Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	13,014	A→G	P284P (CCA→CCG)	dnaK →	chaperone protein DnaK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	13,014	0	A	G	100.0%	217.4 / NA	68	P284P (CCA→CCG)	dnaK	chaperone protein DnaK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (42/26);  total (42/26)

TTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTA  >  NC_000913/12773‑13249                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            ttatCGACATCCGCGAAGTTGACGACGCAAATACCTTCGAAGTTCTGAAAACCAAAGATGATACCCAGATGGGAGGTAACG‑CCTTCGACAGCCGTCTGCTCAACTCCATGGTTGAAGAATTCAAGAAAGCTCAGGGCATTGACCTGCGCAACGATCCGCTGGCACTGCAGCGCCTGAAAGTAGCGGCAGCAAAAACGAAAATCGACCTGTCTTCCGCTCAGCAGCCCGACGTTCACCTTCCGAACATCACt                                                                                                                                                                                                                                    >  2:309762/1‑251 (MQ=255) ttatCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACt                                                                                                                                                                                                                                    >  1:484449/1‑251 (MQ=255)     cGAAATCGCCGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGACGTACATCACTGc                                                                                                                                                                                                                                  >  2:394471/1‑249 (MQ=255)        aaTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGatg                                                                                                                                                                                                                             >  2:286925/1‑249 (MQ=255)         aTCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCTCCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGTGTATTTACCTGCGCAACGATCCGCTGTCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGatgc                                                                                                                                                                                                                            <  1:591604/251‑4 (MQ=255)            gacgaAGTTGACGGCGAAAAAACCTTCGACGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGAc                                                                                                                                                                                                                         >  2:185390/1‑251 (MQ=255)              cgaATTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTTCCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTACCCTGCCGTACATCGCTGCAGATGCGACCg                                                                                                                                                                                                                       <  1:482719/251‑1 (MQ=255)                   ttGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGATGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGACTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCg                                                                                                                                                                                                                  >  1:375631/1‑251 (MQ=255)                        ggCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGACGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCGCTGCAGATGCGACCGGTCCGAAaca                                                                                                                                                                                                             <  2:349468/251‑1 (MQ=255)                         gCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTACCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAacac                                                                                                                                                                                                            <  2:162937/251‑1 (MQ=255)                                      cGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAACGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGCTGCGACCGGTCCGAAACACATGAACATCaa                                                                                                                                                                                                 >  1:359086/1‑249 (MQ=255)                                          gTTCTGTCAACCAACGGTGAGACCCACTTGGGTGGTGAAG‑ACTGCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATGGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACGGTCTTCCGCGCAGCAGACCGACGTTAACCTGCCGTACATCACTGGAGATGCGACCGGTCCGAAACACAGGAACATCAAAGttac                                                                                                                                                                                           <  2:62050/251‑4 (MQ=255)                                          gTTCTGGCAACCAACGGTGATACCCCCCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGGTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGAc                                                                                                                                                                                           <  2:53247/251‑1 (MQ=255)                                               ggCAACCAACGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCg                                                                                                                                                                                        <  1:6800/249‑1 (MQ=255)                                                      aacGGTGATACCCACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACt                                                                                                                                                                               >  1:79777/1‑251 (MQ=255)                                                            gatccccACCTGGGTGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCGGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACAGGAACATCAAAGTGACTCGTGCGAAACTGGAAAg                                                                                                                                                                         <  2:359086/247‑1 (MQ=255)                                                            aaaaCCCACCTGGGTGGTGAAG‑ACTTCGAATGCCGTCTTAGCAACTTCCTGGGTGAAAAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAATTGACTCGTGCGAAACTGGAAAg                                                                                                                                                                         <  2:80329/248‑1 (MQ=255)                                                                cccACCTGGGGGGAGAAGCACT‑CGCCACCAGTCTGATCACCTACCTGGTTGAAGAATTCAGGAACGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGACAAAGCGAAAATCGAACTGTCTTCCGCTAAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACCTGAACATCAAAGTGCCTCGTGCGAAACTGGGAAGCCTg                                                                                                                                                                     >  2:621188/1‑251 (MQ=255)                                                                     cTGGGTGGTGAAG‑ACTTCGCCAGCCGTCTGATCACCTACCTGGTTGAAGAATTCAAGAAAGATAAGGGTCTTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTg                                                                                                                                                                 >  2:62082/1‑250 (MQ=255)                                                                        ggtggTGAAG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGa                                                                                                                                                             <  1:41494/248‑1 (MQ=255)                                                                              gAAG‑ACTTCGACAGCCGTCTGATTAACTACCTGGTTGAAGAATTCAATAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTTCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTgg                                                                                                                                                        <  1:393122/250‑1 (MQ=255)                                                                                aG‑ACTTCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTaa                                                                                                                                                     <  1:394471/251‑1 (MQ=255)                                                                                      tCGACAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAcc                                                                                                                                                  <  1:352166/249‑1 (MQ=255)                                                                                         aCAGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGCAGATCTGGTAAACCGTTcc                                                                                                                                             >  1:605826/1‑251 (MQ=255)                                                                                           aGCCGTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCAt                                                                                                                                           >  2:5916/1‑251 (MQ=255)                                                                                               gTCTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTg                                                                                                                                         >  1:65167/1‑249 (MQ=255)                                                                                                 cTGATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTG‑CAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGcc                                                                                                                                     >  2:418949/1‑250 (MQ=255)                                                                                                   gATCAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTG‑CAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGcc                                                                                                                                     <  1:418949/248‑1 (MQ=255)                                                                                                      cAACTACCTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTg                                                                                                                                 >  1:171869/1‑250 (MQ=255)                                                                                                             cTGGTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGAAACGATCCGCTGGAAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGCCCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGc                                                                                                                         >  2:412961/1‑251 (MQ=255)                                                                                                               tgTTGAAGAATTCAAGAAAGATCAGGGTATTGACCTGCGCAACGAGCCGCTGGCAATGCAGGGCCTGAAAGAAGCGTCCGAAAAATCGAAAATCGAACTGTGTTCCGCTCAGCAGTCCGATGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACAGCAAAGTGACGCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCa                                                                                                                        <  1:581646/249‑1 (MQ=255)                                                                                                                      gaaTTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAgg                                                                                                                 >  1:35673/1‑250 (MQ=255)                                                                                                                       aatgcAAGAAAGATCAGGGTATTGCCCTGCGCAACGATCCGCTGGCAATGCAGCTCCTGAAAGAAGCGGCAGAAAAAGCGAAACTCTAACTGTCTTCCGCTCATCAGACCGACGTTAACCTGCCGTACATCACTGCAGAGGCGACCGGTCCGAAACACATGAACAGCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGTGCCGCTGAAAGTTTCCCTGCAggcc                                                                                                               <  2:222136/247‑3 (MQ=255)                                                                                                                         ttCAAGAAAGATCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGc                                                                                                             >  1:366800/1‑251 (MQ=255)                                                                                                                             agaaagaTCCGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGc                                                                                                         >  1:517080/1‑251 (MQ=255)                                                                                                                               aaagaTCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCt                                                                                                       <  1:116228/251‑1 (MQ=255)                                                                                                                                aagaTCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGGAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTg                                                                                                      >  2:216500/1‑251 (MQ=255)                                                                                                                                  gaTCAGGGTATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGACTGTc                                                                                                    >  2:182391/1‑251 (MQ=255)                                                                                                                                              gACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGatat                                                                                        >  1:431767/1‑251 (MQ=255)                                                                                                                                                ccTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATcg                                                                                      >  2:379071/1‑251 (MQ=255)                                                                                                                                                         cGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGtt                                                                              >  1:594652/1‑250 (MQ=255)                                                                                                                                                          gATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTACGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGAATCTGATATCGACGACGTTAt                                                                            >  1:578417/1‑251 (MQ=255)                                                                                                                                                          gATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGAAGAAAAAGCGAAAAGCGAACTGTCTTCCGCTCAGCAGGCCGACGTTAACCTGCCGTACATCACGGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTTAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTAt                                                                            <  2:300814/251‑1 (MQ=255)                                                                                                                                                              cgctggacaTGCAGCGCATGAAAGAAGCGGCAGACAAAGCGAATATCGAACTGTATTCCGCTAAGCAGACAGAAGTTAACCTGCCGTACATCACTGCAGATGAGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGACAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGCTATCGAAGACGTTGTCCTc                                                                        >  2:408606/9‑251 (MQ=255)                                                                                                                                                                  ggCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTCCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGt                                                                      <  1:443930/249‑1 (MQ=255)                                                                                                                                                                   gCAATGCCGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACGGGAAAGCCGGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTtgg                                                                   <  2:605826/251‑1 (MQ=255)                                                                                                                                                                        gCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTc                                                              <  1:185390/251‑1 (MQ=255)                                                                                                                                                                        gCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTAGCCTCGTTggtggt                                                               <  1:137802/250‑1 (MQ=255)                                                                                                                                                                              ccTGAAAGAAGCTGCAGAAAAAGCGAAAATCGAAGTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTc                                                        <  2:265496/251‑1 (MQ=255)                                                                                                                                                                                           gCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGt                                           >  2:15055/1‑251 (MQ=255)                                                                                                                                                                                            cAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTACCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGGAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTAGCTGATATCGACGAAGTTATCCTCGTTGGTGGTCAGACTCGTAGGCCAATGGtt                                          >  2:145847/1‑251 (MQ=255)                                                                                                                                                                                             tgAAAAAGCGAAAGTCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCAATGCAGATGCGCCCGGTCCGAAACACATGAACATCATAGTGACTCGTGCGAACCTGGAACGCCTGATTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTGGAACTGCAGGACGTTGGCCTGTCCGTATCTGATTTAGACAACGTTATACTCGTTGGTGGTCaagcacggctgccaaatgtac                                         >  2:589289/2‑231 (MQ=255)                                                                                                                                                                                                 aaaGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCaga                                      >  1:558666/1‑250 (MQ=255)                                                                                                                                                                                                    gCGAAAATCGAACTGTCTGCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAgaagaa                                  <  2:366800/251‑1 (MQ=255)                                                                                                                                                                                                        acaTCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACAGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGCAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCATGTCCGTATCTGATATCGACGACGTTATCCTAGTTGGGGGTCCGGCTCGTATGCCAAAGGTTCAGAAGaaattt                              >  2:203574/3‑248 (MQ=255)                                                                                                                                                                                                           tCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGACAGTTgat                           >  1:554252/1‑249 (MQ=255)                                                                                                                                                                                                            cGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCt                           <  1:286925/250‑1 (MQ=255)                                                                                                                                                                                                             gAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTg                          >  1:162301/1‑250 (MQ=255)                                                                                                                                                                                                              aaCTGTCTTCCGCTCAGCAGACAGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGCAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGACAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTg                          >  2:559805/1‑249 (MQ=255)                                                                                                                                                                                                                     ttCCGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTg                 >  1:420784/1‑251 (MQ=255)                                                                                                                                                                                                                        cGCTCAGCAGACCGCCGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGt               >  1:469150/1‑250 (MQ=255)                                                                                                                                                                                                                        cGCTCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGt               >  1:431298/1‑250 (MQ=255)                                                                                                                                                                                                                           tCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAAACGTTCCATTg                                                                                                                                         >  1:608348/1‑125 (MQ=255)                                                                                                                                                                                                                           tCAGCAGACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACAGGAACATCAAAGTTACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCGGGTAAACCGTTCCATTg                                                                                                                                         <  2:608348/125‑1 (MQ=255)                                                                                                                                                                                                                             agcagACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGGTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAag           >  2:396952/1‑249 (MQ=255)                                                                                                                                                                                                                                 gACCGACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAACGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCcgc     >  2:220883/1‑251 (MQ=255)                                                                                                                                                                                                                                   ccGACGTTAACATGCAGTACATCACTGCAGATGCGACCGGTCCGCAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCcgcg    >  2:514502/1‑250 (MQ=255)                                                                                                                                                                                                                                     gACGTTAACCTGCCGTACATCACTGCAGATGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTa  >  2:581724/1‑250 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                            TTATCGAAATCGACGAAGTTGACGGCGAAAAAACCTTCGAAGTTCTGGCAACCAACGGTGATACCCACCTGGGGGGTGAAG‑ACTTCGACAGCCGTCTGATCAACTATCTGGTTGAAGAATTCAAGAAAGATCAGGGCATTGACCTGCGCAACGATCCGCTGGCAATGCAGCGCCTGAAAGAAGCGGCAGAAAAAGCGAAAATCGAACTGTCTTCCGCTCAGCAGACCGACGTTAACCTGCCATACATCACTGCAGACGCGACCGGTCCGAAACACATGAACATCAAAGTGACTCGTGCGAAACTGGAAAGCCTGGTTGAAGATCTGGTAAACCGTTCCATTGAGCCGCTGAAAGTTGCACTGCAGGACGCTGGCCTGTCCGTATCTGATATCGACGACGTTATCCTCGTTGGTGGTCAGACTCGTATGCCAATGGTTCAGAAGAAAGTTGCTGAGTTCTTTGGTAAAGAGCCGCGTA  >  NC_000913/12773‑13249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑