Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,866	G→A	A568A (GCG→GCA)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,866	0	G	A	100.0%	193.1 / NA	60	A568A (GCG→GCA)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (30/30);  total (30/30)

CGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATA  >  NC_000913/13623‑14103                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              cgcgAAAGATAAAAACAGCGTTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGAAGCATAAGCTAACGCTGAAGCTGACCGTCAGTTTGAATAGCTGGTACAGACTCGCAACCAGGGCGTCCATCTGCTGCACA‑GCACCAGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCactgactg                                                                                                                                                                                                                                        <  1:67996/251‑1 (MQ=255) cgcgAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAAATGAAATCCAGAAAATGTGACACGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGCCCAGCTGCCGCACA‑TCCCCCGTAAGCATGTTGAAGAAGCTTGCGACAAACTGCCGGCGGACGACAAAACTGCTAGCGAGTCTGCACTGACtt                                                                                                                                                                                                                                        <  1:68001/251‑2 (MQ=255)    gAAAGATACAATCAGCGGTAAAGAGCCGAAGCTCACCATAAAGGCTTCTTCTGGTCTGAACGAAGATGCAATCCAGAAAACGGAACGCGACGCAGAAGCTCAAGCCAAAGCTGACCGTACGTTTGACGAGCTCGTACAGACTCGCAACCAGAGCGACCACCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGCCAACCTGCCGGATAAAGACAACACTGCTATCGAGTCTGCACTGACTGCAc                                                                                                                                                                                                                                     >  2:230074/1‑251 (MQ=255)               aCAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCGGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGct                                                                                                                                                                                                                          <  1:429172/251‑1 (MQ=255)                cAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGctc                                                                                                                                                                                                                         >  1:260771/1‑251 (MQ=255)                  gcggtgaggagCAGAAGATCACCTTCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCAAGCAAATGGTATGCGACGCAGCAGCTAACGCAGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACAAGGGCGACCATCTGCTGCACA‑GCACCCGTGAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGCCGACATAACTGATATCGAGTCTGCACTGACTGCACGGGAAACTGCTCTg                                                                                                                                                                                                                       >  2:263743/9‑251 (MQ=255)                  gCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTg                                                                                                                                                                                                                       <  2:135453/251‑1 (MQ=255)                   cgatAAAGAGCAGCAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAAAGCCGAAGCTGACCGAAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGCACATCTGCTG‑ACAAGCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATAGAGTCTGCAATGACTGCAATGGAAACTGctata                                                                                                                                                                                                                       >  2:329984/4‑247 (MQ=255)                     gTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGCGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGctct                                                                                                                                                                                                                                    <  2:492056/235‑4 (MQ=255)                     gTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACt                                                                                                                                                                                                                                    >  1:492056/1‑235 (MQ=255)                         agagCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTg                                                                                                                                                                                                                >  1:172070/1‑251 (MQ=255)                          gagCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGa                                                                                                                                                                                                               <  2:162699/251‑1 (MQ=255)                                     cACCATCCAGGCTTCTTATGGACTGAAAGACGATGAAATCCAGCAAATGGTACGCGACGCAGAAGCTAACCCCGAAGCTGACCGTAAGTTTGAAGAGCTGGGACAGACTCGCAACCAGGGCGAACATCTGCTGCACA‑GAACCCGTAAGCCGGTTGCAGAAGCAGGCGACAAACTGACGGCTGACGACCAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAACGGTGAAGACAAAGCCg                                                                                                                                                                                                    >  2:411403/1‑251 (MQ=255)                                       ccATCAAGGCTTCTTCTGGTCTGAACGAATATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCt                                                                                                                                                                                                  <  1:136658/251‑1 (MQ=255)                                         aTCAAGGCTGCTTCTTGTATGAACGAAGATTAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAATAGCTGGTGCAGACTCGCAACCAGGGCGACCATCTGCTGCTCA‑GTACCCGTAAGCAGGTTGAAGAAGCAGTCGAGAAATTGCGGGCTGACGACATAACTGCTATCGAGTCGGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGATGTCAAAGCCGCTAt                                                                                                                                                                                                <  2:66598/251‑1 (MQ=255)                                           cAAGGCTTCTTCGGGGCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGTAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGCCAAAGCCGCTATCg                                                                                                                                                                                              <  1:185239/251‑1 (MQ=255)                                                  tcttctGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGCCCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGCCAAAGCCGCTATCGACGCGa                                                                                                                                                                                        >  1:102921/1‑250 (MQ=255)                                                    ttctGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGACGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGaaa                                                                                                                                                                                      >  1:397182/1‑250 (MQ=255)                                                     tctGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAAt                                                                                                                                                                                    >  1:155074/1‑251 (MQ=255)                                                              aaCGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGagct                                                                                                                                                                           >  1:169583/1‑248 (MQ=255)                                                                              aGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAg                                                                                                                                                           <  1:529099/251‑1 (MQ=255)                                                                               gAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGa                                                                                                                                                          <  1:265310/251‑1 (MQ=255)                                                                                     ggTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTg                                                                                                                                                     >  1:215933/1‑250 (MQ=255)                                                                                         cgcgACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGATGGCACAGGTTTCCCAGAAACTGATgg                                                                                                                                                 >  2:502198/1‑250 (MQ=255)                                                                                            gACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAAt                                                                                                                                             >  1:173708/1‑251 (MQ=255)                                                                                              cGCAGAAGCTAACGCCGAAGCTGACCGTCAGTTTGCAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTCAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACt                                                                                                                                                                                                                                         >  2:395817/1‑157 (MQ=255)                                                                                              cGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACt                                                                                                                                                                                                                                         <  1:395817/157‑1 (MQ=255)                                                                                                  gAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCca                                                                                                                                       <  1:298508/251‑1 (MQ=255)                                                                                                   aaGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCcag                                                                                                                                      <  1:491836/251‑1 (MQ=255)                                                                                                    aGCTACCGCCGACGCTGACCGTACGTTTGAAGCGCTGGTCCAGCCGCGCTACCAGGGCGACCATCTGCTGCACA‑GCACCCCTAAGCAGGATGCAGAAGCCGGCGACAACCTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGCACGGTGAAGACAACGCCGCTATCGAAGCGCAAATGCAAGTGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCcagc                                                                                                                                     >  2:316650/1‑251 (MQ=255)                                                                                                    aGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGAAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCcagc                                                                                                                                     >  2:512798/1‑251 (MQ=255)                                                                                                        aaCGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAgcagca                                                                                                                                 <  2:437083/251‑1 (MQ=255)                                                                                                         aCGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCaa                                                                                                                                <  1:173734/251‑1 (MQ=255)                                                                                                               aaGCTGACCGTACGTTTGAAGAACTGGTACCGACTCGCAACCACCACGACAATCCGCTGCACA‑GAACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACAGCCGCCTGACGACAAAACTGCTATCGAGTCTGCACTGCCTGCACTGGAAACTGCTCTGAAAGGTGAAGACAACGCCGCTATCGAAGCGAAAATGCAGGAGCAGGCACAGGCTTCCCAGATACTGATGGAAATCGCCCAGCAGCAACATgac                                                                                                                          >  2:584826/1‑249 (MQ=255)                                                                                                                 gCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCCCA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCca                                                                                                                        <  2:437291/251‑1 (MQ=255)                                                                                                                          aaGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGAc                                                                                                               >  2:455342/1‑251 (MQ=255)                                                                                                                                           aCAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCGCCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCtt                                                                                              <  1:86704/251‑1 (MQ=255)                                                                                                                                           aCAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGATAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCt                                                                                               <  1:222997/250‑1 (MQ=255)                                                                                                                                               aCTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGc                                                                                          >  1:494562/1‑251 (MQ=255)                                                                                                                                                  cGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCa                                                                                         <  1:58549/249‑1 (MQ=255)                                                                                                                                                   gCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAaac                                                                                      <  2:150394/251‑1 (MQ=255)                                                                                                                                                   gCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAaac                                                                                      >  1:452227/1‑251 (MQ=255)                                                                                                                                                             cGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGtt                                                                                                                                                                 <  1:231155/166‑1 (MQ=255)                                                                                                                                                             cGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGtt                                                                                                                                                                 >  2:231155/1‑166 (MQ=255)                                                                                                                                                               aCCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCGGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCGATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGAt                                                                          <  1:597722/251‑1 (MQ=255)                                                                                                                                                                 cATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATgg                                                                        >  2:66123/1‑250 (MQ=255)                                                                                                                                                                               gCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGAc                                                           >  1:1652/1‑251 (MQ=255)                                                                                                                                                                                  cccGTAAGCAGGGTGAAGAAGCAGGCGACAAACTGCCGGCGGACGACAAAACTGCTATCGAGTCTGCACTGACTGCAATGGAAACCGCTCTGAAAGGGGAAGACAAAGCCGCTATCGGAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGTAACTGTTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCGGACTGTCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGCCGATGTTGTCGACGCt                                                        <  2:463894/251‑1 (MQ=255)                                                                                                                                                                                                  agaagCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACg                                                                      <  2:88146/221‑1 (MQ=255)                                                                                                                                                                                                  agaagCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACg                                                                      >  1:88146/1‑221 (MQ=255)                                                                                                                                                                                                      gCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGa                                    <  1:422257/251‑1 (MQ=255)                                                                                                                                                                                                           cGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACaaaa                               <  2:169583/251‑1 (MQ=255)                                                                                                                                                                                                            gACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGAACTGGCAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAACGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAACATGCTGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGAAAAAAACGCGAAAGCTGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACaaaa                               >  1:285105/1‑250 (MQ=255)                                                                                                                                                                                                                    gCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGACACCGCTCTGCAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGCGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGGTGACGCTGTTGTCGACGCTGAATTTGAAGAAGTCAGAGACAAAACATAATCGc                      >  2:189856/1‑251 (MQ=255)                                                                                                                                                                                                                    gCCGGCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATTGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGGCAAAGACAAAAAATAATCgg                      >  2:197023/1‑250 (MQ=255)                                                                                                                                                                                                                       ggCTGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCTGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGAATGCCGGTGCTAATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCTACGCTGAATATGAAGAAGTCAAAGACAAAAAATAATCGCCCt                   <  2:596906/251‑1 (MQ=255)                                                                                                                                                                                                                          tGACGACAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCtat                 >  1:452080/1‑250 (MQ=255)                                                                                                                                                                                                                             cgacAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAAc             >  1:490811/1‑251 (MQ=255)                                                                                                                                                                                                                                cAAAACTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACggg          <  1:131427/251‑1 (MQ=255)                                                                                                                                                                                                                                  aaaCTGCTATCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGACAAAGACAAAAAATAATCGCCCAATAAACGGGTa        <  2:494562/251‑1 (MQ=255)                                                                                                                                                                                                                                          aTCGAGTCTGCACTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAGCTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATtata  <  1:344219/249‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                              CGCGAAAGATAAAAACAGCGGTAAAGAGCAGAAGATCACCATCAAGGCTTCTTCTGGTCTGAACGAAGATGAAATCCAGAAAATGGTACGCGACGCAGAAGCTAACGCCGAAGCTGACCGTAAGTTTGAAGAGCTGGTACAGACTCGCAACCAGGGCGACCATCTGCTGCACA‑GCACCCGTAAGCAGGTTGAAGAAGCAGGCGACAAACTGCCGGCTGACGACAAAACTGCTATCGAGTCTGCGCTGACTGCACTGGAAACTGCTCTGAAAGGTGAAGACAAAGCCGCTATCGAAGCGAAAATGCAGGAACTGGCACAGGTTTCCCAGAAACTGATGGAAATCGCCCAGCAGCAACATGCCCAGCAGCAGACTGCCGGTGCTGATGCTTCTGCAAACAACGCGAAAGATGACGATGTTGTCGACGCTGAATTTGAAGAAGTCAAAGACAAAAAATAATCGCCCTATAAACGGGTAATTATA  >  NC_000913/13623‑14103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑