACGCTCACTCTTTGG‑CGTGGCAAATAACGATGATGCgccc                                                                         >  2:545654/1‑248 (MQ=255)                                                                                                                                                                             gAGCTGAACGGGCAATGCAGGAATAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCTTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCTCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTg                                                                <  1:576476/251‑1 (MQ=255)                                                                                                                                                                             gAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTTAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTg                                                                >  1:434953/1‑251 (MQ=255)                                                                                                                                                                              aGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTgc                                                               <  1:185186/251‑1 (MQ=255)                                                                                                                                                                                cTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTgcgc                                                             >  1:475509/1‑251 (MQ=255)                                                                                                                                                                                  gAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGt                                                           >  1:133861/1‑251 (MQ=255)                                                                                                                                                                                     cGGGCAATGCAGGAAGAGTTCTGCCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTAc                                                        <  1:431895/251‑1 (MQ=255)                                                                                                                                                                                      gggCAAGGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATGGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCGCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACt                                                       <  2:384469/251‑1 (MQ=255)                                                                                                                                                                                           aTGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCAGTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACtcatca                                                  <  1:616708/251‑1 (MQ=255)                                                                                                                                                                                             gCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAg                                                 <  1:294235/250‑1 (MQ=255)                                                                                                                                                                                             gCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGGGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAAAGATGATGCGAACACTGGCGTGCGCGTTACtcatc                                                   >  1:486228/1‑248 (MQ=255)                                                                                                                                                                                              cAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATATCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCCGAt                                               >  1:265079/1‑251 (MQ=255)                                                                                                                                                                                              cAGGAAGAGTTCTACCTGGAACGGTAAGAAGGCTTTCTGGAGCCGC‑TGGCGGTGAC‑GGAGCGGCTGGCCATTATCACGGGGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCGGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGAGCGTCTGAACGCTCAATCGCTGT‑CGTGGTAAATATCGATGATGCGACCACGGGCGAGCGCGTTACTCATCAGAt                                               <  2:273300/251‑1 (MQ=255)                                                                                                                                                                                               aGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATg                                              >  1:125366/1‑251 (MQ=255)                                                                                                                                                                                                     aggtCTACCTGGAACTGAAAGAAGGGTGACGGGAGCCGCGTGG‑GGTGAC‑GGGACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCGCCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCGCAGGGATCGTCGGAACGCTCAATCTCTGT‑CGTGGTAAATAACTATGATTCGACCACTGGCGTGCGCGTGACTGATCAGATGCTGTTc                                        <  2:65395/248‑1 (MQ=255)                                                                                                                                                                                                           gccTGGAATTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATAcc                                  <  2:396849/250‑1 (MQ=255)                                                                                                                                                                                                           aacTGGAACTGAAAGAAGGCTTACGGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTGCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGGGCGCGTTACTCATCAGATGCTGTTCAAt                                     <  2:291082/246‑1 (MQ=255)                                                                                                                                                                                                           aCCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATAc                                   <  1:529252/250‑1 (MQ=255)                                                                                                                                                                                                           aCCTGGAACGGAAAGAAGGCTTTCTGGAGCCTC‑TGGCGGTTAC‑GGAACGGCTGGCCATTATCTCGTTGGTAGGTGATGGTATTCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTTGCCCGCGCTAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAAt                                     <  1:480550/248‑1 (MQ=255)                                                                                                                                                                                                                gAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGGAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTTTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATATCTGT‑CGTGGTAAATAACGATGATGGGCCAACTGGCGTGCGCGGTAATACTCAGATGCGTTTCAATCCCGATc                              >  2:509294/1‑250 (MQ=255)                                                                                                                                                                                                                 aaCTGAAAGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATc                              >  1:335477/1‑249 (MQ=255)                                                                                                                                                                                                                     gAAAGAAGGCGTACTTGAGCCGC‑TGGAGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATAGCGATCAGGtt                         <  2:259491/250‑1 (MQ=255)                                                                                                                                                                                                                      aaaGAAGGCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTAt                       <  1:224192/251‑1 (MQ=255)                                                                                                                                                                                                                            ggCTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAgg                 >  1:512940/1‑250 (MQ=255)                                                                                                                                                                                                                              cTTACTGGAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAgtgt               >  1:53714/1‑251 (MQ=255)                                                                                                                                                                                                                                tgcTGGAGCCGC‑TGGCGGTGGC‑GGGACGGTTGGCCACTATCTCGGGGGTAGGTGATGGTATGCGCGCCGTGCGTGGGATCTCGGCGCAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGTTCTTCTGAACGCTCAATCGCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGCTTATCGAAgtgt               <  2:75706/247‑1 (MQ=255)                                                                                                                                                                                                                                    gtattcGC‑TGGCGGTGAC‑GGTACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCGGAATGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCTTTACTCATCAGATGCTGTTCAATACCGCTCAGGTTATCGAAGTGTTtgt           <  1:44995/244‑1 (MQ=255)                                                                                                                                                                                                                                    ggAGCCGC‑TGGCGGTGAC‑GGAACGGGGGGCCATTATCTCGGTGGTAGGGGATGGTATGCGCTCCGTGCGTGGGATCGCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTC‑GTGCGTGGTAAATAACGAGGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGGTCAATACCGATCAGGTTCGCGAATTGTATGTGa         <  2:562241/251‑1 (MQ=255)                                                                                                                                                                                                                                     gAGCCGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTCTGTGAt        <  1:215259/251‑1 (MQ=255)                                                                                                                                                                                                                                        ccGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGGTATCGAAGTGTTTGTGATTgg     >  1:527310/1‑251 (MQ=255)                                                                                                                                                                                                                                         cGC‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGGAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGc    >  2:448544/1‑251 (MQ=255)                                                                                                                                                                                                                                           c‑TGGCGGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTCGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCGCTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGt  >  2:79192/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                         CTGAATAACATGGCAATGTTCAGCGTTTCTGGTCCGGGGATGAAAGGGATGGTCGGCATGGCGGCGCGCGTCTTTGCAGCGATGTCACGCGCCCGTATTTCCGTGGTGCTGATTACGCAATCATCTTCCGAATACAGCATCAGTTTCTGCGTTCCACAAAGCGACTGTGTGCGAGCTGAACGGGCAATGCAGGAAGAGTTCTACCTGGAACTGAAAGAAGGCTTACTGGAGCCGC‑TGGCAGTGAC‑GGAACGGCTGGCCATTATCTCGGTGGTAGGTGATGGTATGCGCACCTTGCGTGGGATCTCGGCGAAATTCTTTGCCGCACTGGCCCGCGCCAATATC‑AACATTGTCGCCATTGCTCAGGGATCTTCTGAACGCTCAATCTCTGT‑CGTGGTAAATAACGATGATGCGACCACTGGCGTGCGCGTTACTCATCAGATGCTGTTCAATACCGATCAGGTTATCGAAGTGTTTGTGATTGGCGT  >  NC_000913/1270‑1754

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑