Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	14,223	G→A	R19H (CGT→CAT)	dnaJ →	chaperone protein DnaJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	14,223	0	G	A	98.2%	166.7 / NA	54	R19H (CGT→CAT)	dnaJ	chaperone protein DnaJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/22);  minor base C (1/0);  total (32/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGAT  >  NC_000913/13980‑14467                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     tGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCATCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATc                                                                                                                                                                                                                                               >  2:164523/1‑251 (MQ=255)   cccAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAAGAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGTGCAATTTAAAATAGATGGCTAAGCAAGATTATGACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCaa                                                                                                                                                                                                                                             <  1:230074/251‑2 (MQ=255)       gcagcagACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAg                                                                                                                                                                                                                                         <  2:155074/251‑1 (MQ=255)            agACTGCAGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTCCCTCTCCGCCCGTGCTTTCAGCTAGGGGCAATTTAAAAAAGATGGCGAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTa                                                                                                                                                                                                                                    <  1:386705/251‑1 (MQ=255)                 gCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCAGCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAAc                                                                                                                                                                                                                               <  1:263743/251‑1 (MQ=255)                       gCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTTACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTgg                                                                                                                                                                                                                         <  1:411403/251‑1 (MQ=255)                                      aacaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCCAAAAGGCCTACAAACGCCTGGCCATGAAATaccacc                                                                                                                                                                                                          >  1:350723/1‑251 (MQ=255)                                       acaacGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCAccc                                                                                                                                                                                                         >  1:457560/1‑251 (MQ=255)                                                        gatggtgtcgacgtataaTTTGAAGAAGGCAAAGACAAAAAATAATCGCCCTAGAAACGGGTAATTATACTGACACGGGCGAAGGGGAATATCCCCTCCGCCCGTGCATTCATCTAGGGGCAATTGAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGATTCCAAAACAGCTCAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACGACCCGGACCGTTACCAGGGTg                                                                                                                                                                                        <  2:422266/235‑1 (MQ=255)                                                             tgtCGACGCTGAAGTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACTGGTAATTATACTGACGCGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTGAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTGGGGGTTGCCAAAACAGCGGAAGAGCAGGAAATCAAAAATGCCTACAAACGCCTTGCCATGAAAGACCCCCCGGACCGTAACCAGGGTGACaa                                                                                                                                                                                    <  2:285105/250‑1 (MQ=255)                                                                cGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAaga                                                                                                                                                                                 <  1:329984/250‑1 (MQ=255)                                                                 gACGCTGAATTTGGAGAAGTCAAAGACAAAAAATAATCGCCCTATACACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACACAGAgg                                                                                                                                                                               >  2:114192/1‑251 (MQ=255)                                                                        aaTTTGAAGAAGTCAAAGACAAAAAATAATCGCCCAATAAACGGGTAAGTATACGGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAACCAAAAAGGCCTACAAACGCCTGGCCAGGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAggt                                                                                                                                                                        <  2:102921/251‑2 (MQ=255)                                                                              aagaagTCAAAGACAAAAAATAATCGACCTCTAAACGGGTAATTATACTGACACGGGCGAAGGGGACATTACTCTCCGCCCGTGCATTCATCTAGGAGCAATATAAAAAAGATGACTAAGCCAGATAATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGACATGCAATACCACCCGGACCGTAACACGGGTGACAACGAGGCCGAGTCGAAAtt                                                                                                                                                                  >  1:277192/1‑251 (MQ=255)                                                                                gaagTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAAttt                                                                                                                                                                 >  2:122123/1‑250 (MQ=255)                                                                                  agTCAAAGACAAAAAATAATCGCCCTATAAACGGGGAATTATACTGACACGGGCGAAGGGGCATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCACAACAGCGGAAGAGCCTGAAATCCAAAAGGCCTACAAACGACTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAACtttca                                                                                                                                                               >  2:250228/1‑248 (MQ=255)                                                                                   gTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAag                                                                                                                                                             <  1:618521/251‑1 (MQ=255)                                                                                        aGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGCAATTTAAag                                                                                                                                                             >  1:207149/1‑246 (MQ=255)                                                                                        aGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATc                                                                                                                                                        <  2:260771/251‑1 (MQ=255)                                                                                           cAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTAATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCa                                                                                                                                                       >  1:255550/1‑249 (MQ=255)                                                                                                   aatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTa                                                                                                                                             <  2:287484/251‑1 (MQ=255)                                                                                                   aatCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTACTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAGCCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGATATCAAGGAAGCtt                                                                                                                                              <  1:189856/250‑1 (MQ=255)                                                                                                      cGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATg                                                                                                                                           >  1:342158/1‑250 (MQ=255)                                                                                                                 cGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCg                                                                                                                               >  1:7374/1‑251 (MQ=255)                                                                                                                  gggTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCg                                                                                                                               >  1:183396/1‑250 (MQ=255)                                                                                                                             cTGACCCGGGCGCAGGGGAATTTCCTCTCAGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTCCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAACTTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAAc                                                                                                                   >  2:372929/1‑251 (MQ=255)                                                                                                                                acacGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTg                                                                                                                >  1:121575/1‑251 (MQ=255)                                                                                                                                   ggggCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCgg                                                                                                             <  2:1652/250‑1 (MQ=255)                                                                                                                                   cGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGCTTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCgg                                                                                                             >  1:595078/1‑251 (MQ=255)                                                                                                                                             ggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGc                                                                                                                                                                        >  2:610488/1‑182 (MQ=255)                                                                                                                                             ggAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGc                                                                                                                                                                        <  1:610488/182‑1 (MQ=255)                                                                                                                                                    ccTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGt                                                                                            <  2:173708/251‑1 (MQ=255)                                                                                                                                                         ccGCCAGTGCATTCATCGAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATGTTAGGCGTTGCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCACGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTGAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGTCATACGATCAGTATGGTCAtg                                                                                        <  2:452227/250‑1 (MQ=255)                                                                                                                                                            cccGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCAtgctgc                                                                                    <  2:490811/251‑1 (MQ=255)                                                                                                                                                                 gCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGCGATTTTAGGCGTTTCCAAACCAGCGGAAGAGCATGCAATCAACACGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTACCCAGGGTGCCAAAGAGGCCGAGGCGAACTTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTg                                                                               >  1:408398/1‑251 (MQ=255)                                                                                                                                                                 gCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAATAGGCCGAGGCGAAATTTAAAGAGATCAAGTAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGttt                                                                                <  1:355752/250‑1 (MQ=255)                                                                                                                                                                 gCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGttt                                                                                <  2:19085/250‑1 (MQ=255)                                                                                                                                                                                      aaaaaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGggcggcg                                                          >  2:298021/1‑251 (MQ=255)                                                                                                                                                                                       aaaaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGggcggcgg                                                         >  1:291452/1‑251 (MQ=255)                                                                                                                                                                                         aaaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGCCAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGCTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGgcggcggcg                                                       >  1:85903/1‑251 (MQ=255)                                                                                                                                                                                          aaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGcggcggcgg                                                      <  1:584826/251‑1 (MQ=255)                                                                                                                                                                                          aaGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGcggcggcgg                                                      >  1:212398/1‑251 (MQ=255)                                                                                                                                                                                                   aaGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTggcgg                                             >  2:574392/1‑251 (MQ=255)                                                                                                                                                                                                       aaGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTggcggcggc                                         >  1:407944/1‑251 (MQ=255)                                                                                                                                                                                                             attaCGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGAACCGTAACCAGGGTGACAAAGCGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGAGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGAGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCCGAc                                   >  1:23069/1‑251 (MQ=255)                                                                                                                                                                                                                  gagaTTTTAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTc                                >  1:292777/1‑249 (MQ=255)                                                                                                                                                                                                                        ttaggctattCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGata                         <  2:172070/242‑1 (MQ=255)                                                                                                                                                                                                                         tAGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGACATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATAtt                       >  2:354066/1‑251 (MQ=255)                                                                                                                                                                                                                          aGGCGTTTCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATAttt                      >  1:163760/1‑251 (MQ=255)                                                                                                                                                                                                                                ttCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTg                >  1:153858/1‑251 (MQ=255)                                                                                                                                                                                                                                 tCCAAAACAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTg                >  1:212284/1‑250 (MQ=255)                                                                                                                                                                                                                                     aaaCAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGtt           >  1:263300/1‑251 (MQ=255)                                                                                                                                                                                                                                       aCAGCGGAAGAGCATGAAATCAAAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGtttt         >  2:439084/1‑251 (MQ=255)                                                                                                                                                                                                                                               agagCATGACATCAAAAAGGCCTACAACCGCCTGGCCCTGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGCAATTTAAAGAGATAACGGACGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGTATGGGCGGCGGCGGTTTTGGCGGCGGCGCCGACTTCAGCGATATTTTTGGTGACGGTTTCGGCGat  >  2:626390/1‑250 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                     TGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATACTGACACGGGCGAAGGGGAATTTCCTCTCCGCCCGTGCATTCATCTAGGGGCAATTTAAAAAAGATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAAATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGACAAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCGCAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGCGGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGAT  >  NC_000913/13980‑14467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑